Identification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa )
10 p.
- Autores:
-
Galvis Serrano, Nestor Fabián
Moreno, Laura Yolima
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/3174
- Acceso en línea:
- https://repositorio.udes.edu.co/handle/001/3174
- Palabra clave:
- Control biológico
Babosas
Molusquicidas
Bioensayos
Biological control
Slugs
Molluscicides
Bioassays
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Santander, 2018
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| dc.title.eng.fl_str_mv |
Identification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa ) |
| dc.title.alternative.spa.fl_str_mv |
Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa) |
| title |
Identification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa ) |
| spellingShingle |
Identification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa ) Control biológico Babosas Molusquicidas Bioensayos Biological control Slugs Molluscicides Bioassays |
| title_short |
Identification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa ) |
| title_full |
Identification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa ) |
| title_fullStr |
Identification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa ) |
| title_full_unstemmed |
Identification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa ) |
| title_sort |
Identification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa ) |
| dc.creator.fl_str_mv |
Galvis Serrano, Nestor Fabián Moreno, Laura Yolima |
| dc.contributor.author.spa.fl_str_mv |
Galvis Serrano, Nestor Fabián Moreno, Laura Yolima |
| dc.subject.proposal.spa.fl_str_mv |
Control biológico Babosas Molusquicidas Bioensayos Biological control |
| topic |
Control biológico Babosas Molusquicidas Bioensayos Biological control Slugs Molluscicides Bioassays |
| dc.subject.proposal.eng.fl_str_mv |
Slugs Molluscicides Bioassays |
| description |
10 p. |
| publishDate |
2018 |
| dc.date.issued.spa.fl_str_mv |
2018-05 |
| dc.date.accessioned.spa.fl_str_mv |
2019-06-20T20:59:25Z |
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2019-06-20T20:59:25Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
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info:eu-repo/semantics/article |
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http://purl.org/redcol/resource_type/ART |
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info:eu-repo/semantics/publishedVersion |
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publishedVersion |
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10.5154/r.rchsh.2017.07.025 1027-152X |
| dc.identifier.issn.spa.fl_str_mv |
2007-4034 |
| dc.identifier.uri.spa.fl_str_mv |
https://repositorio.udes.edu.co/handle/001/3174 |
| identifier_str_mv |
10.5154/r.rchsh.2017.07.025 1027-152X 2007-4034 |
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https://repositorio.udes.edu.co/handle/001/3174 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.spa.fl_str_mv |
Revista Chapingo, Serie Horticultura |
| dc.rights.spa.fl_str_mv |
Derechos Reservados - Universidad de Santander, 2018 |
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http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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https://creativecommons.org/licenses/by-nc/4.0/ |
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Derechos Reservados - Universidad de Santander, 2018 Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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http://www.scielo.org.mx/pdf/rcsh/v24n2/2007-4034-rcsh-24-02-97.pdf |
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Universidad de Santander |
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Galvis Serrano, Nestor Fabián214941d4-65ed-4726-86f0-cf28ab7b7511-1Moreno, Laura Yolima700ac6cf-0520-4d01-b567-1a21d8c4384a-12019-06-20T20:59:25Z2019-06-20T20:59:25Z2018-0510 p.Milax gagates constituye una de las principales plagas en el cultivo de lechuga causando considerables pérdidas económicas. El control con molusquicidas elimina total o parcialmente la plaga, pero genera un impacto negativo en el ambiente por su alta toxicidad. El manejo biológico también permite el control de la plaga, pero de manera específica e inocua para el ambiente. Por ello, el objetivo de este trabajo fue evaluar el efecto tóxico de Bacillus thuringiensis, con genes Cry1, contra M. gagates mediante la elaboración de un bioensayo. Se tomaron 75 muestras de suelo de varios municipios del Norte de Santander, Colombia, de donde se obtuvieron 58 colonias aisladas de B. thuringiensis con características similares. De estas colonias, cinco evidenciaron la presencia de genes Cry1, y fueron las empleadas en los bioensayos junto con el control B. thuringiensis var. Kurstaki. Los tratamientos presentaron letalidad ante M. gagates de entre 90 y 100 % a una concentración de 500 μg∙mL-1. La CL99 calculada sugiere el uso de 820 μg∙mL-1 del biopreparado para obtener 100 % de letalidad con cualquiera de los cinco aislados estudiados.Milax gagates is one of the main pests in the cultivation of lettuce, causing considerable economic losses. Control with molluscicides totally or partially eliminates the pest but generates a negative impact on the environment due to their high toxicity. Biological management also allows controlling the pest, but in a specific and safe way for the environment. Therefore, the objective of this work was to evaluate the toxic effect of Bacillus thuringiensis, with Cry1 genes, against M. gagates by means of a bioassay. Seventy-five soil samples were taken from several municipalities in Norte de Santander, Colombia, from where 58 isolated colonies of B. thuringiensis with similar characteristics were obtained. Of these colonies, five showed the presence of Cry1 genes, and were those used in the bioassays together with the control B. thuringiensis var. Kurstaki. The treatments caused 90-100 % lethality to M. gagates at a concentration of 500 μg∙mL-1. The calculated LD99 suggests the use of 820 μg∙mL-1 of the biopreparate to obtain 100 % lethality with any of the five isolates studied.application/pdf10.5154/r.rchsh.2017.07.0251027-152X2007-4034https://repositorio.udes.edu.co/handle/001/3174engRevista Chapingo, Serie HorticulturaDerechos Reservados - Universidad de Santander, 2018info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2http://www.scielo.org.mx/pdf/rcsh/v24n2/2007-4034-rcsh-24-02-97.pdfControl biológicoBabosasMolusquicidasBioensayosBiological controlSlugsMolluscicidesBioassaysIdentification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa )Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa)Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85PublicationTHUMBNAILIdentification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa ).pdf.jpgIdentification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa ).pdf.jpgGenerated Thumbnailimage/jpeg1661https://repositorio.udes.edu.co/bitstreams/a01745c1-4f9b-46fd-8607-8df620306b4a/download38a14b0ad64b07138a9d1bd2097e313bMD54ORIGINALIdentification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa ).pdfIdentification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa ).pdfapplication/pdf325802https://repositorio.udes.edu.co/bitstreams/3031f375-2834-43aa-9cbb-938d1f3d5cd4/download2d4c1e2abf10919fc20b53bd3eb68d6cMD55LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/c3716e58-ee53-4aac-9d51-4399120b0d07/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTIdentification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa ).pdf.txtIdentification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates , plague in lettuce ( Lactuca sativa ).pdf.txtExtracted texttext/plain49903https://repositorio.udes.edu.co/bitstreams/626ad69e-11c8-49be-82f6-192e2327633e/downloadec1818d58415988e67b186e8977a29fcMD53001/3174oai:repositorio.udes.edu.co:001/31742023-10-09 10:27:49.036https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Santander, 2018https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |