Computational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells
Digital
- Autores:
-
Cruz-Laiton, Jenniffer
Suarez-Barrera, Miguel Orlando
Rondon-Villarreal, Paola
Olarte-Diaz, Andres
Guzman, Fanny
Visser, Lydia
Rueda-Forero, Nohora Juliana
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2021
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/6159
- Acceso en línea:
- https://doi.org/10.1042/BSR20211964
https://repositorio.udes.edu.co/handle/001/6159
- Palabra clave:
- Rights
- openAccess
- License
- © 2021 The Author(s)
| id |
RUDES2_6ca002b39993caed133f6d9b8fbb8410 |
|---|---|
| oai_identifier_str |
oai:repositorio.udes.edu.co:001/6159 |
| network_acronym_str |
RUDES2 |
| network_name_str |
Repositorio Universidad de Santander |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
Computational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells |
| title |
Computational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells |
| spellingShingle |
Computational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells |
| title_short |
Computational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells |
| title_full |
Computational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells |
| title_fullStr |
Computational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells |
| title_full_unstemmed |
Computational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells |
| title_sort |
Computational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells |
| dc.creator.fl_str_mv |
Cruz-Laiton, Jenniffer Suarez-Barrera, Miguel Orlando Rondon-Villarreal, Paola Olarte-Diaz, Andres Guzman, Fanny Visser, Lydia Rueda-Forero, Nohora Juliana |
| dc.contributor.author.none.fl_str_mv |
Cruz-Laiton, Jenniffer Suarez-Barrera, Miguel Orlando Rondon-Villarreal, Paola Olarte-Diaz, Andres Guzman, Fanny Visser, Lydia Rueda-Forero, Nohora Juliana |
| dc.contributor.researchgroup.spa.fl_str_mv |
Masira |
| description |
Digital |
| publishDate |
2021 |
| dc.date.issued.none.fl_str_mv |
2021-12-08 |
| dc.date.accessioned.none.fl_str_mv |
2022-02-25T13:51:58Z |
| dc.date.available.none.fl_str_mv |
2022-02-25T13:51:58Z |
| dc.type.spa.fl_str_mv |
Artículo de revista |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
http://purl.org/coar/resource_type/c_6501 |
| status_str |
publishedVersion |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1042/BSR20211964 |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.udes.edu.co/handle/001/6159 |
| url |
https://doi.org/10.1042/BSR20211964 https://repositorio.udes.edu.co/handle/001/6159 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.citationendpage.spa.fl_str_mv |
20 |
| dc.relation.citationissue.spa.fl_str_mv |
12 |
| dc.relation.citationstartpage.spa.fl_str_mv |
1 |
| dc.relation.citationvolume.spa.fl_str_mv |
41 |
| dc.relation.cites.none.fl_str_mv |
enniffer Cruz, Miguel Orlando Suárez-Barrera, Paola Rondón-Villarreal, Andrés Olarte-Diaz, Fanny Guzmán, Lydia Visser, Nohora Juliana Rueda-Forero; Computational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells. Biosci Rep 22 December 2021; 41 (12): BSR20211964. doi: https://doi.org/10.1042/BSR20211964 |
| dc.relation.indexed.spa.fl_str_mv |
Scopus |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Bioscience Reports |
| dc.rights.spa.fl_str_mv |
© 2021 The Author(s) |
| dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.creativecommons.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0/ |
| rights_invalid_str_mv |
© 2021 The Author(s) Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.extent.spa.fl_str_mv |
20 p |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.publisher.spa.fl_str_mv |
Bioscience Reports |
| dc.publisher.place.spa.fl_str_mv |
USA |
| dc.source.spa.fl_str_mv |
https://portlandpress.com/bioscirep/article/41/12/BSR20211964/230266/Computational-study-synthesis-and-evaluation-of |
| institution |
Universidad de Santander |
| bitstream.url.fl_str_mv |
https://repositorio.udes.edu.co/bitstreams/2fe54951-6bcf-45b6-9998-942c3262c23b/download https://repositorio.udes.edu.co/bitstreams/31f5515b-2cac-4c44-90a9-be17fa297e0f/download https://repositorio.udes.edu.co/bitstreams/527ef2f7-2378-41fb-9b4d-70788cac5cc6/download https://repositorio.udes.edu.co/bitstreams/cd3ddcca-c069-46ac-b84f-87953c641c77/download |
| bitstream.checksum.fl_str_mv |
57f5bf4ee87194b69c12aca75ad37484 38d94cf55aa1bf2dac1a736ac45c881c 5dbe86c1111d64f45ba435df98fdc825 8e1d22ba070b263c15204f3604cb37a1 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Universidad de Santander |
| repository.mail.fl_str_mv |
soporte@metabiblioteca.com |
| _version_ |
1814158756427071488 |
| spelling |
Cruz-Laiton, Jenniffera2084ed3-83c3-4862-958d-9caacd7cbec4-1Suarez-Barrera, Miguel Orlandof29bdf0a-38a9-4ff0-a936-6eca5b5e1e8a-1Rondon-Villarreal, Paola11c8f1f2-3d60-445c-926a-bdc78cf4e626-1Olarte-Diaz, Andres0c5293ba-048a-49c7-b13a-ca2b26e704d8-1Guzman, Fannye2162a90-5602-4223-8aa6-e2e1564ba308-1Visser, Lydiae51c095e-278c-427a-87e1-2005da5a6b4c-1Rueda-Forero, Nohora Juliana8a2dca8e-5326-487a-924d-96760e98b2bd-1Masira2022-02-25T13:51:58Z2022-02-25T13:51:58Z2021-12-08DigitalParasporin-2Aa1 (PS2Aa1) is a toxic protein of 37 KDa (30 kDa, activated form produced by proteolysis) that was shown to be cytotoxic against specific human cancer cells, although its mechanism of action has not been elucidated yet. In order to study the role of some native peptide fragments of proteins on anticancer activity, here we investigated the cytotoxic effect of peptide fragments from domain-1 of PS2Aa1 and one of the loops present in the binding region of the virus spike protein from Alphacoronavirus (HCoV-229E), the latter according to scientific reports, who showed interaction with the human APN (h-APN) receptor, evidence corroborated through computational simulations, and thus being possible active against colon cancer cells. Peptides namely P264-G274, Loop1-PS2Aa, and Loop2-PS2Aa were synthesized using the Fmoc solid-phase synthesis and characterized by mass spectrometry (MS). Additionally, one region from loop 1 of HCoV-229E, Loop1-HCoV-229E, was also synthesized and characterized. The A4W-GGN5 anticancer peptide and 5-fluorouracil (5-FU) were taken as a control in all experiments. Circular dichroism revealed an α-helix structure for the peptides derived from PS2Aa1 (P264-G274, Loop1-PS2Aa, and Loop2-PS2Aa) and β-laminar structure for the peptide derived from Alphacoronavirus spike protein Loop1-HCoV-229E. Peptides showed a hemolysis percentage of less than 20% at 100 μM concentration. Besides, peptides exhibited stronger anticancer activity against SW480 and SW620 cells after exposure for 48 h. Likewise, these compounds showed significantly lower toxicity against normal cells CHO-K1. The results suggest that native peptide fragments from Ps2Aa1 may be optimized as a novel potential cancer-therapeutic agents.Ciencias Médicas y de la Salud20 papplication/pdfhttps://doi.org/10.1042/BSR20211964https://repositorio.udes.edu.co/handle/001/6159engBioscience ReportsUSA2012141enniffer Cruz, Miguel Orlando Suárez-Barrera, Paola Rondón-Villarreal, Andrés Olarte-Diaz, Fanny Guzmán, Lydia Visser, Nohora Juliana Rueda-Forero; Computational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells. Biosci Rep 22 December 2021; 41 (12): BSR20211964. doi: https://doi.org/10.1042/BSR20211964ScopusBioscience Reports© 2021 The Author(s)info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://portlandpress.com/bioscirep/article/41/12/BSR20211964/230266/Computational-study-synthesis-and-evaluation-ofComputational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cellsArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Todas las AudienciasPublicationORIGINALComputational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells.pdfComputational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells.pdfapplication/pdf235481https://repositorio.udes.edu.co/bitstreams/2fe54951-6bcf-45b6-9998-942c3262c23b/download57f5bf4ee87194b69c12aca75ad37484MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/31f5515b-2cac-4c44-90a9-be17fa297e0f/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTComputational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells.pdf.txtComputational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/527ef2f7-2378-41fb-9b4d-70788cac5cc6/download5dbe86c1111d64f45ba435df98fdc825MD53THUMBNAILComputational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells.pdf.jpgComputational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells.pdf.jpgGenerated Thumbnailimage/jpeg12987https://repositorio.udes.edu.co/bitstreams/cd3ddcca-c069-46ac-b84f-87953c641c77/download8e1d22ba070b263c15204f3604cb37a1MD54001/6159oai:repositorio.udes.edu.co:001/61592023-10-09 16:42:36.03https://creativecommons.org/licenses/by-nc/4.0/© 2021 The Author(s)https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |