Protein Engineering of Bacillus thuringiensis δ-Endotoxins
11 p.
- Autores:
-
Florez, Alvaro M.
Osorio, Cristina
Alzate, Oscar
- Tipo de recurso:
- Part of book
- Fecha de publicación:
- 2012
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/3595
- Acceso en línea:
- https://repositorio.udes.edu.co/handle/001/3595
- Palabra clave:
- Protein engineering
Protein domains
Protein structure
Designing novel toxins
δ-endotoxins
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Santander, 2012
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Florez, Alvaro M.03b37924-311e-4ca6-bae7-fb60528ecb1c-1Osorio, Cristinad1053838-b1f0-4825-ab49-673f10e9521d-1Alzate, Oscar73209183-39f5-47d8-b148-c89b5056c6bb-12019-08-16T18:08:43Z2019-08-16T18:08:43Z2012-0311 p.Protein engineering of insecticidal Bt δ-endotoxins is a powerful tool for designing novel Cry toxins with altered properties, including changing the toxin’s specificity. By following some elementary rules governing the structure/function relationship, it has been possible to create new toxins with modified properties including increased toxicity and binding affinity, enhanced ion-transport activity, and changes in insect specificity. These methods have also produced valuable information and have led to an improved understanding of the mode of action of these important biopesticides. The results discussed in this chapter derive from rational molecular design where protein structure is modified by incorporating single or multiple amino acid substitutions aimed at modifying specific protein functions. In this review, we analyze several protein modifications that have been successfully used for creating stable, functional proteins with minimal structural alterations. The understanding and proper use of protein engineering approaches may help in implementing appropriate pest management strategies by improving the efficacy of these toxins against insect pests.application/pdf10.1007/978-94-007-3021-2_5978-94-007-3021-2https://repositorio.udes.edu.co/handle/001/3595engBacillus thuringiensis BiotechnologyBacillus thuringiensis Biotechnology;Derechos Reservados - Universidad de Santander, 2012info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2https://link.springer.com/chapter/10.1007%2F978-94-007-3021-2_5Protein engineeringProtein domainsProtein structureDesigning novel toxinsδ-endotoxinsProtein Engineering of Bacillus thuringiensis δ-EndotoxinsCapítulo - Parte de Librohttp://purl.org/coar/resource_type/c_3248Textinfo:eu-repo/semantics/bookParthttps://purl.org/redcol/resource_type/CAP_LIBinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85PublicationORIGINALProtein Engineering of Bacillus thuringiensis δ-Endotoxins.pdfProtein Engineering of Bacillus thuringiensis δ-Endotoxins.pdfapplication/pdf247927https://repositorio.udes.edu.co/bitstreams/1b58d0bd-e6c5-40fc-998f-921714859372/download5dc432842285307425053a319db91597MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/77e14065-6573-40d6-bdd7-f4b83165f65b/download38d94cf55aa1bf2dac1a736ac45c881cMD52THUMBNAILProtein Engineering of Bacillus thuringiensis δ-Endotoxins.PNG.jpgProtein Engineering of Bacillus thuringiensis δ-Endotoxins.PNG.jpgGenerated Thumbnailimage/jpeg1434https://repositorio.udes.edu.co/bitstreams/b6b062e1-5d01-4f19-817e-fe892c27a099/download4ec065fd838ba1ebfd720cda4144f427MD53Protein Engineering of Bacillus thuringiensis δ-Endotoxins.pdf.jpgProtein Engineering of Bacillus thuringiensis δ-Endotoxins.pdf.jpgGenerated Thumbnailimage/jpeg9769https://repositorio.udes.edu.co/bitstreams/2d7a77d5-c8bd-4a09-8b75-fb6b0ec965c3/download7cbf4d19541788d9503df7f6884cc49aMD56TEXTProtein Engineering of Bacillus thuringiensis δ-Endotoxins.pdf.txtProtein Engineering of Bacillus thuringiensis δ-Endotoxins.pdf.txtExtracted texttext/plain10https://repositorio.udes.edu.co/bitstreams/f9e0d449-6632-4173-815b-12ebb1bf5ebc/download030206d61a2c6007eb0788e87d1c5e07MD55001/3595oai:repositorio.udes.edu.co:001/35952023-10-09 10:06:55.58https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Santander, 2012https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |
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Protein Engineering of Bacillus thuringiensis δ-Endotoxins |
| title |
Protein Engineering of Bacillus thuringiensis δ-Endotoxins |
| spellingShingle |
Protein Engineering of Bacillus thuringiensis δ-Endotoxins Protein engineering Protein domains Protein structure Designing novel toxins δ-endotoxins |
| title_short |
Protein Engineering of Bacillus thuringiensis δ-Endotoxins |
| title_full |
Protein Engineering of Bacillus thuringiensis δ-Endotoxins |
| title_fullStr |
Protein Engineering of Bacillus thuringiensis δ-Endotoxins |
| title_full_unstemmed |
Protein Engineering of Bacillus thuringiensis δ-Endotoxins |
| title_sort |
Protein Engineering of Bacillus thuringiensis δ-Endotoxins |
| dc.creator.fl_str_mv |
Florez, Alvaro M. Osorio, Cristina Alzate, Oscar |
| dc.contributor.author.spa.fl_str_mv |
Florez, Alvaro M. Osorio, Cristina Alzate, Oscar |
| dc.subject.proposal.eng.fl_str_mv |
Protein engineering Protein domains Protein structure Designing novel toxins δ-endotoxins |
| topic |
Protein engineering Protein domains Protein structure Designing novel toxins δ-endotoxins |
| description |
11 p. |
| publishDate |
2012 |
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2012-03 |
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2019-08-16T18:08:43Z |
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2019-08-16T18:08:43Z |
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eng |
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Bacillus thuringiensis Biotechnology |
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Bacillus thuringiensis Biotechnology; |
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Derechos Reservados - Universidad de Santander, 2012 |
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