Cytogenotoxic effect of propanil using the Lens culinaris Med and Allium cepa L test
Propanil can produce methemoglobinemia, hemolytic anemia, hepatotoxicity, metabolic disorder and nephrotoxicity. It also has a genotoxic effect, although it is not listed as a carcinogen and it continues to be applied excessively throughout the world. Consequently, in this study the cytogenotoxic ef...
- Autores:
-
SALAZAR MERCADO, SEIR ANTONIO
Quintero Caleño, Jesús David
Rojas, Jhan
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2020
- Institución:
- Universidad Francisco de Paula Santander
- Repositorio:
- Repositorio Digital UFPS
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.ufps.edu.co:ufps/423
- Acceso en línea:
- http://repositorio.ufps.edu.co/handle/ufps/423
- Palabra clave:
- Bioindicator
Environmental toxicity
Micronuclei
Mutagenic
Nuclear abnormalities
- Rights
- openAccess
- License
- 2020 Elsevier Ltd. All rights reserved.
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Cytogenotoxic effect of propanil using the Lens culinaris Med and Allium cepa L test |
| title |
Cytogenotoxic effect of propanil using the Lens culinaris Med and Allium cepa L test |
| spellingShingle |
Cytogenotoxic effect of propanil using the Lens culinaris Med and Allium cepa L test Bioindicator Environmental toxicity Micronuclei Mutagenic Nuclear abnormalities |
| title_short |
Cytogenotoxic effect of propanil using the Lens culinaris Med and Allium cepa L test |
| title_full |
Cytogenotoxic effect of propanil using the Lens culinaris Med and Allium cepa L test |
| title_fullStr |
Cytogenotoxic effect of propanil using the Lens culinaris Med and Allium cepa L test |
| title_full_unstemmed |
Cytogenotoxic effect of propanil using the Lens culinaris Med and Allium cepa L test |
| title_sort |
Cytogenotoxic effect of propanil using the Lens culinaris Med and Allium cepa L test |
| dc.creator.fl_str_mv |
SALAZAR MERCADO, SEIR ANTONIO Quintero Caleño, Jesús David Rojas, Jhan |
| dc.contributor.author.none.fl_str_mv |
SALAZAR MERCADO, SEIR ANTONIO Quintero Caleño, Jesús David Rojas, Jhan |
| dc.subject.proposal.eng.fl_str_mv |
Bioindicator Environmental toxicity Micronuclei Mutagenic Nuclear abnormalities |
| topic |
Bioindicator Environmental toxicity Micronuclei Mutagenic Nuclear abnormalities |
| description |
Propanil can produce methemoglobinemia, hemolytic anemia, hepatotoxicity, metabolic disorder and nephrotoxicity. It also has a genotoxic effect, although it is not listed as a carcinogen and it continues to be applied excessively throughout the world. Consequently, in this study the cytogenotoxic effect of propanil was evaluated, using apical root cells of Allium cepa and Lens culinaris. In which, L. culinaris seeds and A. cepa bulbs were subjected to 6 treatments with propanil (2, 4, 6, 8, 10 and 12 mg L1 ) and to distilled water as control treatment. Subsequently, the root growth was measured every 24 h for 3 days. Next, the mitotic index and cellular anomalies were determined. Whereby, decreased root development was observed in all treatments. Likewise, greater inhibition of mitosis was evidenced in L. culinaris compared to A. cepa. In addition, chromosomal abnormalities, such as nucleus absence, sticky chromosomes in metaphase and binucleated cells, were present in most of the treatments. Thus, the presence of micronuclei and the results of L. culinaris, indicate the high cytogenotoxicity of propanil and the feasibility of this species as bioindicator. |
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2020 |
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2020-02-12 |
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2021-10-26T16:48:33Z |
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2021-10-26T16:48:33Z |
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Artículo de revista |
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10.1016/j.chemosphere.2020.126193 |
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Chemosphere ISSN: 0045-6535, 2020 vol:249 fasc: N/A págs: 1-8 , DOI:10.1016/j.chemosphere.2020.126193 |
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S. A. Salazar Mercado, J. D. Quintero Caleño y J. P. Rojas Suárez, "Cytogenotoxic effect of propanil using the Lens culinaris Med and Allium cepa L test", Chemosphere, vol. 249, n.º 126193, pp. 1–8, febrero de 2020. Accedido el 26 de octubre de 2021. [En línea]. Disponible: https://doi.org/10.1016/j.chemosphere.2020.126193 |
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Chemosphere |
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2020 Elsevier Ltd. All rights reserved. |
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openAccess |
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8 páginas |
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Chemosphere |
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SALAZAR MERCADO, SEIR ANTONIOd3fe7e1ec57f59c892212006893d7af1600Quintero Caleño, Jesús David62f689bd005166545a0b8c5715b292e0600Rojas, Jhan9f39ba8c8ea66278f96ca45f4f4ef1472021-10-26T16:48:33Z2021-10-26T16:48:33Z2020-02-12http://repositorio.ufps.edu.co/handle/ufps/42310.1016/j.chemosphere.2020.126193Propanil can produce methemoglobinemia, hemolytic anemia, hepatotoxicity, metabolic disorder and nephrotoxicity. It also has a genotoxic effect, although it is not listed as a carcinogen and it continues to be applied excessively throughout the world. Consequently, in this study the cytogenotoxic effect of propanil was evaluated, using apical root cells of Allium cepa and Lens culinaris. In which, L. culinaris seeds and A. cepa bulbs were subjected to 6 treatments with propanil (2, 4, 6, 8, 10 and 12 mg L1 ) and to distilled water as control treatment. Subsequently, the root growth was measured every 24 h for 3 days. Next, the mitotic index and cellular anomalies were determined. Whereby, decreased root development was observed in all treatments. Likewise, greater inhibition of mitosis was evidenced in L. culinaris compared to A. cepa. In addition, chromosomal abnormalities, such as nucleus absence, sticky chromosomes in metaphase and binucleated cells, were present in most of the treatments. Thus, the presence of micronuclei and the results of L. culinaris, indicate the high cytogenotoxicity of propanil and the feasibility of this species as bioindicator.8 páginasapplication/pdfengChemosphereReino UnidoChemosphere ISSN: 0045-6535, 2020 vol:249 fasc: N/A págs: 1-8 , DOI:10.1016/j.chemosphere.2020.126193Vol. 249 (2020) 1261938126193 (2020)1249S. A. Salazar Mercado, J. D. Quintero Caleño y J. P. Rojas Suárez, "Cytogenotoxic effect of propanil using the Lens culinaris Med and Allium cepa L test", Chemosphere, vol. 249, n.º 126193, pp. 1–8, febrero de 2020. Accedido el 26 de octubre de 2021. [En línea]. Disponible: https://doi.org/10.1016/j.chemosphere.2020.126193Chemosphere2020 Elsevier Ltd. All rights reserved.info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://www.sciencedirect.com/science/article/abs/pii/S0045653520303866?via%3Dihub#!Cytogenotoxic effect of propanil using the Lens culinaris Med and Allium cepa L testArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85BioindicatorEnvironmental toxicityMicronucleiMutagenicNuclear abnormalitiesAbiles, J., Moreno-Torres, R., Moratalla, G., Casta no, J., P ~ erez, A.R., Mudarra, A., Machado, M.J., Planells, E., Perez de la Cruz, A., 2008. Efectos de la suplementacion con glutamina sobre el sistema antioxidante y la peroxidaci on lipídica en pacientes críticos con nutricion parenteral. Nutr. Hosp. 23 (4), 332e339.Alimba, C.G., Aladeyelu, A.M., Nwabisi, I.A., Bakare, A.A., 2018. Micronucleus cytome assay in the differential assessment of cytotoxicity and genotoxicity of cadmium and lead in Amietophrynus regularis. EXCLI Journal 17, 89e101. https://doi.org/ 10.17179/excli2017-887.Andrade-Vieira, L., Bernardes, P., Ferreira, M., 2018. Mutagenic effects of spent potliner and derivatives on Allium cepa L. and Lactuca sativa L.: a molecular approach. Chemosphere 208, 257e262.Arya, S.K., Mukherjee, A., 2014. Sensitivity of Allium cepa and Vicia faba towards cadmium toxicity. J. Soil Sci. Plant Nutr. 14, 447e458. https://doi.org/10.4067/ S071895162014005000035.Barnes, C.J., Lavy, T.L., Mattice, J.D., 1987. Exposure of non-applicator personnel and adjacent areas to aerially applied propanil. Bull. Environ. Contam. Toxicol. 39, 126e133.Bertan, A.S., Baumbach, F., Tonial, B., Pokrywiecki, S., Düsman, E., 2019. Assessment of phytoremediation potential of Allium cepa L. in raw sewage treatment. Braz. J. Biol. https://doi.org/10.1590/1519-6984.214278 (in press).Bortolotto, L.F.B., Barbosa, F.R., Silva, G., Bitencourt, T.A., Beleboni, R.O., Baek, S.J., et al., 2016. Cytotoxicity of transchalcone and licochalcone A against breast cancer cells is due to apoptosis induction and cell cycle arrest. Biomed. Pharmacother. 85, 425e433. https://doi.org/10.1016/j.biopha.2016.11.047.Bortolotto, L.F.B., Barbosa, F.R., Silva, G., Bitencourt, T.A., Beleboni, R.O., Baek, S.J., et al., 2016. Cytotoxicity of transchalcone and licochalcone A against breast cancer cells is due to apoptosis induction and cell cycle arrest. Biomed. Pharmacother. 85, 425e433. https://doi.org/10.1016/j.biopha.2016.11.047.Camp, N.E., 2007. Methemoglobinemia. J. Emerg. Nurs. 33, 172e174.Cao, Q., Rediske, R.R., Yao, L., Xie, L., 2018. Effect of microcystins on root growth, oxidative response, and exudation of rice (Oryza sativa). Ecotoxicol. Environ. Saf. 149, 143e149. https://doi.org/10.1016/j.ecoenv.2017.11.020.Carena, L., Minella, M., Barsotti, F., Brigante, M., Milan, M., Ferrero, A., Berto, S., Minero, C., Vione, D., 2017. Phototransformation of the herbicide propanil in paddy field water. Environ. Sci. Technol. 51, 2695e2704. https://doi.org/10.1021/ acs.est.6b05053.Causil, L., Coronado, J., Vega, M., Verbel, L., 2017. Efecto citotoxico del hipoclorito de sodio (NaClO), en celulas apicales de raíces de cebolla ( Allium cepa L.). Rev. Colomb. Ciencias Hortícolas 11 (1), 97e104. https://doi.org/10.17584/ rcch.2017v11i1.5662.Cavadía, T., Roche, M.M., Romero, R., 2018. Estimacion de la genotoxicidad del río Sinú mediante un bioensayo con Allium cepa l. en Montería, Cordoba-Colombia. Bistua Revista de la Facultad de Ciencias B asicas 16 (1), 174e184. https://doi.org/ 10.24054/01204211.v1.n1.2018.2941.Chiagoziem, A., Otuechere, S.O., Abarikwu, V.I., Olateju Animashaun, A.L., Oluwafemi, E.K., 2014. Protective effect of curcumin against the liver toxicity caused by propanil in rats. International Scholarly Research Notices, p. 8. https://doi.org/10.1155/2014/853697. Article ID 853697.Cisneros, P.E., 1995. La glutation reductasa y su importancia biomedica. Rev. Cubana Invest. Biomed. 14 (1) .Correa, Martins, M., Souza, V., Souza, T., 2016. Cytotoxic, genotoxic and mutagenic ^ effects of sewage sludge on Allium cepa. Chemosphere 148, 481e486.Cvjetko, P., Milosic, A., Domijan, A.M., Vinkovic, V.I., Tolic, S., Peharec, S.P., LetofskyPapst, I., Tkalec, M., Balen, B., 2017. Toxicity of silver ions and differently coated silver nanoparticles in Allium cepa roots. Ecotoxicol. Environ. Saf. 137, 18e28. https://doi.org/10.1016/j.ecoenv.2016.11.009.Darren, R., Renate, H., Nick, B., Andrew, D., Mohamed, F., Michael, E., Peter, E., 2009. Clinical outcomes and kinetics of propanil following acute self-poisoning: a prospective case series. BMC Clin. Pharmacol. 9, 3. https://doi.org/10.1186/1472- 6904-9-3.Datta, S., Singh, J., Singh, J., Singh, S., Singh, S., 2018. Assessment of genotoxic effects of pesticide and vermicompost treated soil with Allium cepa test. Sustain Environ Res 28 (4), 171e178. https://doi.org/10.1016/j.serj.2018.01.005.Dawson, A., Eddleston, M., Senarathna, L., et al., 2010. Acute human lethal toxicity of agricultural pesticides: a prospective cohort study. PLoS Med. 7.de Souza, R., de Souza, C., Bueno, O., Fontanetti, S., 2017. Genotoxicity evaluation of two metallic-insecticides using Allium cepa and Tradescantia pallida: a new alternative against leaf-cutting ants. Chemosphere 168, 1093e1099. https:// doi.org/10.1016/j.chemosphere.2016.10.098.Devine, M., Duke, S., Fedtke, C., 1993. Physiology of Herbicide Action. Prentice Hall, NJ.Disner, G.R., Rocha, M.V., Miranda, G.B., 2011. Avaliaçao da atividade mutag ~ enica do ^ Roundup® em Astyanax altiparanae (Chordata, Actinopterygii). Evidencia: biotecnologia e alimentos. 11 (1), 33e42.European Food Safety Authority (Efsa), 2011. Conclusion on the peer review of the pesticide risk assessment of the active substance propanil. EFSA Journal 9 (3), 1e63. https://doi.org/10.2903/jefsa.2011.2085.Fatma, F., Verma, S., Kamal, A., Srivastava, A., 2018. Monitoring of morphotoxic, cytotoxic and genotoxic potential of mancozeb using Allium assay. Chemosphere 195, 864e870. https://doi.org/10.1016/j.chemosphere.2017.12.052.Fendrych, M., Akhmanova, M., Merrin, J., Glanc, M., Hagihara, S., Takahashi, K., Uchida, N., Torii, K.U., Friml, J., 2018. Rapid and reversible root growth inhibition by TIR1 auxin signalling. Native Plants 4 (7), 453e459. https://doi.org/10.1038/ s41477-018-0190-1.Firbas, P., Amon, T., 2014. Chromosome damage studies in the onion plant Allium cepa L. Caryologia 67, 25-35.García, M., García, G.G.R., Hernandez, J., Pieters, 2019. Dano oxidativo y comportamiento antioxidante de ascorbato y glutation en dos genotipos de cebolla con distinta sensibilidad ante la salinidad. Bioagro 31 (2), 81-90.Ghosh, M., Bhadra, S., Adegoke, A., Bandyopadhyay, M., Mukherjee, A., 2015. MWCNT uptake in Allium cepa root cells induces cytotoxic and genotoxic responses and results in DNA hyper-methylation. Mutat. Res. 774, 49e58. https:// doi.org/10.1016/j.mrfmmm.2015.03.004.Gomes, J., Tamara, J., Moreira, V., et al., 2014. Induction of cytotoxic and genotoxic effects of guandu river waters in the Allium cepa system. Revista Ambiente & Agua 10 (1), 48e58. https://doi.org/10.4136/ambi-agua.1487.Grube, A.H., Donaldson, D., Kiely, T., Wu, L., 2011. Pesticides Industry Sales and Usage: 2006 and 2007 Market Estimates. Office of Pesticide Programs, U.S. Environmental Protection Agency, Washington, DC.Haq, I., Kumar, S., Raj, A., Lohani, M., Satyanarayana, G., 2017. Genotoxicity assessment of pulp and paper mill effluent before and after bacterial degradation using Allium cepa test. Chemosphere 169, 642e650. https://doi.org/10.1016/ j.chemosphere.2016.11.101.Haq, Z., Raj, A., Markandeya, 2018. Biodegradation of Azure-B dye by Serratia liquefaciens and its validation by phytotoxicity, genotoxicity and cytotoxicity studies. Chemosphere 196, 58e68. https://doi.org/10.1016/ j.chemosphere.2017.12.153.Hemachandra, C.K., Pathiratne, A., 2015. Assessing toxicity of copper, cadmium and chromium levels relevant to discharge limits of industrial effluents into inland Surface waters using common onion, Allium cepa bioassay. Bull. Environ. Contam. Toxicol. 94, 199e203. https://doi.org/10.1007/s00128-014-1373-8.Hong-Mei, Y., Huang, X., 2016. Inhibition of root meristem growth by cadmium involves nitric oxide-mediated repression of auxin accumulation and signalling in Arabidopsis. Plant Cell Environ. 39, 120e135. https://doi.org/10.1111/ pce.12597.Horgan, F.G., Ramal, A.F., Bernal, C.C., Villegas, J.M., Stuart, A.M., Almazan, M.L.P., 2016. Applying ecological engineering for sustainable and resilient rice production systems. Procedia Food Sci. 6, 7e15. https://doi.org/10.1016/ j.profoo.2016.02.002.Hou, Y., Li, S.H., Dong, W.L., Yuan, Y., Wang, Y.C., Shen, W.J., Li, J.Q., Cui, Z.L., 2015. Community structure of a propanil-degrading consortium and the metabolic pathway of Microbacterium sp. strain T4-7. Int. Biodeterior. Biodegrad. 105, 80e89.Huda Bhuiyan, M.N., Kang, H., Kim, J.H., Kim, S., Kho, Y., Choi, K., 2019. Endocrine disruption by several aniline derivatives and related mechanisms in a human adrenal H295R cell line and adult male zebrafish. Ecotoxicol. Environ. Saf. 180, 326e332. https://doi.org/10.1016/j.ecoenv.2019.05.003.International Seed Testing Association (Ista), 2012. International Rules for Seed Testing. Seed Science and Technology. Zürich vol. 31. Suppl. ment.Kanawi, E., Van Scoy, A.R., Budd, R., Tjeerdema, R.S., 2016. Environmental fate and ecotoxicology of propanil: a review. Toxicol. Environ. Chem. 98 (7), 689e704. https://doi.org/10.1080/02772248.2015.1133816.Karaismailoglu, M.C., 2017. Assessments on the potential genotoxic effects of fipronil insecticide on Allium cepa somatic cells. Caryologia. https://doi.org/ 10.1080/00087114.2017.1371992.Khanna, N., Sharma, S., 2013. Allium cepa root chromosomal aberration assay: a review. Indian J Pharm Biol 1 (3), 105e119.Li, W.-M., Yin, D.-Q., Zhou, Y., Hu, S.-Q., Wang, L.-S., 2003. 3,4-Dichloroanilineinduced oxidative stress in liver of crucian carp (Carassius auratus). Ecotoxicol. Environ. Saf. 56 (2), 251e255. https://doi.org/10.1016/S0147-6513(02)00117-3.Li, X., Wang, L., Wang, S., Yang, Q., Zhou, Q., Huang, X., 2018. A preliminary analysis of the effects of bisphenol A on the plant root growth via changes in endogenous plant hormones. Ecotoxicol. Environ. Saf. 150, 152e158. https://doi.org/ 10.1016/j.ecoenv.2017.12.031.Liman, R., Cigerci, I.H., Akyıl, D., Eren, Y., Konuk, M., 2011. Determination of genotoxicity of fenaminosulf by Allium and comet tests. Pestic. Biochem. Physiol. 99 (1), 61-64.Marques, R., Oehmen, A., Carvalho, G., Reis, M.A., 2015. Modelling the biodegradation kinetics of the herbicide propanil and its metabolite 3,4-dichloroaniline. Environ. Sci. Pollut. Res. 22, 6687-6695.Martins, M., Ventura de Souza, V., da Silva, T., 2016. Cytotoxic, genotoxic and mutagenic effects of sewage sludge on Allium cepa. Chemosphere 148, 481e486. https://doi.org/10.1016/j.chemosphere.2016.01.071.Milan, M., Vidotto, F., Piano, S., Negre, M., Ferrero, A., 2012. Dissipation of propanil and 3,4- dichloroaniline in three different rice management systems. J. Environ. Qual. 41, 1487e1496.Mitsou, K., Koulianou, A., Lambropoulou, D., Pappas, P., Albanis, T., Lekka, M., 2006. Growth rate effects, responses of antioxidant enzymes and metabolic fate of the herbicide Propanil in the aquatic plant Lemna minor. Chemosphere 62 (2), 275e284. https://doi.org/10.1016/j.chemosphere.2005.05.026.Modlitbova, P., Po rízka, P., Novotný, K., Drbohlavova, J., Chamradov a, I., Farka, Z., Zl amalova-Gargo sov a, H., Romih, T., Kaiser, J., 2018. Short-term assessment of cadmium toxicity and uptake from different types of Cd-based Quantum Dots in the model plant Allium cepa L. Ecotoxicol. Environ. Saf. 153, 23e31. https:// doi.org/10.1016/j.ecoenv.2018.01.044.Moreira, I., Narciso-da-Rocha, C., Lopes, A.R., Carvalho, G., Lobo-da-Cunha, A., Whitman, W.B., Snauwaert, C., Vandamme, P., Manaia, C.M., Nunes, O.C., 2017. Oryzisolibacter propanilivorax gen. nov., sp. nov., a propanil-degrading bacterium. Int. J. Syst. Evol. Microbiol. 67 (10), 3752e3758. https://doi.org/10.1099/ ijsem.0.002184Noori, R., Lorestani, B., Yousefi, N., Kolahchi, N., 2012. The effect of oil pollution on Lathyrus sativus and Lens culinaris with potential of phytoremediation. Journal of Chemical Health Risks 2 (3), 17e20. https://doi.org/10.22034/ JCHR.2018.544001.Ogeleka, D.F., Okieimen, F.E., Ekpudi, F.O., Tudararo-Aherobo, L.E., 2016. Short-term phyto-toxicity consequences of a nonselective herbicide glyphosate (Roundup™) on the growth of onions (Allium cepa Linn.). Afr. J. Biotechnol. 15 (18), 740e744. https://doi.org/10.5897/AJB2014.14355.Okayi, R.G., Tachia, M.U., Ataguba, G.A., Dikwahal, S.H., 2013. Toxicity of the herbicide propanil on Oreochromis niloticus fingerlings. J. Fish. Aquat. Sci. 8, 233-237.Otuechere, C.A., Ayoade, F., Arogundade, O.J., 2019. Impact of an acylanilide herbicide propanil on biochemical indices in kidney of diabetic rats. Asian J. Biological Sci. 12, 210e216. https://doi.org/10.3923/ajbs.2019.210.216.Patino, T.C., 2010. Variaci ~ on somaclonal y selecci on in vitro con toxinas como her- ramienta en la búsqueda de resistencia a enfermedades en plantas: revision. Revista de Investigacion Agraria y Ambiental 1 (1), 7 -15.Patlolla, A.K., Berry, A., May, L.B., Tchounwou, P.B., 2012. Genotoxicity of silver nanoparticles in Vicia faba: a pilot study on the environmental monitoring of nanoparticles. Int. J. Environ. Res. Publ. Health 9 (12), 1649-1662.Pedroso, R.M., Al-Khatib, K., Alarcon-Reverte, R., Fischer, A.J., 2016. A psbA mutation (Val219 to Ile) causes resistance to propanil and increased susceptibility to bentazon in Cyperus difformis. Pest Manag. Sci. 72 (9), 1673e1680. https:// doi.org/10.1002/ps.4267.Pereira, J.L., Duarte, M.C., Gonçalves, F., 2007. Short- and long-term responses of Daphnia spp. to propanil exposures in distinct food supply scenarios. Ecotoxicol. Environ. Saf. 68 (3), 386e396. https://doi.org/10.1016/j.ecoenv.2006.10.012.Pereira, A.C., Silva, N.C., de Almeida, L.M., 2017. Potential toxicologico de Lafoensia pacari (Lythraceae) usando o sistema teste Allium cepa como bioindicador. IV Congresso de Ensino. Pesquisa e Extensao da UEG 4 .Prajitha, V., Thoppil, J., 2016. Genotoxic and antigenotoxic potential of the aqueous leaf extracts of Amaranthus spinosus Linn. using Allium cepa assay. South Afr. J. Bot. 102, 18e25. https://doi.org/10.1016/j.sajb.2015.06.018.Primel, E.G., Zanella, R., Kurz, M.H.S., Goncalves, F.F., Martins, M.L., Machado, S.L.O., Marchesan, E., 2007. Risk assessment of surface water contamination by herbicide residues: monitoring of propanil degradation in irrigated rice field waters using HPLC-UV and confirmation by GC-MS. J. Braz. Chem. Soc. 18, 585e589.Richards, S.M., McClure, G.Y., Lavy, T.L., Mattice, J.D., Keller, R.J., Gandy, J., 2001. Propanil (3,4-dichloropropionanilide) particulate concentrations within and near the residences of families living adjacent to aerially sprayed rice fields. Arch. Environ. Contam. Toxicol. 41, 112e116. https://doi.org/10.1007/ s002440010227Rocha-Santos, C., Bastos, F.F., Dantas, R.F., Hauser-Davis, R.A., Rodrigues, L.C., Cunha, B.V., Cunha, B.J., 2018. Glutathione peroxidase and glutathione Stransferase in blood and liver from a hypoxia-tolerant fish under oxygen deprivation. Ecotoxicol. Environ. Saf. 163, 604e611. https://doi.org/10.1016/ j.ecoenv.2018.06.089.Salazar, S.A., Botello, E.A.D., 2018. Viabilidad de semillas de Glycine max (l.) Utilizando la prueba de tetrazolio. RIAA 9 (2), 89e98. https://doi.org/10.22490/ 21456453.2270.Salazar, S., Maldonado, H., 2019. Evaluation of cytotoxic potential of chlorpyrifos using Lens culinaris Med as efficient bioindicator. Ecotoxicol. Environ. Saf. 183, 109528. https://doi.org/10.1016/j.ecoenv.2019.109528.Salazar, S., Quintero, J., 2020. Cytotoxic evaluation of glyphosate, using Allium cepa Las bioindicator. Sci Total Environ. https://doi.org/10.1016/j.scitotenv.2019.134452, 700.Salazar, S., Quintero, Botello, E., 2020. Optimizacion de la prueba de tetrazolio para evaluar la vialidad en semillas de Solanum lycopersicum L, 21. Cienc Tecnol Agropecuaria.Salazar-Mercado, S., Maldonado-Bayona, H., Quintero-Caleno, J., 2018. Evaluaci ~ on de la calidad fisiologica de las semillas de Linum usitatissimum L. con la prueba de tetrazolio. Av. Invest. Agropecu. 22 (3), 46e56. http://ww.ucol.mx/revaia/ portal/pdf/2018/sept/3.pdf.Salazar-Mercado, S.A., Torres-Leon, C.A., Rojas-Su arez, J.P., 2019. Cytotoxic evaluation of sodium hypochlorite, using Pisum sativum L as effective bioindicator. Ecotoxicol. Environ. Saf. 173, 71e76. https://doi.org/10.1016/ j.ecoenv.2019.02.027.Salazar, S., Maldonado, H., 2020. Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med. Sci. Total Environ. 701, 134992. https://doi.org/10.1016/j.scitotenv.2019.134992.Sancho, E., Andreau, O., Villarroel, M.J., Fern andez-Vega, C., Tecles, F., MartínezSubiela, S., Ceron, J.J., Ferrando, M.D., 2017. European eel (Anguilla anguilla) plasma biochemistry alerts about propanil stress. J. Pestic. Sci. 42 (1), 7e15. https://doi.org/10.1584/jpestics.D16-062.Schafer, R., Ognibene, T., Malfatti, M., Turteltaub, K.W., Barnett, J.B., 2018. Comparative pharmacokinetics of high and low doses of the herbicide propanil in mice. Chem. Res. Toxicol. 31 (10), 1080e1085. https://doi.org/10.1021/ acs.chemrestox.8b00151.Shihana, F., Dawson, A.H., Dobbins, T., Dissanayake, D., Buckley, N.A., 2016. A bedside test for methaemoglobinemia improved antidote use in propanil poisoning. Clin. Toxicol. 54 (7), 576e580. https://doi.org/10.1080/ 15563650.2016.1177651.Silveira, G., Lima, M., dos Reis, G., Palmieri, M., Andrade-Vieria, L., 2017. Toxic effects of environmental pollutants: comparative investigation using Allium cepa L. and Lactuca sativa L. Chemosphere 178, 359e367. https://doi.org/10.1016/ j.chemosphere.2017.03.048.Triana, V.T.M., Henao, M.L.M., Bernal, B.M.H., 2016. Toxicidad del herbicida propanil (propanil trust® 500ec) en embriones y renacuajos de tres especies de anuros. Acta Biol. Colomb. 21 (3), 627e634. https://doi.org/10.15446/abc.v21n3.54845.Ventura-Camargo, B., de Angelis, D., Marin-Morales, M., 2016. Assessment of the cytotoxic, genotoxic and mutagenic effects of the commercial black dye in Allium cepa cells before and after bacterial biodegradation treatment. Chemosphere 161, 325-332.Vergara, Q.F., Quijano-Jara, C., 2017. Efecto del extracto acuoso de Moringa oleifera S.A. Salazar Mercado et al. / Chemosphere 249 (2020) 126193 7 sobre el índice mitotico y la frecuencia de micronucleos en Allium cepa. REBIOL 37 (2), 5-13.Verma, S., Srivastava, A., 2018. Morphotoxicity and cytogenotoxicity of pendimethalin in the test plant Allium cepa L. - a biomarker based study. Chemosphere 206, 248-254.Villarroel, M.J., Sancho, E., Andreu-Moliner, E., Ferrando, M.D., 2013. Caloric content of Daphnia magna as reflect of Propanil stress during a short-term exposure and its relationship to long-term responses. Environ. Toxicol. Pharmacol. 35, 465e472. https://doi.org/10.1016/j.etap.2013.02.012.Zhang, L., Hu, Q., Hang, P., Zhou, X., Jiang, J., 2019. Characterization of an arylamidase from a newly isolated propanil-transforming strain of Ochrobactrum sp. PP-2. Ecotoxicol. Environ. 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texttext/plain49714https://repositorio.ufps.edu.co/bitstream/ufps/423/3/Cytogenotoxic%20effect%20of%20propanil%20using%20the%20Lens%20culinaris%20Med%20and%20Allium%20cepa%20L%20test.pdf.txtafcbfae096c25b4a9fcd7198ba55f897MD53metadata only accessTHUMBNAILCytogenotoxic effect of propanil using the Lens culinaris Med and Allium cepa L test.pdf.jpgCytogenotoxic effect of propanil using the Lens culinaris Med and Allium cepa L test.pdf.jpgGenerated Thumbnailimage/jpeg14457https://repositorio.ufps.edu.co/bitstream/ufps/423/4/Cytogenotoxic%20effect%20of%20propanil%20using%20the%20Lens%20culinaris%20Med%20and%20Allium%20cepa%20L%20test.pdf.jpg39cf6f4e6fe93381a19f5b4813336602MD54metadata only accessufps/423oai:repositorio.ufps.edu.co:ufps/4232022-05-23 10:39:41.33metadata only accessRepositorio Universidad Francisco de Paula 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 incorporada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

7. Término.

a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.

b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.

8. Varios.

a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.

b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.

c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.

d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.
0000-0002-3287-703Xd3fe7e1ec57f59c892212006893d7af16000000-0002-2011-263862f689bd005166545a0b8c5715b292e0600 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