Erythropoietin reduces storage lesions and decreases apoptosis indices in blood bank red blood cells

6 páginas

Autores:: Penuela, Oscar Andrés
Palomino, Fernando
Gómez, Lina Andrea

Tipo de recurso:

Fecha de publicación:: 2016

Institución:: Universidad de la Sabana

Repositorio:: Repositorio Universidad de la Sabana

Idioma:: eng

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spelling	Penuela, Oscar AndrésPalomino, FernandoGómez, Lina Andrea9/18/2019 8:422019-09-18T13:42:03Z2016-011516-8484https://www.sciencedirect.com/science/article/pii/S1516848415001632?via%3Dihub#!https://reader.elsevier.com/reader/sd/pii/S1516848415001632?token=69F8AFCE769CB055E2092D8FF9250093A400821538EABFBE857F2041FBDE01C1597B5DA749E88DEE6B1CCBB9FC998B06http://hdl.handle.net/10818/3724110.1016/j.bjhh.2015.10.0036 páginasBackground Recent evidence shows a selective destruction of the youngest circulating red blood cells (neocytolysis) trigged by a drop in erythropoietin levels. Objective The aim of this study was to evaluate the effect of recombinant human erythropoietin beta on the red blood cell storage lesion and apoptosis indices under blood bank conditions. Methods Each one of ten red blood cell units preserved in additive solution 5 was divided in two volumes of 100 mL and assigned to one of two groups: erythropoietin (addition of 665 IU of recombinant human erythropoietin) and control (isotonic buffer solution was added). The pharmacokinetic parameters of erythropoietin were estimated and the following parameters were measured weekly, for six weeks: Immunoreactive erythropoietin, hemolysis, percentage of non-discocytes, adenosine triphosphate, glucose, lactate, lactate dehydrogenase, and annexin-V/esterase activity. The t-test or Wilcoxon's test was used for statistical analysis with significance being set for a p-value <0.05. Results Erythropoietin, when added to red blood cell units, has a half-life >6 weeks under blood bank conditions, with persistent supernatant concentrations of erythropoietin during the entire storage period. Adenosine triphosphate was higher in the Erythropoietin Group in Week 6 (4.19 ± 0.05 μmol/L vs. 3.53 ± 0.02 μmol/L; p-value = 0.009). The number of viable cells in the Erythropoietin Group was higher than in the Control Group (77% ± 3.8% vs. 71% ± 2.3%; p-value <0.05), while the number of apoptotic cells was lower (9.4% ± 0.3% vs. 22% ± 0.8%; p-value <0.05). Conclusions Under standard blood bank conditions, an important proportion of red blood cells satisfy the criteria of apoptosis. Recombinant human erythropoietin beta seems to improve storage lesion parameters and mitigate apoptosis.application/pdfengRevista Brasileira de Hematologia e HemoterapiaRevista Brasileira de Hematologia e Hemoterapia Volume 38, Issue 1, January–March 2016, Pages 15-2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_abf2Universidad de La SabanaIntellectum Repositorio Universidad de La SabanaBlood bankErythropoietinApoptosisStorage lesionErythropoietin reduces storage lesions and decreases apoptosis indices in blood bank red blood cellsarticlepublishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805https://intellectum.unisabana.edu.co/bitstream/10818/37241/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-8498https://intellectum.unisabana.edu.co/bitstream/10818/37241/3/license.txtf52a2cfd4df262e08e9b300d62c85cabMD5310818/37241oai:intellectum.unisabana.edu.co:10818/372412022-05-10 05:19:40.342Intellectum Universidad de la Sabanacontactointellectum@unisabana.edu.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
dc.title.es_CO.fl_str_mv	Erythropoietin reduces storage lesions and decreases apoptosis indices in blood bank red blood cells
title	Erythropoietin reduces storage lesions and decreases apoptosis indices in blood bank red blood cells
spellingShingle	Erythropoietin reduces storage lesions and decreases apoptosis indices in blood bank red blood cells Blood bank Erythropoietin Apoptosis Storage lesion
title_short	Erythropoietin reduces storage lesions and decreases apoptosis indices in blood bank red blood cells
title_full	Erythropoietin reduces storage lesions and decreases apoptosis indices in blood bank red blood cells
title_fullStr	Erythropoietin reduces storage lesions and decreases apoptosis indices in blood bank red blood cells
title_full_unstemmed	Erythropoietin reduces storage lesions and decreases apoptosis indices in blood bank red blood cells
title_sort	Erythropoietin reduces storage lesions and decreases apoptosis indices in blood bank red blood cells
dc.creator.fl_str_mv	Penuela, Oscar Andrés Palomino, Fernando Gómez, Lina Andrea
dc.contributor.author.none.fl_str_mv	Penuela, Oscar Andrés Palomino, Fernando Gómez, Lina Andrea
dc.subject.es_CO.fl_str_mv	Blood bank Erythropoietin Apoptosis Storage lesion
topic	Blood bank Erythropoietin Apoptosis Storage lesion
description	6 páginas
publishDate	2016
dc.date.issued.none.fl_str_mv	2016-01
dc.date.available.none.fl_str_mv	2019-09-18T13:42:03Z
dc.date.accessioned.none.fl_str_mv	9/18/2019 8:42
dc.type.en.fl_str_mv	article
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dc.identifier.issn.none.fl_str_mv	1516-8484
dc.identifier.other.none.fl_str_mv	https://www.sciencedirect.com/science/article/pii/S1516848415001632?via%3Dihub#! https://reader.elsevier.com/reader/sd/pii/S1516848415001632?token=69F8AFCE769CB055E2092D8FF9250093A400821538EABFBE857F2041FBDE01C1597B5DA749E88DEE6B1CCBB9FC998B06
dc.identifier.uri.none.fl_str_mv	http://hdl.handle.net/10818/37241
dc.identifier.doi.none.fl_str_mv	10.1016/j.bjhh.2015.10.003
identifier_str_mv	1516-8484 10.1016/j.bjhh.2015.10.003
url	https://www.sciencedirect.com/science/article/pii/S1516848415001632?via%3Dihub#! https://reader.elsevier.com/reader/sd/pii/S1516848415001632?token=69F8AFCE769CB055E2092D8FF9250093A400821538EABFBE857F2041FBDE01C1597B5DA749E88DEE6B1CCBB9FC998B06 http://hdl.handle.net/10818/37241
dc.language.iso.es_CO.fl_str_mv	eng
language	eng
dc.relation.ispartofseries.none.fl_str_mv	Revista Brasileira de Hematologia e Hemoterapia Volume 38, Issue 1, January–March 2016, Pages 15-2
dc.rights.*.fl_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.coar.fl_str_mv	http://purl.org/coar/access_right/c_abf2
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rights_invalid_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_abf2
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dc.publisher.es_CO.fl_str_mv	Revista Brasileira de Hematologia e Hemoterapia
dc.source.es_CO.fl_str_mv	Universidad de La Sabana Intellectum Repositorio Universidad de La Sabana
institution	Universidad de la Sabana
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Erythropoietin reduces storage lesions and decreases apoptosis indices in blood bank red blood cells

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