Evolutionary Relationships of the Fish Prochilodus reticulatus and Prochilodus magdalenae (Characiformes: Prochilodontidae)
A phylogenetic study was carried out between the species Prochilodus reticulatus (Valenciennes, 1850) and P. magdalenae (Steindachner, 1879) of great socioeconomic value in the country. Initially, the species were collected for DNA extraction and subsequent characterization by RAPD-PCR to establish...
- Autores:
-
Vega, Nelson
SALAZAR MERCADO, SEIR ANTONIO
Galvis, Fabian
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad Francisco de Paula Santander
- Repositorio:
- Repositorio Digital UFPS
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.ufps.edu.co:ufps/566
- Acceso en línea:
- http://repositorio.ufps.edu.co/handle/ufps/566
- Palabra clave:
- Prochilodus sp
RAPD
Phylogenetic analysis
Prochilodus sp
RAPD
Análisis filogenético
- Rights
- openAccess
- License
- Este es un artículo publicado en acceso abierto bajo una licencia Creative Commons
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Evolutionary Relationships of the Fish Prochilodus reticulatus and Prochilodus magdalenae (Characiformes: Prochilodontidae) |
| dc.title.spa.fl_str_mv |
Relaciones evolutivas de los peces Prochilodus reticulatus y Prochilodus magdalenae (Characiformes: Prochilodontidae) |
| title |
Evolutionary Relationships of the Fish Prochilodus reticulatus and Prochilodus magdalenae (Characiformes: Prochilodontidae) |
| spellingShingle |
Evolutionary Relationships of the Fish Prochilodus reticulatus and Prochilodus magdalenae (Characiformes: Prochilodontidae) Prochilodus sp RAPD Phylogenetic analysis Prochilodus sp RAPD Análisis filogenético |
| title_short |
Evolutionary Relationships of the Fish Prochilodus reticulatus and Prochilodus magdalenae (Characiformes: Prochilodontidae) |
| title_full |
Evolutionary Relationships of the Fish Prochilodus reticulatus and Prochilodus magdalenae (Characiformes: Prochilodontidae) |
| title_fullStr |
Evolutionary Relationships of the Fish Prochilodus reticulatus and Prochilodus magdalenae (Characiformes: Prochilodontidae) |
| title_full_unstemmed |
Evolutionary Relationships of the Fish Prochilodus reticulatus and Prochilodus magdalenae (Characiformes: Prochilodontidae) |
| title_sort |
Evolutionary Relationships of the Fish Prochilodus reticulatus and Prochilodus magdalenae (Characiformes: Prochilodontidae) |
| dc.creator.fl_str_mv |
Vega, Nelson SALAZAR MERCADO, SEIR ANTONIO Galvis, Fabian |
| dc.contributor.author.none.fl_str_mv |
Vega, Nelson SALAZAR MERCADO, SEIR ANTONIO Galvis, Fabian |
| dc.subject.proposal.eng.fl_str_mv |
Prochilodus sp RAPD Phylogenetic analysis |
| topic |
Prochilodus sp RAPD Phylogenetic analysis Prochilodus sp RAPD Análisis filogenético |
| dc.subject.proposal.spa.fl_str_mv |
Prochilodus sp RAPD Análisis filogenético |
| description |
A phylogenetic study was carried out between the species Prochilodus reticulatus (Valenciennes, 1850) and P. magdalenae (Steindachner, 1879) of great socioeconomic value in the country. Initially, the species were collected for DNA extraction and subsequent characterization by RAPD-PCR to establish their phylogenetic relationships using the Jaccard coefficient and the UPGMA method. The phylogenetic analysis of P. reticulatus and P. magdalenae showed a close genetic relationship, which suggests that the differences found between them may not be considered significant. |
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2018 |
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2018-04-05 |
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2021-11-01T16:20:21Z |
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2021-11-01T16:20:21Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
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http://repositorio.ufps.edu.co/handle/ufps/566 |
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10.25100/rc.v21i1.6348 |
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http://repositorio.ufps.edu.co/handle/ufps/566 |
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10.25100/rc.v21i1.6348 |
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spa |
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Revista De Ciencias ISSN: 0121-1935, 2017 vol:21 fasc: págs: 161 - 171, DOI:10.25100/rc.v21i1.6348 |
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Vol. 21 No. 1 (2017) |
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171 |
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1 (2017) |
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161 |
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21 |
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Vega Contreras, N. A., Salazar Mercado, S. A., & Galvis Serrano, N. F. (2017). Relaciones evolutivas de los peces Prochilodus reticulatus y Prochilodus magdalenae (Characiformes: Prochilodontidae). |
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Revista De Ciencias |
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Este es un artículo publicado en acceso abierto bajo una licencia Creative Commons |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) |
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Este es un artículo publicado en acceso abierto bajo una licencia Creative Commons Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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11 páginas |
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application/pdf |
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Revista De Ciencias |
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Colombia |
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https://revistaciencias.univalle.edu.co/index.php/revista_de_ciencias/article/view/6348 |
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Universidad Francisco de Paula Santander |
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Vega, Nelsonabd61a68a06ee0726dc4144434461981600SALAZAR MERCADO, SEIR ANTONIOd3fe7e1ec57f59c892212006893d7af1600Galvis, Fabian508e26c4055abdc301fd2290492a16d06002021-11-01T16:20:21Z2021-11-01T16:20:21Z2018-04-05http://repositorio.ufps.edu.co/handle/ufps/56610.25100/rc.v21i1.6348A phylogenetic study was carried out between the species Prochilodus reticulatus (Valenciennes, 1850) and P. magdalenae (Steindachner, 1879) of great socioeconomic value in the country. Initially, the species were collected for DNA extraction and subsequent characterization by RAPD-PCR to establish their phylogenetic relationships using the Jaccard coefficient and the UPGMA method. The phylogenetic analysis of P. reticulatus and P. magdalenae showed a close genetic relationship, which suggests that the differences found between them may not be considered significant.Se realizó un estudio filogenético entre las especies Prochilodus reticulatus (Valenciennes, 1850) y P. magdalenae (Steindachner, 1879) de gran valor socioeconómico en el país. Inicialmente, las especies fueron colectadas para la extracción del ADN y su posterior caracterización mediante RAPD-PCR para establecer sus relaciones filogenéticas empleando el coeficiente de Jaccard y el método UPGMA. El análisis filogenético de P. reticulatus y P. magdalenae mostró una estrecha relación genética, lo que sugiere que las diferencias encontradas entre ellas pueden considerarse no significativas.11 páginasapplication/pdfspaRevista De CienciasColombiaRevista De Ciencias ISSN: 0121-1935, 2017 vol:21 fasc: págs: 161 - 171, DOI:10.25100/rc.v21i1.6348Vol. 21 No. 1 (2017)1711 (2017)16121Vega Contreras, N. A., Salazar Mercado, S. A., & Galvis Serrano, N. F. (2017). Relaciones evolutivas de los peces Prochilodus reticulatus y Prochilodus magdalenae (Characiformes: Prochilodontidae).Revista De CienciasEste es un artículo publicado en acceso abierto bajo una licencia Creative Commonsinfo:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)http://purl.org/coar/access_right/c_abf2https://revistaciencias.univalle.edu.co/index.php/revista_de_ciencias/article/view/6348Evolutionary Relationships of the Fish Prochilodus reticulatus and Prochilodus magdalenae (Characiformes: Prochilodontidae)Relaciones evolutivas de los peces Prochilodus reticulatus y Prochilodus magdalenae (Characiformes: Prochilodontidae)Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Prochilodus spRAPDPhylogenetic analysisProchilodus spRAPDAnálisis filogenéticoIGAC. Atlas Básico de Colombia. 5a edición. Bogotá: Imprenta Nacional de Colombia; 2003.Castro R, Vari R. Detritivores of the South American fish family Prochilodontidae. 1st ed. Washington, D.C.: Smithsonian Books; 2004.Mojica JI, Galvis G, Sánchez-Duarte P, Castellanos C, Villa-Navarro FA. Peces del valle medio del río Magdalena, Colombia. Biota Colombiana. 2006; 7: 23-38.Jaramillo U, Jiménez L. Algunos aspectos biológicos de la población de Prochilodus magdalenae en las ciénagas de Tumaradó (Río Atrato), Colombia. Actual Biol. 2008; 30(88): 55-66.Maldonado JA, Vari RP, Usma JS. Checklist of the freshwater fishes of Colombia. Biota Colombiana. 2008; 9(2): 143-237.Mojica JI, Usma JS, Álvarez-León R, Lasso CA (Eds). Libro rojo de peces dulceacuícolas de Colombia 2012. Instituto de Investigación de Recursos Biológicos Alexander von Humboldt, Instituto de Ciencias Naturales de la Universidad Nacional de Colombia, WWF Colombia y Universidad de Manizales. Bogotá, D. C., Colombia. 2012.Valderrama M, Garzón AC, Salas F, Rangel B, Solano D, Fadul M. Monitoreo ictiológico y pesquero del embalse de Urrá. Informe final Año 2002 presentado a Urrá S.A. E.S.P. Montería, Colombia:Urrá S.A. E.S.P.; 2003.Valderrama M, Solano D. Estado de la población de bocachico, Prochilodus magdalenae (Pisces: Characiformes), y su manejo en la cuenca del río Sinú, Colombia. Dahlia. 2004; 7: 3-1.Galvis G, Mojica JI, Camargo M. Peces del Catatumbo. Ecopetrol-Oxy-ShellAsociación Cravo Norte. Bogotá D.C., Colombia: D’Vinni Edit. Ltda;1997.Schultz LP. A Further contribution to the ichthyology of Venezuela. Procceding Unites States National Museum. 1949; 95: 235-367.Shubina E, Ponomareva E, Klimov A, Klimova A, Kedrova O. Repetitive DNA sequences as an indicator of the level of genetic isolation in fish. Molecular Biology. 2015; 49(3): 358-368.Orozco-Berdugo G. Evaluación de la estructura genética de la población de bocachico Prochilodus magdalenae (characiformes, prochilodontidae) en la cuenca del río magdalena y sus principales tributarios - Colombia. Tesis de maestría. Universidad del Magdalena. 2013.Lopera-Barrero NM, Povh JA, Sirol RN, Rodriguez-Rodriguez M del P, Lima ECS, Poveda-Parra AR, et al. Genetic diversity of pacu and piapara broodstocks in restocking programs in the rivers Paraná and Paranapanema (Brazil). Semina: Ciências Agrárias. 2016; 37(4): 2365-2374.Alberti E, Fraga J, García R, Hernández E, Castillo L, de la Cuetara K, et al. Estudio del polimorfismo genético de las células de la médula ósea y del sistema nervioso central de ratas mediante la técnica de RAPD. Rev Colomb Biotecnol. 2011; 13(1): 42-51.Silva CMC, Kersanach R, Proietti MC, Cestari LF, D’Incao F, Lacerda AL, et al. Genetic structuring among silverside fish (Atherinella brasiliensis) populations from different Brazilian regions. Estuarine Coastal Shelf Science. 2016; 178: 0272-7714. Doi: http://dx.doi.org/10.1016/j.ecss.2016.06.007.Mohapatra TK, Chattopadhyay NR, Behera BK, Patra A. To evaluate genetic variation within and among the two different stocks of Catla catla in Orissa based on RAPD profiles. Aquaculture. 2013; 1-11.Bardakci FY, Skibinski D. Application of the RAPD technique in Tilapia fish species and subespecies identification. Heredity. 1994; 73:117-123.Zakiur S, Rahman MK, Islam S, Alam S. Genetic variation of wild and hatchery populations of the catla Indian major carp (Catla catla Hamilton 1822: Cypriniformes, Cyprinidae) revealed by RAPD markers. Genet Mol Biol. 2009; 32(1): 197-201. 2009.Islam M, Ahmed A, Azam MY, Alam M. Genetic analysis of three river populations of catla catla (HAMILTON) using randomly amplified polymorphic DNA markers. AsianAustralasian J Animal Sci. 2005; 18(4): 453-457.Sambrook J, Fritish EF, Maniatis T. Molecular cloning: a laboratory manual. 2nd end. New York: Cold Spring Harbor Laboratory Press; 1989.Hatanaka T, Galetti J. RAPD markers indicate the occurrence of structured populations in a migratory freshwater fish species. Genet Mol Biol. 2003; 26(1):19-25.Ramella MS, Kroth MA, Meurer S, Nuñer APO, Zaniboni-Filho E, Arisi ACM. Genetic variability in four fish species (Pimelodus maculatus, Prochilodus lineatus, Salminus brasiliensis and Steindachneridion scripta) from Uruguay River Basin. Braz Arch Biol Technol. 2006; 49: 589–598.Moreno CE. Métodos para medir la biodiversidad. M&T-manuales y tesis SEA, vol. 1. Zaragoza; 2001.Chen P-H, Salazar E, Fernández H, Castro L, Russo A, Vásquez S. Detección de polimorfismos RAPD en materiales de Musa sp. con respuesta diferencial al ataque de Xanthomonas campestris pv. musacearum. Agronomía Tropical. 2011; 61(2): 125-132.Hoque M, Huq H, Moon N. Molecular diversity analysis in potato (Solanum tuberosum L.) through RAPD markers. SAARC J Agriculture. 2013; 11(2): 95-102.Chan J, Li W, Hu X, Liu Y, Xu Q. Genetic diversity and population structure analysis of Qinghai-Tibetan plateau schizothoracine fish (Gymnocypris dobula) based on mtDNA D-loop sequences. Biochemical Systematics Ecol. 2016; 69: 152-160. http://dx.doi. org/10.1016/j.bse.2016.09.004.Orozco G, Narváez J. Genetic diversity and population structure of bocachico Prochilodus magdalenae (Pisces, Prochilodontidae) in the Magdalena River basin and its tributaries, Colombia. 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 incorporada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

7. Término.

a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.

b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.

8. Varios.

a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.

b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.

c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.

d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.
0000-0002-5013-2146abd61a68a06ee0726dc41444344619816000000-0002-3287-703Xd3fe7e1ec57f59c892212006893d7af16000000-0002-0026-4829508e26c4055abdc301fd2290492a16d0600 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