Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa)
Milax gagates is one of the main pests in the cultivation of lettuce, causing considerable economic losses. Control with molluscicides totally or partially eliminates the pest but generates a negative impact on the environment due to their high toxicity. Biological management also allows controlling...
- Autores:
-
Galvis, Fabian
Moreno Rozo, laura Yolima
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad Francisco de Paula Santander
- Repositorio:
- Repositorio Digital UFPS
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.ufps.edu.co:ufps/1782
- Acceso en línea:
- http://repositorio.ufps.edu.co/handle/ufps/1782
http://dx.doi.org/10.5154/r.rchsh.2017.07.025
- Palabra clave:
- biological control
slugs
molluscicides
bioassays
control biológico
babosas
molusquicidas
bioensayos
- Rights
- openAccess
- License
- Atribución 4.0 Internacional (CC BY 4.0)
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Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa) |
| dc.title.translated.none.fl_str_mv |
Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa) |
| title |
Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa) |
| spellingShingle |
Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa) biological control slugs molluscicides bioassays control biológico babosas molusquicidas bioensayos |
| title_short |
Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa) |
| title_full |
Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa) |
| title_fullStr |
Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa) |
| title_full_unstemmed |
Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa) |
| title_sort |
Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa) |
| dc.creator.fl_str_mv |
Galvis, Fabian Moreno Rozo, laura Yolima |
| dc.contributor.author.none.fl_str_mv |
Galvis, Fabian Moreno Rozo, laura Yolima |
| dc.subject.proposal.eng.fl_str_mv |
biological control slugs molluscicides bioassays |
| topic |
biological control slugs molluscicides bioassays control biológico babosas molusquicidas bioensayos |
| dc.subject.proposal.spa.fl_str_mv |
control biológico babosas molusquicidas bioensayos |
| description |
Milax gagates is one of the main pests in the cultivation of lettuce, causing considerable economic losses. Control with molluscicides totally or partially eliminates the pest but generates a negative impact on the environment due to their high toxicity. Biological management also allows controlling the pest, but in a specific and safe way for the environment. Therefore, the objective of this work was to evaluate the toxic effect of Bacillus thuringiensis, with Cry1 genes, against M. gagates by means of a bioassay. Seventy-five soil samples were taken from several municipalities in Norte de Santander, Colombia, from where 58 isolated colonies of B. thuringiensis with similar characteristics were obtained. Of these colonies, five showed the presence of Cry1 genes, and were those used in the bioassays together with the control B. thuringiensis var. Kurstaki. The treatments caused 90-100 % lethality to M. gagates at a concentration of 500 μg∙mL-1. The calculated LD99 suggests the use of 820 μg∙mL-1 of the biopreparate to obtain 100 % lethality with any of the five isolates studied. |
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2018 |
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2018-08 |
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2021-12-09T21:14:21Z |
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2021-12-09T21:14:21Z |
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Artículo de revista |
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http://dx.doi.org/10.5154/r.rchsh.2017.07.025 |
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eng |
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eng |
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Revista Chapingo Serie Horticultura |
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Vol.24 No.2.(2018) |
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Galvis, F., & Moreno, L. (2018). Identification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates, a pest on lettuce (Lactuca sativa). Revista Chapingo. Serie horticultura, 24(2), 97-106. |
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Atribución 4.0 Internacional (CC BY 4.0) |
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Atribución 4.0 Internacional (CC BY 4.0) http://purl.org/coar/access_right/c_abf2 |
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Revista Chapingo Serie Horticultura |
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Ciudad de Mexico , Mexico |
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Galvis, Fabian508e26c4055abdc301fd2290492a16d0600Moreno Rozo, laura Yolimacd8cd47d230d6dd4dd89b3e975c18e196002021-12-09T21:14:21Z2021-12-09T21:14:21Z2018-08http://repositorio.ufps.edu.co/handle/ufps/1782http://dx.doi.org/10.5154/r.rchsh.2017.07.025Milax gagates is one of the main pests in the cultivation of lettuce, causing considerable economic losses. Control with molluscicides totally or partially eliminates the pest but generates a negative impact on the environment due to their high toxicity. Biological management also allows controlling the pest, but in a specific and safe way for the environment. Therefore, the objective of this work was to evaluate the toxic effect of Bacillus thuringiensis, with Cry1 genes, against M. gagates by means of a bioassay. Seventy-five soil samples were taken from several municipalities in Norte de Santander, Colombia, from where 58 isolated colonies of B. thuringiensis with similar characteristics were obtained. Of these colonies, five showed the presence of Cry1 genes, and were those used in the bioassays together with the control B. thuringiensis var. Kurstaki. The treatments caused 90-100 % lethality to M. gagates at a concentration of 500 μg∙mL-1. The calculated LD99 suggests the use of 820 μg∙mL-1 of the biopreparate to obtain 100 % lethality with any of the five isolates studied.Milax gagates constituye una de las principales plagas en el cultivo de lechuga causando considerables pérdidas económicas. El control con molusquicidas elimina total o parcialmente la plaga, pero genera un impacto negativo en el ambiente por su alta toxicidad. El manejo biológico también permite el control de la plaga, pero de manera específica e inocua para el ambiente. Por ello, el objetivo de este trabajo fue evaluar el efecto tóxico de Bacillus thuringiensis, con genes Cry1, contra M. gagates mediante la elaboración de un bioensayo. Se tomaron 75 muestras de suelo de varios municipios del Norte de Santander, Colombia, de donde se obtuvieron 58 colonias aisladas de B. thuringiensis con características similares. De estas colonias, cinco evidenciaron la presencia de genes Cry1, y fueron las empleadas en los bioensayos junto con el control B. thuringiensis var. Kurstaki. Los tratamientos presentaron letalidad ante M. gagates de entre 90 y 100 % a una concentración de 500 μg∙mL-1. La CL99 calculada sugiere el uso de 820 μg∙mL-1 del biopreparado para obtener 100 % de letalidad con cualquiera de los cinco aislados estudiadosapplication/pdfengRevista Chapingo Serie HorticulturaCiudad de Mexico , MexicoRevista Chapingo Serie HorticulturaVol.24 No.2.(2018)1062 (2018)9724Galvis, F., & Moreno, L. (2018). Identification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates, a pest on lettuce (Lactuca sativa). Revista Chapingo. Serie horticultura, 24(2), 97-106.Revista Chapingo Serie HorticulturaThis is an open-access article distributed under the terms of the Creative Commons Attribution License view the permissions of this licenseinfo:eu-repo/semantics/openAccessAtribución 4.0 Internacional (CC BY 4.0)http://purl.org/coar/access_right/c_abf2https://revistas.chapingo.mx/horticultura/?section=articles&subsec=issues&numero=255&articulo=2479Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa)Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa)Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85biological controlslugsmolluscicidesbioassayscontrol biológicobabosasmolusquicidasbioensayosAbd-El-Ghany, A. M., & Abd-El-Ghany, N. M. (2017). Molluscicidal activity of Bacillus thuringiensis strains against Biomphalaria alexandrina snails. Beni-Suef University Journal of Basic and Applied Sciences, 6(4), 1-3. doi: 10.1016/j.bjbas.2017.05.003Arrieta, G., Hernández, A., & Espinoza, A. M. (2004). Diversity of Bacillus thuringiensis strains isolated from coffee plantations infested with the coffee berry borer Hypothenemus hampei. Revista de Biología Tropical, 52(3), 757-764. Retrieved from http://www.scielo.sa.cr/scielo.php?pid=S0034-77442004000300036&script=sci_arttextBravo, A., Pacheco, S., Gómez, I., García-Gómez, B., Onofre, J., & Soberón, M. (2017). Insecticidal proteins from Bacillus thuringiensis and their mechanism of action. In: Fiuza, L., Polanczyk, R., & Crickmore, N. (Eds), Bacillus thuringiensis and Lysinibacillus sphaericus (pp. 53-66). Cham, Switzerland: Springer International Publishing. doi: 10.1007/978-3-319-56678-8_4Carmona, A. (2002). Aislamiento y caracterización parcial de una cepa de Bacillus thurigiensis tóxica a Spodoptera frugiperda (Lepidoptera: Noctuidae). Bioagro, 14(1), 3-10. Retrieved from http://www.redalyc.org/pdf/857/85714101.pdfCórdoba-Vargas, C. A., & León-Sicard, T. (2010). Efecto del manejo agroecológico y convencional sobre la fluctuación de babosa en cultivos de lechuga en Tenjo Cundinamarca. Acta Biológica Colombiana, 15(1), 115. doi: 10.15446/abcCrickmore, N. (2017). Bacillus thuringiensis toxin classification. In: Fiuza, L., Polanczyk, R., & Crickmore, N. (Eds), Bacillus thuringiensis and Lysinibacillus sphaericus (pp. 41-52). Cham, Switzerland: Springer International Publishing . doi: 10.1007/978-3-319-56678-8_3Crickmore, N., Bone, E. J., Williams, J. A., & Ellar, D. J. (1995). Contribution of the individual components of the δ-endotoxin crystal to the mosquitocidal activity of Bacillus thuringiensis subsp. israelensis. FEMS Microbiology Letters, 131(3), 249-254. doi: 10.1111/j.1574-6968.1995.tb07784.xDjenane, Z., Nateche, F., Amziane, M., Gomis-Cebolla, J., El-Aichar, F., Khorf, H., & Ferré, J. (2017). Assessment of the antimicrobial activity and the entomocidal potential of Bacillus thuringiensis isolates from Algeria. Toxins, 9(4), 139-158. doi: 10.3390/toxins9040139Finney, D. J. (1971). Probit Analysis (pp. 68-72). Cambridge: Cambridge University Press.Food and Agriculture Organization Corporate Statistical Database (FAOSTAT). (2014). FAO statistics division 2014. Retrieved from http://faostat.fao.org/France, A., Gerding, M., Céspedes, C., & Cortez, M. (2002). Control de babosas (Deroceras reticulatum Müller) con Phasmarhabditis hermaphrodita Schneider (Nematoda: Rhabditidae) en suelos con sistema de cero labranza. Agricultura Técnica, 62(2), 181-190. doi: 10.4067/S0365-28072002000200001Galvis-Serrano, F. (2013). Caracterización molecular de genes cry1, cry2, cry3 y cry4 en aislados de Bacillus thuringiensis y determinación de su actividad bioinsecticida en larvas de Aedes aegypti. Interciencia, 38(2), 128-131. Retrieved from http://www.redalyc.org/html/339/33926950004/Gao, M., Li, R., Dai, S., Wu, Y., & Yi, D. (2008). Diversity of Bacillus thuringiensis strains from soil in China and their pesticidal activities. Biological Control, 44(3), 380-388. doi: 10.1016/j.biocontrol.2007.11.011Garavano, M. E., Manetti, P. L., López, A. N., Clemente, N. L., Salvio, C., & Faberi, A. J. (2013). Cebos molusquicidas y molusquicidas líquidos para el control de Deroceras reticulatum (Pulmonata: Stylomatophora), plaga en el cultivo de colza. Revista de Investigaciones Agropecuarias, 39(1), 60-66. Retrieved from http://www.redalyc.org/articulo.oa?id=86426063006Godan, D. (1983). Pest slugs and snails. Biology and control (pp. 127-132). Berlin: Springer-Verlag.Gorashi, N. E., Tripathi, M., Kalia, V., & Gujar, G. T. (2014). Identification and characterization of the Sudanese Bacillus thuringiensis and related bacterial strains for their efficacy against Helicoverpa armigera and Tribolium castaneum. Indian Journal of Experimental Biology, 52(6), 637-649. Retrieved from https://pdfs.semanticscholar.org/90b8/534a663e65b296ad19ff9885e7bb57839406.pdfHung, T. P., Truong, L. V., Binh, N. D., Frutos, R., Quiquampoix, H., & Staunton, S. (2016). Fate of insecticidal Bacillus thuringiensis Cry protein in soil: differences between purified toxin and biopesticide formulation. Pest Management Science, 72(12), 2247-2253. doi: 10.1002/ps.4262Ibarra, J. E., del Rincón, M. C., Ordúz, S., Noriega, D., Benintende, G., Monnerat, R., & Sánchez, J. (2003). Diversity of Bacillus thuringiensis strains from Latin America with insecticidal activity against different mosquito species. Applied and Environmental Microbiology, 69(9), 5269-5274. doi: 10.1128/AEM.69.9.5269-5274.2003IBM SPSS. (2014). Statistics for Windows ver. 23. Retrieved from https://www.ibm.com/us-en/Jain, D., Sunda, S. D., Sanadhya, S., Nath, D. J., & Khandelwal, S. K. (2017). Molecular characterization and PCR-based screening of cry genes from Bacillus thuringiensis strains. Biotech, 7(4), 1-8. doi: 10.1007/s13205-016-0583-7Khodabandeh, F., Safaralizadeh, M. H., Safavi, S. A., & Aramideh, S. (2014). Virulence of some native Bacillus thuringiensis isolates against Ephestia kuehniella (Zeller) (Lep., Pyralidae) and Pieris brassicae (Lep., Pieridae) larvae isolated from stored products of Urmia city. Archives of Phytopathology and Plant Protection, 47(5), 610-614. doi: 10.1080/03235408.2013.816101Kienlen, J. C., Gertz, C., Briard, P., Hommay, G., & Chaufaux, J. (1996). Recherche de la toxicité de diverses souches de Bacillus thuringiensis Berliner vis-à-vis de trois espèces de limaces. Agronomie, 16(6), 347-353. doi: 10.1051/agro:19960602Monnerat, R. G., Batista, A. C., de Medeiros, P. T., Martins, E. S., Melatti, V. M., Praça, L. B., & Falcao, R. (2007). Screening of Brazilian Bacillus thuringiensis isolates active against Spodoptera frugiperda, Plutella xylostella and Anticarsia gemmatalis. Biological Control, 41(3), 291-295. doi: 10.1016/j.biocontrol.2006.11.008Neira, O., Muñoz, S., Stanley, V., Gosh, C., & Rosales, L. (2010). Cryptosporidium parvum in wild gastropods as bioindicators of fecal contamination in terrestrial ecosystems. Revista Chilena de Infectología, 27(3), 211-218. doi: 10.4067/S0716-10182010000300006Osman, G. Y., & Mohamed, A. M. (1991). Bio-efficacy of bacterial insecticide, Bacillus thuringiensis Berl. as biological control agent against snails vectors of Schistosomiasis in Egypt. Anzeiger für Schädlingskunde, 64(7), 136-139. doi: 10.1007/BF01906005Peña, G., Miranda-Rios, J., de la Riva, G., Pardo-López, L., Soberón, M., & Bravo, A. (2006). A Bacillus thuringiensis S-layer protein involved in toxicity against Epilachna varivestis (Coleoptera: Coccinellidae). Applied and environmental microbiology, 72(1), 353-360. doi: 10.1128/AEM.72.1.353-360.2006Pitre, L., Hernández-Fernández, J., & Bernal, J. (2008). Toxicidad de δ-endotoxinas recombinantes de Bacillus thuringiensis sobre larvas de la polilla guatemalteca (Tecia solanivora) (Lepidóptera: Gelechiidae). Revista Colombiana de Biotecnología, 10(2), 85-96.Rojas-Arias, A. C., López-Pazos, S. A., & Chaparro-Giraldo, A. (2013). Actividad biológica de Bacillus thuringiensis sobre la polilla guatemalteca de la papa, Tecia solanivora Povolny (Lepidoptera: Gelechiidae). Revista Mutis, 3(2), 31-42. doi: 10.21789/22561498.883Salama, H. S., Abd-El-Ghany, N. A., & Saker, M. M. (2015). Diversity of Bacillus thuringiensis isolates from Egyptian soils as shown by molecular characterization. Journal of Genetic Engineering and Biotechnology, 13(2), 101-109. doi: 10.1016/j.jgeb.2015.10.001Schünemann, R., Knaak, N., & Fiuza, L. M. (2014). Mode of action and specificity of Bacillus thuringiensis toxins in the control of caterpillars and stink bugs in soybean culture. ISRN Microbiology, 2014, 1-13. doi: 10.1155/2014/135675Serre, M. (2005). Manejo de babosas en el cultivo de girasol en siembra directa. Argentina: Pioneer Argentina. Retrieved from http://www.pioneer.com/CMRoot/International/Argentina_Intl/AGRONOMIA/con_agric_inv_lotes/IL_Manejobabosa_girasol_05.pdfVan Frankenhuyzen, K. (2013). Cross-order and cross-phylum activity of Bacillus thuringiensis pesticidal proteins. Journal of Invertebrate Pathology, 114(1), 76-85. doi: 10.1016/j.jip.2013.05.010Vargas-Arcila, M., Cartagena-Valenzuela, J. R., Franco, G., Correa-Londoño, G. A., Quintero-Vásquez, L. M., & Gaviria-Montoya, C. A. (2017). Changes in the physico-chemical properties of four lettuce (Lactuca sativa L.) varieties during storage. Corpoica Cienc Tecnol Agropecuaria, 18(2), 257-273. doi: 10.21930/rcta.vol18_num2_art:632257-273Vázquez-Ramírez, M. F., Rangel-Núñez, J. C., Ibarra, J. E., & del Rincón-Castro, M. C. (2015). Evaluación como agentes de control biológico y caracterización de cepas mexicanas de Bacillus thuringiensis contra el gusano cogollero del maíz Spodoptera frugiperda (Lepidotera: Noctuidae). Interciencia, 40(6). Retrieved from http://www.redalyc.org/pdf/339/33938675006.pdfZothansanga, L., Kumar, N. S., & Gurusubramanian, G. (2011). PCR pathotyping of native Bacillus thuringiensis from Mizoram, India. Sciense Vision, 11(3), 171-176. Retrieved from https://pdfs.semanticscholar.org/0b59/466b7c801f3abc8308c34f4f39e9b7130eba.pdfZurbrügg, C., & Nentwig, W. (2009). Ingestion and excretion of two transgenic Bt corn varieties by slugs. Transgenic Research, 18(2), 215-225. doi: 10.1007/s11248-008-9208-1ORIGINALIdentificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa).pdfIdentificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa).pdfapplication/pdf1290492https://repositorio.ufps.edu.co/bitstream/ufps/1782/1/Identificaci%c3%b3n%20de%20genes%20Cry1%20en%20aislados%20de%20Bacillus%20thuringiensis%20y%20su%20efecto%20t%c3%b3xico%20contra%20Milax%20gagates%2c%20plaga%20en%20lechuga%20%28Lactuca%20sativa%29.pdf19f1be18878b0d9dec4b892386fce44cMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-814828https://repositorio.ufps.edu.co/bitstream/ufps/1782/2/license.txt2f9959eaf5b71fae44bbf9ec84150c7aMD52open accessTEXTIdentificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca 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Thumbnailimage/jpeg13315https://repositorio.ufps.edu.co/bitstream/ufps/1782/4/Identificaci%c3%b3n%20de%20genes%20Cry1%20en%20aislados%20de%20Bacillus%20thuringiensis%20y%20su%20efecto%20t%c3%b3xico%20contra%20Milax%20gagates%2c%20plaga%20en%20lechuga%20%28Lactuca%20sativa%29.pdf.jpgfe3723076c4c2ae83f2b41f0c7f71bb3MD54open accessufps/1782oai:repositorio.ufps.edu.co:ufps/17822022-05-23 10:43:19.931open accessRepositorio Universidad Francisco de Paula 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 incorporada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

7. Término.

a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.

b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.

8. Varios.

a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.

b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.

c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.

d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.
0000-0002-0026-4829508e26c4055abdc301fd2290492a16d06000000-0001-8903-671Xcd8cd47d230d6dd4dd89b3e975c18e19600 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