Development of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas
Digital
- Autores:
-
Patiño, Luz H.
Castillo-Castañeda, Adriana C.
Muñoz, Marina
Jaimes, Jesus E.
Luna-Niño, Nicolas
Hernández, Carolina
Ayala, Martha S.
Fuya, Patricia
Mendez, Claudia
Hernández-Pereira, Carlos E.
Delgado, Lourdes
Sandoval-Ramírez, Claudia Magaly
Urbano, Plutarco
Paniz-Mondolfi, Alberto
Ramírez, Juan David
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2021
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/6171
- Acceso en línea:
- https://doi.org/10.1128/Spectrum.00652-21
https://repositorio.udes.edu.co/handle/001/6171
- Palabra clave:
- Amplicon-based NGS
Leishmania
NGS
Species
- Rights
- openAccess
- License
- © 2021 Patiño et al.
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Development of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas |
| title |
Development of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas |
| spellingShingle |
Development of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas Amplicon-based NGS Leishmania NGS Species |
| title_short |
Development of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas |
| title_full |
Development of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas |
| title_fullStr |
Development of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas |
| title_full_unstemmed |
Development of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas |
| title_sort |
Development of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas |
| dc.creator.fl_str_mv |
Patiño, Luz H. Castillo-Castañeda, Adriana C. Muñoz, Marina Jaimes, Jesus E. Luna-Niño, Nicolas Hernández, Carolina Ayala, Martha S. Fuya, Patricia Mendez, Claudia Hernández-Pereira, Carlos E. Delgado, Lourdes Sandoval-Ramírez, Claudia Magaly Urbano, Plutarco Paniz-Mondolfi, Alberto Ramírez, Juan David |
| dc.contributor.author.none.fl_str_mv |
Patiño, Luz H. Castillo-Castañeda, Adriana C. Muñoz, Marina Jaimes, Jesus E. Luna-Niño, Nicolas Hernández, Carolina Ayala, Martha S. Fuya, Patricia Mendez, Claudia Hernández-Pereira, Carlos E. Delgado, Lourdes Sandoval-Ramírez, Claudia Magaly Urbano, Plutarco Paniz-Mondolfi, Alberto Ramírez, Juan David |
| dc.contributor.researchgroup.spa.fl_str_mv |
Cibas |
| dc.subject.proposal.eng.fl_str_mv |
Amplicon-based NGS Leishmania NGS Species |
| topic |
Amplicon-based NGS Leishmania NGS Species |
| description |
Digital |
| publishDate |
2021 |
| dc.date.issued.none.fl_str_mv |
2021-10-13 |
| dc.date.accessioned.none.fl_str_mv |
2022-02-25T21:38:00Z |
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2022-02-25T21:38:00Z |
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Artículo de revista |
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https://doi.org/10.1128/Spectrum.00652-21 |
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https://repositorio.udes.edu.co/handle/001/6171 |
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https://doi.org/10.1128/Spectrum.00652-21 https://repositorio.udes.edu.co/handle/001/6171 |
| dc.language.iso.spa.fl_str_mv |
eng |
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eng |
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15 |
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Patiño LH, Castillo-Castañeda AC, Muñoz M, Jaimes JE, Luna-Niño N, Hernández C, Ayala MS, Fuya P, Mendez C, HernándezPereira CE, Delgado L, Sandoval-Ramírez CM, Urbano P, Paniz-Mondolfi A, Ramírez JD. 2021. Development of an amplicon-based nextgeneration sequencing protocol to identify Leishmania species and other trypanosomatids in Leishmaniasis endemic areas. Microbiol Spectr 9:e00652-21. https://doi.org/10.1128/ Spectrum.00652-21. |
| dc.relation.indexed.spa.fl_str_mv |
Scopus |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Microbiology Spectrum |
| dc.rights.spa.fl_str_mv |
© 2021 Patiño et al. |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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https://creativecommons.org/licenses/by-nc/4.0/ |
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© 2021 Patiño et al. Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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15 p |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Downing Tim, Dublin City University |
| dc.publisher.place.spa.fl_str_mv |
USA |
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https://journals.asm.org/doi/pdf/10.1128/Spectrum.00652-21 |
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Universidad de Santander |
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Patiño, Luz H.c68fb087-7ad3-4fbb-9ae5-65f4f15ca1d6-1Castillo-Castañeda, Adriana C.69a787e7-0738-4739-a78f-f797ba458ac9-1Muñoz, Marina2b2d8c78-7aab-49e0-98b0-511a68682cf0-1Jaimes, Jesus E.69a8fa50-69ff-4a6a-93f0-5d92a4474f5f-1Luna-Niño, Nicolasf88d4dcb-b566-423b-9a7a-343f46ac924a-1Hernández, Carolinabfe9a7b2-d2a9-4e1f-9e74-4c5b5513dd2d-1Ayala, Martha S.6a7c1b65-b60c-4864-8827-62bfa6eca109-1Fuya, Patriciad98bd1a6-3d92-43c6-b59d-ec27687948c3-1Mendez, Claudiacba56416-d809-41ce-bf32-552fdfea9a52-1Hernández-Pereira, Carlos E.5c5721f2-2b28-40d9-a2cf-5835f6b98c6c-1Delgado, Lourdes138f97c7-98cc-4d38-99fc-aa7ef5d66173-1Sandoval-Ramírez, Claudia Magaly409dd559-5593-4d5c-83e0-59863bf83d64-1Urbano, Plutarco8ca6b953-93da-44be-80bb-842fa5df012a-1Paniz-Mondolfi, Albertoda8f3f19-9756-4c58-ad1e-fb4b1aa9cd83-1Ramírez, Juan David6caf7c56-3a35-4fed-9d77-f176391b24ad-1Cibas2022-02-25T21:38:00Z2022-02-25T21:38:00Z2021-10-13DigitalTrypanosomatid infections are an important public health threat affecting many low-income countries across the tropics, particularly in the Americas. Trypanosomatids can infect many vertebrate, invertebrate, and plant species and play an important role as human pathogens. Among these clinically relevant pathogens are species from the genera Leishmania and Trypanosoma. Mixed trypanosomatid infections remain a largely unexplored phenomenon. Herein, we describe the application of an amplicon-based next-generation sequencing (NGS) assay to detect and identify trypanosomatid species in mammalian reservoirs, human patients, and sand fly vectors throughout regions of Leishmania endemicity. Sixty-five samples from different departments of Colombia, including two samples from Venezuela, were analyzed: 49 samples from cutaneous leishmaniasis (CL) patients, 8 from sand flies, 2 from domestic reservoirs (Canis familiaris), and 6 from wild reservoirs (Phyllostomus hastatus). DNA from each sample served to identify the presence of trypanosomatids through conventional PCR using heat shock protein 70 (HSP70) gene as the target. PCR products underwent sequencing by Sanger sequencing and NGS, and trypanosomatid species were identified by using BLASTn against a reference database built from trypanosomatid-derived HSP70 sequences. The alpha and beta diversity indexes of amplicon sequence variants were calculated for each group. The results revealed the presence of mixed infections with more than two Leishmania species in 34% of CL samples analyzed. Trypanosoma cruzi was identified in samples from wild reservoirs, as well as in sand fly vectors. Coinfection events with three different Leishmania species were identified in domestic reservoirs. These findings depose the traditional paradigm of leishmaniasis as being a single-species-driven infection and redraw the choreography of host-pathogen interaction in the context of multiparasitism. Further research is needed to decipher how coinfections may influence disease progression. This knowledge is key to developing an integrated approach for diagnosis and treatment.Ciencias Exactas y Naturales15 papplication/pdfhttps://doi.org/10.1128/Spectrum.00652-21https://repositorio.udes.edu.co/handle/001/6171engDowning Tim, Dublin City UniversityUSA15219Patiño LH, Castillo-Castañeda AC, Muñoz M, Jaimes JE, Luna-Niño N, Hernández C, Ayala MS, Fuya P, Mendez C, HernándezPereira CE, Delgado L, Sandoval-Ramírez CM, Urbano P, Paniz-Mondolfi A, Ramírez JD. 2021. Development of an amplicon-based nextgeneration sequencing protocol to identify Leishmania species and other trypanosomatids in Leishmaniasis endemic areas. Microbiol Spectr 9:e00652-21. https://doi.org/10.1128/ Spectrum.00652-21.ScopusMicrobiology Spectrum© 2021 Patiño et al.info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://journals.asm.org/doi/pdf/10.1128/Spectrum.00652-21Amplicon-based NGSLeishmaniaNGSSpeciesDevelopment of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areasArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Todas las AudienciasPublicationORIGINALDevelopment of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas.pdfDevelopment of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas.pdfapplication/pdf259583https://repositorio.udes.edu.co/bitstreams/068e0ae5-a665-46d0-9af3-f377fe0acf60/download1a8b233f5ad5202895947d6d169c09e5MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/0ec0bd73-ee54-4a5f-bda6-76c19901a256/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTDevelopment of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas.pdf.txtDevelopment of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/d5393917-3110-4c72-a867-3760c3d155f6/download5dbe86c1111d64f45ba435df98fdc825MD53THUMBNAILDevelopment of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas.pdf.jpgDevelopment of an amplicon-based next-generation sequencing protocol to identify leishmania species and other trypanosomatids in leishmaniasis endemic areas.pdf.jpgGenerated Thumbnailimage/jpeg11712https://repositorio.udes.edu.co/bitstreams/f6c6f502-8f20-4ac6-b79d-775e5c792f03/download3b00e56533db76f8a1e74776d5c864e4MD54001/6171oai:repositorio.udes.edu.co:001/61712023-10-11 10:11:52.478https://creativecommons.org/licenses/by-nc/4.0/© 2021 Patiño et al.https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |