A bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157:H7 in an aqueous matrix
Digital
- Autores:
-
Ropero-Vega, Jose Luis
Redondo-Ortega, Joshua Felipe
Galvis-Curubo, Yuli Juliana
Rondon-Villarreal, Paola
Florez-Castillo, Johanna Marcela
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2021
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/6167
- Acceso en línea:
- https://doi.org/10.3390/molecules26092559
https://repositorio.udes.edu.co/handle/001/6167
- Palabra clave:
- Pathogen
PEPTIR-1.0
Screen-printed electrodes
Gold nanoparticles
Electrodeposition
Limit of detection
Selectivity
- Rights
- openAccess
- License
- Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland
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A bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157:H7 in an aqueous matrix |
| title |
A bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157:H7 in an aqueous matrix |
| spellingShingle |
A bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157:H7 in an aqueous matrix Pathogen PEPTIR-1.0 Screen-printed electrodes Gold nanoparticles Electrodeposition Limit of detection Selectivity |
| title_short |
A bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157:H7 in an aqueous matrix |
| title_full |
A bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157:H7 in an aqueous matrix |
| title_fullStr |
A bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157:H7 in an aqueous matrix |
| title_full_unstemmed |
A bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157:H7 in an aqueous matrix |
| title_sort |
A bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157:H7 in an aqueous matrix |
| dc.creator.fl_str_mv |
Ropero-Vega, Jose Luis Redondo-Ortega, Joshua Felipe Galvis-Curubo, Yuli Juliana Rondon-Villarreal, Paola Florez-Castillo, Johanna Marcela |
| dc.contributor.author.none.fl_str_mv |
Ropero-Vega, Jose Luis Redondo-Ortega, Joshua Felipe Galvis-Curubo, Yuli Juliana Rondon-Villarreal, Paola Florez-Castillo, Johanna Marcela |
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Cibas |
| dc.subject.proposal.eng.fl_str_mv |
Pathogen PEPTIR-1.0 Screen-printed electrodes Gold nanoparticles Electrodeposition Limit of detection Selectivity |
| topic |
Pathogen PEPTIR-1.0 Screen-printed electrodes Gold nanoparticles Electrodeposition Limit of detection Selectivity |
| description |
Digital |
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2021 |
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2021-04-28 |
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2022-02-25T16:56:17Z |
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2022-02-25T16:56:17Z |
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https://doi.org/10.3390/molecules26092559 |
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https://doi.org/10.3390/molecules26092559 https://repositorio.udes.edu.co/handle/001/6167 |
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eng |
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eng |
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2559 |
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Ropero-Vega, J.L.; Redondo-Ortega, J.F.; Galvis-Curubo, Y.J.; Rondón-Villarreal, P.; Flórez-Castillo, J.M. A Bioinspired Peptide in TIR Protein as Recognition Molecule on Electrochemical Biosensors for the Detection of E. coli O157:H7 in an Aqueous Matrix. Molecules 2021, 26, 2559. https:// doi.org/10.3390/molecules26092559 |
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Scopus |
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Molecules |
| dc.rights.spa.fl_str_mv |
Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución 4.0 Internacional (CC BY 4.0) |
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17 p |
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application/pdf |
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Sulka, Grzegorz D. Bogdanowicz, Robert |
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Suiza |
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https://www.mdpi.com/1420-3049/26/9/2559 |
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Ropero-Vega, Jose Luisb0cce9cf-2182-48ea-9908-2c06e54f295c-1Redondo-Ortega, Joshua Felipef1315901-ec26-4bc7-b1c8-630ee0cb4577-1Galvis-Curubo, Yuli Julianae89e07dd-d7ab-4b8d-8ed0-94a667152232-1Rondon-Villarreal, Paola11c8f1f2-3d60-445c-926a-bdc78cf4e626-1Florez-Castillo, Johanna Marcelab7d58b0e-4d82-43f8-8613-734e2b15155b-1Cibas2022-02-25T16:56:17Z2022-02-25T16:56:17Z2021-04-28DigitalCurrently, the detection of pathogens such as Escherichia coli through instrumental alternatives with fast response and excellent sensitivity and selectivity are being studied. Biosensors are systems consisting of nanomaterials and biomolecules that exhibit remarkable properties such as simplicity, portable, affordable, user‑friendly, and deliverable to end‑users. For this, in this work we report for the first time, to our knowledge, the bioinformatic design of a new peptide based on TIR protein, a receptor of Intimin membrane protein which is characteristic of E. coli. This peptide (named PEPTIR‑1.0) was used as recognition element in a biosensor based on AuNPs‑modified screen‑printed electrodes for the detection of E. coli. The morphological and electrochemical characteristics of the biosensor obtained were studied. Results show that the biosensor can detect the bacteria with limits of detection and quantification of 2 and 6 CFU/mL, respectively. Moreover, the selectivity of the system is statistically significant towards the detection of the pathogen in the presence of other microorganisms such as P. aeruginosa and S. aureus. This makes this new PEPTIR‑1.0 based biosensor can be used in the rapid, sensitive, and selective detection of E. coli in aqueous matrices.Ciencias Exactas y Naturales17 papplication/pdfhttps://doi.org/10.3390/molecules26092559https://repositorio.udes.edu.co/handle/001/6167engSulka, Grzegorz D.Bogdanowicz, RobertSuiza172559126Ropero-Vega, J.L.; Redondo-Ortega, J.F.; Galvis-Curubo, Y.J.; Rondón-Villarreal, P.; Flórez-Castillo, J.M. A Bioinspired Peptide in TIR Protein as Recognition Molecule on Electrochemical Biosensors for the Detection of E. coli O157:H7 in an Aqueous Matrix. Molecules 2021, 26, 2559. https:// doi.org/10.3390/molecules26092559ScopusMoleculesCopyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerlandinfo:eu-repo/semantics/openAccessAtribución 4.0 Internacional (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2https://www.mdpi.com/1420-3049/26/9/2559PathogenPEPTIR-1.0Screen-printed electrodesGold nanoparticlesElectrodepositionLimit of detectionSelectivityA bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157:H7 in an aqueous matrixArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Todas las AudienciasPublicationORIGINALA bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157H7 in an aqueous matrix.pdfA bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157H7 in an aqueous matrix.pdfapplication/pdf221772https://repositorio.udes.edu.co/bitstreams/bb2f4893-63e4-462f-a283-388a764d7c8b/download81465add811d7c633b4c1fcb2e8b38f4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/fdab6905-be37-4454-9eb8-7a353c8fbf7e/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTA bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157H7 in an aqueous matrix.pdf.txtA bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157H7 in an aqueous matrix.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/8aa59e4f-bb8e-49a9-86a5-29c365f03063/download5dbe86c1111d64f45ba435df98fdc825MD53THUMBNAILA bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157H7 in an aqueous matrix.pdf.jpgA bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157H7 in an aqueous matrix.pdf.jpgGenerated Thumbnailimage/jpeg12335https://repositorio.udes.edu.co/bitstreams/1029d3bd-fa2e-48fc-8d6e-c2bb76b6ad55/download603cf56ff423896b3e2c23cd017bbb24MD54001/6167oai:repositorio.udes.edu.co:001/61672023-10-11 09:04:17.718https://creativecommons.org/licenses/by/4.0/Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerlandhttps://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |