Molecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila
6 p.
- Autores:
-
Rodríguez Mateus, Zully
Vera Pacheco, Katerine
Zafra, German
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/3350
- Acceso en línea:
- https://repositorio.udes.edu.co/handle/001/3350
- Palabra clave:
- Molecular Detection
Novel Lipase Genes
Lipolytic Yeast
Candida palmioleophila
- Rights
- openAccess
- License
- Derechos Reservados - The Italian Association of Chemical Engineering, 2018
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Molecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila |
| title |
Molecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila |
| spellingShingle |
Molecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila Molecular Detection Novel Lipase Genes Lipolytic Yeast Candida palmioleophila |
| title_short |
Molecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila |
| title_full |
Molecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila |
| title_fullStr |
Molecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila |
| title_full_unstemmed |
Molecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila |
| title_sort |
Molecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila |
| dc.creator.fl_str_mv |
Rodríguez Mateus, Zully Vera Pacheco, Katerine Zafra, German |
| dc.contributor.author.spa.fl_str_mv |
Rodríguez Mateus, Zully Vera Pacheco, Katerine Zafra, German |
| dc.subject.proposal.eng.fl_str_mv |
Molecular Detection Novel Lipase Genes Lipolytic Yeast Candida palmioleophila |
| topic |
Molecular Detection Novel Lipase Genes Lipolytic Yeast Candida palmioleophila |
| description |
6 p. |
| publishDate |
2018 |
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2018-05-01 |
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2019-07-10T18:54:51Z |
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2019-07-10T18:54:51Z |
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Rodriguez-Mateus Z., Vera Pacheco K., Zafra G., 2018, Molecular detection and characterization of novel lipase genes of the lipolytic yeast candida palmioleophila, Chemical Engineering Transactions, 64, 349-354 DOI: 10.3303/CET1864059 |
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10.3303/CET1864059 |
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978-88-95608-56-3 |
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2283-9216 |
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https://repositorio.udes.edu.co/handle/001/3350 |
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Rodriguez-Mateus Z., Vera Pacheco K., Zafra G., 2018, Molecular detection and characterization of novel lipase genes of the lipolytic yeast candida palmioleophila, Chemical Engineering Transactions, 64, 349-354 DOI: 10.3303/CET1864059 10.3303/CET1864059 978-88-95608-56-3 2283-9216 |
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https://repositorio.udes.edu.co/handle/001/3350 |
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eng |
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eng |
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Chemical Engineering Transactions |
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Derechos Reservados - The Italian Association of Chemical Engineering, 2018 |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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https://creativecommons.org/licenses/by-nc/4.0/ |
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openAccess |
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Rodríguez Mateus, Zully1cb19f7b-26bc-4932-b14c-83eace262a10-1Vera Pacheco, Katerine9c6999b8-ece8-42a7-9ede-3c2bafc21c4b-1Zafra, Germanc56c5a89-cc73-40a7-9874-3d51a0b5fbaf-12019-07-10T18:54:51Z2019-07-10T18:54:51Z2018-05-016 p.In this study we analyzed the genetic variability of lipase gene sequences from eight oil and grease-degrading strains of Candida palmioleophila and to relate it to their degrading ability. The genetic variability of lipase genes was analyzed by PCR-restriction fragment length polymorphism (PCR-RFLP) and low-stringency single specific primer- PCR (LSSP-PCR), in order to obtain specific DNA fingerprints from each strain, which were subsequently compared by bioinformatic programs. DNA fingerprints were contrasted to the ability of each strain to remove palm oil in liquid culture. The results showed that at least three genes encoding lipases are present in C. palmioleophila, two of them resembling the LIP2 and LIP6 genes of C. albicans. DNA fingerprints obtained by LSSP-PCR revealed differences in the sequences of C. palmioleophila lipase genes, which allowed to group the strains according to their degrading activity. C. palmioleophila strains SACL05, SACL08 and SACL11, which showed the highest removal of palm oil after 72 h (77 to 79 % removal), were grouped in a single clade in dendrograms. Similarly, strains SACL01, SACL03, SACL06 and SACL09, which showed intermediate removal activity of palm oil (54 to 76%) grouped in a different clade. This suggests the genetic variability in lipase genes is directly related to the differences found in the efficiency of degradation of oils. On the other hand, DNA fingerprints obtained by PCR-RFLP did not allow to differentiate the strains and did not generate changes in the bands patterns between the analyzed strains. In conclusion, this study reported for the first time the detection and characterization of lipase genes from the lipolytic yeast Candida palmioleophila, and their association to the degradation of oils.application/pdfRodriguez-Mateus Z., Vera Pacheco K., Zafra G., 2018, Molecular detection and characterization of novel lipase genes of the lipolytic yeast candida palmioleophila, Chemical Engineering Transactions, 64, 349-354 DOI: 10.3303/CET186405910.3303/CET1864059978-88-95608-56-32283-9216https://repositorio.udes.edu.co/handle/001/3350engChemical Engineering TransactionsDerechos Reservados - The Italian Association of Chemical Engineering, 2018info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://www.aidic.it/cet/18/64/059.pdfMolecular DetectionNovel Lipase GenesLipolytic YeastCandida palmioleophilaMolecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophilaArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85PublicationTHUMBNAILMolecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila.pdf.jpgMolecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila.pdf.jpgGenerated Thumbnailimage/jpeg1652https://repositorio.udes.edu.co/bitstreams/a302a65b-f717-4dea-906d-3d9c0c542bcb/download86feae7c588492eab5a17887579d3f6dMD54ORIGINALMolecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila.pdfMolecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila.pdfapplication/pdf249178https://repositorio.udes.edu.co/bitstreams/5ccfab01-ca49-4d27-b4a7-5ffded9334be/download4e069f3e7b36007d7d2d085d75f0fb1cMD55TEXTMolecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila.pdf.txtMolecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila.pdf.txtExtracted texttext/plain22002https://repositorio.udes.edu.co/bitstreams/a2eeb9a2-1d1c-4967-88c2-1e505425d00d/downloadb20619aefede2696446ca69b5ed3d7fbMD53LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/4a970998-19b2-41da-8d7a-ddd488a559de/download38d94cf55aa1bf2dac1a736ac45c881cMD52001/3350oai:repositorio.udes.edu.co:001/33502023-10-11 10:19:26.709https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - The Italian Association of Chemical Engineering, 2018https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |