Molecular analysis of antagonist fungi isolated from plantations of cocoa (Theobroma cacao) from north of Santander
Molecular biology techniques have been used in order to obtain specific species genetic markers, find polymorphic differences and provide the necessary information for the identification of nucleotide sequences related to pathogenicity factors in species related to biological control. At present, bi...
- Autores:
-
López Barrera, German Luciano
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2021
- Institución:
- Universidad Francisco de Paula Santander
- Repositorio:
- Repositorio Digital UFPS
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.ufps.edu.co:ufps/302
- Acceso en línea:
- http://repositorio.ufps.edu.co/handle/ufps/302
- Palabra clave:
- Antagonist
Biotechnology
Genetic diversity
PCR
RAPD
Antagonista
Biotecnología
Diversidad genética
PCR
RAPD
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/4.0/
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Molecular analysis of antagonist fungi isolated from plantations of cocoa (Theobroma cacao) from north of Santander |
| dc.title.spa.fl_str_mv |
Análisis molecular de hongos antagonistas aislados de plantaciones de cacao (Theobroma cacao) de Norte de Santander |
| title |
Molecular analysis of antagonist fungi isolated from plantations of cocoa (Theobroma cacao) from north of Santander |
| spellingShingle |
Molecular analysis of antagonist fungi isolated from plantations of cocoa (Theobroma cacao) from north of Santander Antagonist Biotechnology Genetic diversity PCR RAPD Antagonista Biotecnología Diversidad genética PCR RAPD |
| title_short |
Molecular analysis of antagonist fungi isolated from plantations of cocoa (Theobroma cacao) from north of Santander |
| title_full |
Molecular analysis of antagonist fungi isolated from plantations of cocoa (Theobroma cacao) from north of Santander |
| title_fullStr |
Molecular analysis of antagonist fungi isolated from plantations of cocoa (Theobroma cacao) from north of Santander |
| title_full_unstemmed |
Molecular analysis of antagonist fungi isolated from plantations of cocoa (Theobroma cacao) from north of Santander |
| title_sort |
Molecular analysis of antagonist fungi isolated from plantations of cocoa (Theobroma cacao) from north of Santander |
| dc.creator.fl_str_mv |
López Barrera, German Luciano |
| dc.contributor.author.none.fl_str_mv |
López Barrera, German Luciano |
| dc.subject.proposal.eng.fl_str_mv |
Antagonist Biotechnology Genetic diversity PCR RAPD |
| topic |
Antagonist Biotechnology Genetic diversity PCR RAPD Antagonista Biotecnología Diversidad genética PCR RAPD |
| dc.subject.proposal.spa.fl_str_mv |
Antagonista Biotecnología Diversidad genética PCR RAPD |
| description |
Molecular biology techniques have been used in order to obtain specific species genetic markers, find polymorphic differences and provide the necessary information for the identification of nucleotide sequences related to pathogenicity factors in species related to biological control. At present, biological control is considered indispensable in the strategies of sustainable agriculture with agroecological base in crops, growing interest in Colombia such as cocoa (Theobroma cacao L.). Molecularly analyze different strains of antagonistic fungi with biotechnological potential in order to elucidate genomic similarities and the search for DNA fragments related to pathogenicity factors. The technique of RAPD (Randomized Amplified Polymorphic DNA) was standardized, using 10 primers, and the pr1 gene was amplified using the PCR technique using 6 pairs of primers. An initial classification of the population of antagonistic fungi was achieved and the diversity of the isolates was also demonstrated. In addition, three genes of the Beauveria bassiana, Trichoderma yunnanense and Purpureocillium lilacinus strains were amplified, which were found to encode a family of proteases designed to exert antagonistic activity against a wide range of hosts. It can be inferred that the strains under study are good initial candidates for the formulation of biopreparations destined to the biological control of plague organisms in crops of agricultural interest in the region, due to the fact that traits and genes were found that will allow to exert a wide antagonistic action. |
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2021 |
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2021-10-13T20:18:43Z |
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2021-10-13T20:18:43Z |
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2021-05-18 |
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Artículo de revista |
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10.25100/iyc.23i2.11154 |
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Revista Ingeniería Y Competitividad ISSN: 2027-8284, 2021 vol:23 fasc: N/A págs: 1 - 11, DOI:10.25100/iyc.23i2.11154 |
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vol.23 no.2 (2021) |
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11 |
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López-Barrera GL. Análisis molecular de hongos antagonistas aislados de plantaciones de cacao (Theobroma cacao) de Norte de Santander. INGENIERÍA Y COMPETITIVIDAD, 23(2), In press 2021. https://doi.org/10.25100/iyc.v23i2.11154. López-Barrera GL. Molecular analysis of antagonist fungi isolated from plantations of cocoa (Theobroma cacao) from north of Santander. INGENIERÍA Y COMPETITIVIDAD, 23(2), In press 2021. https://doi.org/10.25100/iyc.v23i2.11154. |
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Revista Ingeniería Y Competitividad |
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11 páginas |
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Ingeniería Y Competitividad |
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Colombia |
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Ingeniería Y Competitividad |
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https://revistaingenieria.univalle.edu.co/index.php/ingenieria_y_competitividad/article/view/11154 |
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Universidad Francisco de Paula Santander |
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López Barrera, German Luciano332ac6b52127580d0633e118d7f54cb86002021-10-13T20:18:43Z2021-10-13T20:18:43Z2021-05-18http://repositorio.ufps.edu.co/handle/ufps/30210.25100/iyc.23i2.11154Molecular biology techniques have been used in order to obtain specific species genetic markers, find polymorphic differences and provide the necessary information for the identification of nucleotide sequences related to pathogenicity factors in species related to biological control. At present, biological control is considered indispensable in the strategies of sustainable agriculture with agroecological base in crops, growing interest in Colombia such as cocoa (Theobroma cacao L.). Molecularly analyze different strains of antagonistic fungi with biotechnological potential in order to elucidate genomic similarities and the search for DNA fragments related to pathogenicity factors. The technique of RAPD (Randomized Amplified Polymorphic DNA) was standardized, using 10 primers, and the pr1 gene was amplified using the PCR technique using 6 pairs of primers. An initial classification of the population of antagonistic fungi was achieved and the diversity of the isolates was also demonstrated. In addition, three genes of the Beauveria bassiana, Trichoderma yunnanense and Purpureocillium lilacinus strains were amplified, which were found to encode a family of proteases designed to exert antagonistic activity against a wide range of hosts. It can be inferred that the strains under study are good initial candidates for the formulation of biopreparations destined to the biological control of plague organisms in crops of agricultural interest in the region, due to the fact that traits and genes were found that will allow to exert a wide antagonistic action.Las técnicas de biología molecular se han utilizado con el fin de obtener marcadores genéticos especie específicos, encontrar diferencias polimórficas y proporcionar la información necesaria para la identificación de secuencias nucleotídicas relacionadas con factores de patogenicidad en especies relacionadas con el control biológico. En la actualidad el control biológico se considera indispensable en las estrategias de agricultura sostenible con base agroecológica en los cultivos interés creciente en Colombia como el cacao (Theobroma cacao L.). Analizar molecularmente diferentes cepas de hongos antagonistas con potencial biotecnológico con el fin de dilucidar similitudes genómicas y la búsqueda de fragmentos de ADN relacionados con factores de patogenicidad. Se estandarizó la técnica de RAPDs, mediante el uso de 10 iniciadores, así mismo se amplificó el gen pr1 mediante la técnica de PCR usando 6 pares de iniciadores. Se logró una clasificación inicial de la población de hongos antagonistas y así mismo se demostró la diversidad de los aislados. Además, se logró amplificar tres genes de las cepas Beauveria bassiana, Trichoderma yunnanense y Purpureocillium lilacinus, que resultaron codificar para una familia de proteasas destinadas a ejercer actividad antagonista contra un amplio rango de hospederos. Se pudo inferir que las cepas en estudio son buenos candidatos iniciales para formulación de biopreparados destinados al control biológico de organismos plaga en cultivos de interés agrícola en la región, debido a que se encontraron rasgos y genes que permitirán ejercer una amplia acción antagónica.11 páginasapplication/pdfspaIngeniería Y CompetitividadColombiaRevista Ingeniería Y Competitividad ISSN: 2027-8284, 2021 vol:23 fasc: N/A págs: 1 - 11, DOI:10.25100/iyc.23i2.11154vol.23 no.2 (2021)112 (2021)123López-Barrera GL. Análisis molecular de hongos antagonistas aislados de plantaciones de cacao (Theobroma cacao) de Norte de Santander. INGENIERÍA Y COMPETITIVIDAD, 23(2), In press 2021. https://doi.org/10.25100/iyc.v23i2.11154.López-Barrera GL. Molecular analysis of antagonist fungi isolated from plantations of cocoa (Theobroma cacao) from north of Santander. INGENIERÍA Y COMPETITIVIDAD, 23(2), In press 2021. https://doi.org/10.25100/iyc.v23i2.11154.Revista Ingeniería Y CompetitividadEste trabajo está licenciado bajo una Licencia Internacional Creative Commons Reconocimiento–NoComercial–CompartirIgual 4.0https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://revistaingenieria.univalle.edu.co/index.php/ingenieria_y_competitividad/article/view/11154Molecular analysis of antagonist fungi isolated from plantations of cocoa (Theobroma cacao) from north of SantanderAnálisis molecular de hongos antagonistas aislados de plantaciones de cacao (Theobroma cacao) de Norte de SantanderArtículo de revistahttp://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85AntagonistBiotechnologyGenetic diversityPCRRAPDAntagonistaBiotecnologíaDiversidad genéticaPCRRAPDFEDECACAO. Economía Nacional [Internet]. 2019. Available from: https://www.fedecacao.com.co/portal/inde x.php/es/2015-02-12-17-20-59/nacionales.Evans HC. Cacao diseases - the trilogy revisited. Phytopathology. 2007;97(12):1640-1643. https://doi.org/10.1094/PHYTO-97-12- 1640.Alves S. Hongos entomopatógenos. In: Alves S, editor. Control microbiano de insectos. Piracicaba: FEALQ; 1998. p. 289–381.Suarez L, Rangel A. Aislamiento de microorganismos para control biológico de Moniliophthora roreri. Acta Agronómica. 2013;62(4):370–378. Available from: https://revistas.unal.edu.co/index.php/acta _agronomica/article/view/36211.Suarez L, Cabrales CP. Identificación de especies de cepas nativas de Trichoderma sp., y Bacillus sp., y evaluación de su potencial antagonista in vitro frente al hongo patógeno nativo Moniliophthora roreri de la región nororiental en el departamento Norte de Santander. Respuestas. 2008;13(1):45-56.Suarez L. Aislamiento e identificación de Moniliophthora roreri causante de la moniliasis en municipios del nororiente colombiano y ensayos preliminares para su control biológico. Respuestas. 2006;11(1):3-9.Zambrano-Bullones K, Dávila M, Castillo M. Detección de fragmentos de ADN de hongos y su posible relación con la síntesis de proteínas de actividad entomopatógena. Revista de la Facultad de Agronomía. 2002;19(3):185-193.Súarez-contreras LY. Extracción y purificación del ADN de Moniliophthora roreri hongo que ataca el cacao, en norte de Santander. Respuestas. 2016;10(2):4-8Dávila, Martha, Zambrano, Karla, Castillo, Miguel A., Uso de la técnica RAPD para la identificación de fragmentos de ADN posiblemente relacionados con virulencia en hongos entomopatógenos. Bioagro [Internet]. 2001;13(3):93-98.Rosas-García NM. Avalos-de-León O, Villegas-Mendoza JM, Mireles-Martínez M, E, Barboza-Corona JE, et. al. Correlation between pr1 and pr2 Gene Content and Virulence in Metarhizium anisopliae Strains. Journal of Microbiology and Biotechnology. 2014;24(11): 1495-1502. https://doi.org/10.4014/jmb.1404.04044.Avalos-de León O. Identificación y análisis de los genes productores de proteasas tipo subtilisina y tipo tripsina en el hongo Metarhizium anisopliae, como factores de virulencia para su aplicación en el control biológico de insectos plaga [Master’s Thesis]. Reynosa: Centro de Biotecnología genómica. 2011.Sambrook J, Russell DW. Molecular Cloning A Laboratory Manual. 3rd ed. New York: CSHL Press; 2001. 2344 p.Vega-Contreras NA, Galvis-Serrano NF, Salazar-Mercado SA. Relaciones evolutivas de los peces Prochilodus reticulatus y Prochilodus magdalenae (Characiformes: Prochilodontidae). Revista de ciencias. 2017;21(1):163-173. https://doi.org/10.25100/rc.v21i1.6348.Gwanama C, Labuschagne M, Botha AM. Analysis of genetic variation in Cucurbita moschata by random amplified. Euphytica. 2000;113:19–24. https://doi.org/10.1023/A:1003936019095Latha J, Mukherjee P. PCR fingerprinting of some Trichoderma isolates from two Indian type culture collections a need for re-identification of these economically important fungi. Current Science. 2002;83(4):372-375. Available from: https://www.jstor.org/stable/24106834.Góes L, Lima-Da-Costa A, López-DeCarvalho-Freire L, Tinti-De-Oliveira N. Randomly Amplified Polymorphic DNA of Trichoderma isolates and antagonism against Rhizoctonia solani. Brazilian Archives of Biology and Technology. 2002;45(2):151-160. https://doi.org/10.1590/S1516- 89132002000200005.Bagga S, Hu G, Screen S, Leger RJ. Reconstructing the diversification of subtilisins in the pathogenic fungus Metarhizium anisopliae. Gene. 2004;324:159-169. https://doi.org/10.1016/j.gene.2003.09.031Hernandez-Lauzardo AN, Bautista-Baños S, Velázquez-del Valle G, HernándezRodríguez A. Uso de Microorganismos Antagonistas en el Control de Enfermedades Postcosecha en Frutos. Revista mexicana de patologia. 2007;25(1):66-74.Cano MA. Interacción De Microorganismos Benéficos En Plantas: Micorrizas, Trichoderma spp. y Pseudomonas spp. Revista U.D.C.A Actualidad & Divulgación Científica. 2011;14(2):15-31. https://doi.org/10.31910/rudca.v14.n2.201 1.771.Fernández-Larrea OV. Microorganismos antagonistas para el control Fitosanitario. Avances en el Fomento de Productos Fitosanitarios No-sintéticos. 2001;62:96- 100.Freimoser FM, Screen S, Bagga S, Hu G, Leger RJ. Expressed sequence tag (EST) analysis of two subspecies of Metarhizium anisopliae reveals a plethora of secreted proteins with potential activity in insect host. Microbiology. 2003;149(Pt 1):239- 47. https://doi.org/10.1099/mic.0.25761-0Charnley A. Fungal pathogens of insects: cuticle degrading enzymes and toxins. Advances in Botanical. 2003;40:241-321. https://doi.org/10.1016/S0065- 2296(05)40006-3.ORIGINALAnálisis molecular de hongos antagonistas aislados de plantaciones de cacao (Theobroma cacao) de Norte de Santander.pdfAnálisis molecular de hongos antagonistas aislados de plantaciones de cacao (Theobroma cacao) de Norte de Santander.pdfapplication/pdf401077https://repositorio.ufps.edu.co/bitstream/ufps/302/1/An%c3%a1lisis%20molecular%20de%20hongos%20antagonistas%20aislados%20de%20plantaciones%20de%20cacao%20%28Theobroma%20cacao%29%20de%20Norte%20de%20Santander.pdf68a9b2d8ec3fa1241fb3b15a244c9d4fMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-814828https://repositorio.ufps.edu.co/bitstream/ufps/302/2/license.txt2f9959eaf5b71fae44bbf9ec84150c7aMD52open accessTEXTAnálisis molecular de hongos antagonistas aislados de plantaciones de cacao (Theobroma cacao) de Norte de Santander.pdf.txtAnálisis molecular de hongos antagonistas aislados de plantaciones de cacao (Theobroma 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 incorporada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

7. Término.

a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.

b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.

8. Varios.

a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.

b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.

c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.

d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.
0000-0002-2328-8006332ac6b52127580d0633e118d7f54cb8600 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