Production of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale

Rice husk (RH) is the main biomass byproduct of the rice industry, so much that it corresponds to 20% of the total cereal production and is generally considered a waste. Due to its content of cellulose and hemicellulose, the RH has a great potential for its alternative use in the production of bioet...

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Autores:
Cacua Silva, Alejandra
Rodriguez Ordonez, Dora Cecilia
Parra LLanos, John Wilmer
Tipo de recurso:
Article of journal
Fecha de publicación:
2018
Institución:
Universidad Francisco de Paula Santander
Repositorio:
Repositorio Digital UFPS
Idioma:
eng
OAI Identifier:
oai:repositorio.ufps.edu.co:ufps/968
Acceso en línea:
http://repositorio.ufps.edu.co/handle/ufps/968
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Rights
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License
Atribución 4.0 Internacional (CC BY 4.0)
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dc.title.eng.fl_str_mv Production of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale
title Production of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale
spellingShingle Production of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale
title_short Production of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale
title_full Production of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale
title_fullStr Production of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale
title_full_unstemmed Production of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale
title_sort Production of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale
dc.creator.fl_str_mv Cacua Silva, Alejandra
Rodriguez Ordonez, Dora Cecilia
Parra LLanos, John Wilmer
dc.contributor.author.none.fl_str_mv Cacua Silva, Alejandra
Rodriguez Ordonez, Dora Cecilia
Parra LLanos, John Wilmer
description Rice husk (RH) is the main biomass byproduct of the rice industry, so much that it corresponds to 20% of the total cereal production and is generally considered a waste. Due to its content of cellulose and hemicellulose, the RH has a great potential for its alternative use in the production of bioethanol, since it has a great advantage with respect to the conventional raw materials used (sugar cane, corn, beet and sweet sorghum, among others), given that this lignocellulosic material is abundant and does not compete with the food industry. The objective of this work was to obtain bioethanol from rice husk at a pilot scale, taking advantage of its cellulose and hemicellulose content. For this, a sample of 450g of RH was taken, which was pretreated with a 2.00% w/v NaOH solution and subsequently hydrolyzed with acid cellulase (CFB3S). The alcoholic fermentation of total reducing sugars (TRS) obtained in the enzymatic hydrolysis was carried out with Saccharomyces cerevisiae, finally, the fractional distillation of the must was carried out, by means of which four bioethanol samples were obtained with concentrations of 71.3% v/v, 30.0% v/v, 10.3% v/v, and 5.3% v/v of alcohol, which when mixed produced a sample of 95mL of solution with a concentration of 27% v/v alcohol (4.70g/100g of RH).
publishDate 2018
dc.date.issued.none.fl_str_mv 2018-12-07
dc.date.accessioned.none.fl_str_mv 2021-11-14T16:04:07Z
dc.date.available.none.fl_str_mv 2021-11-14T16:04:07Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.identifier.uri.none.fl_str_mv http://repositorio.ufps.edu.co/handle/ufps/968
dc.identifier.doi.none.fl_str_mv 10.1088/1742-6596/1126/1/012034
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dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Journal of Physics: Conference Series
dc.relation.citationedition.spa.fl_str_mv Vol.1126 No.1.(2019)
dc.relation.citationendpage.spa.fl_str_mv 7
dc.relation.citationissue.spa.fl_str_mv 1 (2018)
dc.relation.citationstartpage.spa.fl_str_mv 1
dc.relation.citationvolume.spa.fl_str_mv 1126
dc.relation.cites.none.fl_str_mv Cacua, A., Gelvez, J. J., Rodriguez, D. C., & Parra, J. W. (2018, November). Production of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale. In Journal of Physics: Conference Series (Vol. 1126, No. 1, p. 012034). IOP Publishing.
dc.relation.ispartofjournal.spa.fl_str_mv Journal of Physics: Conference Series
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dc.publisher.spa.fl_str_mv Journal of Physics: Conference Series
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spelling Cacua Silva, Alejandra6db0ca567917bbe533b54b5edd119be1Rodriguez Ordonez, Dora Cecilia1c8638f52e9d867a2240380d274c2b28Parra LLanos, John Wilmer71c25954e9b94ea54af9ebf0502af0aa6002021-11-14T16:04:07Z2021-11-14T16:04:07Z2018-12-07http://repositorio.ufps.edu.co/handle/ufps/96810.1088/1742-6596/1126/1/012034Rice husk (RH) is the main biomass byproduct of the rice industry, so much that it corresponds to 20% of the total cereal production and is generally considered a waste. Due to its content of cellulose and hemicellulose, the RH has a great potential for its alternative use in the production of bioethanol, since it has a great advantage with respect to the conventional raw materials used (sugar cane, corn, beet and sweet sorghum, among others), given that this lignocellulosic material is abundant and does not compete with the food industry. The objective of this work was to obtain bioethanol from rice husk at a pilot scale, taking advantage of its cellulose and hemicellulose content. For this, a sample of 450g of RH was taken, which was pretreated with a 2.00% w/v NaOH solution and subsequently hydrolyzed with acid cellulase (CFB3S). The alcoholic fermentation of total reducing sugars (TRS) obtained in the enzymatic hydrolysis was carried out with Saccharomyces cerevisiae, finally, the fractional distillation of the must was carried out, by means of which four bioethanol samples were obtained with concentrations of 71.3% v/v, 30.0% v/v, 10.3% v/v, and 5.3% v/v of alcohol, which when mixed produced a sample of 95mL of solution with a concentration of 27% v/v alcohol (4.70g/100g of RH).application/pdfengJournal of Physics: Conference SeriesJournal of Physics: Conference SeriesVol.1126 No.1.(2019)71 (2018)11126Cacua, A., Gelvez, J. J., Rodriguez, D. C., & Parra, J. W. (2018, November). Production of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale. In Journal of Physics: Conference Series (Vol. 1126, No. 1, p. 012034). IOP Publishing.Journal of Physics: Conference SeriesContent from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. Published under licence by IOP Publishing Ltdinfo:eu-repo/semantics/openAccessAtribución 4.0 Internacional (CC BY 4.0)http://purl.org/coar/access_right/c_abf2https://iopscience.iop.org/article/10.1088/1742-6596/1126/1/012034/metaProduction of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scaleArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Riaño A, Morales A, Hernández J and Barrero C 2010 Producción de bioetanol a partir de subproductos agroindustriales lignocelulósicos Tumbaga 1 61-91Vargas J, Alvarado P, Vega-Baudrit J and Porras M 2013 Caracterización del subproducto cascarillas de arroz en búsqueda de posibles aplicaciones como materia prima en procesos Revista Científica 23 1-15Fuentes C, Segundo A, Granados J and Piedrahita W 2006 Flora arvense asociada con el cultivo del arroz en el departamento del Tolima-Colombia (Bogotá: Universidad Nacional de Colombia)Proaños J and Castro Y 2014 Evaluación de la producción de ácido láctico a partir de cascarilla de arroz por Lactobacillus del brueckii Revista Mutis 4 7-18Nachaiwieng W, Lumyong S, Yoshioka K, Watanabe T and Khanongnuch C 2015 Bioethanol production from rice husk under elevated temperature simultaneous saccharification and fermentation using Kluyveromyces marxianus CK8 Biocatalysis and Agricultural Biotechnology 4 543-549Zhang Q and Cai W 2008 Enzymatic hydrolysis of alkali-pretreated rice straw by Trichoderma reesei ZM4-F3 Biomass and Bioenergy 32 1130-1135Zhang Q, He G, Wang J, Cai W and Xu Y 2009 Two-stage co-hydrolysis of rice straw by Trichoderma reesei ZM4-F3 and Pseudomonas aeruginosa BSZ-07 Biomass and Bioenergy 33 1464-1468Collaguazo Simbaña P E 2011 Estudio de la tecnología para la obtención de una bebida alcohólica carbonatada a partir de la degradación de la cascarilla de arroz (oryza sativa) tratada enzimáticamente con hemicelulasa (Ambato: Universidad Técnica de Ambato Ecuador)Ordóñez Y and Tannous K 2017 Análisis cinético de la descomposición térmica de biomasas aplicando un esquema de reacciones paralelas independientes Revista UIS Ingenierías 16 119-28Uribe N, Echeverry C, Betancur M, Zapata L and Martínez J 2018 Possibilities of rice husk ash to be used as reinforcing filler in polymer sector-a review Revista UIS Ingenierías 17 127-42Piñeros Y and Otálvaro A 2010 VIII Congreso Nacional de Biotecnología y Bioingeniería, VII Simposio Internacional de Producción de Alcoholes y levaduras (México: Sociedad Mexicana de Biotecnología y Bioingeniería) Evaluación de la producción de etanol a partir de cascarilla de arroz pre-tratada con NaClO, mediante hidrólisis y fermentación simultaneasKarlson P 2014 Introduction to Modern Biochemistry (California: Elsevier Science)Montenegro Vega Y N and Peñaranda Fuentes A 2015 Obtención de etanol a partir de residuos de madera utilizando sistema biológico (Aspergillus niger - Saccharomyces cerevisiae) con pretratamiento con ultrasonido (Cucuta: Universidad Francisco de Paula Santander)Benítez L, Tovar C, Ortiz Á, Dunoyer A, Alvear M and Castillo C 2010 Producción de bioetanol a partir de la fermentación alcohólica de jarabes glucosados derivados de cáscaras de naranja y piña Revista. Educación en Ingeniería 5 120-125Argote F E, Cuervo R A, Osorio E, Delgado J and Villada H S 2015 Evaluación de la Producción de Etanol a partir de melaza con cepas nativas saccharomyces cerevisiae Revista Biotecnología en el Sector Agropecuario y Agroindustrial 13 40-48ORIGINALProduction of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale.pdfProduction of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale.pdfapplication/pdf536918https://repositorio.ufps.edu.co/bitstream/ufps/968/1/Production%20of%20bioethanol%20from%20rice%20husk%20pretreated%20with%20alkalis%20and%20hydrolyzed%20with%20acid%20cellulase%20at%20pilot%20scale.pdf49c1d0a8260ab6c338f8e4e8bdc8214bMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-814828https://repositorio.ufps.edu.co/bitstream/ufps/968/2/license.txt2f9959eaf5b71fae44bbf9ec84150c7aMD52open accessTEXTProduction of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale.pdf.txtProduction of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale.pdf.txtExtracted texttext/plain27530https://repositorio.ufps.edu.co/bitstream/ufps/968/3/Production%20of%20bioethanol%20from%20rice%20husk%20pretreated%20with%20alkalis%20and%20hydrolyzed%20with%20acid%20cellulase%20at%20pilot%20scale.pdf.txt035c37d0ef218ae34befe6569852ededMD53open accessTHUMBNAILProduction of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale.pdf.jpgProduction of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale.pdf.jpgGenerated 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 incorporada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

7. Término.

a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.

b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.

8. Varios.

a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.

b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.

c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.

d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.
