Toxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling
14 p.
- Autores:
-
Suárez Barrera, Miguel Orlando
Florez, Alvaro M.
Morales, Gloria M.
Rivera, Karen Viviana
Orduz, Sergio
Ochoa, Rodrigo
Guerra, Diego
Muskus, Carlos
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/3157
- Acceso en línea:
- https://repositorio.udes.edu.co/handle/001/3157
- Palabra clave:
- Bacillus thuringiensis
Cry11
DNA shuffling
Docking
Aedes aegypti
Culex quinquefasciatus
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)
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Toxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling |
| title |
Toxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling |
| spellingShingle |
Toxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling Bacillus thuringiensis Cry11 DNA shuffling Docking Aedes aegypti Culex quinquefasciatus |
| title_short |
Toxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling |
| title_full |
Toxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling |
| title_fullStr |
Toxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling |
| title_full_unstemmed |
Toxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling |
| title_sort |
Toxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling |
| dc.creator.fl_str_mv |
Suárez Barrera, Miguel Orlando Florez, Alvaro M. Morales, Gloria M. Rivera, Karen Viviana Orduz, Sergio Ochoa, Rodrigo Guerra, Diego Muskus, Carlos |
| dc.contributor.author.spa.fl_str_mv |
Suárez Barrera, Miguel Orlando Florez, Alvaro M. Morales, Gloria M. Rivera, Karen Viviana Orduz, Sergio Ochoa, Rodrigo Guerra, Diego Muskus, Carlos |
| dc.subject.proposal.eng.fl_str_mv |
Bacillus thuringiensis Cry11 DNA shuffling Docking Aedes aegypti Culex quinquefasciatus |
| topic |
Bacillus thuringiensis Cry11 DNA shuffling Docking Aedes aegypti Culex quinquefasciatus |
| description |
14 p. |
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2018 |
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2018-10-17 |
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2019-06-19T19:44:43Z |
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2019-06-19T19:44:43Z |
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10.3389/fmicb.2018.02461 |
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1664-302X |
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https://repositorio.udes.edu.co/handle/001/3157 |
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eng |
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eng |
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Frontiers in Microbiology |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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https://creativecommons.org/licenses/by-nc/4.0/ |
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openAccess |
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Suárez Barrera, Miguel Orlando335becb1-2371-418a-acf8-21265141ac00-1Florez, Alvaro M.03b37924-311e-4ca6-bae7-fb60528ecb1c-1Morales, Gloria M.a93b5221-f977-40e6-8b51-7c9ebacd5ccc-1Rivera, Karen Viviana50c4cecd-a91c-4b82-b4e9-7cef19692897-1Orduz, Sergiod94079f7-48c0-4449-85b1-539f320c34c9-1Ochoa, Rodrigo2d4d4326-7431-45a2-b961-40d969f3aa55-1Guerra, Diego52faafc3-b5b8-4e97-a762-b53710a73b90-1Muskus, Carlosb011ee00-2da5-4ae8-a45c-96efa919d8d8-12019-06-19T19:44:43Z2019-06-19T19:44:43Z2018-10-1714 p.The Cry11 family belongs to a large group of d-endotoxins that share three distinct structural domains. Among the dipteran-active toxins referred to as three-domain Cry11 toxins, the Cry11Aa protein from Bacillus thuringiensis subsp. israelensis (Bti) has been the most extensively studied. Despite the potential of Bti as an effective biological control agent, the understanding of Cry11 toxins remains incomplete. In this study, five Cry11 variants obtained via DNA shuffling displayed toxic activity against Aedes aegypti and Culex quinquefasciatus. Three of these Cry11 variants (8, 23, and 79) were characterized via 3D modeling and analysis of docking with ALP1. The relevant mutations in these variants, such as deletions, insertions and point mutations, are discussed in relation to their structural domains, toxic activities and toxin-receptor interactions. Importantly, deletion of the N-terminal segment in domain I was not associated with any change in toxic activity, and domain III exhibited higher sequence variability than domains I and II. Variant 8 exhibited up to 3.78- and 6.09-fold higher toxicity to A. aegypti than Cry11Bb and Cry11Aa, respectively. Importantly, variant 79 showed an a-helix conformation at the C-terminus and formed crystals retaining toxic activity. These findings indicate that five Cry11 variants were preferentially reassembled from the cry11Aa gene during DNA shuffling. The mutations described in loop 2 and loop 3 of domain II provide valuable information regarding the activity of Cry11 toxins against A. aegypti and C. quinquefasciatus larvae and reveal new insights into the application of directed evolution strategies to study the genetic variability of specific domains in cry11 family genes.application/pdf10.3389/fmicb.2018.024611664-302Xhttps://repositorio.udes.edu.co/handle/001/3157engFrontiers in MicrobiologyDerechos Reservados - Florez, Suarez-Barrera, Morales, Rivera, Orduz, Ochoa, Guerra and Muskus, 2018info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://www.frontiersin.org/articles/10.3389/fmicb.2018.02461/fullBacillus thuringiensisCry11DNA shufflingDockingAedes aegyptiCulex quinquefasciatusToxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shufflingArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85PublicationTHUMBNAILToxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling.pdf.jpgToxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling.pdf.jpgGenerated Thumbnailimage/jpeg1684https://repositorio.udes.edu.co/bitstreams/b90911c9-1ef5-4dd8-8c2c-c93c8f5c5a83/downloadc7b81988f5fe569a03ef7658ed8bb089MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/97c9eb79-7b14-47e2-811e-d67bebf06263/download38d94cf55aa1bf2dac1a736ac45c881cMD52ORIGINALToxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling.pdfToxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling.pdfapplication/pdf290627https://repositorio.udes.edu.co/bitstreams/a0c5816d-ea43-41c9-a546-1be0c222de5d/downloadcc7b26d21982e75a4656dc238f27bb6bMD55TEXTToxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling.pdf.txtToxic activity, molecular modeling and docking simulations of Bacillus thuringiensis Cry11 toxin variants obtained via DNA shuffling.pdf.txtExtracted texttext/plain69853https://repositorio.udes.edu.co/bitstreams/699b1511-d3ed-4dc9-8ced-94568f6825d4/downloadb5b81378476524e369984fb0ea9b7f8aMD53001/3157oai:repositorio.udes.edu.co:001/31572023-10-11 11:08:21.739https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Florez, Suarez-Barrera, Morales, Rivera, Orduz, Ochoa, Guerra and Muskus, 2018https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |