Functional Profile of CD8+ T-Cells in Response to HLA-A*02:01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia
Digital
- Autores:
-
Sanchez-Martinez, Alexandra
Acevedo-Saenz, Liliana
Alzate-Angel, Juan Carlos
Alvarez, Cristian M.
Guzman, Fanny
Roman, Tanya
Urcuqui-Inchima, Silvio
Cardona-Maya, Walter D.
Velilla, Paula Andrea
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2022
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/7370
- Acceso en línea:
- https://doi.org/10.3389/fimmu.2022.7939
https://repositorio.udes.edu.co/handle/001/7370
- Palabra clave:
- CD8+ T-cells
T-cell epitopes
HIV-1
HLA-A*02
Polyfunctionality
Class I HLA-peptide binding affinity
Gag-derived peptides
- Rights
- openAccess
- License
- © 2022 The Authors, Frontiers Media S.A. All rights reserved
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| dc.title.spa.fl_str_mv |
Functional Profile of CD8+ T-Cells in Response to HLA-A*02:01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia |
| title |
Functional Profile of CD8+ T-Cells in Response to HLA-A*02:01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia |
| spellingShingle |
Functional Profile of CD8+ T-Cells in Response to HLA-A*02:01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia CD8+ T-cells T-cell epitopes HIV-1 HLA-A*02 Polyfunctionality Class I HLA-peptide binding affinity Gag-derived peptides |
| title_short |
Functional Profile of CD8+ T-Cells in Response to HLA-A*02:01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia |
| title_full |
Functional Profile of CD8+ T-Cells in Response to HLA-A*02:01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia |
| title_fullStr |
Functional Profile of CD8+ T-Cells in Response to HLA-A*02:01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia |
| title_full_unstemmed |
Functional Profile of CD8+ T-Cells in Response to HLA-A*02:01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia |
| title_sort |
Functional Profile of CD8+ T-Cells in Response to HLA-A*02:01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia |
| dc.creator.fl_str_mv |
Sanchez-Martinez, Alexandra Acevedo-Saenz, Liliana Alzate-Angel, Juan Carlos Alvarez, Cristian M. Guzman, Fanny Roman, Tanya Urcuqui-Inchima, Silvio Cardona-Maya, Walter D. Velilla, Paula Andrea |
| dc.contributor.author.none.fl_str_mv |
Sanchez-Martinez, Alexandra Acevedo-Saenz, Liliana Alzate-Angel, Juan Carlos Alvarez, Cristian M. Guzman, Fanny Roman, Tanya Urcuqui-Inchima, Silvio Cardona-Maya, Walter D. Velilla, Paula Andrea |
| dc.contributor.researchgroup.spa.fl_str_mv |
Masira |
| dc.subject.proposal.eng.fl_str_mv |
CD8+ T-cells T-cell epitopes HIV-1 HLA-A*02 Polyfunctionality Class I HLA-peptide binding affinity Gag-derived peptides |
| topic |
CD8+ T-cells T-cell epitopes HIV-1 HLA-A*02 Polyfunctionality Class I HLA-peptide binding affinity Gag-derived peptides |
| description |
Digital |
| publishDate |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2022-08-09T22:01:40Z |
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2022-08-09T22:01:40Z |
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2022-03-22 |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://doi.org/10.3389/fimmu.2022.7939 |
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1664-3224 |
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1664-3224 |
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https://repositorio.udes.edu.co/handle/001/7370 |
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https://doi.org/10.3389/fimmu.2022.7939 https://repositorio.udes.edu.co/handle/001/7370 |
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1664-3224 |
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eng |
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eng |
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13 |
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Sa´ nchez-Mart´ınez A, Acevedo-Sa´ enz L, Alzate-A´ ngel JC, A´ lvarez CM, Guzma´n F, Roman T, Urcuqui-Inchima S, Cardona-Maya WD and Velilla PA (2022) Functional Profile of CD8+ T-Cells in Response to HLAA02: 01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medell´ın, Colombia. Front. Immunol. 13:793982. doi: 10.3389/fimmu.2022.793982 |
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Scopus |
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Frontiers in Immunology |
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© 2022 The Authors, Frontiers Media S.A. All rights reserved |
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Atribución 4.0 Internacional (CC BY 4.0) |
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© 2022 The Authors, Frontiers Media S.A. All rights reserved Atribución 4.0 Internacional (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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14 p |
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application/pdf |
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Suiza |
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https://www.frontiersin.org/articles/10.3389/fimmu.2022.793982/full |
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Universidad de Santander |
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Sanchez-Martinez, Alexandra86e7cfcc-99a1-40f9-8e79-0d08d132f9e2-1Acevedo-Saenz, Lilianaca733848-621c-4a0f-b980-71fa4e86eb34-1Alzate-Angel, Juan Carlos34627720-a277-4862-8020-e5fbbb689d0e-1Alvarez, Cristian M.37322fd2-368b-4034-a2a4-e3c5d5e12561-1Guzman, Fannye2162a90-5602-4223-8aa6-e2e1564ba308-1Roman, Tanyaf41740a6-b092-4bb8-a001-0f08918ff68c-1Urcuqui-Inchima, Silvio5f2d56f5-fcb1-4f6e-9ca7-d568fa5701c1-1Cardona-Maya, Walter D.64fe92bd-a7fa-4ab3-92fe-4dea291196a2-1Velilla, Paula Andreabae6ccca-6303-4d9c-8928-ffb086623802-1Masira2022-08-09T22:01:40Z2022-08-09T22:01:40Z2022-03-22DigitalCD8+ T-cells play a crucial role in the control of HIV replication. HIV-specific CD8+ T-cell responses rapidly expand since the acute phase of the infection, and it has been observed that HIV controllers harbor CD8+ T-cells with potent anti-HIV capacity. The development of CD8+ T-cell-based vaccine against HIV-1 has focused on searching for immunodominant epitopes. However, the strong immune pressure of CD8+ T-cells causes the selection of viral variants with mutations in immunodominant epitopes. Since HIV-1 mutations are selected under the context of a specific HLA-I, the circulation of viral variants with these mutations is highly predictable based on the most prevalent HLA-I within a population. We previously demonstrated the adaptation of circulating strains of HIV-1 to the HLA-A*02 molecule by identifying mutations under positive selection located in GC9 and SL9 epitopes derived from the Gag protein. Also, we used an in silico prediction approach and evaluated whether the mutations found had a higher or lower affinity to the HLA-A*02. Although this strategy allowed predicting the interaction between mutated peptides and HLA-I, the functional response of CD8+ T-cells that these peptides induce is unknown. In the present work, peripheral blood mononuclear cells from 12 HIV-1+ HLA-A*02:01+ individuals were stimulated with the mutated and wild-type peptides derived from the GC9 and SL9 epitopes. The functional profile of CD8+ T-cells was evaluated using flow cytometry, and the frequency of subpopulations was determined according to their number of functions and the polyfunctionality index. The results suggest that the quality of the response (polyfunctionality) could be associated with the binding affinity of the peptide to the HLA molecule, and the functional profile of specific CD8+ T-cells to mutated epitopes in individuals under cART is maintained.Ciencias Médicas y de la Salud14 papplication/pdfhttps://doi.org/10.3389/fimmu.2022.79391664-32241664-3224https://repositorio.udes.edu.co/handle/001/7370engSuiza13Sa´ nchez-Mart´ınez A, Acevedo-Sa´ enz L, Alzate-A´ ngel JC, A´ lvarez CM, Guzma´n F, Roman T, Urcuqui-Inchima S, Cardona-Maya WD and Velilla PA (2022) Functional Profile of CD8+ T-Cells in Response to HLAA02: 01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medell´ın, Colombia. Front. Immunol. 13:793982. doi: 10.3389/fimmu.2022.793982ScopusFrontiers in Immunology© 2022 The Authors, Frontiers Media S.A. All rights reservedinfo:eu-repo/semantics/openAccessAtribución 4.0 Internacional (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2https://www.frontiersin.org/articles/10.3389/fimmu.2022.793982/fullCD8+ T-cellsT-cell epitopesHIV-1HLA-A*02PolyfunctionalityClass I HLA-peptide binding affinityGag-derived peptidesFunctional Profile of CD8+ T-Cells in Response to HLA-A*02:01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, ColombiaArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Todas las AudienciasPublicationORIGINALFunctional Profile of CD8+ T-Cells in Response to HLA-A 02.01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia.pdfFunctional Profile of CD8+ T-Cells in Response to HLA-A 02.01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia.pdfapplication/pdf229882https://repositorio.udes.edu.co/bitstreams/7b992a9f-6107-42d6-bdbe-b0390cc35b27/download7dea4c1827254c60f988dec4dafa1f51MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/f18a14e3-2e39-4350-ab04-58e7daf7fb2e/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTFunctional Profile of CD8+ T-Cells in Response to HLA-A 02.01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia.pdf.txtFunctional Profile of CD8+ T-Cells in Response to HLA-A 02.01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/db2d4f87-db0e-455e-9ec2-4afd1cbe816c/download5dbe86c1111d64f45ba435df98fdc825MD53THUMBNAILFunctional Profile of CD8+ T-Cells in Response to HLA-A 02.01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia.pdf.jpgFunctional Profile of CD8+ T-Cells in Response to HLA-A 02.01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia.pdf.jpgGenerated Thumbnailimage/jpeg11587https://repositorio.udes.edu.co/bitstreams/40f314b1-3934-46bb-a3d0-7f80e7d05862/download5aabd3e33407d6b1f82db8cee4ab9507MD54001/7370oai:repositorio.udes.edu.co:001/73702023-10-09 18:03:38.608https://creativecommons.org/licenses/by/4.0/© 2022 The Authors, Frontiers Media S.A. All rights reservedhttps://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |