Regulation of human subcutaneous adipocyte differentiation by EID1

10 páginas

Autores:: Vargas, Diana
Shimokawa, Noriaki
Kaneko, Ryosuke
Rosales, Wendy
Parra, Adriana
Castellanos, Ángela
Koibuchi, Noriyuki
Lizcano Losada, Fernando

Tipo de recurso:

Fecha de publicación:: 2016

Institución:: Universidad de la Sabana

Repositorio:: Repositorio Universidad de la Sabana

Idioma:: eng

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spelling	Vargas, DianaShimokawa, NoriakiKaneko, RyosukeRosales, WendyParra, AdrianaCastellanos, ÁngelaKoibuchi, NoriyukiLizcano Losada, Fernando5/29/2018 15:062018-05-29T20:06:41Z2016-02Vargas, D., N, Shimokawa., R,,Kaneko., Parra, A., Castellanos, A, N., Lizcano, F.(2016) Regulation of human subcutaneous adipocyte differentiation by EID1, Journal of Molecular Endocrinology (56) 2 113-122http://jme.endocrinology-journals.org/content/56/2/113.full.pdf+html?sid=339bebb1-5536-46c8-918d-a4485ac0d0f0http://hdl.handle.net/10818/3306410.1530/JME-15-014810 páginasIncreasing thermogenesis in white adipose tissues can be used to treat individuals at high risk for obesity and cardiovascular disease. The objective of this study was to determine the function of EP300-interacting inhibitor of differentiation (EID1), an inhibitor of muscle differentiation, in the induction of beige adipocytes from adipose mesenchymal stem cells (ADMSCs). Subcutaneous adipose tissue was obtained from healthy women undergoing abdominoplasty. ADMSCs were isolated in vitro, grown, and transfected with EID1 or EID1 siRNA, and differentiation was induced after 48 h by administering rosiglitazone. The effects of EID1 expression under the control of the aP2 promoter (aP2-EID1) were also evaluated in mature adipocytes that were differentiated from ADMSCs. Transfection of EID1 into ADMSCs reduced triglyceride accumulation while increasing levels of thermogenic proteins, such as PGC1a, TFAM, and mitochondrial uncoupling protein 1 (UCP1), all of which are markers of energy expenditure and mitochondrial activity. Furthermore, increased expression of the beige phenotype markers CITED1 and CD137 was observed. Transfection of aP2-EID1 transfection induced the conversion of mature white adipocytes to beige adipocytes, as evidenced by increased expression of PGC1a, UCP1, TFAM, and CITED1. These results indicate that EID1 can modulate ADMSCs, inducing a brown/beige lineage. EID1 may also activate beiging in white adipocytes obtained from subcutaneous human adipose tissue.application/pdfengJournal of Molecular EndocrinologyJournal of Molecular Endocrinology (2016) 56, 113–122Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_abf2Universidad de La SabanaIntellectum Repositorio Universidad de La SabanaRetinoblastomaADMSCsPPARgEID1Beige fatRegulation of human subcutaneous adipocyte differentiation by EID1articlepublishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501LICENSElicense.txtlicense.txttext/plain; charset=utf-8498https://intellectum.unisabana.edu.co/bitstream/10818/33064/3/license.txtf52a2cfd4df262e08e9b300d62c85cabMD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81223https://intellectum.unisabana.edu.co/bitstream/10818/33064/2/license_rdf7c9ab7f006165862d8ce9ac5eac01552MD5210818/33064oai:intellectum.unisabana.edu.co:10818/330642022-05-10 05:16:35.971Intellectum Universidad de la Sabanacontactointellectum@unisabana.edu.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
dc.title.es_CO.fl_str_mv	Regulation of human subcutaneous adipocyte differentiation by EID1
title	Regulation of human subcutaneous adipocyte differentiation by EID1
spellingShingle	Regulation of human subcutaneous adipocyte differentiation by EID1 Retinoblastoma ADMSCs PPARg EID1 Beige fat
title_short	Regulation of human subcutaneous adipocyte differentiation by EID1
title_full	Regulation of human subcutaneous adipocyte differentiation by EID1
title_fullStr	Regulation of human subcutaneous adipocyte differentiation by EID1
title_full_unstemmed	Regulation of human subcutaneous adipocyte differentiation by EID1
title_sort	Regulation of human subcutaneous adipocyte differentiation by EID1
dc.creator.fl_str_mv	Vargas, Diana Shimokawa, Noriaki Kaneko, Ryosuke Rosales, Wendy Parra, Adriana Castellanos, Ángela Koibuchi, Noriyuki Lizcano Losada, Fernando
dc.contributor.author.none.fl_str_mv	Vargas, Diana Shimokawa, Noriaki Kaneko, Ryosuke Rosales, Wendy Parra, Adriana Castellanos, Ángela Koibuchi, Noriyuki Lizcano Losada, Fernando
dc.subject.none.fl_str_mv	Retinoblastoma ADMSCs PPARg EID1 Beige fat
topic	Retinoblastoma ADMSCs PPARg EID1 Beige fat
description	10 páginas
publishDate	2016
dc.date.issued.none.fl_str_mv	2016-02
dc.date.available.none.fl_str_mv	2018-05-29T20:06:41Z
dc.date.accessioned.none.fl_str_mv	5/29/2018 15:06
dc.type.en.fl_str_mv	article
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dc.identifier.citation.es_CO.fl_str_mv	Vargas, D., N, Shimokawa., R,,Kaneko., Parra, A., Castellanos, A, N., Lizcano, F.(2016) Regulation of human subcutaneous adipocyte differentiation by EID1, Journal of Molecular Endocrinology (56) 2 113-122
dc.identifier.other.none.fl_str_mv	http://jme.endocrinology-journals.org/content/56/2/113.full.pdf+html?sid=339bebb1-5536-46c8-918d-a4485ac0d0f0
dc.identifier.uri.none.fl_str_mv	http://hdl.handle.net/10818/33064
dc.identifier.doi.none.fl_str_mv	10.1530/JME-15-0148
identifier_str_mv	Vargas, D., N, Shimokawa., R,,Kaneko., Parra, A., Castellanos, A, N., Lizcano, F.(2016) Regulation of human subcutaneous adipocyte differentiation by EID1, Journal of Molecular Endocrinology (56) 2 113-122 10.1530/JME-15-0148
url	http://jme.endocrinology-journals.org/content/56/2/113.full.pdf+html?sid=339bebb1-5536-46c8-918d-a4485ac0d0f0 http://hdl.handle.net/10818/33064
dc.language.iso.es_CO.fl_str_mv	eng
language	eng
dc.relation.ispartofseries.none.fl_str_mv	Journal of Molecular Endocrinology (2016) 56, 113–122
dc.rights.*.fl_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 International
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dc.publisher.es_CO.fl_str_mv	Journal of Molecular Endocrinology
dc.source.es_CO.fl_str_mv	Universidad de La Sabana Intellectum Repositorio Universidad de La Sabana
institution	Universidad de la Sabana
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Regulation of human subcutaneous adipocyte differentiation by EID1

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