Protein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites
Digital
- Autores:
-
Díaz-Hernández, Mitzi
Javier-Reyna, Rosario
Sotto-Ortega, Izaid José
García-Rivera, Guillermina
Montaño, Sarita
Betanzo, Abigail
Zanatta, Dxinegueela
Orozco, Esther
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2021
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/6197
- Acceso en línea:
- https://doi.org/10.3390/ijms22115709
https://repositorio.udes.edu.co/handle/001/6197
- Palabra clave:
- SUMOylation
Phagocytosis
E. histolytica
ESCRT machinery
EhADH adhesin
- Rights
- openAccess
- License
- © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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Protein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites |
| title |
Protein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites |
| spellingShingle |
Protein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites SUMOylation Phagocytosis E. histolytica ESCRT machinery EhADH adhesin |
| title_short |
Protein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites |
| title_full |
Protein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites |
| title_fullStr |
Protein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites |
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Protein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites |
| title_sort |
Protein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites |
| dc.creator.fl_str_mv |
Díaz-Hernández, Mitzi Javier-Reyna, Rosario Sotto-Ortega, Izaid José García-Rivera, Guillermina Montaño, Sarita Betanzo, Abigail Zanatta, Dxinegueela Orozco, Esther |
| dc.contributor.author.none.fl_str_mv |
Díaz-Hernández, Mitzi Javier-Reyna, Rosario Sotto-Ortega, Izaid José García-Rivera, Guillermina Montaño, Sarita Betanzo, Abigail Zanatta, Dxinegueela Orozco, Esther |
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Ciencia UDES |
| dc.subject.proposal.eng.fl_str_mv |
SUMOylation Phagocytosis E. histolytica ESCRT machinery EhADH adhesin |
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SUMOylation Phagocytosis E. histolytica ESCRT machinery EhADH adhesin |
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Digital |
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2021 |
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2021-05-27 |
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2022-03-01T15:52:34Z |
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eng |
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Díaz-Hernández, M.; Javier-Reyna, R.; Sotto-Ortega, I.; García-Rivera, G.; Montaño, S.; Betanzos, A.; Zanatta, D.; Orozco, E. Protein Sumoylation Is Crucial for Phagocytosis in Entamoeba histolytica Trophozoites. Int. J. Mol. Sci. 2021, 22, 5709. https://doi.org/10.3390/ ijms22115709 |
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Scopus |
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International Journal of Molecular Sciences |
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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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24 p |
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Díaz-Hernández, Mitzi6669a085-1a0d-4083-9960-14f27f5570e1-1Javier-Reyna, Rosarioc3c19bbe-34a6-43d0-8d87-7ddb6e142e88-1Sotto-Ortega, Izaid Joséf3a638d7-635f-49c5-b4fe-f28651f9949c-1García-Rivera, Guillerminae03a90cf-665f-405c-b69d-8412f37dc02f-1Montaño, Saritac42310f6-eefc-4eb4-a0a7-798127d5a261-1Betanzo, Abigail41f5c8f4-6487-41e0-a2d2-e16d6bba288f-1Zanatta, Dxinegueelaf5bf2b1c-9ea9-42eb-ac51-bc59541bcdb6-1Orozco, Esther265fc2d6-247b-4034-9896-927370abb5e3-1Ciencia UDES2022-03-01T15:52:34Z2022-03-01T15:52:34Z2021-05-27DigitalPosttranslational modifications provide Entamoeba histolytica proteins the timing and signaling to intervene during different processes, such as phagocytosis. However, SUMOylation has not been studied in E. histolytica yet. Here, we characterized the E. histolytica SUMO gene, its product (EhSUMO), and the relevance of SUMOylation in phagocytosis. Our results indicated that EhSUMO has an extended N-terminus that differentiates SUMO from ubiquitin. It also presents the GG residues at the C-terminus and the ΨKXE/D binding motif, both involved in target protein contact. Additionally, the E. histolytica genome possesses the enzymes belonging to the SUMOylation-deSUMOylation machinery. Confocal microscopy assays disclosed a remarkable EhSUMO membrane activity with convoluted and changing structures in trophozoites during erythrophagocytosis. SUMOylated proteins appeared in pseudopodia, phagocytic channels, and around the adhered and ingested erythrocytes. Docking analysis predicted interaction of EhSUMO with EhADH (an ALIX family protein), and immunoprecipitation and immunofluorescence assays revealed that the association increased during phagocytosis; whereas the EhVps32 (a protein of the ESCRT-III complex)-EhSUMO interaction appeared stronger since basal conditions. In EhSUMO knocked-down trophozoites, the bizarre membranous structures disappeared, and EhSUMO interaction with EhADH and EhVps32 diminished. Our results evidenced the presence of a SUMO gene in E. histolytica and the SUMOylation relevance during phagocytosis. This is supported by bioinformatics screening of many other proteins of E. histolytica involved in phagocytosis, which present putative SUMOylation sites and the ΨKXE/D binding motif.Ciencias Médicas y de la Salud24 papplication/pdfhttps://doi.org/10.3390/ijms22115709https://repositorio.udes.edu.co/handle/001/6197engSuiza2411122Díaz-Hernández, M.; Javier-Reyna, R.; Sotto-Ortega, I.; García-Rivera, G.; Montaño, S.; Betanzos, A.; Zanatta, D.; Orozco, E. Protein Sumoylation Is Crucial for Phagocytosis in Entamoeba histolytica Trophozoites. Int. J. Mol. Sci. 2021, 22, 5709. https://doi.org/10.3390/ ijms22115709ScopusInternational Journal of Molecular Sciences© 2021 by the authors. Licensee MDPI, Basel, Switzerland.info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://www.mdpi.com/1422-0067/22/11/5709SUMOylationPhagocytosisE. histolyticaESCRT machineryEhADH adhesinProtein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoitesArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Todas las AudienciasPublicationORIGINALProtein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites.pdfProtein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites.pdfapplication/pdf232208https://repositorio.udes.edu.co/bitstreams/75a3d0f5-ad29-49c7-a749-c8cbf3770276/downloade3443ed299311af184118216f126fc0cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/439db64a-0bdb-484d-bfb1-4cfc5a3165da/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTProtein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites.pdf.txtProtein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/b2b271a3-0e49-4004-93c1-8742d08596e4/download5dbe86c1111d64f45ba435df98fdc825MD53THUMBNAILProtein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites.pdf.jpgProtein sumoylation is crucial for phagocytosis in entamoeba histolytica trophozoites.pdf.jpgGenerated Thumbnailimage/jpeg12689https://repositorio.udes.edu.co/bitstreams/f44d4a69-1bd2-46f7-94a5-8f191eeb301a/downloade55bc2b5aa709ea656eb35395e42ef05MD54001/6197oai:repositorio.udes.edu.co:001/61972023-10-10 17:10:57.296https://creativecommons.org/licenses/by-nc/4.0/© 2021 by the authors. 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