DNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis
10 p.
- Autores:
-
Pinzón Reyes, Efraín-Hernando
Sierra, Daniel A.
Suárez Barrera, Miguel Orlando
Orduz, Sergio
Florez, Alvaro M.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2017
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/3207
- Acceso en línea:
- https://repositorio.udes.edu.co/handle/001/3207
- Palabra clave:
- Bacillus thuringiensis
Cry toxins
DNA shuffling
DNA secondary structures in silico modeling
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Santander, 2017
| id |
RUDES2_43c2242f52838858aff77b30ddc4eeca |
|---|---|
| oai_identifier_str |
oai:repositorio.udes.edu.co:001/3207 |
| network_acronym_str |
RUDES2 |
| network_name_str |
Repositorio Universidad de Santander |
| repository_id_str |
|
| dc.title.eng.fl_str_mv |
DNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis |
| title |
DNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis |
| spellingShingle |
DNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis Bacillus thuringiensis Cry toxins DNA shuffling DNA secondary structures in silico modeling |
| title_short |
DNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis |
| title_full |
DNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis |
| title_fullStr |
DNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis |
| title_full_unstemmed |
DNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis |
| title_sort |
DNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis |
| dc.creator.fl_str_mv |
Pinzón Reyes, Efraín-Hernando Sierra, Daniel A. Suárez Barrera, Miguel Orlando Orduz, Sergio Florez, Alvaro M. |
| dc.contributor.author.spa.fl_str_mv |
Pinzón Reyes, Efraín-Hernando Sierra, Daniel A. Suárez Barrera, Miguel Orlando Orduz, Sergio Florez, Alvaro M. |
| dc.subject.proposal.eng.fl_str_mv |
Bacillus thuringiensis Cry toxins DNA shuffling DNA secondary structures in silico modeling |
| topic |
Bacillus thuringiensis Cry toxins DNA shuffling DNA secondary structures in silico modeling |
| description |
10 p. |
| publishDate |
2017 |
| dc.date.issued.spa.fl_str_mv |
2017-12 |
| dc.date.accessioned.spa.fl_str_mv |
2019-06-26T13:11:52Z |
| dc.date.available.spa.fl_str_mv |
2019-06-26T13:11:52Z |
| dc.type.spa.fl_str_mv |
Artículo de revista |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
http://purl.org/coar/resource_type/c_6501 |
| status_str |
publishedVersion |
| dc.identifier.doi.spa.fl_str_mv |
10.1186/s13628-017-0036-7 |
| dc.identifier.issn.spa.fl_str_mv |
2046-1682 |
| dc.identifier.uri.spa.fl_str_mv |
https://repositorio.udes.edu.co/handle/001/3207 |
| identifier_str_mv |
10.1186/s13628-017-0036-7 2046-1682 |
| url |
https://repositorio.udes.edu.co/handle/001/3207 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.eng.fl_str_mv |
BMC Biophysics |
| dc.rights.spa.fl_str_mv |
Derechos Reservados - Universidad de Santander, 2017 |
| dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.creativecommons.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0/ |
| rights_invalid_str_mv |
Derechos Reservados - Universidad de Santander, 2017 Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.source.eng.fl_str_mv |
https://link.springer.com/content/pdf/10.1186%2Fs13628-017-0036-7.pdf |
| institution |
Universidad de Santander |
| bitstream.url.fl_str_mv |
https://repositorio.udes.edu.co/bitstreams/d4d5a7e1-89fd-49d3-a4a9-59367e9af4d2/download https://repositorio.udes.edu.co/bitstreams/4114bc09-ac58-41a0-8e0a-4f9101703214/download https://repositorio.udes.edu.co/bitstreams/16fc930b-78bd-4b8c-8c5a-16cece41b43c/download https://repositorio.udes.edu.co/bitstreams/f01f7d8a-1954-433c-9e29-95721397e3c3/download https://repositorio.udes.edu.co/bitstreams/b35ac684-d30c-4318-a818-4cb28effde11/download https://repositorio.udes.edu.co/bitstreams/d28bfc44-f53e-4ca9-b53f-041beb037d41/download |
| bitstream.checksum.fl_str_mv |
d93ca08f7fe351b4d1988e31971afaa7 56781cfa00709f54f4195a96b8dd13f7 38d94cf55aa1bf2dac1a736ac45c881c 395fd596223259ca54e781c1cf1f75f7 42700f0fd2ccb2330029010f321aa02f 5dbe86c1111d64f45ba435df98fdc825 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Universidad de Santander |
| repository.mail.fl_str_mv |
soporte@metabiblioteca.com |
| _version_ |
1814158825526132736 |
| spelling |
Pinzón Reyes, Efraín-Hernando82c312e2-e21a-425e-a04b-cc23e78eb6ed-1Sierra, Daniel A.d5ca3fe3-64f5-41df-8eec-af0b63dfb9bc-1Suárez Barrera, Miguel Orlando99b4d9f0-b967-4877-9973-dbb3381a16ff-1Orduz, Sergiod94079f7-48c0-4449-85b1-539f320c34c9-1Florez, Alvaro M.03b37924-311e-4ca6-bae7-fb60528ecb1c-12019-06-26T13:11:52Z2019-06-26T13:11:52Z2017-1210 p.Background The Cry toxins, or δ-endotoxins, are a diverse group of proteins produced by Bacillus thuringiensis. While DNA secondary structures are biologically relevant, it is unknown if such structures are formed in regions encoding conserved domains of Cry toxins under shuffling conditions. We analyzed 5 holotypes that encode Cry toxins and that grouped into 4 clusters according to their phylogenetic closeness. The mean number of DNA secondary structures that formed and the mean Gibbs free energy (ΔG¯¯¯¯¯¯¯¯) were determined by an in silico analysis using different experimental DNA shuffling scenarios. In terms of spontaneity, shuffling efficiency was directly proportional to the formation of secondary structures but inversely proportional to ∆G. Results The results showed a shared thermodynamic pattern for each cluster and relationships among sequences that are phylogenetically close at the protein level. The regions of the cry11Aa, Ba and Bb genes that encode domain I showed more spontaneity and thus a greater tendency to form secondary structures (<∆G). In the region of domain III; this tendency was lower (>∆G) in the cry11Ba and Bb genes. Proteins that are phylogenetically closer to Cry11Ba and Cry11Bb, such as Cry2Aa and Cry18Aa, maintained the same thermodynamic pattern. More distant proteins, such as Cry1Aa, Cry1Ab, Cry30Aa and Cry30Ca, featured different thermodynamic patterns in their DNA. Conclusion These results suggest the presence of thermodynamic variations associated to the formation of secondary structures and an evolutionary relationship with regions that encode highly conserved domains in Cry proteins. The findings of this study may have a role in the in silico design of cry gene assembly by DNA shuffling techniques.application/pdf10.1186/s13628-017-0036-72046-1682https://repositorio.udes.edu.co/handle/001/3207engBMC BiophysicsDerechos Reservados - Universidad de Santander, 2017info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://link.springer.com/content/pdf/10.1186%2Fs13628-017-0036-7.pdfBacillus thuringiensisCry toxinsDNA shufflingDNA secondary structures in silico modelingDNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensisArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85PublicationTHUMBNAILDNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of.pdf.jpgDNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of.pdf.jpgGenerated Thumbnailimage/jpeg1780https://repositorio.udes.edu.co/bitstreams/d4d5a7e1-89fd-49d3-a4a9-59367e9af4d2/downloadd93ca08f7fe351b4d1988e31971afaa7MD54DNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis.pdf.jpgDNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis.pdf.jpgGenerated Thumbnailimage/jpeg12657https://repositorio.udes.edu.co/bitstreams/4114bc09-ac58-41a0-8e0a-4f9101703214/download56781cfa00709f54f4195a96b8dd13f7MD57LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/16fc930b-78bd-4b8c-8c5a-16cece41b43c/download38d94cf55aa1bf2dac1a736ac45c881cMD52ORIGINALDNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis.pdfDNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis.pdfapplication/pdf343151https://repositorio.udes.edu.co/bitstreams/f01f7d8a-1954-433c-9e29-95721397e3c3/download395fd596223259ca54e781c1cf1f75f7MD55TEXTDNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of.pdf.txtDNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of.pdf.txtExtracted texttext/plain47520https://repositorio.udes.edu.co/bitstreams/b35ac684-d30c-4318-a818-4cb28effde11/download42700f0fd2ccb2330029010f321aa02fMD53DNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis.pdf.txtDNA secondary structure formation by DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/d28bfc44-f53e-4ca9-b53f-041beb037d41/download5dbe86c1111d64f45ba435df98fdc825MD56001/3207oai:repositorio.udes.edu.co:001/32072023-10-11 11:08:02.893https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Santander, 2017https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |