Ib-M6 Antimicrobial Peptide
1 p.
- Autores:
-
Flórez Castillo, Johanna-Marcela
Rondón Villarreal, Paola
Ropero Vega, José Luis
Mendoza Espinel, Stephany Y.
Moreno Amézquita, July A.
Méndez Jaimes, Karen D.
Farfán García, Ana Elvira
Gómez Rangel, Sergio-Yebrail
Gómez-Duarte, Oscar G.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2020
- Institución:
- Universidad de Santander
- Repositorio:
- Repositorio Universidad de Santander
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.udes.edu.co:001/4696
- Acceso en línea:
- https://repositorio.udes.edu.co/handle/001/4696
- Palabra clave:
- Péptidos antimicrobianos
Acoplamiento molecular
Escherichia coli
Antimicrobial peptides
Molecular docking
- Rights
- restrictedAccess
- License
- Derechos Reservados - The authors. Licensee MDPI, Basel, Switzerland, 2020
| id |
RUDES2_047dde5848ee3135766e7ec8f78ba672 |
|---|---|
| oai_identifier_str |
oai:repositorio.udes.edu.co:001/4696 |
| network_acronym_str |
RUDES2 |
| network_name_str |
Repositorio Universidad de Santander |
| repository_id_str |
|
| dc.title.eng.fl_str_mv |
Ib-M6 Antimicrobial Peptide |
| dc.title.alternative.eng.fl_str_mv |
Antibacterial Activity against Clinical Isolates of Escherichia coli and Molecular Docking |
| title |
Ib-M6 Antimicrobial Peptide |
| spellingShingle |
Ib-M6 Antimicrobial Peptide Péptidos antimicrobianos Acoplamiento molecular Escherichia coli Antimicrobial peptides Molecular docking |
| title_short |
Ib-M6 Antimicrobial Peptide |
| title_full |
Ib-M6 Antimicrobial Peptide |
| title_fullStr |
Ib-M6 Antimicrobial Peptide |
| title_full_unstemmed |
Ib-M6 Antimicrobial Peptide |
| title_sort |
Ib-M6 Antimicrobial Peptide |
| dc.creator.fl_str_mv |
Flórez Castillo, Johanna-Marcela Rondón Villarreal, Paola Ropero Vega, José Luis Mendoza Espinel, Stephany Y. Moreno Amézquita, July A. Méndez Jaimes, Karen D. Farfán García, Ana Elvira Gómez Rangel, Sergio-Yebrail Gómez-Duarte, Oscar G. |
| dc.contributor.author.spa.fl_str_mv |
Flórez Castillo, Johanna-Marcela Rondón Villarreal, Paola Ropero Vega, José Luis Mendoza Espinel, Stephany Y. Moreno Amézquita, July A. Méndez Jaimes, Karen D. Farfán García, Ana Elvira Gómez Rangel, Sergio-Yebrail Gómez-Duarte, Oscar G. |
| dc.contributor.corporatename.spa.fl_str_mv |
Universidad de Santander |
| dc.subject.proposal.spa.fl_str_mv |
Péptidos antimicrobianos Acoplamiento molecular |
| topic |
Péptidos antimicrobianos Acoplamiento molecular Escherichia coli Antimicrobial peptides Molecular docking |
| dc.subject.proposal.eng.fl_str_mv |
Escherichia coli Antimicrobial peptides Molecular docking |
| description |
1 p. |
| publishDate |
2020 |
| dc.date.accessioned.spa.fl_str_mv |
2020-08-20T17:21:11Z |
| dc.date.available.spa.fl_str_mv |
2020-08-20T17:21:11Z |
| dc.date.issued.spa.fl_str_mv |
2020-02-12 |
| dc.type.spa.fl_str_mv |
Artículo de revista |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
http://purl.org/coar/resource_type/c_6501 |
| status_str |
publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Flórez-Castillo, JM; Rondón-Villareal, P .; Ropero-Vega, JL; Mendoza-Espinel, SY; Moreno-Amézquita, JA; Méndez-Jaimes, KD; Farfán-García, AE; Gómez-Rangel, SY; Gómez-Duarte, OG Ib-M6 Péptido antimicrobiano: actividad antibacteriana frente a aislados clínicos de Escherichia coli y acoplamiento molecular. Antibióticos 2020 , 9 , 79. |
| dc.identifier.issn.spa.fl_str_mv |
2079-6382 |
| dc.identifier.uri.spa.fl_str_mv |
https://repositorio.udes.edu.co/handle/001/4696 |
| identifier_str_mv |
Flórez-Castillo, JM; Rondón-Villareal, P .; Ropero-Vega, JL; Mendoza-Espinel, SY; Moreno-Amézquita, JA; Méndez-Jaimes, KD; Farfán-García, AE; Gómez-Rangel, SY; Gómez-Duarte, OG Ib-M6 Péptido antimicrobiano: actividad antibacteriana frente a aislados clínicos de Escherichia coli y acoplamiento molecular. Antibióticos 2020 , 9 , 79. 2079-6382 |
| url |
https://repositorio.udes.edu.co/handle/001/4696 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.eng.fl_str_mv |
Antibiotics |
| dc.relation.ispartofseries.eng.fl_str_mv |
Antibiotics;2020, 9, 79 |
| dc.rights.spa.fl_str_mv |
Derechos Reservados - The authors. Licensee MDPI, Basel, Switzerland, 2020 |
| dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_16ec |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
| dc.rights.creativecommons.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0/ |
| rights_invalid_str_mv |
Derechos Reservados - The authors. Licensee MDPI, Basel, Switzerland, 2020 Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_16ec |
| eu_rights_str_mv |
restrictedAccess |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.publisher.eng.fl_str_mv |
Basilea : MDPI, 2020 |
| dc.source.eng.fl_str_mv |
https://www.mdpi.com/2079-6382/9/2/79 |
| institution |
Universidad de Santander |
| bitstream.url.fl_str_mv |
https://repositorio.udes.edu.co/bitstreams/0959f544-a06d-435c-ac78-60061499f6f4/download https://repositorio.udes.edu.co/bitstreams/11c2aab1-b1d5-49cf-8224-7833d3e508fd/download https://repositorio.udes.edu.co/bitstreams/8521e080-e570-4502-85df-1734539fe355/download https://repositorio.udes.edu.co/bitstreams/4c938ede-789d-4a11-9e44-2816e40472fd/download |
| bitstream.checksum.fl_str_mv |
c7848547e6791f12e6f5edf00903a8f8 38d94cf55aa1bf2dac1a736ac45c881c 5dbe86c1111d64f45ba435df98fdc825 1181ad621e2c4e290fa87d7f8d154583 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Universidad de Santander |
| repository.mail.fl_str_mv |
soporte@metabiblioteca.com |
| _version_ |
1818102005902606336 |
| spelling |
Flórez Castillo, Johanna-Marcelae5b9135c-adb9-4f3f-adf9-eae7cd9e6abf-1Rondón Villarreal, Paola8c5e399b-8f10-43d4-9834-7a43f426c16c-1Ropero Vega, José Luis6a26675d-491a-46a5-84d0-67b7a519e473-1Mendoza Espinel, Stephany Y.21317a74-3f72-41b2-b525-10cc63c1f349-1Moreno Amézquita, July A.81b7f9bd-dac0-4470-961d-b3c09718cfad-1Méndez Jaimes, Karen D.bad229d1-a756-44ef-b303-2e3275817e90-1Farfán García, Ana Elvira74472815-bd1a-4bf4-932b-0264dd209268-1Gómez Rangel, Sergio-Yebrail0347d3d7-298c-4b8a-a82b-e34275e1ea44-1Gómez-Duarte, Oscar G.a2dbbaaf-ccc7-43db-96d4-f7d083ad619e-1Universidad de Santander2020-08-20T17:21:11Z2020-08-20T17:21:11Z2020-02-121 p.El péptido Ib-M6 tiene actividad antibacteriana contra la cepa K-12 de Escherichia coli no patógena . La primera parte de este estudio determina la actividad antibacteriana de Ib-M6 contra catorce cepas patógenas de E. coli O157: H7. El ensayo de susceptibilidad mostró que Ib-M6 tenía valores de Concentración Inhibitoria Mínima (MIC) más bajos que la estreptomicina, usada como antibiótico de referencia. Además, para predecir la posible interacción entre Ib-M6 y los componentes de la membrana externa de E. coli, utilizamos simulaciones de acoplamiento molecular donde la proteína FhuA y su complejo con Lipopolisacárido (LPS-FhuA) se utilizaron como objetivos del péptido. Los complejos FhuA / Ib-M6 tenían valores de energía entre -39,5 y -40,5 unidades de energía Rosetta (REU) y solo un enlace de hidrógeno. En contraste, los complejos entre LPS-FhuA e Ib-M6 mostraron valores de energía entre -25,6 y -40,6 REU, y la presencia de cinco posibles enlaces de hidrógeno. Por tanto, la actividad antimicrobiana del péptido Ib-M6 mostrada en los ensayos experimentales podría deberse a su interacción con la membrana externa de E. coli .The Ib-M6 peptide has antibacterial activity against non-pathogenic Escherichia coli K-12 strain. The first part of this study determines the antibacterial activity of Ib-M6 against fourteen pathogenic strains of E. coli O157:H7. Susceptibility assay showed that Ib-M6 had values of Minimum Inhibitory Concentration (MIC) lower than streptomycin, used as a reference antibiotic. Moreover, to predict the possible interaction between Ib-M6 and outer membrane components of E. coli, we used molecular docking simulations where FhuA protein and its complex with Lipopolysaccharide (LPS–FhuA) were used as targets of the peptide. FhuA/Ib-M6 complexes had energy values between 39.5 and 40.5 Rosetta Energy Units (REU) and only one hydrogen bond. In contrast, complexes between LPS–FhuA and Ib-M6 displayed energy values between 25.6 and 40.6 REU, and the presence of five possible hydrogen bonds. Hence, the antimicrobial activity of Ib-M6 peptide shown in the experimental assays could be caused by its interaction with the outer membrane of E. coli.Ej. 1application/pdfFlórez-Castillo, JM; Rondón-Villareal, P .; Ropero-Vega, JL; Mendoza-Espinel, SY; Moreno-Amézquita, JA; Méndez-Jaimes, KD; Farfán-García, AE; Gómez-Rangel, SY; Gómez-Duarte, OG Ib-M6 Péptido antimicrobiano: actividad antibacteriana frente a aislados clínicos de Escherichia coli y acoplamiento molecular. Antibióticos 2020 , 9 , 79.2079-6382https://repositorio.udes.edu.co/handle/001/4696engBasilea : MDPI, 2020AntibioticsAntibiotics;2020, 9, 79Derechos Reservados - The authors. Licensee MDPI, Basel, Switzerland, 2020info:eu-repo/semantics/restrictedAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_16echttps://www.mdpi.com/2079-6382/9/2/79Péptidos antimicrobianosAcoplamiento molecularEscherichia coliAntimicrobial peptidesMolecular dockingIb-M6 Antimicrobial PeptideAntibacterial Activity against Clinical Isolates of Escherichia coli and Molecular DockingArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85PublicationORIGINALIb-M6 Antimicrobial Peptide. Antibacterial Activity against Clinical Isolates of Escherichia coli and Molecular Docking.pdfIb-M6 Antimicrobial Peptide. Antibacterial Activity against Clinical Isolates of Escherichia coli and Molecular Docking.pdfapplication/pdf298110https://repositorio.udes.edu.co/bitstreams/0959f544-a06d-435c-ac78-60061499f6f4/downloadc7848547e6791f12e6f5edf00903a8f8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-859https://repositorio.udes.edu.co/bitstreams/11c2aab1-b1d5-49cf-8224-7833d3e508fd/download38d94cf55aa1bf2dac1a736ac45c881cMD52TEXTIb-M6 Antimicrobial Peptide. Antibacterial Activity against Clinical Isolates of Escherichia coli and Molecular Docking.pdf.txtIb-M6 Antimicrobial Peptide. Antibacterial Activity against Clinical Isolates of Escherichia coli and Molecular Docking.pdf.txtExtracted texttext/plain5https://repositorio.udes.edu.co/bitstreams/8521e080-e570-4502-85df-1734539fe355/download5dbe86c1111d64f45ba435df98fdc825MD53THUMBNAILIb-M6 Antimicrobial Peptide. Antibacterial Activity against Clinical Isolates of Escherichia coli and Molecular Docking.pdf.jpgIb-M6 Antimicrobial Peptide. Antibacterial Activity against Clinical Isolates of Escherichia coli and Molecular Docking.pdf.jpgGenerated Thumbnailimage/jpeg12591https://repositorio.udes.edu.co/bitstreams/4c938ede-789d-4a11-9e44-2816e40472fd/download1181ad621e2c4e290fa87d7f8d154583MD54001/4696oai:repositorio.udes.edu.co:001/46962023-10-11 10:24:57.992https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - The authors. Licensee MDPI, Basel, Switzerland, 2020https://repositorio.udes.edu.coRepositorio Universidad de Santandersoporte@metabiblioteca.comTGljZW5jaWEgZGUgUHVibGljYWNpw7NuIFVERVMKRGlyZWN0cmljZXMgZGUgVVNPIHkgQUNDRVNPCgo= |