Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing
Digital
- Autores:
-
Higuera, Adriana
Herrera, Giovanny
Jimenez, Paula
Garcia-Corredor, Diego
Pulido-Medellín, Martin
Bulla-Castañeda, Diana M.
Pinilla, Juan Carlos
Moreno-Pérez, Darwin A.
Maloney, Jenny G.
Santin, Mónica
Ramirez, Juan David
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2021
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/6345
- Acceso en línea:
- https://doi.org/10.3389/fvets.2021.732129
https://repositorio.udes.edu.co/handle/001/6345
- Palabra clave:
- Blastocystis
Colombia
Farm animals
Next generation amplicon sequencing
Mixed infection
MinION
Subtypes
- Rights
- openAccess
- License
- Atribución 4.0 Internacional (CC BY 4.0)
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Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing |
| title |
Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing |
| spellingShingle |
Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing Blastocystis Colombia Farm animals Next generation amplicon sequencing Mixed infection MinION Subtypes |
| title_short |
Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing |
| title_full |
Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing |
| title_fullStr |
Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing |
| title_full_unstemmed |
Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing |
| title_sort |
Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing |
| dc.creator.fl_str_mv |
Higuera, Adriana Herrera, Giovanny Jimenez, Paula Garcia-Corredor, Diego Pulido-Medellín, Martin Bulla-Castañeda, Diana M. Pinilla, Juan Carlos Moreno-Pérez, Darwin A. Maloney, Jenny G. Santin, Mónica Ramirez, Juan David |
| dc.contributor.author.none.fl_str_mv |
Higuera, Adriana Herrera, Giovanny Jimenez, Paula Garcia-Corredor, Diego Pulido-Medellín, Martin Bulla-Castañeda, Diana M. Pinilla, Juan Carlos Moreno-Pérez, Darwin A. Maloney, Jenny G. Santin, Mónica Ramirez, Juan David |
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GICAS |
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Blastocystis Colombia Farm animals Next generation amplicon sequencing Mixed infection MinION Subtypes |
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Blastocystis Colombia Farm animals Next generation amplicon sequencing Mixed infection MinION Subtypes |
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Digital |
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2021 |
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2021-08-24 |
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2022-03-16T15:49:57Z |
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eng |
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Higuera A, Herrera G, Jimenez P, García-Corredor D, Pulido-Medellín M, Bulla-Castañeda DM, Pinilla JC, Moreno-Pérez DA, Maloney JG, Santín M and Ramírez JD (2021) Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing. Front. Vet. Sci. 8:732129. doi: 10.3389/fvets.2021.732129 |
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Scopus |
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Frontiers in Veterinary Science |
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Atribución 4.0 Internacional (CC BY 4.0) |
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Higuera, Adriana7f7c14e0-d84a-4ae1-980f-9b501bfd7de8-1Herrera, Giovannya8715c58-332d-4c96-b1f7-74ee2125ff42-1Jimenez, Paulae412ae62-e038-4f48-a27a-f62dff2f9d26-1Garcia-Corredor, Diegob982d150-ff88-4ea0-9287-fdb80707cd5bPulido-Medellín, Martina56f05bc-df56-4d94-a9bc-cec178f969d7-1Bulla-Castañeda, Diana M.584a4bdc-e5eb-4ecc-ac3e-2527c08c4b31-1Pinilla, Juan Carlos94e517a6-ccf3-4017-9d1a-063cad769aec-1Moreno-Pérez, Darwin A.650c9d66-9731-47b2-a646-8d5234b0fae7-1Maloney, Jenny G.6c6127b4-b9c8-457e-8db3-d9e8e2a08c59-1Santin, Mónicaede3171d-7c36-4255-8aa8-c0773c93bde9Ramirez, Juan Davidafaa63f5-a60d-445a-bd10-fd3084bb2b09GICAS2022-03-16T15:49:57Z2022-03-16T15:49:57Z2021-08-24DigitalBlastocystis is frequently reported in fecal samples from animals and humans worldwide, and a variety of subtypes (STs) have been observed in wild and domestic animals. In Colombia, few studies have focused on the transmission dynamics and epidemiological importance of Blastocystis in animals. In this study, we characterized the frequency and subtypes of Blastocystis in fecal samples of domestic animals including pigs, minipigs, cows, dogs, horses, goats, sheep, and llama from three departments of Colombia. Of the 118 fecal samples included in this study 81.4% (n = 96) were positive for Blastocystis using a PCR that amplifies a fragment of the small subunit ribosomal RNA (SSU rRNA) gene. PCR positive samples were sequenced by next generation amplicon sequencing (NGS) to determine subtypes. Eleven subtypes were detected, ten previously reported, ST5 (50.7%), ST10 (47.8%), ST25 (34.3%), ST26 (29.8%), ST21 (22.4%), ST23 (22.4%), ST1 (17.9%), ST14 (16.4%), ST24 (14.9%), ST3 (7.5%), and a novel subtype, named ST32 (3.0%). Mixed infection and/or intra -subtype variations were identified in most of the samples. Novel ST32 was observed in two samples from a goat and a cow. To support novel subtype designation, a MinION based sequencing strategy was used to generate the full-length of the SSU rRNA gene. Comparison of full-length nucleotide sequences with those from current valid subtypes supported the designation of ST32. This is the first study in Colombia using NGS to molecularly characterize subtypes of Blastocystis in farm animals. A great diversity of subtypes was observed in domestic animals including subtypes previously identified in humans. Additionally, subtype overlap between the different hosts examined in this study were observed. These findings highlight the presence of Blastocystis subtypes with zoonotic potential in farm animals indicating that farm animals could play a role in transmission to humans.Medicina Veterinaria11 papplication/pdfhttps://doi.org/10.3389/fvets.2021.732129https://repositorio.udes.edu.co/handle/001/6345engSuiza11124Higuera A, Herrera G, Jimenez P, García-Corredor D, Pulido-Medellín M, Bulla-Castañeda DM, Pinilla JC, Moreno-Pérez DA, Maloney JG, Santín M and Ramírez JD (2021) Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing. Front. Vet. Sci. 8:732129. doi: 10.3389/fvets.2021.732129ScopusFrontiers in Veterinary ScienceCopyright © 2021 Higuera, Herrera, Jimenez, García-Corredor, Pulido-Medellín, Bulla-Castañeda, Pinilla, Moreno-Pérez, Maloney, Santín and Ramírez.info:eu-repo/semantics/openAccessAtribución 4.0 Internacional (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2https://www.frontiersin.org/articles/10.3389/fvets.2021.732129/fullBlastocystisColombiaFarm animalsNext generation amplicon sequencingMixed infectionMinIONSubtypesIdentification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation SequencingArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Todas las AudienciasPublicationORIGINALIdentification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing.pdfIdentification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing.pdfapplication/pdf230258https://repositorio.udes.edu.co/bitstreams/c55c7836-60b0-45a1-8c8d-270d18a0c99a/downloadb2e9900b14be73bb193deb60475f0a29MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/7cf3b054-189e-44e5-9f5c-0803909e3a1e/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTIdentification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing.pdf.txtIdentification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/daf3602c-b08e-45e6-907b-efa968f037ab/download5dbe86c1111d64f45ba435df98fdc825MD53THUMBNAILIdentification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing.pdf.jpgIdentification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing.pdf.jpgGenerated Thumbnailimage/jpeg11529https://repositorio.udes.edu.co/bitstreams/61b23082-7dbf-42a0-bd5c-da94337e857b/download8cd748a3df919b19457d356403498ecdMD54001/6345oai:repositorio.udes.edu.co:001/63452022-10-25 11:25:59.392https://creativecommons.org/licenses/by/4.0/Copyright © 2021 Higuera, Herrera, Jimenez, García-Corredor, Pulido-Medellín, Bulla-Castañeda, Pinilla, Moreno-Pérez, Maloney, Santín and Ramírez.https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |