Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections
12 p.
- Autores:
-
Gómez Calderón, Cecilia
Mesa Castro, Carol
Robledo, Sara
Gómez Rangel, Sergio-Yebrail
Bolivar Avila, Santiago
Diaz Castillo, Fredyc
Martínez Gutiérrez, Marlen
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2017
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/3217
- Acceso en línea:
- https://repositorio.udes.edu.co/handle/001/3217
- Palabra clave:
- Dengue Virus
Chikungunya virus
Antiviral
Mammea americana
Tabernaemontana cymosa
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Santander, 2017
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Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections |
| title |
Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections |
| spellingShingle |
Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections Dengue Virus Chikungunya virus Antiviral Mammea americana Tabernaemontana cymosa |
| title_short |
Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections |
| title_full |
Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections |
| title_fullStr |
Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections |
| title_full_unstemmed |
Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections |
| title_sort |
Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections |
| dc.creator.fl_str_mv |
Gómez Calderón, Cecilia Mesa Castro, Carol Robledo, Sara Gómez Rangel, Sergio-Yebrail Bolivar Avila, Santiago Diaz Castillo, Fredyc Martínez Gutiérrez, Marlen |
| dc.contributor.author.spa.fl_str_mv |
Gómez Calderón, Cecilia Mesa Castro, Carol Robledo, Sara Gómez Rangel, Sergio-Yebrail Bolivar Avila, Santiago Diaz Castillo, Fredyc Martínez Gutiérrez, Marlen |
| dc.subject.proposal.eng.fl_str_mv |
Dengue Virus Chikungunya virus Antiviral Mammea americana Tabernaemontana cymosa |
| topic |
Dengue Virus Chikungunya virus Antiviral Mammea americana Tabernaemontana cymosa |
| description |
12 p. |
| publishDate |
2017 |
| dc.date.issued.spa.fl_str_mv |
2017-01-18 |
| dc.date.accessioned.spa.fl_str_mv |
2019-06-26T20:02:37Z |
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2019-06-26T20:02:37Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
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Text |
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info:eu-repo/semantics/article |
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http://purl.org/redcol/resource_type/ART |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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publishedVersion |
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10.1186/s12906-017-1562-1 |
| dc.identifier.issn.spa.fl_str_mv |
1472-6882 |
| dc.identifier.uri.spa.fl_str_mv |
https://repositorio.udes.edu.co/handle/001/3217 |
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10.1186/s12906-017-1562-1 1472-6882 |
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https://repositorio.udes.edu.co/handle/001/3217 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.eng.fl_str_mv |
BMC Complementary and Alternative Medicine |
| dc.rights.spa.fl_str_mv |
Derechos Reservados - Universidad de Santander, 2017 |
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http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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https://creativecommons.org/licenses/by-nc/4.0/ |
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Derechos Reservados - Universidad de Santander, 2017 Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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https://bmccomplementalternmed.biomedcentral.com/track/pdf/10.1186/s12906-017-1562-1 |
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Universidad de Santander |
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Gómez Calderón, Cecilia67c5707f-226f-4225-9eb9-e983b3c099a2-1Mesa Castro, Carol020b1ff3-d743-44e5-af4d-5fd0c9e34bd9-1Robledo, Sara560476b3-8a1f-4d5f-801c-46a336bd255e-1Gómez Rangel, Sergio-Yebrail0347d3d7-298c-4b8a-a82b-e34275e1ea44-1Bolivar Avila, Santiago0adb6873-c9a5-47fa-ad60-b5ad5313c325-1Diaz Castillo, Fredyc7e7c0178-2236-4f50-8873-7431809d6a27-1Martínez Gutiérrez, Marlen92bafe7d-5768-4f16-a861-1d5a7486c5bc-12019-06-26T20:02:37Z2019-06-26T20:02:37Z2017-01-1812 p.Background The transmission of Dengue virus (DENV) and Chikungunya virus (CHIKV) has increased worldwide, due in part to the lack of a specific antiviral treatment. For this reason, the search for compounds with antiviral potential, either as licensed drugs or in natural products, is a research priority. The objective of this study was to identify some of the compounds that are present in Mammea americana (M. americana) and Tabernaemontana cymosa (T. cymosa) plants and, subsequently, to evaluate their cytotoxicity in VERO cells and their potential antiviral effects on DENV and CHIKV infections in those same cells. Methods Dry ethanolic extracts of M. americana and T. cymosa seeds were subjected to open column chromatographic fractionation, leading to the identification of four compounds: two coumarins, derived from M. americana; and lupeol acetate and voacangine derived from T. cymosa.. The cytotoxicity of each compound was subsequently assessed by the MTT method (at concentrations from 400 to 6.25 μg/mL). Pre- and post-treatment antiviral assays were performed at non-toxic concentrations; the resulting DENV inhibition was evaluated by Real-Time PCR, and the CHIKV inhibition was tested by the plating method. The results were analyzed by means of statistical analysis. Results The compounds showed low toxicity at concentrations ≤ 200 μg/mL. The compounds coumarin A and coumarin B, which are derived from the M. americana plant, significantly inhibited infection with both viruses during the implementation of the two experimental strategies employed here (post-treatment with inhibition percentages greater than 50%, p < 0.01; and pre-treatment with percentages of inhibition greater than 40%, p < 0.01). However, the lupeol acetate and voacangine compounds, which were derived from the T. cymosa plant, only significantly inhibited the DENV infection during the post-treatment strategy (at inhibition percentages greater than 70%, p < 0.01). Conclusion In vitro, the coumarins are capable of inhibiting infection by DENV and CHIKV (with inhibition percentages above 50% in different experimental strategies), which could indicate that these two compounds are potential antivirals for treating Dengue and Chikungunya fever. Additionally, lupeol acetate and voacangine efficiently inhibit infection with DENV, also turning them into promising antivirals for Dengue fever.application/pdf10.1186/s12906-017-1562-11472-6882https://repositorio.udes.edu.co/handle/001/3217engBMC Complementary and Alternative MedicineDerechos Reservados - Universidad de Santander, 2017info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://bmccomplementalternmed.biomedcentral.com/track/pdf/10.1186/s12906-017-1562-1Dengue VirusChikungunya virusAntiviralMammea americanaTabernaemontana cymosaAntiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infectionsArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85PublicationTHUMBNAILAntiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections.pdf.jpgAntiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections.pdf.jpgGenerated Thumbnailimage/jpeg1686https://repositorio.udes.edu.co/bitstreams/b5aadbd6-a48e-4879-90d5-eae59cc774ee/downloadd473d9bc26d320c1fb1a04a1c090ef7eMD54ORIGINALAntiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections.pdfAntiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections.pdfapplication/pdf315839https://repositorio.udes.edu.co/bitstreams/b7baac13-ef6e-45b1-8cec-7ea7da462b5c/downloadbf0c5f06dbc3dcbf0ed82721f746342aMD55LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/8afdf045-6c6d-4fdb-b91a-b34d0e9bfbfb/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTAntiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections.pdf.txtAntiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections.pdf.txtExtracted texttext/plain62807https://repositorio.udes.edu.co/bitstreams/669ea071-5f82-4476-b5f2-18141b995d4b/downloaded1d408b306217025b13c7142e13d46aMD53001/3217oai:repositorio.udes.edu.co:001/32172023-10-10 09:48:07.711https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Santander, 2017https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |