Modelling of DHA Production from S. Limacinum ouc88: fed-batch perspectives
Biotechnology and its need to improve industrial processes have shown the need for techniques that allow processes optimization. Computer simulation offers the advantage of determining production prospects without significant resources and experimentation time. That is why a kinetic study of DHA (do...
- Autores:
-
Contreras, B
Basto, D
Gelves Gelves, Elizabeth
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2021
- Institución:
- Universidad Francisco de Paula Santander
- Repositorio:
- Repositorio Digital UFPS
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.ufps.edu.co:ufps/6580
- Acceso en línea:
- https://repositorio.ufps.edu.co/handle/ufps/6580
- Palabra clave:
- Rights
- openAccess
- License
- Published under licence by IOP Publishing Ltd
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Modelling of DHA Production from S. Limacinum ouc88: fed-batch perspectives |
| title |
Modelling of DHA Production from S. Limacinum ouc88: fed-batch perspectives |
| spellingShingle |
Modelling of DHA Production from S. Limacinum ouc88: fed-batch perspectives |
| title_short |
Modelling of DHA Production from S. Limacinum ouc88: fed-batch perspectives |
| title_full |
Modelling of DHA Production from S. Limacinum ouc88: fed-batch perspectives |
| title_fullStr |
Modelling of DHA Production from S. Limacinum ouc88: fed-batch perspectives |
| title_full_unstemmed |
Modelling of DHA Production from S. Limacinum ouc88: fed-batch perspectives |
| title_sort |
Modelling of DHA Production from S. Limacinum ouc88: fed-batch perspectives |
| dc.creator.fl_str_mv |
Contreras, B Basto, D Gelves Gelves, Elizabeth |
| dc.contributor.author.none.fl_str_mv |
Contreras, B Basto, D Gelves Gelves, Elizabeth |
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Journal of Physics: Conference Series |
| description |
Biotechnology and its need to improve industrial processes have shown the need for techniques that allow processes optimization. Computer simulation offers the advantage of determining production prospects without significant resources and experimentation time. That is why a kinetic study of DHA (docosahexaenoic acid) production from Schizochytrium limacinum OUC88 was developed in this investigation. Based on the above, a mathematical approach to simulate DHA production in a fed-batch model is proposed in this work, using Matlab software. The experimental data for determining kinetic parameters were taken from previous investigations, and a simulated DHA level in the fed-batch mode of 150 g/L was reached. On the contrary, the simulated results in batch mode present only a maximum value of 30 g/L, demonstrating the effectiveness of the fed-batch implementation with perspectives on improving processes. |
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2021 |
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2021-09-12 |
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2022-11-21T22:36:24Z |
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2022-11-21T22:36:24Z |
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Artículo de revista |
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10.1088/1742-6596/2049/1/012076 |
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eng |
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Journal of Physics: Conference Series. Vol. 2049 No.012076 (2021) |
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Vol. 2049 N0.012076 (2021) |
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7 |
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012076 (2021) |
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1 |
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Vol.2049 |
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B Contreras et al 2021 J. Phys.: Conf. Ser. 2049 012076 |
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Journal of Physics: Conference Series |
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Published under licence by IOP Publishing Ltd |
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Atribución 4.0 Internacional (CC BY 4.0) |
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Contreras, Bd7b891095d3293f3d95be30243654b71Basto, D4731423c2243c70775b439c7825a03c0Gelves Gelves, Elizabeth48702452e3333c2d4cea3b4de2e5bb5f600Journal of Physics: Conference Series2022-11-21T22:36:24Z2022-11-21T22:36:24Z2021-09-12https://repositorio.ufps.edu.co/handle/ufps/658010.1088/1742-6596/2049/1/012076Biotechnology and its need to improve industrial processes have shown the need for techniques that allow processes optimization. Computer simulation offers the advantage of determining production prospects without significant resources and experimentation time. That is why a kinetic study of DHA (docosahexaenoic acid) production from Schizochytrium limacinum OUC88 was developed in this investigation. Based on the above, a mathematical approach to simulate DHA production in a fed-batch model is proposed in this work, using Matlab software. The experimental data for determining kinetic parameters were taken from previous investigations, and a simulated DHA level in the fed-batch mode of 150 g/L was reached. On the contrary, the simulated results in batch mode present only a maximum value of 30 g/L, demonstrating the effectiveness of the fed-batch implementation with perspectives on improving processes.application/pdfengJournal of Physics: Conference Series. Vol. 2049 No.012076 (2021)Vol. 2049 N0.012076 (2021)7012076 (2021)1Vol.2049B Contreras et al 2021 J. Phys.: Conf. Ser. 2049 012076Journal of Physics: Conference SeriesPublished under licence by IOP Publishing Ltdhttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessAtribución 4.0 Internacional (CC BY 4.0)http://purl.org/coar/access_right/c_abf2https://iopscience.iop.org/article/10.1088/1742-6596/2049/1/012076Modelling of DHA Production from S. Limacinum ouc88: fed-batch perspectivesArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Reino UnidoStoll B 2002 N-3 fatty acids and lipid peroxidation in breast cancer inhibition British Journal of Nutrition 87 193Ward O and Singh A 2005 Omega-3/6 fatty acids: alternative sources of production Process Biochemistry 40 3627Honda D, Yokochi T, Nakahara T, Erata M Higashihara T 1998 Schizochytrium limacinum sp. a new thraustochytrid from a mangrove area in the west Pacific Ocean. Mycology Research 102(4) 439Wu S, Yu S and Lin L 2005 Effect of culture conditions on docosahexaenoic acid production by Schizochytrium sp. S31 Process Biochemistry 40 3103Unagul P, Assantachai C, Phadungruengluij S, Suphantharika M, Tanticharoen M and Verduyn C 2007 Coconut water as a medium additive for the production of docosahexaenoic acid (C22:6 n3) by Schizochytrium mangrovei Sk-02. Bioresources Technology 98 281Zhu L, Zhang X, Ji L, Song X and Kuang C 2007 Changes of lipid content and fatty acid composition of Schizochytrium limacinum in response to different temperatures and salinities. Process Biochemistry 42 210Araque J, Niño L and Gelves G 2020 Industrial Scale Bioprocess Simulation for Ganoderma Lucidum Production using Superpro Designer Journal of Physics: Conference Series 1655 012077Caicedo Y, Suarez C and Gelves G 2020 Evaluation of preliminary plant design for Chlorella vulgaris microalgae production focused on cosmetics purposes Journal of Physics: Conference. Series 1655 012086bañez A, Rolon Y and Gelves G 2020 Evaluating Cost-Effective Culture Media for Nutraceutics Production from Microalgae Using Computer-Aided Large Scale Predictions Journal of Physics: Conference. Series 1655 012082Nieto L, Rivera C and Gelves G 202 Economic Assessment of Itaconic Acid Production from Aspergillus Terreus using Superpro Designer Journal of Physics: Conference Series 1655 012100Pacheco S, Niño L and Gelves G 2020 Recombinant Anti-Thrombin Production from Saccharomyces Cerevisiae: Large Scale Trends Based on Computational Predictions Journal of Physics: Conference Series 1655 012081Hernandez S, Niño L, Gelves L 2020 Simulating of Microbial Growth Scale-Up in a Stirred Tank Bioreactor for Aerobic Processes using Computational Fluid Dynamics Journal of Physics: Conference Series 1655 012109Song X, Zhang X, Kuang C, Zhu L, and Zhao X 2010 Batch kinetics and modeling of DHA production by S. limacinum OUC88. Food and Bioproducts Processing, 88(1) 26Liu J, Weng L, Zhang Q, Xu H and Ji L 2003 A mathematical model for gluconic acid fermentation by Aspergillus niger. Biochemical Engineering Journal 14 137ORIGINALModelling of DHA Production from S. Limacinum ouc88 fed-batch perspectives.pdfModelling of DHA Production from S. Limacinum ouc88 fed-batch perspectives.pdfapplication/pdf779635https://repositorio.ufps.edu.co/bitstream/ufps/6580/1/Modelling%20of%20DHA%20Production%20from%20S.%20Limacinum%20ouc88%20fed-batch%20perspectives.pdf90f97ea719354ee6ba72b8074dfcd482MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-814828https://repositorio.ufps.edu.co/bitstream/ufps/6580/2/license.txt2f9959eaf5b71fae44bbf9ec84150c7aMD52open accessTEXTModelling of DHA Production from S. Limacinum ouc88 fed-batch perspectives.pdf.txtModelling of DHA Production from S. 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Limacinum ouc88 fed-batch perspectives.pdf.jpgGenerated Thumbnailimage/jpeg9481https://repositorio.ufps.edu.co/bitstream/ufps/6580/3/Modelling%20of%20DHA%20Production%20from%20S.%20Limacinum%20ouc88%20fed-batch%20perspectives.pdf.jpg81cb57d51d7726162be8a5f19f1ce063MD53open accessufps/6580oai:repositorio.ufps.edu.co:ufps/65802022-11-23 03:00:40.232An error occurred on the license name.|||https://creativecommons.org/licenses/by/4.0/open accessRepositorio Universidad Francisco de Paula 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 incorporada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

7. Término.

a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.

b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.

8. Varios.

a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.

b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.

c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.

d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.
0000-0001-9081-684448702452e3333c2d4cea3b4de2e5bb5f600 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