Polymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index

Digital

Autores:: León, Francisco Javier
Pérez-Forero, Viviana Lucía
Hernandez-Mateus, Luisa Fernanda
Herrera-Celis, Javier Orlando
Gil-Zapata, Adriana María
Pico-Romero, Adriana Lucía

Tipo de recurso:: Article of journal

Fecha de publicación:: 2019

Institución:: Universidad de Santander

Repositorio:: Repositorio Universidad de Santander

Idioma:: spa

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dc.title.spa.fl_str_mv	Polymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index
title	Polymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index
spellingShingle	Polymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index Polimorfismo genético ADRB3 Índice de masa corporal Obesidad Genetic Polymorphism Body Mass Index Obesity
title_short	Polymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index
title_full	Polymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index
title_fullStr	Polymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index
title_full_unstemmed	Polymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index
title_sort	Polymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index
dc.creator.fl_str_mv	León, Francisco Javier Pérez-Forero, Viviana Lucía Hernandez-Mateus, Luisa Fernanda Herrera-Celis, Javier Orlando Gil-Zapata, Adriana María Pico-Romero, Adriana Lucía
dc.contributor.author.none.fl_str_mv	León, Francisco Javier Pérez-Forero, Viviana Lucía Hernandez-Mateus, Luisa Fernanda Herrera-Celis, Javier Orlando Gil-Zapata, Adriana María Pico-Romero, Adriana Lucía
dc.contributor.researchgroup.spa.fl_str_mv	Cibas
dc.subject.proposal.spa.fl_str_mv	Polimorfismo genético ADRB3 Índice de masa corporal Obesidad
topic	Polimorfismo genético ADRB3 Índice de masa corporal Obesidad Genetic Polymorphism Body Mass Index Obesity
dc.subject.proposal.eng.fl_str_mv	Genetic Polymorphism Body Mass Index Obesity
description	Digital
publishDate	2019
dc.date.issued.none.fl_str_mv	2019-11-05
dc.date.accessioned.none.fl_str_mv	2022-04-27T16:49:18Z
dc.date.available.none.fl_str_mv	2022-04-27T16:49:18Z
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dc.identifier.doi.none.fl_str_mv	https://revista.nutricion.org/PDF/LEON.pdf
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dc.relation.citationvolume.spa.fl_str_mv	39
dc.relation.indexed.spa.fl_str_mv	Scopus
dc.relation.ispartofjournal.spa.fl_str_mv	Nutricion Clinica y Dietetica Hospitalaria
dc.rights.spa.fl_str_mv	© Copyright 2019. The Authors, Fundación Alimentación Saludable
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dc.format.extent.spa.fl_str_mv	6 p
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dc.publisher.place.spa.fl_str_mv	España
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spelling	León, Francisco Javierc7479a09-b113-4576-9945-1c830662fc25-1Pérez-Forero, Viviana Lucía9d0a3d6e-81b0-4252-ae29-b33254477627-1Hernandez-Mateus, Luisa Fernandaa95901f2-8904-4c6f-b894-74c452c4986b-1Herrera-Celis, Javier Orlando8839c6f0-8ab8-4bf9-96e6-b02bb86d68af-1Gil-Zapata, Adriana María37e86e76-c887-450d-8ef4-8c7ac8b35b04-1Pico-Romero, Adriana Lucía55a2cf21-5c76-4686-8f0b-a77f1977421b-1Cibas2022-04-27T16:49:18Z2022-04-27T16:49:18Z2019-11-05DigitalIntroducción: Para el 2007 en Colombia se estimó que el 56% de la población adulta era obesa, condición que se considera un problema de salud pública con implicaciones en el desarrollo de enfermedad coronaria. Objetivo: Determinar la distribución de las frecuencias alélicas y genotípicas y la relación del polimorfismo rs4998 del gen ADRB3 con el índice de masa corporal en una población de docentes y administrativos. Materiales y Métodos: La extracción de ADN se llevó a cabo mediante un método comercial, para la amplificación de ADN se emplearon cebadores específicos no marcado con fluorocromos, los fragmentos obtenidos fueron purificados mediante las enzimas SAP y EXOSAP, finalmente se realizó minisecuenciación en el ABI PRISM 310 y posterior lectura de electroferogramas. El análisis de las variables sociode - mográficas se realizó mediante métodos univariados y métodos bivariados empleando pruebas de significancia como: test Chi2 de Pearson y test exacto de Fisher. Modelos logísticos fueron empleados para evaluar asociación. Resultados: Las frecuencias genotípicas encontradas fueron 0.96 GG, 0.014 CG y 0,020 CC. Las frecuencias alélicas fueron 0,027 C y 0,973 G. La muestra se encontró en equilibrio de Hardy-Weinberg con Chi2 = 81.153(1) (p = 0.000) y se calculó la estructura poblacional mediante la prueba Fst = 0,743. Discusión: Encontrar la población en equilibrio de HardyWeinberg indica que los individuos se están mezclando al azar. El coeficiente de endogamia o estadístico Fst, indica que la población presenta una alta estructura o diferenciación genética y existe fijación del alelo mutado G; esto podría obtener falsas asociaciones con la patología de interés. Conclusión: Las frecuencias obtenidas en este estudio no se ajustan a los modelos génicos propuestos para estudios de asociación; por lo tanto, se recomienda aumentar el tamaño de la muestra para futuras investigaciones o plantear estudios de casos y controles.Introduction: At 2007 in Colombia it was estimated that a portion 56 % from the adult population was obese, a condition that is considered a public health problem with implications in the development of coronary heart disease. Objective: Determine the distribution of allelic and genotypic frequencies and the relation of the polymorphism rs4998 of the ADRB3 gene with the Body Mass Index in a population of teachers and administrators of an university institution in Colombia. Methods: DNA extraction was carried out by a commercial method, for specific DNA amplification, specific primers not labeled with fluorochromes were used, fragments obtained were purified by SAP and EXOSAP enzymes, and finally minisequencing was performed in the ABI PRISM 310 and later electropherogram reading. The analysis of the sociodemographic variables was performed using univariate methods and bivariate methods using significance tests such as: Pearson’s Chi2 test and Fisher’s exact test. Logistic models were used to evaluate association. Results: The genotype frequencies found were 0.96 GG, 0.014 CG and 0.020 CC. The allele frequencies were 0.027 C and 0.973 G. The sample was found in Hardy-Weinberg equilibrium with Chi2 = 81.153 (1) (p = 0.000) and the population structure was calculated by means of the Fst test = 0.743. Discussion: Finding the population in Hardy-Weinberg equilibrium, indicates that individuals were mixed randomly. The coefficient of inbreeding or statistical Fst, is very close to one, which indicates that the population has a high structure or genetic differentiation and there is fixation of the mutated allele G. This could obtain false associations with the pathology of interest. Conclusion: The frequencies obtained in this study do not fit the proposed gene models for association studies, therefore, it is recommended to increase the sample size for future researches or to propose case-control studies.Ciencias Naturales, Exactas y Agropecuarias6 papplication/pdfhttps://revista.nutricion.org/PDF/LEON.pdf0211-60571989-208Xhttps://repositorio.udes.edu.co/handle/001/6633spaEspaña4934439ScopusNutricion Clinica y Dietetica Hospitalaria© Copyright 2019. The Authors, Fundación Alimentación Saludableinfo:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://revista.nutricion.org/PDF/LEON.pdfPolimorfismo genéticoADRB3Índice de masa corporalObesidadGenetic PolymorphismBody Mass IndexObesityPolymorphism rs4998 of the ADRB3 gene and its relationship with the body mass indexArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Todas las AudienciasPublicationORIGINALPolymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index.pdfPolymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index.pdfapplication/pdf257131https://repositorio.udes.edu.co/bitstreams/73902e6a-fd06-4e6c-a43d-ca77d446dd2d/download338a0e7a8c4197e189806b91ad044005MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/de406da1-277b-46fa-97b3-bbc94df317b1/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTPolymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index.pdf.txtPolymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/c086c479-f84d-42a3-85ab-f213d1458e5d/download5dbe86c1111d64f45ba435df98fdc825MD53THUMBNAILPolymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index.pdf.jpgPolymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index.pdf.jpgGenerated Thumbnailimage/jpeg11659https://repositorio.udes.edu.co/bitstreams/82cc7647-4c16-44b6-8082-44ab79ff27c8/download7501d2c7b9e84c7fa9e554a2910ad0aaMD54001/6633oai:repositorio.udes.edu.co:001/66332023-10-11 09:09:52.991https://creativecommons.org/licenses/by-nc/4.0/© Copyright 2019. The Authors, Fundación Alimentación Saludablehttps://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo=

Polymorphism rs4998 of the ADRB3 gene and its relationship with the body mass index

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