Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (Citral, limonene and caryophyllene oxide)

16 p.

Autores:: Moreno Moreno, Erika Marcela
Leal Pinto, Sandra Milena
Stashenko, Elena E.
García Sánchez, Liliana Torcoroma

Tipo de recurso:: Article of journal

Fecha de publicación:: 2018

Institución:: Universidad de Santander

Repositorio:: Repositorio Universidad de Santander

Idioma:: eng

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dc.title.spa.fl_str_mv	Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (Citral, limonene and caryophyllene oxide)
title	Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (Citral, limonene and caryophyllene oxide)
spellingShingle	Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (Citral, limonene and caryophyllene oxide) Lippia alba Essential oils Citral Caryophyllene oxide Limonene Synergy Trypanosoma cruzi
title_short	Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (Citral, limonene and caryophyllene oxide)
title_full	Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (Citral, limonene and caryophyllene oxide)
title_fullStr	Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (Citral, limonene and caryophyllene oxide)
title_full_unstemmed	Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (Citral, limonene and caryophyllene oxide)
title_sort	Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (Citral, limonene and caryophyllene oxide)
dc.creator.fl_str_mv	Moreno Moreno, Erika Marcela Leal Pinto, Sandra Milena Stashenko, Elena E. García Sánchez, Liliana Torcoroma
dc.contributor.author.spa.fl_str_mv	Moreno Moreno, Erika Marcela Leal Pinto, Sandra Milena Stashenko, Elena E. García Sánchez, Liliana Torcoroma
dc.subject.proposal.spa.fl_str_mv	Lippia alba Essential oils Citral Caryophyllene oxide Limonene Synergy Trypanosoma cruzi
topic	Lippia alba Essential oils Citral Caryophyllene oxide Limonene Synergy Trypanosoma cruzi
description	16 p.
publishDate	2018
dc.date.issued.spa.fl_str_mv	2018-07-27
dc.date.accessioned.spa.fl_str_mv	2019-06-20T14:01:48Z
dc.date.available.spa.fl_str_mv	2019-06-20T14:01:48Z
dc.type.spa.fl_str_mv	Artículo de revista
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dc.identifier.doi.spa.fl_str_mv	10.1186/s12906-018-2293-7
dc.identifier.issn.spa.fl_str_mv	1472-6882
dc.identifier.uri.spa.fl_str_mv	https://repositorio.udes.edu.co/handle/001/3169
identifier_str_mv	10.1186/s12906-018-2293-7 1472-6882
url	https://repositorio.udes.edu.co/handle/001/3169
dc.language.iso.spa.fl_str_mv	eng
language	eng
dc.relation.ispartof.spa.fl_str_mv	BMC Complementary and Alternative Medicine
dc.rights.spa.fl_str_mv	Derechos Reservados - Universidad de Santander, 2018
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dc.source.spa.fl_str_mv	https://link.springer.com/content/pdf/10.1186%2Fs12906-018-2293-7.pdf
institution	Universidad de Santander
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spelling	Moreno Moreno, Erika Marcela9f50910e-4e7d-4b84-b369-774ac0f07d44-1Leal Pinto, Sandra Milena595a83be-311e-4b69-9ecd-ead999adb5af-1Stashenko, Elena E.9bce8ceb-c05b-4ad6-9b18-c456b7d82339-1García Sánchez, Liliana Torcoroma5b6d6a1a-21c5-4db6-8dc3-3f5c61c9190f-12019-06-20T14:01:48Z2019-06-20T14:01:48Z2018-07-2716 p.Background Chagas Disease caused by Trypanosoma cruzi infection, is one of the most important neglected tropical diseases (NTD), without an effective therapy for the successful parasite eradication or for the blocking of the disease’s progression, in its advanced stages. Due to their low toxicity, wide pharmacologic spectrum, and potential synergies, medicinal plants as Lippia alba, offer a promising reserve of bioactive molecules. The principal goal of this work is to characterize the inhibitory properties and cellular effects of the Citral and Carvone L. alba chemotype essential oils (EOs) and their main bioactive terpenes (and the synergies among them) on T. cruzi forms. Methods Twelve L. alba EOs, produced under diverse environmental conditions, were extracted by microwave assisted hydrodistillation, and chemically characterized using gas chromatography coupled mass spectrometry. Trypanocidal activity and cytotoxicity were determined for each oil, and their major compounds, on epimastigotes (Epi), trypomastigotes (Tryp), amastigotes (Amas), and Vero cells. Pharmacologic interactions were defined by a matrix of combinations among the most trypanocidal terpenes (limonene, carvone; citral and caryophyllene oxide). The treated cell phenotype was assessed by fluorescent and optic microscopy, flow cytometry, and DNA electrophoresis assays. Results The L. alba EOs displayed significant differences in their chemical composition and trypanocidal performance (p = 0.0001). Citral chemotype oils were more trypanocidal than Carvone EOs, with Inhibitory Concentration 50 (IC50) of 14 ± 1.5 μg/mL, 22 ± 1.4 μg/mL and 74 ± 4.4 μg/mL, on Epi, Tryp and Amas, respectively. Limonene exhibited synergistic interaction with citral, caryophyllene oxide and Benznidazole (decreasing by 17 times its IC50) and was the most effective and selective treatment. The cellular analysis suggested that these oils or their bioactive terpenes (citral, caryophyllene oxide and limonene) could be inducing T. cruzi cell death by an apoptotic-like mechanism. Conclusions EOs extracted from L. alba Citral chemotype demonstrated significant trypanocidal activity on the three forms of T. cruzi studied, and their composition and trypanocidal performance were influenced by production parameters. Citral, caryophyllene oxide, and limonene showed a possible induction of an apoptotic-like phenotype. The best selective anti-T. cruzi activity was achieved by limonene, the effects of which were also synergic with citral, caryophyllene oxide and benznidazole.application/pdf10.1186/s12906-018-2293-71472-6882https://repositorio.udes.edu.co/handle/001/3169engBMC Complementary and Alternative MedicineDerechos Reservados - Universidad de Santander, 2018info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://link.springer.com/content/pdf/10.1186%2Fs12906-018-2293-7.pdfLippia albaEssential oilsCitralCaryophyllene oxideLimoneneSynergyTrypanosoma cruziInduction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (Citral, limonene and caryophyllene oxide)Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/updatedVersionhttp://purl.org/coar/version/c_dc82b40f9837b551PublicationTHUMBNAILInduction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (citral, limonene and caryophyllene oxide).pdf.jpgInduction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (citral, limonene and caryophyllene oxide).pdf.jpgGenerated Thumbnailimage/jpeg1734https://repositorio.udes.edu.co/bitstreams/3b915114-0046-4c68-9cac-df2344129c69/download56bfc2b677d8f5b8e109ec37de17626bMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/90da0d48-35b6-4fc7-8e34-f0e3f51ec69a/download38d94cf55aa1bf2dac1a736ac45c881cMD52ORIGINALInduction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (citral, limonene and caryophyllene oxide).pdfInduction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (citral, limonene and caryophyllene oxide).pdfDocumento Principalapplication/pdf2995344https://repositorio.udes.edu.co/bitstreams/882223aa-967f-4b12-8931-db5b1e108777/download26b43554815ff7c1d2136c71d165ac85MD51TEXTInduction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (citral, limonene and caryophyllene oxide).pdf.txtInduction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (citral, limonene and caryophyllene oxide).pdf.txtExtracted texttext/plain73160https://repositorio.udes.edu.co/bitstreams/006f748f-adca-4116-ae0e-9814a87dce57/download38236a2e887af938c4c737607b9274fdMD53001/3169oai:repositorio.udes.edu.co:001/31692023-10-10 09:56:29.605https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Santander, 2018https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo=

Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (Citral, limonene and caryophyllene oxide)

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