Alteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae)

Digital

Autores:
Rincón-Barón, Edgar Javier
Grisales-Echeverri, Claudia
Cuaran, Viviana Lucia
Cardona-B., Nadya Lorena
Tipo de recurso:
Article of journal
Fecha de publicación:
2020
Institución:
Universidad de Santander
Repositorio:
Repositorio Universidad de Santander
Idioma:
eng
spa
OAI Identifier:
oai:repositorio.udes.edu.co:001/6716
Acceso en línea:
https://repositorio.udes.edu.co/handle/001/6716
Palabra clave:
Biotrophs
Histochemistry
Hydrangeaceae
Plant histopathology
Papillae
Powdery mildew
Rights
closedAccess
License
http://purl.org/coar/access_right/c_14cb
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dc.title.spa.fl_str_mv Alteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae)
dc.title.alternative.spa.fl_str_mv Anatomical and histochemical alterations caused by powdery mildew on hydrangea mac-rophylla (Hydrangeaceae) leaves
title Alteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae)
spellingShingle Alteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae)
Biotrophs
Histochemistry
Hydrangeaceae
Plant histopathology
Papillae
Powdery mildew
title_short Alteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae)
title_full Alteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae)
title_fullStr Alteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae)
title_full_unstemmed Alteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae)
title_sort Alteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae)
dc.creator.fl_str_mv Rincón-Barón, Edgar Javier
Grisales-Echeverri, Claudia
Cuaran, Viviana Lucia
Cardona-B., Nadya Lorena
dc.contributor.author.none.fl_str_mv Rincón-Barón, Edgar Javier
Grisales-Echeverri, Claudia
Cuaran, Viviana Lucia
Cardona-B., Nadya Lorena
dc.contributor.researchgroup.spa.fl_str_mv Microbiota
dc.subject.proposal.eng.fl_str_mv Biotrophs
Histochemistry
Hydrangeaceae
Plant histopathology
Papillae
Powdery mildew
topic Biotrophs
Histochemistry
Hydrangeaceae
Plant histopathology
Papillae
Powdery mildew
description Digital
publishDate 2020
dc.date.issued.none.fl_str_mv 2020-07-03
dc.date.accessioned.none.fl_str_mv 2022-05-06T13:28:59Z
dc.date.available.none.fl_str_mv 2022-05-06T13:28:59Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.identifier.doi.none.fl_str_mv 10.15517/RBT.V68I3.40431
dc.identifier.eissn.spa.fl_str_mv 2215-2075
dc.identifier.issn.spa.fl_str_mv 0034-7744
dc.identifier.uri.none.fl_str_mv https://repositorio.udes.edu.co/handle/001/6716
identifier_str_mv 10.15517/RBT.V68I3.40431
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url https://repositorio.udes.edu.co/handle/001/6716
dc.language.iso.spa.fl_str_mv eng
spa
language eng
spa
dc.relation.citationendpage.spa.fl_str_mv 976
dc.relation.citationissue.spa.fl_str_mv 3
dc.relation.citationstartpage.spa.fl_str_mv 959
dc.relation.citationvolume.spa.fl_str_mv 68
dc.relation.cites.none.fl_str_mv Rincón-Baron, E.J., Grisales-Echeverri, C., Cuaran, V.L., & Cardona-B., N.L. (2020). Alteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae). Revista de Biología Tropical, 68(3), 959-976. ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075
dc.relation.indexed.spa.fl_str_mv Scopus
dc.relation.ispartofjournal.spa.fl_str_mv Revista de Biología Tropical
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dc.format.extent.spa.fl_str_mv 18 p
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.place.spa.fl_str_mv Costa Rica
institution Universidad de Santander
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spelling Rincón-Barón, Edgar Javier5d5018cb-7941-4cb4-9aec-41d9139a6ad5-1Grisales-Echeverri, Claudia5a485971-7349-421a-9d61-59f6df4ac1dd-1Cuaran, Viviana Lucia032c7465-c1d9-4fab-bb87-c4a091c60d6a-1Cardona-B., Nadya Lorenaf329afb0-2b56-4518-a8c2-69d19aa1de75-1Microbiota2022-05-06T13:28:59Z2022-05-06T13:28:59Z2020-07-03DigitalAnatomical and histochemical alterations caused by powdery mildew on Hydrangea mac-rophylla (Hydrangeaceae) leaves. Introduction: There are few studies concerning the morpho-anatomical and histochemical alterations caused by powdery mildew in H. macrophylla leaves in the scientific literature. Objective: To describe and analyze anatomical and histochemical aspects of this pathosystem. Methods: More than 90 leaves of H. macrophylla (both healthy and infected leaves by powdery mildew) were collected in the nursery El Jardín del Eden, Rionegro, Antioquia, Colombia. To carry out the identification of the mycopatho-gen, sections were stained with Lactophenol Blue, and contrasted with specialized taxonomic keys. Transverse fragments 1 cm thick were fixed in a mixture of formalin, alcohol, and acetic acid. These were subsequently dehydrated using an ethanol series, clarified in Xylene, and finally embedded in Paraplast plus® to obtain 5 μm sections. Schiff’s periodic acid reaction (PAS) was used to detect structural and reserve polysaccharides, Ruthenium Red for pectins, Ponseau S and Lacmoid for callose, ferric chloride for polyphenols, Sudan Black for lipids and Uvitex 2B-Hematoxylin for chitin. The sections were observed using a Nikon 80i eclipse® pho-ton microscope, with Uvitex 2B-Hematoxylin-stained sections examined by epifluorescence using a UV-2A filter. For the observation and description of the samples by scanning electron microscopy, healthy and infected leaves were fixed and dehydrated in 100 % methanol, critical point dried, and coated with gold. Results:H. macrophylla leaves are isobilateral and homobaric, with adaxial and abaxial epidermis of a single cellular layer. The palisade parenchyma consists of a layer of short cells, while the spongy parenchyma forms 6 to 7 cellular layers. All vascular bundles in the leaf blade are closed collaterals. Abundant idioblasts with raphides may be observed in the mesophyll, and starch is the main reserve carbohydrate present in the tissues. The leaves are hypostomatic and exhibit a paracytic pattern of superficial stomata which possess large substomatal cavities. The morphological data observed indicate that the mycopathogen is related to the genus Erysiphe. The epidermal cells affected by the pathogen exhibit thickened walls, granular cytoplasm, and papillae or cell wall appositions in the outer periclinal walls. With the deterioration of the epidermis, the underlying tissues are affected and become necrotic. Histochemical test indicate that infected plants thicken and reinforce their epidermal cell walls with primary wall materials; primarily cutin, pectins, and callose. When stained with Sudan Black, the presence of dark-colored agglomerates in the cytoplasm of epidermal cells may be related to plant defense mechanisms; and those observed in mesophilic cells to the disorganization of membrane systems. Polyphenols accumulate in the cytoplasm of infected epidermal cells. The fungal material present in epidermal tissues was clearly differenti-ated when stained with fluorochrome to detect chitin. Conclusions: Species of the genus Erysiphe are causative agents of powdery mildew in H. macrophylla. Necrosis of the epidermal cells is observed in response to the mycopathogen, possibly due to hypersensitive response.Ciencias Exactas y Naturales, Biotecnología Agroambiente y Salud18 papplication/pdf10.15517/RBT.V68I3.404312215-20750034-7744https://repositorio.udes.edu.co/handle/001/6716engspaCosta Rica976395968Rincón-Baron, E.J., Grisales-Echeverri, C., Cuaran, V.L., & Cardona-B., N.L. (2020). Alteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae). Revista de Biología Tropical, 68(3), 959-976. ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075ScopusRevista de Biología Tropicalinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbBiotrophsHistochemistryHydrangeaceaePlant histopathologyPapillaePowdery mildewAlteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae)Anatomical and histochemical alterations caused by powdery mildew on hydrangea mac-rophylla (Hydrangeaceae) leavesArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Todas las AudienciasPublicationORIGINALAlteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae).pdfAlteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae).pdfapplication/pdf238140https://repositorio.udes.edu.co/bitstreams/03884845-7306-4a31-84e2-0f17b0071cea/downloadf827ebc72b1ce6e485236552cd71ec92MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/134c8c4c-d33e-4fb6-8edc-ed0236064c66/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTAlteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae).pdf.txtAlteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae).pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/71dd1eec-fc8b-4d8c-9377-0821d1206556/download5dbe86c1111d64f45ba435df98fdc825MD53THUMBNAILAlteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae).pdf.jpgAlteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae).pdf.jpgGenerated Thumbnailimage/jpeg12022https://repositorio.udes.edu.co/bitstreams/0b09578f-d679-462f-9566-bb16f3485e2f/downloada662b29c1665b4dc4656dc5983aef81aMD54001/6716oai:repositorio.udes.edu.co:001/67162023-10-11 11:03:35.671https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo=

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