An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cells
Digital
- Autores:
-
Jureczek, Justyna
Bergmann, Ralf
Berndt, Nicole
Koristka, Stefanie
Kegler, Alexandra
Puentes-Cala, Edinson
Soto, Javier-Andrés
Arndt, Claudia
Bachmann, Michael
Feldmann, Anja
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2019
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/5614
- Acceso en línea:
- https://doi.org/10.1038/s41598-019-47044-4
https://repositorio.udes.edu.co/handle/001/5614
- Palabra clave:
- Rights
- openAccess
- License
- The Author(s) 2019
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An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cells |
| title |
An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cells |
| spellingShingle |
An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cells |
| title_short |
An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cells |
| title_full |
An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cells |
| title_fullStr |
An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cells |
| title_full_unstemmed |
An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cells |
| title_sort |
An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cells |
| dc.creator.fl_str_mv |
Jureczek, Justyna Bergmann, Ralf Berndt, Nicole Koristka, Stefanie Kegler, Alexandra Puentes-Cala, Edinson Soto, Javier-Andrés Arndt, Claudia Bachmann, Michael Feldmann, Anja |
| dc.contributor.author.none.fl_str_mv |
Jureczek, Justyna Bergmann, Ralf Berndt, Nicole Koristka, Stefanie Kegler, Alexandra Puentes-Cala, Edinson Soto, Javier-Andrés Arndt, Claudia Bachmann, Michael Feldmann, Anja |
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eng |
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(2019) 9:10547 |
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1 |
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Published On line: 29 July 2019 |
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Jureczek, J., Bergmann, R., Berndt, N. et al. An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cells. Sci Rep 9, 10547 (2019). https://doi.org/10.1038/s41598-019-47044-4 |
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Inglaterra |
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https://www.nature.com/articles/s41598-019-47044-4 |
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Jureczek, Justynad6b5893c-915d-43b6-b12a-4aa10ef1c666-1Bergmann, Ralf42ee2bae-2c97-4474-89fe-e2149df4bd4f-1Berndt, Nicole71097c9d-2605-4e5e-b873-ed1513f6c7f6-1Koristka, Stefanied44d9925-f9c2-423c-ac3a-b820215a6323-1Kegler, Alexandrad66f48e1-cb93-45f8-a232-0b1bae45efb6-1Puentes-Cala, Edinson9649c845-5bef-4bab-ab46-8124f11616c0-1Soto, Javier-Andrésd924ce22-9555-4607-bfd1-cb5724e4183e-1Arndt, Claudia15aa7eca-d54a-4daa-b28c-a56412622780-1Bachmann, Michael08a94c5e-ed5b-4e74-96a8-2fcd547a5a78-1Feldmann, Anjaff85e74e-e8e8-4ad1-9f31-a7048c48b0f5-12021-09-14T20:32:24Z2021-09-14T20:32:24Z2019-07-29DigitalRecently, we established the controllable modular UniCAR platform technology to advance the efficacy and safety of CAR T cell therapy. The UniCAR system is composed of (i) target modules (TMs) and (ii) UniCAR armed T cells. TMs are bispecific molecules that are able to bind to the tumor cell surface and simultaneously to UniCAR T cells. For interaction with UniCAR T cells, TMs contain a peptide epitope sequence which is recognised by UniCAR T cells. So far, a series of TMs against a variety of tumor targets including against the prostate stem cell antigen (PSCA) were constructed and functionally characterised. In order to facilitate their purification all these TMs are expressed as recombinant proteins equipped with an oligo-His-tag. The aim of the here presented manuscript was to learn whether or not the oligo-His-tag of the TM influences the UniCAR system. For this purpose, we constructed TMs against PSCA equipped with or lacking an oligo-His-tag. Both TMs were compared side by side including for functionality and biodistribution. According to our data, an oligo-His-tag of a UniCAR TM has only little if any effect on its binding affinity, in vitro and in vivo killing capability and in vivo biodistribution.1 ed.1 papplication/pdfhttps://doi.org/10.1038/s41598-019-47044-4https://repositorio.udes.edu.co/handle/001/5614engInglaterra15(2019) 9:105471Published On line: 29 July 2019Jureczek, J., Bergmann, R., Berndt, N. et al. An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cells. Sci Rep 9, 10547 (2019). https://doi.org/10.1038/s41598-019-47044-4Scientific ReportsThe Author(s) 2019info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://www.nature.com/articles/s41598-019-47044-4An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cellsArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTCASOinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Todas las AudienciasPublicationORIGINALAn_oligo-His-tag_of_a_targeting_module_does_not_influence_its_biodistribution_and_the_retargeting_capabilities_of_UniCAR_T_cells.pdfAn_oligo-His-tag_of_a_targeting_module_does_not_influence_its_biodistribution_and_the_retargeting_capabilities_of_UniCAR_T_cells.pdfapplication/pdf162449https://repositorio.udes.edu.co/bitstreams/f2daecc2-9004-40cd-90bf-cfc0029c3e50/download5509b710ef820b21033afb4ba528e391MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/b04525dc-1fee-4907-87a5-8948b780e441/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTAn_oligo-His-tag_of_a_targeting_module_does_not_influence_its_biodistribution_and_the_retargeting_capabilities_of_UniCAR_T_cells.pdf.txtAn_oligo-His-tag_of_a_targeting_module_does_not_influence_its_biodistribution_and_the_retargeting_capabilities_of_UniCAR_T_cells.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/00c5617d-6f64-4e1f-bdc0-89cadf1bfc5f/download5dbe86c1111d64f45ba435df98fdc825MD53THUMBNAILAn_oligo-His-tag_of_a_targeting_module_does_not_influence_its_biodistribution_and_the_retargeting_capabilities_of_UniCAR_T_cells.pdf.jpgAn_oligo-His-tag_of_a_targeting_module_does_not_influence_its_biodistribution_and_the_retargeting_capabilities_of_UniCAR_T_cells.pdf.jpgGenerated Thumbnailimage/jpeg12996https://repositorio.udes.edu.co/bitstreams/a649b6fb-14bb-464a-911c-72a21f1f4e7b/download00320bf8c260faad7b1883afa86f0457MD54001/5614oai:repositorio.udes.edu.co:001/56142023-10-09 10:16:52.038https://creativecommons.org/licenses/by-nc/4.0/The Author(s) 2019https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |