Actividad Antimicrobiana de Aceites Esenciales Derivados de Lippia alba Sobre los Odontopatógenos Candida albicans y Aggregatibacter actinomycetemcomitans

Digital

Autores:
Caicedo-Jiménez, Karen Alexandra
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2021
Institución:
Universidad de Santander
Repositorio:
Repositorio Universidad de Santander
Idioma:
spa
OAI Identifier:
oai:repositorio.udes.edu.co:001/5930
Acceso en línea:
https://repositorio.udes.edu.co/handle/001/5930
Palabra clave:
Antimicrobiano
Fungicida
Bactericida
Lippia alba
Antimicrobial
Fungicidal
Bactericidal
Rights
openAccess
License
Derechos Reservados - Universidad de Santander, 2021
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dc.title.spa.fl_str_mv Actividad Antimicrobiana de Aceites Esenciales Derivados de Lippia alba Sobre los Odontopatógenos Candida albicans y Aggregatibacter actinomycetemcomitans
title Actividad Antimicrobiana de Aceites Esenciales Derivados de Lippia alba Sobre los Odontopatógenos Candida albicans y Aggregatibacter actinomycetemcomitans
spellingShingle Actividad Antimicrobiana de Aceites Esenciales Derivados de Lippia alba Sobre los Odontopatógenos Candida albicans y Aggregatibacter actinomycetemcomitans
Antimicrobiano
Fungicida
Bactericida
Lippia alba
Antimicrobial
Fungicidal
Bactericidal
title_short Actividad Antimicrobiana de Aceites Esenciales Derivados de Lippia alba Sobre los Odontopatógenos Candida albicans y Aggregatibacter actinomycetemcomitans
title_full Actividad Antimicrobiana de Aceites Esenciales Derivados de Lippia alba Sobre los Odontopatógenos Candida albicans y Aggregatibacter actinomycetemcomitans
title_fullStr Actividad Antimicrobiana de Aceites Esenciales Derivados de Lippia alba Sobre los Odontopatógenos Candida albicans y Aggregatibacter actinomycetemcomitans
title_full_unstemmed Actividad Antimicrobiana de Aceites Esenciales Derivados de Lippia alba Sobre los Odontopatógenos Candida albicans y Aggregatibacter actinomycetemcomitans
title_sort Actividad Antimicrobiana de Aceites Esenciales Derivados de Lippia alba Sobre los Odontopatógenos Candida albicans y Aggregatibacter actinomycetemcomitans
dc.creator.fl_str_mv Caicedo-Jiménez, Karen Alexandra
dc.contributor.advisor.none.fl_str_mv Guerra Sierra, Beatriz Elena
Quintero, Wendy Lorena
dc.contributor.author.none.fl_str_mv Caicedo-Jiménez, Karen Alexandra
dc.subject.proposal.spa.fl_str_mv Antimicrobiano
Fungicida
Bactericida
Lippia alba
topic Antimicrobiano
Fungicida
Bactericida
Lippia alba
Antimicrobial
Fungicidal
Bactericidal
dc.subject.proposal.eng.fl_str_mv Antimicrobial
Fungicidal
Bactericidal
description Digital
publishDate 2021
dc.date.issued.none.fl_str_mv 2021-11-26
dc.date.accessioned.none.fl_str_mv 2022-01-19T20:23:44Z
dc.date.available.none.fl_str_mv 2022-01-19T20:23:44Z
dc.type.spa.fl_str_mv Trabajo de grado - Pregrado
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dc.identifier.uri.none.fl_str_mv https://repositorio.udes.edu.co/handle/001/5930
identifier_str_mv T 33.21 C141a
url https://repositorio.udes.edu.co/handle/001/5930
dc.language.iso.spa.fl_str_mv spa
language spa
dc.rights.spa.fl_str_mv Derechos Reservados - Universidad de Santander, 2021
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dc.rights.creativecommons.spa.fl_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
dc.rights.uri.spa.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
rights_invalid_str_mv Derechos Reservados - Universidad de Santander, 2021
Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
https://creativecommons.org/licenses/by-nc-nd/4.0/
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eu_rights_str_mv openAccess
dc.format.extent.spa.fl_str_mv 110 p
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dc.publisher.spa.fl_str_mv Universidad de Santander
dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias Exactas, Naturales y Agropecuarias
dc.publisher.place.spa.fl_str_mv Bucaramanga, Colombia
dc.publisher.program.spa.fl_str_mv Microbiología Industrial
institution Universidad de Santander
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spelling Guerra Sierra, Beatriz Elena5b0af8c8-1ba6-4f54-9739-d7afb9538927-1Quintero, Wendy Lorenaed84c51a-8cab-44a8-8a34-a46a74d538e8-1Caicedo-Jiménez, Karen Alexandraf2327a1b-6f5f-4621-8014-76e6709e3006-12022-01-19T20:23:44Z2022-01-19T20:23:44Z2021-11-26DigitalEl presente estudio tuvo como objetivo evaluar la actividad antimicrobiana de aceites esenciales derivados de Lippia alba sobre Candida albicans y Aggregatibacter actinomycetemcomitans causantes de infecciones orales. Inicialmente, se evaluó la cinética de crecimiento de los microorganismos durante 48 h mediante espectrofotometría (600 nm), posteriormente se determinó la actividad antimicrobiana in vitro de aceites esenciales (AEs) derivados de L. alba y de sus terpenos bioactivos (óxido de cariofileno, citral y carvona) mediante microdilución en caldo (de forma individual y en combinación) y su actividad fungicida y bactericida. Los resultados fueron expresados como la concentración inhibitoria mínima (CIM) y el análisis estadístico se realizó mediante el uso del software (GraphPad Prism 5.0). Se encontró que los AEs del quimiotipo citral (F2 y F4) exhibieron la mejor actividad antimicrobiana (C. albicans: CIM 500μg/mL, concentración fungicida mínima (CFM) 1000 μg/mL; A. actinomycemtemcomitans: CIM 250 μg/mL y concentación bactericida mínima (CBM) 500 μg/mL; que las alcanzadas con los aceites del quimiotipo carvona y las referencias comerciales. Finalmente, los terpenos del quimiotipo citral (F2 y F4) evidenciaron la mejor interacción sinérgica in vitro contra la bacteria y (citral+OCN) para la levadura (CIF=0.41±0.11; μg/mL). Los resultados obtenidos representan una alternativa promisoria para el control de los microorganismos que causan la periodontitis.The present study aimed to evaluate the antimicrobial activity of essential oils derived from Lippia alba on Candida albicans and Aggregatibacter actinomycetemcomitans causing oral infections. Initially, the growth kinetics of microorganisms were evaluated during 48 h by spectrophotometry (600 nm), in vitro antimicrobial activity of essential oils (EO) derived from L. alba and its bioactive terpenes (caryophyllene, citral and carvone oxide) were subsequently determined by microdilution in broth (individually and in combination) and their fungicidal and bactericidal activity. The results were expressed as the minimum inhibitory concentration (MIC) and the statistical analysis was performed using GraphPad Prism 5.0 software. It was found that the EO of the citral chemotype (F2 and F4) exhibited the best antimicrobial activity (C. albicans: MIC 500μg/mL, minimum fungicidal concentration (MFC) 1000 μg/mL; A. actinomycemtemcomitans: CIM 250 μg/mL and minimum bactericidal concentration (MBC) 500 μg/mL; than achieved with the oils of the chemotype carvona and commercial references. Finally, the terpenes of the citral chemotype (F2 and F4) showed the best in vitro synergistic interaction against bacteria and (citral+OCN) for yeast. The results obtained represent a promising alternative for the control of microorganisms that cause periodontitisPregradoMicrobiólogo Industrial1° ed.Introducción................................................................................................................. 17 Planteamiento del Problema .............................................................................. 20 Justificación del Problema................................................................................. 23 Pregunta de Investigación....................................................................................... 26 Hipótesis............................................................................................................. 27 4.1 Hipótesis Alternativa..................................................................................... 27 4.2 Hipótesis Nula................................................................................................... 27 Marco Teórico.................................................................................................... 28 5.1 El Periodonto.................................................................................................... 28 5.1.1 La Encía............................................................................................................ 28 5.1.2 El Ligamento Periodontal................................................................................. 29 5.1.3 El Cemento Radicular........................................................................................ 29 5.1.4 El hueso Alveolar........................................................................................... 29 5.2 Infecciones Orales......................................................................................... 29 5.2.1 Enfermedad Periodontal............................................................................ 30 5.2.2 Periodontitis...................................................................................................... 30 5.2.2.1 Clasificación....................................................................................................... 5.3 Microbiota Oral.................................................................................................... 31 5.3.1 Microorganismos en las Infecciones Odontogénicas..................................... 32 5.3.2 Candida albicans............................................................... 32 5.3.3 Morfología...................................................................................................... 32 5.3.4 Patogénesis y Factores de Virulencia........................................................... 33 5.3.5 Aggregatibacter actinomycetemcomitans..................................................... 34 5.3.6 Morfología.................................................................................................... 35 5.3.7 Factores de Virulencia.................................................................................. 35 5.4 Tratamientos Clásicos....................................................................................... 37 5.4.1 Clorhexidina...................................................................................................... 37 5.4.2 Mecanismo de Acción................................................................................... 37 5.4.3 Tetraciclinas.................................................................................................. 38 5.5 Tratamientos Alternativos............................................................................... 39 5.5.1 Lippia alba...................................................................................................... 39 5.5.2 Actividad Biológica de Lippia alba................................................................. 42 5.5.3 Actividad Antifúngica de Lippia alba.......................................................... 42 5.5.4 Actividad Antibacteriana de Lippia alba...................................................... 43 5.6 Aceites Esenciales........................................................................................... 43 5.6.1 Actividad Antimicrobiana de los Aceites Esenciales.................................... 45 5.6.2 Terpenos de L. alba........................................................................................ 45 5.6.3 Citral............................................................................................................... 46 5.6.4 Óxido de Cariofileno..................................................................................... 46 5.6.5 Carvona.......................................................................................................... 47 Antecedentes.......................................................................................... 48 Objetivos............................................................................................................. 52 7.1 Objetivo General ............................................................................................ 52 7.2 Objetivos Específicos...................................................................................... 52 8 Metodología..................................................................................................... 53 8.1 Diseño del Estudio ......................................................................................... 53 8.2 Materiales........................................................................................................ 53 8.2.1 Candida albicans (ATCC 18804)...................................................................... 54 8.2.2 Aggregatibacter actinomycetemcomitans (ATCC 29522) ........................ 54 8.2.3 Aceites Esenciales Derivados de L. alba ..................................................... 54 8.3 Recolección de la Información ...................................................................... 54 8.3.1 Curva de Crecimiento ................................................................................ 54 8.3.2 Concentración Inhibitoria Mínima (CIM) ..................................................... 55 8.3.3 Determinación de la Concentración Fungicida Mínima (CFM) y Concentración Mínima Bactericida (CBM) ................................................................................... 57 8.4 Interacciones Farmacológicas Sobre los Odontopatógenos........................... 57 8.5 Análisis de Datos............................................................................................ 60 Resultados....................................................................................................... 61 9.1 Interacción Farmacológica de los Terpenos Sobre C. albicans y A actinomycetemcomitans....................................................................................... 67 Discusión.......................................................................................................... 72 Conclusiones...................................................................................................... 81 Recomendaciones.............................................................................................. 82 Referencias Bibliográficas....................................................................................... 83110 papplication/pdfT 33.21 C141ahttps://repositorio.udes.edu.co/handle/001/5930spaUniversidad de SantanderFacultad de Ciencias Exactas, Naturales y AgropecuariasBucaramanga, ColombiaMicrobiología IndustrialDerechos Reservados - Universidad de Santander, 2021info:eu-repo/semantics/openAccessAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)https://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_abf2AntimicrobianoFungicidaBactericidaLippia albaAntimicrobialFungicidalBactericidalActividad Antimicrobiana de Aceites Esenciales Derivados de Lippia alba Sobre los Odontopatógenos Candida albicans y Aggregatibacter actinomycetemcomitansTrabajo de grado - Pregradohttp://purl.org/coar/resource_type/c_7a1fTextinfo:eu-repo/semantics/bachelorThesishttps://purl.org/redcol/resource_type/TPhttp://purl.org/coar/version/c_71e4c1898caa6e32Todas las AudienciasÅberg, C. H., Kelk, P., & Johansson, A. (2015). Aggregatibacter actinomycetemcomitans: virulence of its leukotoxin and association with aggressive periodontitis. Virulence, 6(3), 188-195.Aggarwal, K., Khanuja, S., Ahmad, A., Santha, T., Gupta, V., & Kumar, S. (2002). Antimicrobial activity profiles of the two enantiomers of limonene and carvone isolated from the oils of Mentha spicata and Anethum sowa. Flavour and Fragrance Journal, 17(1), 59-63.Aguiar, J., Costa, M., Nascimento, S., & Sena, K. (2008). Antimicrobial activity of Lippia alba (Mill.) NE Brown (Verbenaceae). Revista Brasileira de Farmacognosia, 18(3), 436-440.Aguilera, L. (2009). Estudio de Pga26, una proteína implicada en la arquitectura de la pared celular de Candida albicans.Akkaoui, S., Johansson, A., Yagoubi, M., Haubek, D., Rida, S., Claesson, R., & Ennibi, O. (2020). 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Fluconazole MIC and the fluconazole dose/MIC ratio correlate with therapeutic response among patients with candidemia. Antimicrob Agents Chemother. 49:3171–7.CLSI. (2017). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeast M27 4th EditionCLSI. (2018). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Anaerobic Bacteria M11 9th EditionCoelho, A., Laranjo, M., Gonçalves, A., Paula, A., Paulo, S., Abrantes, A., ... & Botelho, M. (2020). Cytotoxic effects of a chlorhexidine mouthwash and of an enzymatic mouthwash on human gingival fibroblasts. Odontology, 108(2), 260-270.Costa, D., Vermelho, A., Almeida, C., Dias, E., Cedrola, S., ArrigoniBlank, M., & Alviano, D. (2014). Inhibitory effect of linalool-rich essential oil from Lippia alba on the peptidase and keratinase activities of dermatophytes. 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