Lipids production from Scenedesmus obliquus through carbon/nitrogen ratio optimization

Microalgae are one of the most promising sources of raw material for biofuel production and derivatives since its high yield of biomass and metabolites possess a low environmental impact. However, its implementation on large scale facilites still faces challenges such as the optimisation of lipid pr...

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Autores:
Barajas Solano, andres F
Urbina-Suarez, Nestor Andres
Rangel-Basto, Yeily Adriana
Cuéllar García, Diego J.
Muñoz Peñaloza, Yaneth A.
Tipo de recurso:
Article of journal
Fecha de publicación:
2019
Institución:
Universidad Francisco de Paula Santander
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Repositorio Digital UFPS
Idioma:
eng
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oai:repositorio.ufps.edu.co:ufps/538
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http://repositorio.ufps.edu.co/handle/ufps/538
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Atribución 4.0 Internacional (CC BY 4.0)
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dc.title.eng.fl_str_mv Lipids production from Scenedesmus obliquus through carbon/nitrogen ratio optimization
title Lipids production from Scenedesmus obliquus through carbon/nitrogen ratio optimization
spellingShingle Lipids production from Scenedesmus obliquus through carbon/nitrogen ratio optimization
title_short Lipids production from Scenedesmus obliquus through carbon/nitrogen ratio optimization
title_full Lipids production from Scenedesmus obliquus through carbon/nitrogen ratio optimization
title_fullStr Lipids production from Scenedesmus obliquus through carbon/nitrogen ratio optimization
title_full_unstemmed Lipids production from Scenedesmus obliquus through carbon/nitrogen ratio optimization
title_sort Lipids production from Scenedesmus obliquus through carbon/nitrogen ratio optimization
dc.creator.fl_str_mv Barajas Solano, andres F
Urbina-Suarez, Nestor Andres
Rangel-Basto, Yeily Adriana
Cuéllar García, Diego J.
Muñoz Peñaloza, Yaneth A.
dc.contributor.author.none.fl_str_mv Barajas Solano, andres F
Urbina-Suarez, Nestor Andres
Rangel-Basto, Yeily Adriana
Cuéllar García, Diego J.
Muñoz Peñaloza, Yaneth A.
description Microalgae are one of the most promising sources of raw material for biofuel production and derivatives since its high yield of biomass and metabolites possess a low environmental impact. However, its implementation on large scale facilites still faces challenges such as the optimisation of lipid production (due to strain capacity and environmental factors) and downstream processes (extraction and separation of the lipidic fraction). The objective of the present investigation was to determine the potential of the carbon/nitrogen ratio as a technical tool for the improvement of total lipids on Scenedesmus obliquus. The carbon/nitrogen ratio was evaluated using a non-factorial design coupled with surface response methodology with sodium bicarbonate and sodium nitrate as carbon and nitrogen source. Results showed that the optimal conditions that enhanced the lipid deposition (up to 66% w/w) were 1.5 g L-1 sodium bicarbonate and 0,125 g L-1 of sodium nitrate. Finally, the results of the fatty acid profile shown the presence of stearic acid (C18: 0) with 22.63% and elaidic acid (C18:1) with 77.38%, with the absence of fatty acids of two or more double bonds. In conclusion, the adjustment in the carbon/nitrogen ratio favours the final deposition of lipids in Scenedesmus obliquus which is emerging as a possible candidate for the production of lipids of interest for the generation of biodiesel.
publishDate 2019
dc.date.issued.none.fl_str_mv 2019
dc.date.accessioned.none.fl_str_mv 2021-10-31T15:35:00Z
dc.date.available.none.fl_str_mv 2021-10-31T15:35:00Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.relation.ispartof.none.fl_str_mv ournal of Physics: Conference Series ISSN: 1742-6588, 2019 vol:1388 fasc: págs: 1 - 7
dc.relation.citationedition.spa.fl_str_mv Vol. 1388, No. 012043 (2019)
dc.relation.citationendpage.spa.fl_str_mv 7
dc.relation.citationissue.spa.fl_str_mv 012043 (2019)
dc.relation.citationstartpage.spa.fl_str_mv 1
dc.relation.citationvolume.spa.fl_str_mv 1388
dc.relation.cites.none.fl_str_mv D J Cuéllar-García et al 2019 J. Phys.: Conf. Ser. 1388 012043
dc.relation.ispartofjournal.spa.fl_str_mv Journal of Physics: Conference Series
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dc.format.extent.spa.fl_str_mv 7 páginas
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dc.publisher.spa.fl_str_mv Journal of Physics: Conference Series
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spelling Barajas Solano, andres F7237a5ca918751f9d045f15b62fd2f1f600Urbina-Suarez, Nestor Andres3e8ce1897f5d9382bb88755e9ec9cb96600Rangel-Basto, Yeily Adriana923d93eb1855ac34c83159fa697af362600Cuéllar García, Diego J.71bca50c6e31d2fa3b48c4aadee8fccaMuñoz Peñaloza, Yaneth A.f10790f83964982f472a324c1b7742d12021-10-31T15:35:00Z2021-10-31T15:35:00Z2019http://repositorio.ufps.edu.co/handle/ufps/53810.1088/1742-6596/1388/1/012043Microalgae are one of the most promising sources of raw material for biofuel production and derivatives since its high yield of biomass and metabolites possess a low environmental impact. However, its implementation on large scale facilites still faces challenges such as the optimisation of lipid production (due to strain capacity and environmental factors) and downstream processes (extraction and separation of the lipidic fraction). The objective of the present investigation was to determine the potential of the carbon/nitrogen ratio as a technical tool for the improvement of total lipids on Scenedesmus obliquus. The carbon/nitrogen ratio was evaluated using a non-factorial design coupled with surface response methodology with sodium bicarbonate and sodium nitrate as carbon and nitrogen source. Results showed that the optimal conditions that enhanced the lipid deposition (up to 66% w/w) were 1.5 g L-1 sodium bicarbonate and 0,125 g L-1 of sodium nitrate. Finally, the results of the fatty acid profile shown the presence of stearic acid (C18: 0) with 22.63% and elaidic acid (C18:1) with 77.38%, with the absence of fatty acids of two or more double bonds. In conclusion, the adjustment in the carbon/nitrogen ratio favours the final deposition of lipids in Scenedesmus obliquus which is emerging as a possible candidate for the production of lipids of interest for the generation of biodiesel.7 páginasapplication/pdfengJournal of Physics: Conference Seriesournal of Physics: Conference Series ISSN: 1742-6588, 2019 vol:1388 fasc: págs: 1 - 7Vol. 1388, No. 012043 (2019)7012043 (2019)11388D J Cuéllar-García et al 2019 J. Phys.: Conf. Ser. 1388 012043Journal of Physics: Conference SeriesContent from this work may be used under the terms of theCreative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. Published under licence by IOP Publishing Ltdinfo:eu-repo/semantics/openAccessAtribución 4.0 Internacional (CC BY 4.0)http://purl.org/coar/access_right/c_abf2https://iopscience.iop.org/article/10.1088/1742-6596/1388/1/012043Lipids production from Scenedesmus obliquus through carbon/nitrogen ratio optimizationArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Pinedo J, García Prieto C V, D’Alessandro A A, Ibáñez R, Tonelli S, Díaz M S and Irabien Á 2016 Chem. Eng. Res. Des. 107 117Sibi G, Shetty V and Mokashi K 2016 J. Energy Inst. 89(3) 330Milano J, Ong H C, Masjuki H H, Chong W T, Lam M K, Loh P K and Vellayan V 2016 Renew. Sust. Energ. Rev. 58 180Solovchenko A, Verschoor A M, Jablonowski N D and Nedbal L 2016 Biotechnol. Adv. 34(5) 550Rai MP and Gupta S 2017 Energ. Convers. Manage. 141 85Zhu L 2015 Renew. Sust. Energ. Rev. 41 1376Doshi A, Pascoe S, Coglan L and Rainey T J 2016 Renew. Sust. Energ. Rev. 64 329Dragone G, Fernandes B, Vicente A A and Teixeira J A 2010 Third generation biofuels from microalgae A Mendez Vilas Ed. Current research, technology and education topics in applied microbiology and microbial biotechnology (Badajoz: Formatex Research Center) p 1355Kiran B, Pathak K, Kumar R and Deshmukh D 2016 Ecol. Eng. 92 73Villaseñor Camacho J, Fernández Marchante C M and Rodríguez Romero L 2018 Environ. Sci. Pollut. R. 25(29) 29279Esakkimuthu S, Krishnamurthy V, Govindarajan R and Swaminathan K 2016 Biomass Bioenerg. 88 126Fazeli Danesh A, Mooij P, Ebrahimi S, Kleerebezem R and van Loosdrecht M 2018 Biotechnol. Bioeng. 115(5) 1152Shin Y S, Choi H I, Choi J W, Lee J S, Sung Y J and Sim S J 2018 Bioresource Technol. 258 335Ghafari M, Rashidi B and Haznedaroglu B Z 2018 Biofuels 9(2) 147White D A, Pagarette A, Rooks P and Ali S T 2013 J. Appl. Phycol. 25(1) 153Pancha I, Chokshi K, Ghosh T, Paliwal C, Maurya R and Mishra S 2015 Bioresource Technol. 193 315Andersen R A, Berges J A, Harrison P J and Watanabe M M 2005 Appendix A-recipes for freshwater and seawater media Algal culturing techniques ed Andersen R A (Boston: Elsevier) p 429Hilbe J M 2007 Am. Stat. 61(1) 91Moheimani N R, Borowitzka M A, Isdepsky A and Fon Sing S 2013 Standard methods for measuring growth of algae and their composition Borowitzka, M A and Moheimani N R Ed Algae for biofuels and energy (Dordrecht; New York: Springer) p 265APHA 2017 Standard Methods for the Examination of Water and Waste Water 23rd Edn (Washington: American Public Health Association)European Commitee for Satandarization 2009 Automotive fuels. Fatty acid methyl esters (FAME) for diesel engines. Requirements and test methods, EN 14214 (Brussels: European Commitee for Satandarization)Srinivasan R, Mageswari A, Subramanian P, Suganthi C, Chaitanyakumar A, Aswini V and Gothandam KM 2018 Sci. Rep-UK 8(1) 6972Mondal M, Ghosh A, Oinam G, Tiwari ON, Gayen K and Halder G N 2017 Environ. Prog. Sustain. 36(5) 1498Guihéneuf F and Stengel D 2013 Pavlova lutheri Mar. Drugs 11(11) 4246Beuckels A, Smolders E and Muylaert K 2015 Water Res. 77 98Prasad M S V, Varma A K, Kumari P and Mondal P 2018 Environ Technol 39(5) 669Xin L, Hong-ying H, Ke G and Ying-xue S 2010 Bioresource Technol. 101(14) 5494Eustance E, Wray JT, Badvipour S and Sommerfeld M R 2016 J. Appl. Phycol. 28(3) 1459Ji X, Cheng J, Gong D, Zhao X, Qi Y, Su Y and Ma W 2018 Sci Total Environ 633 593Eida M F, Darwesh O M and Matter I A 2018 J. Ecol. Eng. 19(5) 38Anusree V, Sujitha B, Anand J and Arumugam M 2017 Indian J. Exp. Biol. 55 702Grobbelaar, J U 2012 Journal of Applied Phycology 24(3) 315AL-Mashhadani M K H, Wilkinson S J and Zimmerman W B 2015 Chem. Eng. Sci. 137 243Sniffen K D, Price J R, Sales C M and Olson M S 2017 Environ. Sci. Technol. 51(22) 13344Wang L, Li Y, Sommerfeld M and Hu Q 2013 Bioresource Technol. 129 289Li Y, Han F, Xu H, Mu J, Chen D, Feng B and Zeng H 2014 Bioresource Technol. 174 24González-Garcinuño Á, Tabernero A, Sánchez-Álvarez J M, Martin del Valle E M and Galán M A 2014 Bioresource Technol. 173 334Aslam A, Thomas-Hall S R, Manzoor M, Jabeen F, Iqbal M, uz Zaman Q, Schenk P M and Asif Tahir M 2018 J. Photoch. Photobio. B 179 126Mehta B H, Mandalia H V and Mistry A B A 2011 Review on effect of oxygenated fuel additive on the performance and emission characteristics of diesel engine National Conference on Recent Trends in Engineering & TechnologyNCRTET-2011 (Anand Gujarat) (Vallabh Vidyannagar: B.V.M. Engineering College) p 13ORIGINALLipids production from Scenedesmus obliquus through carbon nitrogen ratio optimization.pdfLipids production from Scenedesmus obliquus through carbon nitrogen ratio optimization.pdfapplication/pdf900307https://repositorio.ufps.edu.co/bitstream/ufps/538/1/Lipids%20production%20from%20Scenedesmus%20obliquus%20through%20carbon%20nitrogen%20ratio%20optimization.pdfc04e3c0ec9f50df1db1021d61916fea9MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-814828https://repositorio.ufps.edu.co/bitstream/ufps/538/2/license.txt2f9959eaf5b71fae44bbf9ec84150c7aMD52open accessTEXTLipids production from Scenedesmus obliquus through carbon nitrogen ratio optimization.pdf.txtLipids production from Scenedesmus obliquus through carbon nitrogen ratio optimization.pdf.txtExtracted 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 incorporada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GA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