Molecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oil contaminated soils
6 p.
- Autores:
-
Zafra, German
Agualimpia Valderrama, Bayron-Enrique
Regino, Ronald
Aguilar, Fabiola
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/3464
- Acceso en línea:
- https://repositorio.udes.edu.co/handle/001/3464
- Palabra clave:
- Oil-degrading
Bacteria
Isolated
Automotive Service Station
Oilcontaminated
Soils
- Rights
- openAccess
- License
- Derechos Reservados - The Italian Association of Chemical Engineering, 2016
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Molecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oil contaminated soils |
| title |
Molecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oil contaminated soils |
| spellingShingle |
Molecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oil contaminated soils Oil-degrading Bacteria Isolated Automotive Service Station Oilcontaminated Soils |
| title_short |
Molecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oil contaminated soils |
| title_full |
Molecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oil contaminated soils |
| title_fullStr |
Molecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oil contaminated soils |
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Molecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oil contaminated soils |
| title_sort |
Molecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oil contaminated soils |
| dc.creator.fl_str_mv |
Zafra, German Agualimpia Valderrama, Bayron-Enrique Regino, Ronald Aguilar, Fabiola |
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Zafra, German Agualimpia Valderrama, Bayron-Enrique Regino, Ronald Aguilar, Fabiola |
| dc.subject.proposal.eng.fl_str_mv |
Oil-degrading Bacteria Isolated Automotive Service Station Oilcontaminated Soils |
| topic |
Oil-degrading Bacteria Isolated Automotive Service Station Oilcontaminated Soils |
| description |
6 p. |
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2016 |
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2016-04 |
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2019-07-26T13:26:55Z |
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Zafra G., Regino R., Agualimpia B., Aguilar F., 2016, Molecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oil-contaminated soils, Chemical Engineering Transactions, 49, 511-516 DOI: 10.3303/CET1649086 |
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10.3303/CET1649086 |
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978-88-95608-40-2 |
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2283-9216 |
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https://repositorio.udes.edu.co/handle/001/3464 |
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Zafra G., Regino R., Agualimpia B., Aguilar F., 2016, Molecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oil-contaminated soils, Chemical Engineering Transactions, 49, 511-516 DOI: 10.3303/CET1649086 10.3303/CET1649086 978-88-95608-40-2 2283-9216 |
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https://repositorio.udes.edu.co/handle/001/3464 |
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eng |
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eng |
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Chemical Engineering Transactions |
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Derechos Reservados - The Italian Association of Chemical Engineering, 2016 |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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https://creativecommons.org/licenses/by-nc/4.0/ |
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Zafra, Germanc56c5a89-cc73-40a7-9874-3d51a0b5fbaf-1Agualimpia Valderrama, Bayron-Enrique28507682-b9a2-4a8d-9364-686d6c5f9207-1Regino, Ronaldf3ba095f-fda9-4b2f-96f1-286c5ab4891f-1Aguilar, Fabiola59755d52-14fc-4e6f-8fee-4303bdeea3a8-12019-07-26T13:26:55Z2019-07-26T13:26:55Z2016-046 p.In this study, a hydrocarbon-degrading mixed inoculum which is able to use used oil as sole carbon source, was selected from 15 bacterial isolates obtained from automotive service station oil-contaminated soils. Degrading microorganisms were isolated using different oils as sole carbon source and identified by the amplification and sequencing of the 16s rRNA sequences. In addition, the presence of hydrocarbon-degrading genes such as catechol 2,3 dioxygenase (nahH), alkane monooxygenase (alkB), Gram-negative (GN-RHDα) and Gram-positive PAH-Ring Hydroxylating Dioxygenase alpha (GP-RHDα) was analyzed by PCR and the molecular diversity by LSSP-PCR methods. Four (4) out of fifteen (15) isolates corresponding to Stenotrophomonas maltophilia, Pseudomonas aeruginosa and Klebsiella pneumoniae showed significant differences regarding oil/grease removal in liquid culture after 72 hours. Subsequently, a degrading mixed inoculum composed of these isolates was constructed and its degrading potential tested in a two-liter bioreactor containing unsterile liquid oily wastes with 0.8 % (w/v) Total Petroleum Hydrocarbons (TPHs) concentration for 42 days. The use of the mixed inoculum led to a decrease of 98.4 % Biological Oxygen Demand (BOD), 97.5 % Chemical Oxygen Demand (COD) and 97.2 % TPHs after 40 days. Further scale-up of the process to five liters using 0.2 % (w/v) unsterile oily wastes produced similar results, with a reduction of 85 % BOD, 39 % COD and 87 % TPHs after 38 days. The degrading mixed microbial inoculum presented high potential for the treatment of impacted soils at automotive service stations and sites polluted with oily wastes due to its elevated growth at high hydrocarbon concentrations and its capacity to utilize oils as energy source.application/pdfZafra G., Regino R., Agualimpia B., Aguilar F., 2016, Molecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oil-contaminated soils, Chemical Engineering Transactions, 49, 511-516 DOI: 10.3303/CET164908610.3303/CET1649086978-88-95608-40-22283-9216https://repositorio.udes.edu.co/handle/001/3464engChemical Engineering TransactionsDerechos Reservados - The Italian Association of Chemical Engineering, 2016info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://www.researchgate.net/publication/301543610_Molecular_Characterization_and_Evaluation_of_Oil-degrading_Native_Bacteria_Isolated_from_Automotive_Service_Station_Oil-contaminated_Soils/link/5719984c08aed43f63235726/downloadOil-degradingBacteriaIsolatedAutomotive Service StationOilcontaminatedSoilsMolecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oil contaminated soilsArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85PublicationTHUMBNAILMolecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oilcontaminated soils.pdf.jpgMolecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oilcontaminated soils.pdf.jpgGenerated Thumbnailimage/jpeg1632https://repositorio.udes.edu.co/bitstreams/e238ffad-3f34-4b39-b1a9-9f433716f8f8/downloadcc387cc9d88d3211e59f43a67b88a489MD54ORIGINALMolecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oilcontaminated soils.pdfMolecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oilcontaminated soils.pdfapplication/pdf262239https://repositorio.udes.edu.co/bitstreams/df4438f8-78de-410c-a2f0-0e690d1bdd6e/download0c375a04607bbaa30ac59e52287eaf60MD55TEXTMolecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oilcontaminated soils.pdf.txtMolecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oilcontaminated soils.pdf.txtExtracted texttext/plain23478https://repositorio.udes.edu.co/bitstreams/2f040fa0-dbcf-4da9-88a3-c1aff14811b9/downloada6b8013dd3d5d29b2c270913eaece4faMD53LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/c52b4b18-01f6-4292-96c3-ece244b034cd/download38d94cf55aa1bf2dac1a736ac45c881cMD52001/3464oai:repositorio.udes.edu.co:001/34642023-10-11 10:17:24.112https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - The Italian Association of Chemical Engineering, 2016https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |