Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7
1 p.
- Autores:
-
Prada Prada, Sergio Alfonso
Flórez Castillo, Johanna-Marcela
Farfán García, Ana Elvira
Guzmán, Fanny
Hernández Peñaranda, Indira Paola
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2020
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/4693
- Acceso en línea:
- https://doi.org/10.1371/journal. pone.0229019
https://repositorio.udes.edu.co/handle/001/4693
- Palabra clave:
- Antimicrobial activity
Ib-M peptides
Escherichia coli
Antibiotics
- Rights
- restrictedAccess
- License
- Derechos Reservados - Prada-Prada et al., 2020
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Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
| title |
Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
| spellingShingle |
Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 Antimicrobial activity Ib-M peptides Escherichia coli Antibiotics |
| title_short |
Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
| title_full |
Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
| title_fullStr |
Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
| title_full_unstemmed |
Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
| title_sort |
Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7 |
| dc.creator.fl_str_mv |
Prada Prada, Sergio Alfonso Flórez Castillo, Johanna-Marcela Farfán García, Ana Elvira Guzmán, Fanny Hernández Peñaranda, Indira Paola |
| dc.contributor.author.spa.fl_str_mv |
Prada Prada, Sergio Alfonso Flórez Castillo, Johanna-Marcela Farfán García, Ana Elvira Guzmán, Fanny Hernández Peñaranda, Indira Paola |
| dc.contributor.corporatename.spa.fl_str_mv |
Universidad de Santander |
| dc.contributor.editor.spa.fl_str_mv |
Iddya Karunasagar, Nitte University, INDIA |
| dc.subject.proposal.eng.fl_str_mv |
Antimicrobial activity Ib-M peptides Escherichia coli Antibiotics |
| topic |
Antimicrobial activity Ib-M peptides Escherichia coli Antibiotics |
| description |
1 p. |
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2020 |
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2020-08-20T14:24:26Z |
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2020-08-20T14:24:26Z |
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2020-02-13 |
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Prada-Prada S, Flórez-Castillo J, Farfán- García A, Guzmán F, Hernández-Peñaranda I (2020) Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7. PLoS ONE 15 (2): e0229019. |
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https://doi.org/10.1371/journal. pone.0229019 |
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https://repositorio.udes.edu.co/handle/001/4693 |
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Prada-Prada S, Flórez-Castillo J, Farfán- García A, Guzmán F, Hernández-Peñaranda I (2020) Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7. PLoS ONE 15 (2): e0229019. |
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https://doi.org/10.1371/journal. pone.0229019 https://repositorio.udes.edu.co/handle/001/4693 |
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eng |
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eng |
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Plos One |
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PLOS ONE;February 13, 2020 |
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Derechos Reservados - Prada-Prada et al., 2020 |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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https://creativecommons.org/licenses/by-nc/4.0/ |
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Derechos Reservados - Prada-Prada et al., 2020 Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_16ec |
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San Francisco : Plos One, 2020. |
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Universidad de Santander |
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Prada Prada, Sergio Alfonsocc945093-702a-4e7e-b598-17852200db11-1Flórez Castillo, Johanna-Marcelae5b9135c-adb9-4f3f-adf9-eae7cd9e6abf-1Farfán García, Ana Elvira74472815-bd1a-4bf4-932b-0264dd209268-1Guzmán, Fanny0ea45d23-51b8-4cbd-9a49-4283f773574d-1Hernández Peñaranda, Indira Paolaebe71324-2be9-45d1-9cfa-eef589289ef3-1Universidad de SantanderIddya Karunasagar, Nitte University, INDIA2020-08-20T14:24:26Z2020-08-20T14:24:26Z2020-02-131 p.El desarrollo de nuevos péptidos antimicrobianos se ha convertido en una alternativa atractiva a los antibióticos convencionales debido a las crecientes tasas de resistencia a los fármacos microbianos. Ib-M corresponde a una familia de péptidos sintéticos catiónicos, de 20 aminoácidos de longitud, que han mostrado efecto inhibidor frente a la cepa no patógena Escherichia coli K-12. Este trabajo evaluó el potencial antimicrobiano de los péptidos Ib-M contra el E patógeno . coli O157: H7 utilizando una cepa de referencia y un aislado clínico. Los péptidos Ib-M mostraron actividad antibacteriana contra ambas cepas de E . coliO157: H7; la concentración mínima inhibitoria de los péptidos Ib-M osciló entre 1,6 y 12,5 µM y la concentración bactericida mínima osciló entre 3,7 y 22,9 µM, siendo Ib-M1 e Ib-M2 los péptidos que presentaron mayor efecto inhibidor. El ensayo de cinética de eliminación del tiempo mostró una reducción de la población bacteriana en más del 95% después de 4 horas de exposición a 1xMIC de Ib-M1. Se observó una baja citotoxicidad en células VERO con una concentración citotóxica del 50% en el rango de 197,5 a más de 400 μM. Todos los péptidos mostraron una estructura aleatoria en ambientes hidrófilos, excepto Ib-M1, y todos ellos pasaron a una estructura α-helicoidal cuando se incrementó la hidrofobicidad del medio. En conclusión, estos hallazgos apoyan el efecto antimicrobiano in vitro de los péptidos Ib-M contra las bacterias patógenas.E . coli O157: H7 y resultan ser moléculas prometedoras para el desarrollo de nuevas alternativas terapéuticas.The development of new antimicrobial peptides has become an attractive alternative to conventional antibiotics due to the increasing rates of microbial drug resistance. Ib-M corresponds to a family of cationic synthetic peptides, 20 amino acids in length, that have shown inhibitory effect against the non-pathogenic strain Escherichia coli K-12. This work evaluated the antimicrobial potential of Ib-M peptides against the pathogenic E. coli O157: H7 using a reference strain and a clinical isolate. The Ib-M peptides showed antibacterial activity against both strains of E. coli O157: H7; the minimum inhibitory concentration of Ib-M peptides ranged from 1.6 to 12.5 μM and the minimum bactericidal concentration ranged from 3.7 to 22.9 μM, being Ib-M1 and Ib-M2 the peptides that presented the highest inhibitory effect. Time-kill kinetics assay showed a reduction of the bacterial population by more than 95% after 4 hours of exposure to 1xMIC of Ib-M1. Low cytotoxicity was observed in VERO cells with 50% cytotoxic concentration in the range from 197.5 to more than 400 μM. All peptides showed a random structure in hydrophilic environments, except Ib-M1, and all of them transitioned to an α-helical structure when the hydrophobicity of the medium was increased. In conclusion, these findings support the in vitro antimicrobial effect of Ib-M peptides against the pathogenic bacteria E. coli O157: H7 and prove to be promising molecules for the development of new therapeutic alternatives.Ej. 1application/pdfPrada-Prada S, Flórez-Castillo J, Farfán- García A, Guzmán F, Hernández-Peñaranda I (2020) Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7. PLoS ONE 15 (2): e0229019.https://doi.org/10.1371/journal. pone.0229019https://repositorio.udes.edu.co/handle/001/4693engSan Francisco : Plos One, 2020.Plos OnePLOS ONE;February 13, 2020Derechos Reservados - Prada-Prada et al., 2020info:eu-repo/semantics/restrictedAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_16echttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0229019Antimicrobial activityIb-M peptidesEscherichia coliAntibioticsAntimicrobial activity of Ib-M peptides against Escherichia coli O157: H7Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85PublicationORIGINALAntimicrobial activity of Ib-M peptides against Escherichia coli O157.H7.pdfAntimicrobial activity of Ib-M peptides against Escherichia coli O157.H7.pdfapplication/pdf300103https://repositorio.udes.edu.co/bitstreams/829e25f5-797a-43e8-a3d1-8f6dd74054e9/download831e6040b97206e95bf2fdb8ceab039fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/dd0d2cb8-f643-407e-8b6a-5c9e56ca6fb5/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTAntimicrobial activity of Ib-M peptides against Escherichia coli O157.H7.pdf.txtAntimicrobial activity of Ib-M peptides against Escherichia coli O157.H7.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/fc3990fb-6fca-4869-9e35-6adc6cee0a31/download5dbe86c1111d64f45ba435df98fdc825MD53THUMBNAILAntimicrobial activity of Ib-M peptides against Escherichia coli O157.H7.pdf.jpgAntimicrobial activity of Ib-M peptides against Escherichia coli O157.H7.pdf.jpgGenerated Thumbnailimage/jpeg11775https://repositorio.udes.edu.co/bitstreams/19fc0e40-9afb-4379-ab6f-df354a4bb255/download44d76a72a0ed324a3672a720c653fb3fMD54001/4693oai:repositorio.udes.edu.co:001/46932023-10-11 09:08:08.914https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Prada-Prada et al., 2020https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |