Cellulase Production from Trichoderma harzianum: a Framework Modeling for Evaluating Different Operating Mode Strategies

The cellulase enzyme has been used in essential applications in the textile industry and the formulation of some medicines. Therefore, a mathematical model based on previous reports is proposed for cellulase enzyme enhancement. First, the kinetic constants were taken from the literature. Then, two o...

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Autores:
Mora, D
Carrillo, F
Gelves Gelves, Elizabeth
Tipo de recurso:
Article of investigation
Fecha de publicación:
2021
Institución:
Universidad Francisco de Paula Santander
Repositorio:
Repositorio Digital UFPS
Idioma:
eng
OAI Identifier:
oai:repositorio.ufps.edu.co:ufps/6569
Acceso en línea:
https://repositorio.ufps.edu.co/handle/ufps/6569
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Published under licence by IOP Publishing Ltd
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dc.title.eng.fl_str_mv Cellulase Production from Trichoderma harzianum: a Framework Modeling for Evaluating Different Operating Mode Strategies
title Cellulase Production from Trichoderma harzianum: a Framework Modeling for Evaluating Different Operating Mode Strategies
spellingShingle Cellulase Production from Trichoderma harzianum: a Framework Modeling for Evaluating Different Operating Mode Strategies
title_short Cellulase Production from Trichoderma harzianum: a Framework Modeling for Evaluating Different Operating Mode Strategies
title_full Cellulase Production from Trichoderma harzianum: a Framework Modeling for Evaluating Different Operating Mode Strategies
title_fullStr Cellulase Production from Trichoderma harzianum: a Framework Modeling for Evaluating Different Operating Mode Strategies
title_full_unstemmed Cellulase Production from Trichoderma harzianum: a Framework Modeling for Evaluating Different Operating Mode Strategies
title_sort Cellulase Production from Trichoderma harzianum: a Framework Modeling for Evaluating Different Operating Mode Strategies
dc.creator.fl_str_mv Mora, D
Carrillo, F
Gelves Gelves, Elizabeth
dc.contributor.author.none.fl_str_mv Mora, D
Carrillo, F
Gelves Gelves, Elizabeth
dc.contributor.corporatename.spa.fl_str_mv Journal of Physics: Conference Series
description The cellulase enzyme has been used in essential applications in the textile industry and the formulation of some medicines. Therefore, a mathematical model based on previous reports is proposed for cellulase enzyme enhancement. First, the kinetic constants were taken from the literature. Then, two operating strategies were carried out to maximize cellulase production using 10g/L of cellulose and 0.4g/L of cells as a first strategy using a batch mode. Alternatively, a second operating mode is simulated using continuous cellulose feeding. Finally, a comparative analysis was made for determining the best operating mode related to maximal cellulase production. Interestingly, the continuous mode shows a high cellulase concentration lasting a shorter time than the batch mode to avoid inhibiting enzyme production. One of the most important findings of this work focuses on increasing cellulase production with values of 1800 FPT/L simulating the continuous mode. The latter suggests that cellulase production could be double the concentration obtained with the traditional batch mode (800FPU/L). Results obtained in this research may be promising for the enzyme production industry. Using simulation techniques, it is possible to determine an enhancement without requiring excessive experimentation and resources.
publishDate 2021
dc.date.issued.none.fl_str_mv 2021-09-12
dc.date.accessioned.none.fl_str_mv 2022-11-21T20:47:44Z
dc.date.available.none.fl_str_mv 2022-11-21T20:47:44Z
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
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dc.identifier.uri.none.fl_str_mv https://repositorio.ufps.edu.co/handle/ufps/6569
dc.identifier.doi.none.fl_str_mv 10.1088/1742-6596/2049/1/012093
url https://repositorio.ufps.edu.co/handle/ufps/6569
identifier_str_mv 10.1088/1742-6596/2049/1/012093
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Journal of Physics: Conference Series. Vol. 2049 No.012093(2021)
dc.relation.citationedition.spa.fl_str_mv Vol. 2049 N0.012093 (2021)
dc.relation.citationendpage.spa.fl_str_mv 8
dc.relation.citationissue.spa.fl_str_mv 012093 (2021)
dc.relation.citationstartpage.spa.fl_str_mv 1
dc.relation.citationvolume.spa.fl_str_mv Vol.2049
dc.relation.cites.none.fl_str_mv D Mora et al 2021 J. Phys.: Conf. Ser. 2049 012093
dc.relation.ispartofjournal.spa.fl_str_mv Journal of Physics: Conference Series
dc.rights.eng.fl_str_mv Published under licence by IOP Publishing Ltd
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dc.rights.creativecommons.spa.fl_str_mv Atribución 4.0 Internacional (CC BY 4.0)
rights_invalid_str_mv Published under licence by IOP Publishing Ltd
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Atribución 4.0 Internacional (CC BY 4.0)
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eu_rights_str_mv openAccess
dc.format.extent.spa.fl_str_mv 09 Páginas
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.place.spa.fl_str_mv Reino Unido
dc.source.spa.fl_str_mv https://iopscience.iop.org/article/10.1088/1742-6596/2049/1/012093
institution Universidad Francisco de Paula Santander
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spelling Mora, D61e4eedcf61691dafd6c8f0c1794ab68Carrillo, F8eac5ba13f53e428a2596d530266cae8Gelves Gelves, Elizabeth48702452e3333c2d4cea3b4de2e5bb5f600Journal of Physics: Conference Series2022-11-21T20:47:44Z2022-11-21T20:47:44Z2021-09-12https://repositorio.ufps.edu.co/handle/ufps/656910.1088/1742-6596/2049/1/012093The cellulase enzyme has been used in essential applications in the textile industry and the formulation of some medicines. Therefore, a mathematical model based on previous reports is proposed for cellulase enzyme enhancement. First, the kinetic constants were taken from the literature. Then, two operating strategies were carried out to maximize cellulase production using 10g/L of cellulose and 0.4g/L of cells as a first strategy using a batch mode. Alternatively, a second operating mode is simulated using continuous cellulose feeding. Finally, a comparative analysis was made for determining the best operating mode related to maximal cellulase production. Interestingly, the continuous mode shows a high cellulase concentration lasting a shorter time than the batch mode to avoid inhibiting enzyme production. One of the most important findings of this work focuses on increasing cellulase production with values of 1800 FPT/L simulating the continuous mode. The latter suggests that cellulase production could be double the concentration obtained with the traditional batch mode (800FPU/L). Results obtained in this research may be promising for the enzyme production industry. Using simulation techniques, it is possible to determine an enhancement without requiring excessive experimentation and resources.09 Páginasapplication/pdfengJournal of Physics: Conference Series. Vol. 2049 No.012093(2021)Vol. 2049 N0.012093 (2021)8012093 (2021)1Vol.2049D Mora et al 2021 J. Phys.: Conf. Ser. 2049 012093Journal of Physics: Conference SeriesPublished under licence by IOP Publishing Ltdhttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessAtribución 4.0 Internacional (CC BY 4.0)http://purl.org/coar/access_right/c_abf2https://iopscience.iop.org/article/10.1088/1742-6596/2049/1/012093Cellulase Production from Trichoderma harzianum: a Framework Modeling for Evaluating Different Operating Mode StrategiesArtículo de revistahttp://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttps://purl.org/redcol/resource_type/ECinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Reino UnidoNiño L, Cárdenas A and Gelves G 2013 Evaluation of chemical pretreatments for enzymatic hydrolysis of lignocellulosic residues cassava (Manihot esculenta Crantz). Revista Facultad de Ingeniería 69 317Delabona P, Lima D, Robl D, Rabelo S, Farinas C and Pradella G 2016 Enhanced cellulase production by Trichoderma harzianum by cultivation on glycerol followed by induction on cellulosic substrates. Journal of Industrial Microbiology and Biotechnology 43(5) 617Gelain L, Pradella J and Costa A 2015 Mathematical modeling of enzyme production using Trichoderma harzianum P49P11 and sugarcane bagasse as carbon source. Bioresources Technology 198 101Alvarado K, Bayona J, Consuegra J, Parada D, Sepúlveda N and Gelves G 2020 Use of Operational Training Simulation in the Study of Ethanol Operating Conditions: A Powerful Tool for Education and Research Performance Improvement Journal of Physics: Conference Series 1655 1López L, Peñuela, M and Gelves G 2016 Improving of gas-liquid mass transfer in a stirred tank bioreactor: A CFD approach. International Journal of Applied Engineering. Research 11(9) 6097Niño L and Gelves G 2015 Simulating gas-liquid mass transfer in a spin filter bioreactor. Revista Facultad de Ingeniería 1 163Gutiérrez I, Moreno N and Montoya D 2014 Mecanismos y regulación de la hidrólisis enzimática de celulosa en hongos filamentosos: casos clásicos y nuevos modelos. Revista Iberoamericana de Micología 32(1) 1Llenque L, Muñoz M, Espejo E and Moreno A 2015 Producción de celulasas por Aspergillus niger a partir de bagazo de caña de azúcar en biorreactor aireado. Ciencia y Tecnología 11(1) 32Monod J 1949 The growth of bacterial cultures. Annual Review of Microbiology 3 371Caicedo Y, Suarez C and Gelves G 2020 Evaluation of preliminary plant design for Chlorella vulgaris microalgae production focused on cosmetics purposes Journal of Physics: Conference. Series 1655 012086Ibañez A, Rolon Y and Gelves G 2020 Evaluating Cost-Effective Culture Media for Nutraceutics Production from Microalgae Using Computer-Aided Large Scale Predictions Journal of Physics: Conference. Series 1655 012082Nieto L, Rivera C and Gelves G 202 Economic Assessment of Itaconic Acid Production from Aspergillus Terreus using Superpro Designer Journal of Physics: Conference Series 1655 012100Pacheco S, Niño L and Gelves G 2020 Recombinant Anti-Thrombin Production from Saccharomyces Cerevisiae: Large Scale Trends Based on Computational Predictions Journal of Physics: Conference Series 1655 012081Hernandez S, Niño L and Gelves L 2020 Simulating of Microbial Growth Scale-Up in a Stirred Tank Bioreactor for Aerobic Processes using Computational Fluid Dynamics Journal of Physics: Conference Series 1655 012109LICENSElicense.txtlicense.txttext/plain; charset=utf-814828https://repositorio.ufps.edu.co/bitstream/ufps/6569/2/license.txt2f9959eaf5b71fae44bbf9ec84150c7aMD52open accessORIGINALCellulase Production from Trichoderma harzianum a Framework Modeling for Evaluating Different Operating Mode Strategies.pdfCellulase Production from Trichoderma harzianum a Framework Modeling for 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 incorporada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

7. Término.

a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.

b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.

8. Varios.

a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.

b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.

c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.

d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.
0000-0001-9081-684448702452e3333c2d4cea3b4de2e5bb5f600