Enteroinvasive Escherichia coli O96:H19 is an Emergent Biofilm-Forming Pathogen
Digital
- Autores:
-
Iqbal, Junaid
Malviya, Niharika
Gaddy, Jennifer A.
Zhang, Chengxian
Seier, Andrew J.
Haley, Kathryn P.
Doster, Ryan S.
Farfan-Garcia, Ana Elvira
Gómez-Duarte, Oscar G.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2022
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/7334
- Acceso en línea:
- https://doi.org/10.1128/jb.00562-21
https://repositorio.udes.edu.co/handle/001/7334
- Palabra clave:
- Rights
- closedAccess
- License
- Copyright © 2022 The Authors, American Society for Microbiology
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Enteroinvasive Escherichia coli O96:H19 is an Emergent Biofilm-Forming Pathogen |
| title |
Enteroinvasive Escherichia coli O96:H19 is an Emergent Biofilm-Forming Pathogen |
| spellingShingle |
Enteroinvasive Escherichia coli O96:H19 is an Emergent Biofilm-Forming Pathogen |
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Enteroinvasive Escherichia coli O96:H19 is an Emergent Biofilm-Forming Pathogen |
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Enteroinvasive Escherichia coli O96:H19 is an Emergent Biofilm-Forming Pathogen |
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Enteroinvasive Escherichia coli O96:H19 is an Emergent Biofilm-Forming Pathogen |
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Enteroinvasive Escherichia coli O96:H19 is an Emergent Biofilm-Forming Pathogen |
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Enteroinvasive Escherichia coli O96:H19 is an Emergent Biofilm-Forming Pathogen |
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Iqbal, Junaid Malviya, Niharika Gaddy, Jennifer A. Zhang, Chengxian Seier, Andrew J. Haley, Kathryn P. Doster, Ryan S. Farfan-Garcia, Ana Elvira Gómez-Duarte, Oscar G. |
| dc.contributor.author.none.fl_str_mv |
Iqbal, Junaid Malviya, Niharika Gaddy, Jennifer A. Zhang, Chengxian Seier, Andrew J. Haley, Kathryn P. Doster, Ryan S. Farfan-Garcia, Ana Elvira Gómez-Duarte, Oscar G. |
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CliniUDES |
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2022 |
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2022-08-03T16:35:12Z |
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2022-08-03T16:35:12Z |
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2022-03-28 |
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https://doi.org/10.1128/jb.00562-21 |
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0021-919 |
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https://repositorio.udes.edu.co/handle/001/7334 |
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1098-5530 0021-919 |
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https://doi.org/10.1128/jb.00562-21 https://repositorio.udes.edu.co/handle/001/7334 |
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eng |
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eng |
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4 |
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204 |
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Scopus |
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Journal of Bacteriology |
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Copyright © 2022 The Authors, American Society for Microbiology |
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Copyright © 2022 The Authors, American Society for Microbiology Atribución 4.0 Internacional (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/ http://purl.org/coar/access_right/c_14cb |
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USA |
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Universidad de Santander |
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Iqbal, Junaidc7776124-d30d-4152-ac43-a935d08d9770-1Malviya, Niharika34d70577-f721-4be0-b7e5-0a5e300fa506-1Gaddy, Jennifer A.94fab554-ac12-43dd-8a46-fd6b2e6b0457-1Zhang, Chengxiana524736f-1323-48cb-bc34-f0769a860493-1Seier, Andrew J.8fae9a69-fab2-49e4-9d4b-96b9d9712dcb-1Haley, Kathryn P.de4aff6a-41f6-48d2-8907-48edf94a0b83-1Doster, Ryan S.7d9c83d6-c8a5-4df5-b9e2-5622561cdddd-1Farfan-Garcia, Ana Elvira58566add-ecc0-4b99-a208-6bc17e599fc8-1Gómez-Duarte, Oscar G.a2dbbaaf-ccc7-43db-96d4-f7d083ad619e-1CliniUDES2022-08-03T16:35:12Z2022-08-03T16:35:12Z2022-03-28DigitalEnteroinvasive Escherichia coli (EIEC) is a diarrheagenic E. coli pathotype carrying a virulence plasmid that encodes a type III secretion system (TTSS) directly implicated in bacterial cell invasion. Since 2012, EIEC serotype O96:H19 has been recognized in Europe, Colombia, and most recently Uruguay. In addition to the invasion phenotype, the strains isolated from Colombian children with moderate-to-severe gastroenteritis had a strong biofilm formation phenotype, and as a result, they are referred to as biofilm-forming enteroinvasive E. coli (BF-EIEC). The objective of this study was to characterize the biofilm formation phenotype of the BF-EIEC O96:H19 strain 52.1 isolated from a child with moderate-to-severe gastroenteritis in Colombia. Random mutagenesis using Tn5 transposons identified 100 mutants unable to form biofilm; 20 of those had mutations within the pgaABCD operon. Site-directed mutagenesis of pgaB and pgaC confirmed the importance of these genes in N-acetylglucosamine-mediated biofilm formation. Both biofilm formation and TTSS-mediated host cell invasion were associated with host cell damage on the basis of cytotoxic assays comparing the wild type, invasion gene mutants, and biofilm formation mutants. Multilocus sequence typing-based phylogenetic analysis showed that BF-EIEC strain 52.1 does not cluster with classic EIEC serotype strains. Instead, BF-EIEC strain 52.1 clusters with EIEC serotype O96:H19 strains described in Europe and Uruguay. In conclusion, BF-EIEC O96:H19, an emerging pathogen associated with moderate-to-severe acute gastroenteritis in children under 5 years of age in Colombia, invades cells and has a strong biofilm formation capability. Both phenotypes are independently associated with in vitro cell cytotoxicity, and they may explain, at least in part, the higher disease severity reported in Europe and Latin America. IMPORTANCE Enteroinvasive Escherichia coli (EIEC), a close relative of Shigella, is implicated in dysenteric diarrhea. EIEC pathogenicity involves cell invasion mediated by effector proteins delivered by a type III secretion system (TTSS) that disrupt the cell cytoskeleton. These proteins and the VirF global regulator are encoded by a large (>200 kb) invasion plasmid (pINV). This study reports an emergent EIEC possessing a cell invasion phenotype and a strong polysaccharide matrix-mediated biofilm formation phenotype. Both phenotypes contribute to host cell cytotoxicity in vitro and may contribute to the severe disease reported among children and adults in Europe and Latin America.Ciencias Médicas y de la Saludapplication/pdf1098-5530https://doi.org/10.1128/jb.00562-210021-919https://repositorio.udes.edu.co/handle/001/7334engUSA4204ScopusJournal of BacteriologyCopyright © 2022 The Authors, American Society for Microbiologyinfo:eu-repo/semantics/closedAccessAtribución 4.0 Internacional (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_14cbhttps://journals.asm.org/doi/10.1128/jb.00562-21Enteroinvasive Escherichia coli O96:H19 is an Emergent Biofilm-Forming PathogenArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Todas las AudienciasPublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/86c80fba-df5a-4ab6-bed7-3fda20d5a8c9/download38d94cf55aa1bf2dac1a736ac45c881cMD52ORIGINALEnteroinvasive Escherichia coli O96.H19 is an Emergent Biofilm-Forming Pathogen.pdfEnteroinvasive Escherichia coli O96.H19 is an Emergent Biofilm-Forming Pathogen.pdfapplication/pdf233895https://repositorio.udes.edu.co/bitstreams/a5c9e380-7bdd-4090-9e5e-66b7012d5619/downloadde8573f9080f71365696aa6288085373MD51TEXTEnteroinvasive Escherichia coli O96.H19 is an Emergent Biofilm-Forming Pathogen.pdf.txtEnteroinvasive Escherichia coli O96.H19 is an Emergent Biofilm-Forming Pathogen.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/bf65d3e0-4e9b-43a5-bccc-b39e42119ed2/download5dbe86c1111d64f45ba435df98fdc825MD53THUMBNAILEnteroinvasive Escherichia coli O96.H19 is an Emergent Biofilm-Forming Pathogen.pdf.jpgEnteroinvasive Escherichia coli O96.H19 is an Emergent Biofilm-Forming Pathogen.pdf.jpgGenerated Thumbnailimage/jpeg6576https://repositorio.udes.edu.co/bitstreams/a448009c-61e8-44ae-8a58-0ff5c01eccc6/downloadc226320a1169d9f52066758e04b7d816MD54001/7334oai:repositorio.udes.edu.co:001/73342023-10-10 09:33:19.324https://creativecommons.org/licenses/by/4.0/Copyright © 2022 The Authors, American Society for Microbiologyhttps://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |