A new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method
14 p.
- Autores:
-
García Sánchez, Liliana Torcoroma
Cristancho, Laura Maritza
Vera, Erika Patricia
Begambre, Oscar
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2015
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/3499
- Acceso en línea:
- https://repositorio.udes.edu.co/handle/001/3499
- Palabra clave:
- Primer Design
Multiplex-PCR
Urinary Tract Infections
Uropathogens
Hybrid Particle Swarm Optimization
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Santander, 2015
| id |
RUDES2_2feb7f4375322d1de32497d7780c2b49 |
|---|---|
| oai_identifier_str |
oai:repositorio.udes.edu.co:001/3499 |
| network_acronym_str |
RUDES2 |
| network_name_str |
Repositorio Universidad de Santander |
| repository_id_str |
|
| dc.title.eng.fl_str_mv |
A new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method |
| title |
A new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method |
| spellingShingle |
A new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method Primer Design Multiplex-PCR Urinary Tract Infections Uropathogens Hybrid Particle Swarm Optimization |
| title_short |
A new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method |
| title_full |
A new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method |
| title_fullStr |
A new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method |
| title_full_unstemmed |
A new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method |
| title_sort |
A new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method |
| dc.creator.fl_str_mv |
García Sánchez, Liliana Torcoroma Cristancho, Laura Maritza Vera, Erika Patricia Begambre, Oscar |
| dc.contributor.author.spa.fl_str_mv |
García Sánchez, Liliana Torcoroma Cristancho, Laura Maritza Vera, Erika Patricia Begambre, Oscar |
| dc.subject.proposal.eng.fl_str_mv |
Primer Design Multiplex-PCR Urinary Tract Infections Uropathogens Hybrid Particle Swarm Optimization |
| topic |
Primer Design Multiplex-PCR Urinary Tract Infections Uropathogens Hybrid Particle Swarm Optimization |
| description |
14 p. |
| publishDate |
2015 |
| dc.date.issued.spa.fl_str_mv |
2015-06 |
| dc.date.accessioned.spa.fl_str_mv |
2019-07-30T19:44:25Z |
| dc.date.available.spa.fl_str_mv |
2019-07-30T19:44:25Z |
| dc.type.spa.fl_str_mv |
Artículo de revista |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
http://purl.org/coar/resource_type/c_6501 |
| status_str |
publishedVersion |
| dc.identifier.doi.spa.fl_str_mv |
10.4014/jmb.1406.06079 |
| dc.identifier.issn.spa.fl_str_mv |
1738-8872 1017-7825 |
| dc.identifier.uri.spa.fl_str_mv |
https://repositorio.udes.edu.co/handle/001/3499 |
| identifier_str_mv |
10.4014/jmb.1406.06079 1738-8872 1017-7825 |
| url |
https://repositorio.udes.edu.co/handle/001/3499 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.eng.fl_str_mv |
Journal of Microbiology and Biotechnology |
| dc.rights.spa.fl_str_mv |
Derechos Reservados - Universidad de Santander, 2015 |
| dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.creativecommons.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0/ |
| rights_invalid_str_mv |
Derechos Reservados - Universidad de Santander, 2015 Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.source.eng.fl_str_mv |
http://www.jmb.or.kr/submission/Journal/025/JMB025-10-19_FDOC_1.pdf |
| institution |
Universidad de Santander |
| bitstream.url.fl_str_mv |
https://repositorio.udes.edu.co/bitstreams/5ff3842c-cefb-46ce-be0e-4f5cc9a71194/download https://repositorio.udes.edu.co/bitstreams/a8963247-d65a-43c9-b920-baf37aad14f8/download https://repositorio.udes.edu.co/bitstreams/93200aa3-bc88-48f8-9c1d-4e40fa2407a7/download https://repositorio.udes.edu.co/bitstreams/8278c964-f0f3-4cda-9b16-b52ae1678ef1/download https://repositorio.udes.edu.co/bitstreams/fe1c6565-81cb-4165-af52-fcf4d0c2ed63/download https://repositorio.udes.edu.co/bitstreams/677b29a8-9f7e-48cd-9957-6b3f75ed79f8/download |
| bitstream.checksum.fl_str_mv |
a535e94ff479af1494542fba4f347b2e ef9f70dc30e52992bdfe3ec396516dc3 9b0e2a59fe71d65a5a9d003572430d90 5dbe86c1111d64f45ba435df98fdc825 38d94cf55aa1bf2dac1a736ac45c881c 1e23c19ca57c7691dccd265b70f8cf3b |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Universidad de Santander |
| repository.mail.fl_str_mv |
soporte@metabiblioteca.com |
| _version_ |
1814159049060515840 |
| spelling |
García Sánchez, Liliana Torcoroma5b6d6a1a-21c5-4db6-8dc3-3f5c61c9190f-1Cristancho, Laura Maritza7cb6fdc6-deff-487b-b8db-8c1e5d8d5a16-1Vera, Erika Patriciac72c89e1-a675-4e4f-b502-8436ba5ca6cd-1Begambre, Oscaradb6aaaa-a2ee-4b74-9be2-e0cb6b12ad7a-12019-07-30T19:44:25Z2019-07-30T19:44:25Z2015-0614 p.This work describes a new strategy for optimal design of Multiplex-PCR primer sequences. The process is based on the Particle Swarm Optimization-Simplex algorithm (Mult-PSOS). Diverging from previous solutions centered on heuristic tools, the Mult-PSOS is selfconfigured because it does not require the definition of the algorithm’s initial search parameters. The successful performance of this method was validated in vitro using Multiplex-PCR assays. For this validation, seven gene sequences of the most prevalent bacteria implicated in urinary tract infections were taken as DNA targets. The in vitro tests confirmed the good performance of the Mult-PSOS, with respect to infectious disease diagnosis, in the rapid and efficient selection of the optimal oligonucleotide sequences for Multiplex-PCRs. The predicted sequences allowed the adequate amplification of all amplicons in a single step (with the correct amount of DNA template and primers), reducing significantly the need for trial and error experiments. In addition, owing to its independence from the initial selection of the heuristic constants, the Mult-PSOS can be employed by non-expert users in computational techniques or in primer design problems.application/pdf10.4014/jmb.1406.060791738-88721017-7825https://repositorio.udes.edu.co/handle/001/3499engJournal of Microbiology and BiotechnologyDerechos Reservados - Universidad de Santander, 2015info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2http://www.jmb.or.kr/submission/Journal/025/JMB025-10-19_FDOC_1.pdfPrimer DesignMultiplex-PCRUrinary Tract InfectionsUropathogensHybrid Particle Swarm OptimizationA new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation methodArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85PublicationTHUMBNAILA new multiplex-PCR for urinary tract pathogen detection using primer design based on an evolutionary computation method.pdf.jpgA new multiplex-PCR for urinary tract pathogen detection using primer design based on an evolutionary computation method.pdf.jpgGenerated Thumbnailimage/jpeg1653https://repositorio.udes.edu.co/bitstreams/5ff3842c-cefb-46ce-be0e-4f5cc9a71194/downloada535e94ff479af1494542fba4f347b2eMD54A new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method.pdf.jpgA new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method.pdf.jpgGenerated Thumbnailimage/jpeg13246https://repositorio.udes.edu.co/bitstreams/a8963247-d65a-43c9-b920-baf37aad14f8/downloadef9f70dc30e52992bdfe3ec396516dc3MD57TEXTA new multiplex-PCR for urinary tract pathogen detection using primer design based on an evolutionary computation method.pdf.txtA new multiplex-PCR for urinary tract pathogen detection using primer design based on an evolutionary computation method.pdf.txtExtracted texttext/plain57053https://repositorio.udes.edu.co/bitstreams/93200aa3-bc88-48f8-9c1d-4e40fa2407a7/download9b0e2a59fe71d65a5a9d003572430d90MD53A new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method.pdf.txtA new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/8278c964-f0f3-4cda-9b16-b52ae1678ef1/download5dbe86c1111d64f45ba435df98fdc825MD56LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/fe1c6565-81cb-4165-af52-fcf4d0c2ed63/download38d94cf55aa1bf2dac1a736ac45c881cMD52ORIGINALA new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method.pdfA new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method.pdfapplication/pdf291562https://repositorio.udes.edu.co/bitstreams/677b29a8-9f7e-48cd-9957-6b3f75ed79f8/download1e23c19ca57c7691dccd265b70f8cf3bMD55001/3499oai:repositorio.udes.edu.co:001/34992023-10-11 11:04:06.979https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Santander, 2015https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |