Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7
Digital
- Autores:
-
Prada Prada, Sergio Alfonso
Flórez Castillo, Johanna-Marcela
Farfán García, Ana Elvira
Guzmán, Fanny
Hernández Peñaranda, Indira Paola
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2020
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/5663
- Acceso en línea:
- https://doi.org/10.1371/journal.pone.0229019
https://repositorio.udes.edu.co/handle/001/5663
- Palabra clave:
- Rights
- openAccess
- License
- The Editor(s) and the Author(s), 2020
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Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
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Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
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Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
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Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
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Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
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Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
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Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
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Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
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Prada Prada, Sergio Alfonso Flórez Castillo, Johanna-Marcela Farfán García, Ana Elvira Guzmán, Fanny Hernández Peñaranda, Indira Paola |
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Prada Prada, Sergio Alfonso Flórez Castillo, Johanna-Marcela Farfán García, Ana Elvira Guzmán, Fanny Hernández Peñaranda, Indira Paola |
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CLINIUDES CIBAS |
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eng |
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eng |
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12 |
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13 de febrero de 2020 |
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Prada-Prada S, Flo´rez-Castillo J, Farfa´nGarcı´a A, Guzma´n F, Herna´ndez-Peñaranda I (2020) Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7. PLoS ONE 15 (2): e0229019. https://doi.org/10.1371/journal. pone.0229019 |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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Prada Prada, Sergio Alfonsocc945093-702a-4e7e-b598-17852200db11-1Flórez Castillo, Johanna-Marcelae5b9135c-adb9-4f3f-adf9-eae7cd9e6abf-1Farfán García, Ana Elvira74472815-bd1a-4bf4-932b-0264dd209268-1Guzmán, Fanny0ea45d23-51b8-4cbd-9a49-4283f773574d-1Hernández Peñaranda, Indira Paolaebe71324-2be9-45d1-9cfa-eef589289ef3-1CLINIUDESCIBAS2021-10-06T13:12:56Z2021-10-06T13:12:56Z2020-03-13DigitalThe development of new antimicrobial peptides has become an attractive alternative to conventional antibiotics due to the increasing rates of microbial drug resistance. Ib-M corresponds to a family of cationic synthetic peptides, 20 amino acids in length, that have shown inhibitory effect against the non-pathogenic strain Escherichia coli K-12. This work evaluated the antimicrobial potential of Ib-M peptides against the pathogenic E. coli O157: H7 using a reference strain and a clinical isolate. The Ib-M peptides showed antibacterial activity against both strains of E. coli O157: H7; the minimum inhibitory concentration of Ib-M peptides ranged from 1.6 to 12.5 μM and the minimum bactericidal concentration ranged from 3.7 to 22.9 μM, being Ib-M1 and Ib-M2 the peptides that presented the highest inhibitory effect. Time-kill kinetics assay showed a reduction of the bacterial population by more than 95% after 4 hours of exposure to 1xMIC of Ib-M1. Low cytotoxicity was observed in VERO cells with 50% cytotoxic concentration in the range from 197.5 to more than 400 μM. All peptides showed a random structure in hydrophilic environments, except Ib-M1, and all of them transitioned to an α-helical structure when the hydrophobicity of the medium was increased. In conclusion, these findings support the in vitro antimicrobial effect of Ib-M peptides against the pathogenic bacteria E. coli O157: H7 and prove to be promising molecules for the development of new therapeutic alternatives.This work was supported by the National Financing Fund for Science, Technology and Innovation Francisco José de Caldas, COLCIENCIAS (financing contract RC N°778 of 2016) and the Universidad de Santander-UDES. Dr J. F. received the funding to accomplish this project. The funders had no role in study, data collection and analysis, decision to publish, or preparation of the manuscript.Ciencias básicas biomédicas, epidemiología, salud pública enfermedades infecciosas, enfermedades no transmisibles epidemiología, salud y desarrollo, discapacidad1 p.application/pdfhttps://doi.org/10.1371/journal.pone.0229019https://repositorio.udes.edu.co/handle/001/5663engPLoS ONEEstados Unidos1213 de febrero de 20201Prada-Prada S, Flo´rez-Castillo J, Farfa´nGarcı´a A, Guzma´n F, Herna´ndez-Peñaranda I (2020) Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7. PLoS ONE 15 (2): e0229019. https://doi.org/10.1371/journal. pone.0229019PLoS ONEPLoS ONEThe Editor(s) and the Author(s), 2020info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0229019Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Todas las AudienciasPublicationORIGINALAntimicrobial activity of Ib-M peptides against Escherichia coli O157 H7.pdfAntimicrobial activity of Ib-M peptides against Escherichia coli O157 H7.pdfapplication/pdf256398https://repositorio.udes.edu.co/bitstreams/1d875970-0ed6-4c63-b673-7780d64beb80/download97264fefcad492f1e16483125e6c5972MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/a77f0766-8cf9-4f08-ad60-e1a564237879/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTAntimicrobial activity of Ib-M peptides against Escherichia coli O157 H7.pdf.txtAntimicrobial activity of Ib-M peptides against Escherichia coli O157 H7.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/17a883ff-fb20-416c-9102-ba9b4a09ea76/download5dbe86c1111d64f45ba435df98fdc825MD53THUMBNAILAntimicrobial activity of Ib-M peptides against Escherichia coli O157 H7.pdf.jpgAntimicrobial activity of Ib-M peptides against Escherichia coli O157 H7.pdf.jpgGenerated Thumbnailimage/jpeg12927https://repositorio.udes.edu.co/bitstreams/0baac5dc-9873-48fd-a2c3-c543c057a034/download169e85247d93d0f976c397569f2b3a01MD54001/5663oai:repositorio.udes.edu.co:001/56632023-10-10 09:36:01.996https://creativecommons.org/licenses/by-nc/4.0/The Editor(s) and the Author(s), 2020https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |