Human predecidual stromal cells have distinctive characteristics of pericytes : Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors
9 p.
- Autores:
-
Muñoz Fernández, Raquel
De la Mata, Claudia
Prados, Alejandro
Perea, Ana
Ruiz Magaña, María José
Llorca, Tatiana
Fernández Rubio, Pablo
Blanco Muñoz, Osmany
Abadía Molina, Ana C.
Olivares, Enrique G.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/3387
- Acceso en línea:
- https://repositorio.udes.edu.co/handle/001/3387
- Palabra clave:
- Cytokines
Decidual
Decidual stromal cells
Natural killer cells
Pericytes
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Santander, 2018
| id |
RUDES2_cba632e81ec41c39b6d74000504d79b2 |
|---|---|
| oai_identifier_str |
oai:repositorio.udes.edu.co:001/3387 |
| network_acronym_str |
RUDES2 |
| network_name_str |
Repositorio Universidad de Santander |
| repository_id_str |
|
| dc.title.eng.fl_str_mv |
Human predecidual stromal cells have distinctive characteristics of pericytes : Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors |
| title |
Human predecidual stromal cells have distinctive characteristics of pericytes : Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors |
| spellingShingle |
Human predecidual stromal cells have distinctive characteristics of pericytes : Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors Cytokines Decidual Decidual stromal cells Natural killer cells Pericytes |
| title_short |
Human predecidual stromal cells have distinctive characteristics of pericytes : Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors |
| title_full |
Human predecidual stromal cells have distinctive characteristics of pericytes : Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors |
| title_fullStr |
Human predecidual stromal cells have distinctive characteristics of pericytes : Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors |
| title_full_unstemmed |
Human predecidual stromal cells have distinctive characteristics of pericytes : Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors |
| title_sort |
Human predecidual stromal cells have distinctive characteristics of pericytes : Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors |
| dc.creator.fl_str_mv |
Muñoz Fernández, Raquel De la Mata, Claudia Prados, Alejandro Perea, Ana Ruiz Magaña, María José Llorca, Tatiana Fernández Rubio, Pablo Blanco Muñoz, Osmany Abadía Molina, Ana C. Olivares, Enrique G. |
| dc.contributor.author.spa.fl_str_mv |
Muñoz Fernández, Raquel De la Mata, Claudia Prados, Alejandro Perea, Ana Ruiz Magaña, María José Llorca, Tatiana Fernández Rubio, Pablo Blanco Muñoz, Osmany Abadía Molina, Ana C. Olivares, Enrique G. |
| dc.subject.proposal.eng.fl_str_mv |
Cytokines Decidual Decidual stromal cells Natural killer cells Pericytes |
| topic |
Cytokines Decidual Decidual stromal cells Natural killer cells Pericytes |
| description |
9 p. |
| publishDate |
2018 |
| dc.date.issued.spa.fl_str_mv |
2018-01 |
| dc.date.accessioned.spa.fl_str_mv |
2019-07-15T18:47:10Z |
| dc.date.available.spa.fl_str_mv |
2019-07-15T18:47:10Z |
| dc.type.spa.fl_str_mv |
Artículo de revista |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
http://purl.org/coar/resource_type/c_6501 |
| status_str |
publishedVersion |
| dc.identifier.doi.spa.fl_str_mv |
10.1016/j.placenta.2017.11.010 |
| dc.identifier.issn.spa.fl_str_mv |
0143-4004 |
| dc.identifier.uri.spa.fl_str_mv |
https://repositorio.udes.edu.co/handle/001/3387 |
| identifier_str_mv |
10.1016/j.placenta.2017.11.010 0143-4004 |
| url |
https://repositorio.udes.edu.co/handle/001/3387 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.spa.fl_str_mv |
Placenta |
| dc.rights.spa.fl_str_mv |
Derechos Reservados - Universidad de Santander, 2018 |
| dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.creativecommons.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0/ |
| rights_invalid_str_mv |
Derechos Reservados - Universidad de Santander, 2018 Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.source.eng.fl_str_mv |
https://www.sciencedirect.com/science/article/abs/pii/S0143400417312018?via%3Dihub |
| institution |
Universidad de Santander |
| bitstream.url.fl_str_mv |
https://repositorio.udes.edu.co/bitstreams/c9b09f1d-e091-41ba-8457-d67444be0af6/download https://repositorio.udes.edu.co/bitstreams/9237cef4-23d7-47bc-afd3-f7bb7d5bfd39/download https://repositorio.udes.edu.co/bitstreams/07c17472-dc8a-4d0e-88ec-587f54d94c3c/download https://repositorio.udes.edu.co/bitstreams/929bd05a-af16-4ac3-ae81-bde33d78f032/download https://repositorio.udes.edu.co/bitstreams/d622f2b0-6b83-410e-b26f-df03069bd531/download https://repositorio.udes.edu.co/bitstreams/fbbbf313-1662-498d-a71f-02e8355795a0/download |
| bitstream.checksum.fl_str_mv |
a375b242bbf52e15cdf1a90ee51f6bde 0d5bd228288aac742a09bfdf2c8b78ed f97717d1c27c410d8de1f73bdd9ee587 38d94cf55aa1bf2dac1a736ac45c881c cb890165efe52a0d838e52be14f03fa5 5dbe86c1111d64f45ba435df98fdc825 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Universidad de Santander |
| repository.mail.fl_str_mv |
soporte@metabiblioteca.com |
| _version_ |
1814158627592732672 |
| spelling |
Muñoz Fernández, Raquel9dcafe10-1df2-4cf5-89f3-0eb9d7764745-1De la Mata, Claudia312ade5d-9b74-4ffc-a5a2-b1fd1cb3e1ae-1Prados, Alejandro0ac381f4-6931-40ba-8aa4-0e77f5b01080-1Perea, Ana3c18b550-8e67-4188-acd8-87669919f66e-1Ruiz Magaña, María José56b194de-8116-4143-b9af-d0866be63f0f-1Llorca, Tatiana21cb9e2b-fd0a-492f-a93a-e1e62f690a26-1Fernández Rubio, Pablo52ba97ef-cdbf-4e70-a39f-f79bd96153a3-1Blanco Muñoz, Osmany1efa7cd6-62b9-4812-aee0-852e06a9b7ed-1Abadía Molina, Ana C.8bbf0f96-bafb-4cfb-bcdd-6eae3516c820-1Olivares, Enrique G.fc9240fc-e26f-4a27-88ed-cf08b5c32319-12019-07-15T18:47:10Z2019-07-15T18:47:10Z2018-019 p.Introduction Human decidual stromal cells (DSCs) play a key role in maternal–fetal interactions. Precursors of DSCs (preDSCs) localize around vessels in both the endometrium and decidua. Previous studies suggested a relationship between preDSCs and pericytes because these cells share a perivascular location, alpha smooth muscle actin (α-SM actin) expression and the ability to contract under the effects of cytokines. Methods To further study this relationship, we established 15 human preDSC lines and 3 preDSC clones. The preDSC lines and clones were tested by flow cytometry with a panel of 29 monoclonal antibodies, 14 of which are pericyte markers. The expression of angiogenic factors was determined by RT-PCR, chemotactic activity was studied with the migration assay, and cell contractility was evaluated with the collagen cell contraction assay. Confocal microscopy was used to study decidual sections. Results Under the effect of progesterone and cAMP, these lines decidualized in vitro: the cells became rounder and secreted prolactin, a marker of physiological DSC differentiation (decidualization). The antigen phenotype of these preDSC lines and clones was fully compatible with that reported for pericytes. PreDSC lines displayed pericyte characteristics: they expressed angiogenic factors and showed chemotactic and cytokine-induced contractile activity. Confocal microscopic examination of decidual sections revealed the expression of antigens detected in preDSC lines: α-SM actin colocalized with CD146, CD140b, MFG-E8, nestin, and STRO-1 (all of which are pericyte markers) in cells located around the vessels, a distinctive location of preDSCs and pericytes. Discussion Taken together, our results show that preDSCs are pericyte-like cells.application/pdf10.1016/j.placenta.2017.11.0100143-4004https://repositorio.udes.edu.co/handle/001/3387engPlacentaDerechos Reservados - Universidad de Santander, 2018info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://www.sciencedirect.com/science/article/abs/pii/S0143400417312018?via%3DihubCytokinesDecidualDecidual stromal cellsNatural killer cellsPericytesHuman predecidual stromal cells have distinctive characteristics of pericytes : Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factorsArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85PublicationBRANDED_PREVIEWHuman predecidual stromal cells have distinctive characteristics of pericytes. Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors.PNG.preview.jpgHuman predecidual stromal cells have distinctive characteristics of pericytes. Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors.PNG.preview.jpgGenerated Branded Previewimage/jpeg6073https://repositorio.udes.edu.co/bitstreams/c9b09f1d-e091-41ba-8457-d67444be0af6/downloada375b242bbf52e15cdf1a90ee51f6bdeMD54THUMBNAILHuman predecidual stromal cells have distinctive characteristics of pericytes. Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors.PNG.jpgHuman predecidual stromal cells have distinctive characteristics of pericytes. Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors.PNG.jpgUDESimage/jpeg1590https://repositorio.udes.edu.co/bitstreams/9237cef4-23d7-47bc-afd3-f7bb7d5bfd39/download0d5bd228288aac742a09bfdf2c8b78edMD53Human predecidual stromal cells have distinctive characteristics of pericytes. Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors.pdf.jpgHuman predecidual stromal cells have distinctive characteristics of pericytes. Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors.pdf.jpgGenerated Thumbnailimage/jpeg6042https://repositorio.udes.edu.co/bitstreams/07c17472-dc8a-4d0e-88ec-587f54d94c3c/downloadf97717d1c27c410d8de1f73bdd9ee587MD57LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/929bd05a-af16-4ac3-ae81-bde33d78f032/download38d94cf55aa1bf2dac1a736ac45c881cMD52ORIGINALHuman predecidual stromal cells have distinctive characteristics of pericytes. Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors.pdfHuman predecidual stromal cells have distinctive characteristics of pericytes. Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors.pdfapplication/pdf235405https://repositorio.udes.edu.co/bitstreams/d622f2b0-6b83-410e-b26f-df03069bd531/downloadcb890165efe52a0d838e52be14f03fa5MD55TEXTHuman predecidual stromal cells have distinctive characteristics of pericytes. Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors.pdf.txtHuman predecidual stromal cells have distinctive characteristics of pericytes. Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/fbbbf313-1662-498d-a71f-02e8355795a0/download5dbe86c1111d64f45ba435df98fdc825MD56001/3387oai:repositorio.udes.edu.co:001/33872023-10-11 11:09:16.227https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Santander, 2018https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |