Development of a Selective Method for Metabolites Extraction from Microalgae Biomass
Background: The production of biofuel and high value products from microalgae exhibits difficulties that have been widely studied to develop viable, efficient and economic methods for recovering metabolites. Objectives: This work is focused on evaluating experimental methods to obtain carbohydrates,...
- Autores:
-
Barajas Solano, andres F
González-Delgado, Angel Darío
Urbina-Suarez, Nestor Andres
Barajas, Crisóstomo
Sanguino, Paola Andrea
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad Francisco de Paula Santander
- Repositorio:
- Repositorio Digital UFPS
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.ufps.edu.co:ufps/693
- Acceso en línea:
- http://repositorio.ufps.edu.co/handle/ufps/693
- Palabra clave:
- Carbohydrates
Flocculation
Lipids
Microalgae
Proteins
- Rights
- openAccess
- License
- This work is licensed under a Creative Commons Attribution 4.0 International License.
| Summary: | Background: The production of biofuel and high value products from microalgae exhibits difficulties that have been widely studied to develop viable, efficient and economic methods for recovering metabolites. Objectives: This work is focused on evaluating experimental methods to obtain carbohydrates, proteins and lipids by varying process variables (solvent concentration, temperature, biomass/solvent ratio and moisture content). Methods/Analysis: Carbohydrate and proteins were extracted by acid and alkaline hydrolysis to study the effect of biomass moisture on recovery of these metabolites. Lipids were obtained using hexane and methanol-chloroform methods and its quantification was performed by gravimetric analysis. Findings: It was found that 41.96% and 49.77% of carbohydrates were recovered from C. vulgaris using biomass without thermal pretreatment by acid and alkaline hydrolysis, respectively. Regarding to lipid extraction, hexane was used as solvent for recovering 18.22% of lipids from C. vulgaris. In addition, results suggested that dehydrating biomass at 105°C reduces recovery of high value products. Novelty/Improvement: This study proposes a selective method for extracting metabolites, which enhances efficiency of recovery when is carried out under suitable conditions of biomass moisture, time and solvent volume. |
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