Evaluación de la actividad antibacterial y antifúngica de actinobacterias cultivables aisladas de las riberas del río Guaviare

72 Páginas incluye diagramas

Autores:
Pastrana Camacho, Natalia
Tipo de recurso:
Fecha de publicación:
2015
Institución:
Universidad de la Sabana
Repositorio:
Repositorio Universidad de la Sabana
Idioma:
spa
OAI Identifier:
oai:intellectum.unisabana.edu.co:10818/17433
Acceso en línea:
http://hdl.handle.net/10818/17433
Palabra clave:
Crecimiento bacterial -- Guaviare, Colombia
Resistencia a medicamentos en microorganismos -- Guaviare, Colombia
Bacterias patógenas -- Guaviare, Colombia
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License
Attribution-NonCommercial-NoDerivatives 4.0 International
id REPOUSABA2_97a9e349a97eab343ef62f04f37bbd93
oai_identifier_str oai:intellectum.unisabana.edu.co:10818/17433
network_acronym_str REPOUSABA2
network_name_str Repositorio Universidad de la Sabana
repository_id_str
dc.title.es_CO.fl_str_mv Evaluación de la actividad antibacterial y antifúngica de actinobacterias cultivables aisladas de las riberas del río Guaviare
title Evaluación de la actividad antibacterial y antifúngica de actinobacterias cultivables aisladas de las riberas del río Guaviare
spellingShingle Evaluación de la actividad antibacterial y antifúngica de actinobacterias cultivables aisladas de las riberas del río Guaviare
Crecimiento bacterial -- Guaviare, Colombia
Resistencia a medicamentos en microorganismos -- Guaviare, Colombia
Bacterias patógenas -- Guaviare, Colombia
title_short Evaluación de la actividad antibacterial y antifúngica de actinobacterias cultivables aisladas de las riberas del río Guaviare
title_full Evaluación de la actividad antibacterial y antifúngica de actinobacterias cultivables aisladas de las riberas del río Guaviare
title_fullStr Evaluación de la actividad antibacterial y antifúngica de actinobacterias cultivables aisladas de las riberas del río Guaviare
title_full_unstemmed Evaluación de la actividad antibacterial y antifúngica de actinobacterias cultivables aisladas de las riberas del río Guaviare
title_sort Evaluación de la actividad antibacterial y antifúngica de actinobacterias cultivables aisladas de las riberas del río Guaviare
dc.creator.fl_str_mv Pastrana Camacho, Natalia
dc.contributor.advisor.none.fl_str_mv Díaz Barrera, Luis Eduardo
Suarez, Zulma Rocio
dc.contributor.author.none.fl_str_mv Pastrana Camacho, Natalia
dc.subject.none.fl_str_mv Crecimiento bacterial -- Guaviare, Colombia
Resistencia a medicamentos en microorganismos -- Guaviare, Colombia
Bacterias patógenas -- Guaviare, Colombia
topic Crecimiento bacterial -- Guaviare, Colombia
Resistencia a medicamentos en microorganismos -- Guaviare, Colombia
Bacterias patógenas -- Guaviare, Colombia
description 72 Páginas incluye diagramas
publishDate 2015
dc.date.accessioned.none.fl_str_mv 2015-08-05T22:19:57Z
dc.date.available.none.fl_str_mv 2015-08-05T22:19:57Z
2016-03-03
dc.date.created.none.fl_str_mv 2015-08-04
2015
dc.date.issued.none.fl_str_mv 2015-08-05
dc.type.es_CO.fl_str_mv bachelorThesis
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
dc.type.local.none.fl_str_mv Tesis de especialización
dc.type.hasVersion.none.fl_str_mv publishedVersion
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dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10818/17433
dc.identifier.local.none.fl_str_mv 261047
TE07656
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Dado las recientes investigaciones en el medio acuático que demuestran el potencial bioactivo por parte de estos microorganismos, el presente trabajo se enfocó en la evaluación de la actividad antibacterial y antifúngica de actinobacterias aisladas de agua y sedimentos provenientes del río Guaviare. Las metodologías de tratamiento térmico, enriquecimiento con carbonato de calcio y siembra directa sobre medios de cultivo selectivos fueron utilizadas para el aislamiento de las cepas, donde el enriquecimiento con carbonato de calcio resultó ser el mejor método de aislamiento de actinobacterias, en contraste al tratamiento térmico. Se aislaron 374 bacterias con características morfológicas de actinobacterias en su mayoría provenientes de muestras de sedimentos, a las cuales se les realizó tinción de Gram para corroborar su carácter Gram positivo, pureza y morfología. Una vez purificados, todos los aislamientos fueron conservados en glicerol a -70 ºC y sometidos a pruebas de enfrentamiento directo contra bacterias Gram positivas, Gram negativas y hongos. En total, de los 374 aislamientos 44 cepas inhibieron el crecimiento de B. subtilis, 36 de S. aureus, 15 de E. coli, 10 de A. baumannii y 2 de K. pneumoniae, además 257 presentaron inhibición de quorum sensing al ser evaluadas contra C. violaceum. En cuanto a la actividad antifúngica 76 de los aislamientos inhibieron el crecimiento de T. harzianum, 91 de Fusarium sp. y 150 de C. gloeosporioides. Con base a los resultados iniciales de actividad antimicrobiana, fueron seleccionadas las cepas que presentaron mayor potencial antimicrobiano. Posteriormente, las 7 cepaspaUniversidad de La SabanaEspecialización en Gerencia de Producción y OperacionesEscuela de Ciencias Económicas y AdministrativasAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_abf2Universidad de La SabanaIntellectum Repositorio Universidad de La SabanaCrecimiento bacterial -- Guaviare, ColombiaResistencia a medicamentos en microorganismos -- Guaviare, ColombiaBacterias patógenas -- Guaviare, ColombiaEvaluación de la actividad antibacterial y antifúngica de actinobacterias cultivables aisladas de las riberas del río GuaviarebachelorThesisTesis de especializaciónpublishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_7a1fORIGINALNatalia Pastrana Camacho (tesis).pdfNatalia Pastrana Camacho (tesis).pdfVer documento en PDFapplication/pdf2802217https://intellectum.unisabana.edu.co/bitstream/10818/17433/1/Natalia%20Pastrana%20Camacho%20%20%28tesis%29.pdff50e7ec40763e9c96aa60a3630c6376aMD51Natalia Pastrana Camacho (anexos).pdfNatalia Pastrana Camacho (anexos).pdfVer Anexosapplication/pdf3912296https://intellectum.unisabana.edu.co/bitstream/10818/17433/2/Natalia%20Pastrana%20Camacho%20%20%28anexos%29.pdf099eb912cfb73379c1138d05b33d0a1dMD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81223https://intellectum.unisabana.edu.co/bitstream/10818/17433/3/license_rdf7c9ab7f006165862d8ce9ac5eac01552MD53LICENSElicense.txtlicense.txttext/plain; charset=utf-8498https://intellectum.unisabana.edu.co/bitstream/10818/17433/4/license.txtf52a2cfd4df262e08e9b300d62c85cabMD54Natalia Pastrana Camacho (Carta).pdfNatalia Pastrana Camacho (Carta).pdfapplication/pdf461895https://intellectum.unisabana.edu.co/bitstream/10818/17433/7/Natalia%20Pastrana%20Camacho%20%20%28Carta%29.pdf1e32252948590584a9f22eb6df38934eMD57TEXTNatalia Pastrana Camacho (tesis).pdf.txtNatalia Pastrana Camacho (tesis).pdf.txtExtracted Texttext/plain73https://intellectum.unisabana.edu.co/bitstream/10818/17433/5/Natalia%20Pastrana%20Camacho%20%20%28tesis%29.pdf.txt958e4feee5e80f8367a0d0f2586c3554MD55Natalia Pastrana Camacho (anexos).pdf.txtNatalia Pastrana Camacho (anexos).pdf.txtExtracted Texttext/plain26https://intellectum.unisabana.edu.co/bitstream/10818/17433/6/Natalia%20Pastrana%20Camacho%20%20%28anexos%29.pdf.txt969a2d9af79a1904e58d4b18f0b271feMD5610818/17433oai:intellectum.unisabana.edu.co:10818/174332019-08-11 10:42:12.514Intellectum Universidad de la Sabanacontactointellectum@unisabana.edu.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