Immobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7
Digital
- Autores:
-
Ropero Vega, José Luis
Ardila Rosas, N.
Hernández Peñaranda, Indira Paola
Flórez Castillo, Johanna-Marcela
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2020
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/5664
- Acceso en línea:
- https://doi.org/10.1016/j.apsusc.2020.146045
https://repositorio.udes.edu.co/handle/001/5664
- Palabra clave:
- Chitosan
Superparamagnetic properties
Bioconjugate
Minimum inhibitory concentration
Growth kinetics
- Rights
- closedAccess
- License
- The Editor(s) and the Author(s), 2020
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Immobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7 |
| title |
Immobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7 |
| spellingShingle |
Immobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7 Chitosan Superparamagnetic properties Bioconjugate Minimum inhibitory concentration Growth kinetics |
| title_short |
Immobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7 |
| title_full |
Immobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7 |
| title_fullStr |
Immobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7 |
| title_full_unstemmed |
Immobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7 |
| title_sort |
Immobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7 |
| dc.creator.fl_str_mv |
Ropero Vega, José Luis Ardila Rosas, N. Hernández Peñaranda, Indira Paola Flórez Castillo, Johanna-Marcela |
| dc.contributor.author.none.fl_str_mv |
Ropero Vega, José Luis Ardila Rosas, N. Hernández Peñaranda, Indira Paola Flórez Castillo, Johanna-Marcela |
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CLINIUDES CIBAS |
| dc.subject.proposal.eng.fl_str_mv |
Chitosan Superparamagnetic properties Bioconjugate Minimum inhibitory concentration Growth kinetics |
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Chitosan Superparamagnetic properties Bioconjugate Minimum inhibitory concentration Growth kinetics |
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Digital |
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2020 |
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2020-03-16 |
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2021-10-06T14:46:51Z |
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2021-10-06T14:46:51Z |
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https://doi.org/10.1016/j.apsusc.2020.146045 |
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https://repositorio.udes.edu.co/handle/001/5664 |
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https://doi.org/10.1016/j.apsusc.2020.146045 https://repositorio.udes.edu.co/handle/001/5664 |
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eng |
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eng |
| dc.relation.citationissue.spa.fl_str_mv |
15 de junio 2020 |
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515 |
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J.L. Ropero-Vega, N. Ardila-Rosas, Indira Paola Hernández, J.M. Flórez-Castillo, Immobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7, Applied Surface Science, Volume 515, 2020, 146045, ISSN 0169-4332, https://doi.org/10.1016/j.apsusc.2020.146045. |
| dc.relation.indexed.spa.fl_str_mv |
Applied Surface Science |
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Applied Surface Science |
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The Editor(s) and the Author(s), 2020 |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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https://creativecommons.org/licenses/by-nc/4.0/ |
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Applied Surface Science |
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Holanda |
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https://www.sciencedirect.com/science/article/abs/pii/S0169433220308011?via%3Dihub |
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Ropero Vega, José Luis59baaeed-2919-48eb-8b0e-06a9f627863d-1Ardila Rosas, N.ecb2c34a-d99a-4ecd-a2b5-2676bda389f9-1Hernández Peñaranda, Indira Paolaebe71324-2be9-45d1-9cfa-eef589289ef3-1Flórez Castillo, Johanna-Marcela7205eb3a-fbf2-4482-a4b0-79151dcda061-1CLINIUDESCIBAS2021-10-06T14:46:51Z2021-10-06T14:46:51Z2020-03-16DigitalAntimicrobial peptides arise as a very promising alternative for the treatment of infections generated by pathogenic microorganisms. The Ib-M2 antimicrobial peptide turns out to be a very promising candidate for these types of applications. However, it is required to evaluate immobilization systems that give peptides greater stability and activity in order to be used in biological systems. Given the above, this paper reports the preparation of superparamagnetic iron oxide nanoparticles (SPIONs) coated with chitosan in order to immobilize the Ib-M2 antimicrobial peptide. Structural properties of SPIONs were studied by DLS, SEM, XRD, FT-IR, XPS and the magnetic properties were evaluated by vibrating-sample magnetometer technique. The antimicrobial activity of the Ib-M2/SPIONs@Chi bioconjugate obtained was evaluated against Escherichia coli O157:H7 by determining the minimum inhibitory concentration (MIC). Results show that the SPIONs obtained have particle size between 10 and 15 nm with a magnetite-type structure which was confirmed by FT-IR and XPS. Characterization of magnetic properties evidences a superparamagnetic behavior of nanoparticles obtained. MIC results showed that Ib-M2/SPIONs@Chi bioconjugate exhibit a comparable or higher activity against E. coli in comparison with the free Ib-M2 peptide. These results show that the bioconjugate obtained can be considered for use in biomedical applications.Ciencias básicas biomédicas, epidemiología, salud pública enfermedades infecciosas, enfermedades no transmisibles epidemiología, salud y desarrollo, discapacidadCiencias Básicas y Aplicadas para la Sostenibilidad1 p.application/pdfhttps://doi.org/10.1016/j.apsusc.2020.146045https://repositorio.udes.edu.co/handle/001/5664engApplied Surface ScienceHolanda15 de junio 2020515J.L. Ropero-Vega, N. Ardila-Rosas, Indira Paola Hernández, J.M. Flórez-Castillo, Immobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7, Applied Surface Science, Volume 515, 2020, 146045, ISSN 0169-4332, https://doi.org/10.1016/j.apsusc.2020.146045.Applied Surface ScienceApplied Surface ScienceThe Editor(s) and the Author(s), 2020info:eu-repo/semantics/closedAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_14cbhttps://www.sciencedirect.com/science/article/abs/pii/S0169433220308011?via%3DihubChitosanSuperparamagnetic propertiesBioconjugateMinimum inhibitory concentrationGrowth kineticsImmobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Todas las AudienciasPublicationORIGINALImmobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157.H7.pdfImmobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157.H7.pdfapplication/pdf235938https://repositorio.udes.edu.co/bitstreams/fd8d74d5-228e-4eac-91b6-36b04b0f72d7/download7fafc2bf1eb4ee7744fa47cca962a1d7MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/9dd2cf06-6a25-4683-9cb1-e2a15a66923d/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTImmobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157.H7.pdf.txtImmobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157.H7.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/a7dbe38a-d956-4847-ac83-43a5fe4d7ced/download5dbe86c1111d64f45ba435df98fdc825MD53THUMBNAILImmobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157.H7.pdf.jpgImmobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157.H7.pdf.jpgGenerated Thumbnailimage/jpeg5682https://repositorio.udes.edu.co/bitstreams/19a25730-1ef4-4ec3-a8e9-fed964cbb51b/download44fbe64576f74eaebb4c98f17096c231MD54001/5664oai:repositorio.udes.edu.co:001/56642023-10-10 09:35:43.707https://creativecommons.org/licenses/by-nc/4.0/The Editor(s) and the Author(s), 2020https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |