Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America : A possible new CPV-2a is emerging?

6 p.

Autores:
Duque-García, Yeison
Echeverri-Zuluaga, Manuela
Trejos Suárez, Juanita
Ruiz Saenz, Julian
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Universidad de Santander
Repositorio:
Repositorio Universidad de Santander
Idioma:
eng
OAI Identifier:
oai:repositorio.udes.edu.co:001/3427
Acceso en línea:
https://repositorio.udes.edu.co/handle/001/3427
Palabra clave:
Antigenic variant
Hemorrhagic enteritis
Prevalence
Sequencing
Rights
openAccess
License
Derechos Reservados - Universidad de Santander, 2017
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dc.title.eng.fl_str_mv Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America : A possible new CPV-2a is emerging?
title Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America : A possible new CPV-2a is emerging?
spellingShingle Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America : A possible new CPV-2a is emerging?
Antigenic variant
Hemorrhagic enteritis
Prevalence
Sequencing
title_short Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America : A possible new CPV-2a is emerging?
title_full Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America : A possible new CPV-2a is emerging?
title_fullStr Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America : A possible new CPV-2a is emerging?
title_full_unstemmed Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America : A possible new CPV-2a is emerging?
title_sort Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America : A possible new CPV-2a is emerging?
dc.creator.fl_str_mv Duque-García, Yeison
Echeverri-Zuluaga, Manuela
Trejos Suárez, Juanita
Ruiz Saenz, Julian
dc.contributor.author.spa.fl_str_mv Duque-García, Yeison
Echeverri-Zuluaga, Manuela
Trejos Suárez, Juanita
Ruiz Saenz, Julian
dc.subject.proposal.eng.fl_str_mv Antigenic variant
Hemorrhagic enteritis
Prevalence
Sequencing
topic Antigenic variant
Hemorrhagic enteritis
Prevalence
Sequencing
description 6 p.
publishDate 2017
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dc.date.accessioned.spa.fl_str_mv 2019-07-18T19:06:45Z
dc.date.available.spa.fl_str_mv 2019-07-18T19:06:45Z
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dc.identifier.doi.spa.fl_str_mv 10.1016/j.vetmic.2016.12.039
dc.identifier.issn.spa.fl_str_mv 0378-1135
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0378-1135
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dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartof.eng.fl_str_mv Veterinary Microbiology
dc.rights.spa.fl_str_mv Derechos Reservados - Universidad de Santander, 2017
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spelling Duque-García, Yeisona671a691-68b4-448f-93d1-0dc2add7b534-1Echeverri-Zuluaga, Manuela152fa47c-5848-4560-9117-38b16f02e19b-1Trejos Suárez, Juanitad1ff80c8-4661-4ad3-8cc1-ac4b0fe1abe3-1Ruiz Saenz, Julian827f4fa2-52e4-40a5-b081-9e1d95950338-12019-07-18T19:06:45Z2019-07-18T19:06:45Z2017-016 p.Since its identification in 1978, Canine parvovirus type 2 (CPV-2) has been considered a pathogen of great importance in the canine population because it causes severe enteritis with high mortality rates in pups. CPV-2 is a virus belonging to the family Parvoviridae. Currently, there are three described antigenic variants (CPV-2a, CPV-2b, and CPV-2c). CPV-2c is an emerging virus that is seen as a global health hazard. The objective of this work was to confirm the presence of CPV-2 in dogs with acute gastroenteritis compatible with parvovirus and to molecularly characterize the antigenic variants circulating in two regions of Colombia. An analytical cross-sectional study was conducted with fecal samples collected from 71 dogs showing signs of acute diarrhea. The samples were processed and polymerase chain reaction (PCR), restriction fragment length polymorphism analysis (RFLP), sequencing and phylogenetic analysis was performed to detect and characterize CPV. A total of 70.42% of the individuals were confirmed positive for CPV-2. Statistically differences were found in the presentation of CPV-2 between the evaluated regions. Phylogenetic analyses confirmed the presence of the antigenic variants CPV-2a/2b. Moreover, we found the presence of two conserved substitutions Asn428Asp and Ala514Ser in the VP2 protein suggesting the presence of a possible new CPV-2a variant circulating in Colombia. This study demonstrates the importance of the CPV 2a/2b in the region and highlights the importance of performing molecular studies for the early detection of new antigenic variants of CPV-2.application/pdf10.1016/j.vetmic.2016.12.0390378-1135https://repositorio.udes.edu.co/handle/001/3427engVeterinary MicrobiologyDerechos Reservados - Universidad de Santander, 2017info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://www.sciencedirect.com/science/article/pii/S0378113516305739?via%3DihubAntigenic variantHemorrhagic enteritisPrevalenceSequencingPrevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America : A possible new CPV-2a is emerging?Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85PublicationBRANDED_PREVIEWPrevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America. 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