Validez diagnóstica de la reacción en cadena de la polimerasa en tiempo real para tuberculosis pleural.

48 Páginas.

Autores:: Casallas Rivera, Martha Alejandra
Cárdenas Bernal, Ana María

Tipo de recurso:

Fecha de publicación:: 2013

Institución:: Universidad de la Sabana

Repositorio:: Repositorio Universidad de la Sabana

Idioma:: spa

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dc.title.es_CO.fl_str_mv	Validez diagnóstica de la reacción en cadena de la polimerasa en tiempo real para tuberculosis pleural.
title	Validez diagnóstica de la reacción en cadena de la polimerasa en tiempo real para tuberculosis pleural.
spellingShingle	Validez diagnóstica de la reacción en cadena de la polimerasa en tiempo real para tuberculosis pleural. Biología molecular - Investigaciones Tuberculosis - Investigaciones Derrame pleural - Investigaciones Granulometría - Investigaciones Mycobacterium tuberculosis - Investigaciones
title_short	Validez diagnóstica de la reacción en cadena de la polimerasa en tiempo real para tuberculosis pleural.
title_full	Validez diagnóstica de la reacción en cadena de la polimerasa en tiempo real para tuberculosis pleural.
title_fullStr	Validez diagnóstica de la reacción en cadena de la polimerasa en tiempo real para tuberculosis pleural.
title_full_unstemmed	Validez diagnóstica de la reacción en cadena de la polimerasa en tiempo real para tuberculosis pleural.
title_sort	Validez diagnóstica de la reacción en cadena de la polimerasa en tiempo real para tuberculosis pleural.
dc.creator.fl_str_mv	Casallas Rivera, Martha Alejandra Cárdenas Bernal, Ana María
dc.contributor.advisor.none.fl_str_mv	Giraldo Cadavid, Luis Fernando
dc.contributor.author.none.fl_str_mv	Casallas Rivera, Martha Alejandra Cárdenas Bernal, Ana María
dc.subject.es_CO.fl_str_mv	Biología molecular - Investigaciones Tuberculosis - Investigaciones Derrame pleural - Investigaciones Granulometría - Investigaciones Mycobacterium tuberculosis - Investigaciones
topic	Biología molecular - Investigaciones Tuberculosis - Investigaciones Derrame pleural - Investigaciones Granulometría - Investigaciones Mycobacterium tuberculosis - Investigaciones
description	48 Páginas.
publishDate	2013
dc.date.accessioned.none.fl_str_mv	2013-06-27T13:04:28Z
dc.date.available.none.fl_str_mv	2013-06-27T13:04:28Z
dc.date.created.none.fl_str_mv	2013-06-27
dc.date.issued.none.fl_str_mv	2013
dc.type.none.fl_str_mv	bachelorThesis
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dc.type.local.none.fl_str_mv	Tesis de especialización
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dc.identifier.citation.none.fl_str_mv	Lamsal M, Gautam N, Bhatta N, Majhi S, Baral N, Bhattacharya SK. Diagnostic utility of adenosine deaminase (ADA) activity in pleural fluid and serum of tuberculous and non-tuberculous respiratory disease patients. Southeast Asian J Trop Med Public Health. 2007 Mar;38(2):363-9. Vélez H RW, Borrero J, Restrepo J, Torres C, Awad C, Maldonado D y Chaparro C. . NEUMOLOGÍA. Fundamentos de Medicina. edición, editor. Medellín: Corporación para Investigaciones Biológicas; 2007. Salud OPdl. “En el 2010, se estiman 11.200 casos de tuberculosis en Colombia”. 2010; Available from: http://new.paho.org/col/index.php?option=com_content&task=view&id=1022&Itemid=469. GP KPaK. Public health mycobacteriology: a guide for the level III laboratory. Atlanta: U.S. Department of Health and Human Services, Centers for Disease Control.; 1985 Trajman A, Pai M, Dheda K, van Zyl Smit R, Zwerling AA, Joshi R, et al. Novel tests for diagnosing tuberculous pleural effusion: what works and what does not? Eur Respir J. 2008 May;31(5):1098-106. Wallis RS, Pai M, Menzies D, Doherty TM, Walzl G, Perkins MD, et al. Biomarkers and diagnostics for tuberculosis: progress, needs, and translation into practice. Lancet. 2010 May 29;375(9729):1920-37. Kunnath-Velayudhan S, Gennaro ML. Immunodiagnosis of tuberculosis: a dynamic view of biomarker discovery. Clin Microbiol Rev. 2011 Oct;24(4):792-805. Villena V, Lopez-Encuentra A, Pozo F, Echave-Sustaeta J, Ortuno-de-Solo B, Estenoz-Alfaro J, et al. Interferon gamma levels in pleural fluid for the diagnosis of tuberculosis. Am J Med. 2003 Oct 1;115(5):365-70. K. S. Escenario de la técnica de la reacción en cadena de la polimerasa como prueba diagnóstica en tuberculosis. Gac Méd Caracas. 2009;117:6. Organization WH. Global tuberculosis control: surveillance, planning, financing. Geneva2006. Jimenez Castro D, Diaz Nuevo G, Perez-Rodriguez E, Light RW. Diagnostic value of adenosine deaminase in nontuberculous lymphocytic pleural effusions. Eur Respir J. 2003 Feb;21(2):220-4 pública IndsSdvyces. Boletín Epidemiológico semanal. 2012. Lancelot M. Pinto JG, Samuel G. Schumacher, Claudia M. Denkinger, Karen R. Steingart, Madhukar Pai. Immunodiagnosis of Tuberculosis: State of the Art. Med Princ Pract 2012. [Review]. 2011;21:10 Villegas MV, Labrada LA, Saravia NG. Evaluation of polymerase chain reaction, adenosine deaminase, and interferon-gamma in pleural fluid for the differential diagnosis of pleural tuberculosis. Chest. 2000 Nov;118(5):1355-64. Light RW. Clinical practice. Pleural effusion. N Engl J Med. 2002 Jun 20;346(25):1971-7. Palomino JC LSaRV. Tuberculosis 2007. primera edición ed2007. JM. P. Tuberculous pleural effusion. Lung. 2009;187(5):8. Moudgil H, Sridhar G, Leitch AG. Reactivation disease: the commonest form of tuberculous pleural effusion in Edinburgh, 1980-1991. Respir Med. 1994 Apr;88(4):301-4. Stead WW, Eichenholz A, Stauss HK. Operative and pathologic findings in twenty-four patients with syndrome of idiopathic pleurisy with effusion, presumably tuberculous. Am Rev Tuberc. 1955 Apr;71(4):473-502 RW. L. Pleural Diseases. Quinta edición ed. Baltimore: Lippincott Williams and Wilkins; 2007. Levine H, Szanto PB, Cugell DW. Tuberculous pleurisy. An acute illness. Arch Intern Med. 1968 Oct;122(4):329-32. JK SHaM. Laboratory diagnosis of the mycobacterioses. American Society for Microbiology. 1983 Scharer L, McClement JH. Isolation of tubercle bacilli from needle biopsy specimens of parietal pleura. Am Rev Respir Dis. 1968 Mar;97(3):466-8. Udwadia ZF, Sen T. Pleural tuberculosis: an update. Curr Opin Pulm Med. 2010 Jul;16(4):399-406. Villena Garrido V FSJ, Hernández Blasco L, de Pablo Gafas A, Pérez Rodríguez E, Rodríguez Panadero F, Romero Candeira S, Salvatierra Velázquez A, Valdés Cuadrado L. [Diagnosis and treatment of pleural effusion]. Arch Bronconeumol. 2006;42(7):24 . J. C. Reacción en cadena de la polimerasa (PCR) en tiempo real. Enferm Infecc Microbiol Clin. 2004;22:6. Parashar D, Chauhan DS, Sharma VD, Katoch VM. Applications of real-time PCR technology to mycobacterial research. Indian J Med Res. 2006 Oct;124(4):385-98 Shain EB, Clemens JM. A new method for robust quantitative and qualitative analysis of real-time PCR. Nucleic Acids Res. 2008 Aug;36(14):e91. Pai M, Flores LL, Hubbard A, Riley LW, Colford JM, Jr. Nucleic acid amplification tests in the diagnosis of tuberculous pleuritis: a systematic review and meta-analysis. BMC Infect Dis. 2004 Feb 23;4:6. Chakravorty S, Sen MK, Tyagi JS. Diagnosis of extrapulmonary tuberculosis by smear, culture, and PCR using universal sample processing technology. J Clin Microbiol. 2005 Sep;43(9):4357-62. Liu KT, Su WJ, Perng RP. Clinical utility of polymerase chain reaction for diagnosis of smearnegative pleural tuberculosis. J Chin Med Assoc. 2007 Apr;70(4):148-51; discussion 6-7 Kalantri Y, Hemvani N, Chitnis DS. Evaluation of real-time polymerase chain reaction, interferon-gamma, adenosine deaminase, and immunoglobulin A for the efficient diagnosis of pleural tuberculosis. Int J Infect Dis. 2011 Apr;15(4):e226-31. David Machin MJC, Say Beng Tan, and, Tan SH. Sample Size Tables for Clinical Studies. Tercera edición ed: Blackwell Publishing; 2009. Li J, Fine J. On sample size for sensitivity and specificity in prospective diagnostic accuracy studies. Stat Med. 2004 Aug 30;23(16):2537-50.
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identifier_str_mv	Lamsal M, Gautam N, Bhatta N, Majhi S, Baral N, Bhattacharya SK. Diagnostic utility of adenosine deaminase (ADA) activity in pleural fluid and serum of tuberculous and non-tuberculous respiratory disease patients. Southeast Asian J Trop Med Public Health. 2007 Mar;38(2):363-9. Vélez H RW, Borrero J, Restrepo J, Torres C, Awad C, Maldonado D y Chaparro C. . NEUMOLOGÍA. Fundamentos de Medicina. edición, editor. Medellín: Corporación para Investigaciones Biológicas; 2007. Salud OPdl. “En el 2010, se estiman 11.200 casos de tuberculosis en Colombia”. 2010; Available from: http://new.paho.org/col/index.php?option=com_content&task=view&id=1022&Itemid=469. GP KPaK. Public health mycobacteriology: a guide for the level III laboratory. Atlanta: U.S. Department of Health and Human Services, Centers for Disease Control.; 1985 Trajman A, Pai M, Dheda K, van Zyl Smit R, Zwerling AA, Joshi R, et al. Novel tests for diagnosing tuberculous pleural effusion: what works and what does not? Eur Respir J. 2008 May;31(5):1098-106. Wallis RS, Pai M, Menzies D, Doherty TM, Walzl G, Perkins MD, et al. Biomarkers and diagnostics for tuberculosis: progress, needs, and translation into practice. Lancet. 2010 May 29;375(9729):1920-37. Kunnath-Velayudhan S, Gennaro ML. Immunodiagnosis of tuberculosis: a dynamic view of biomarker discovery. Clin Microbiol Rev. 2011 Oct;24(4):792-805. Villena V, Lopez-Encuentra A, Pozo F, Echave-Sustaeta J, Ortuno-de-Solo B, Estenoz-Alfaro J, et al. Interferon gamma levels in pleural fluid for the diagnosis of tuberculosis. Am J Med. 2003 Oct 1;115(5):365-70. K. S. Escenario de la técnica de la reacción en cadena de la polimerasa como prueba diagnóstica en tuberculosis. Gac Méd Caracas. 2009;117:6. Organization WH. Global tuberculosis control: surveillance, planning, financing. Geneva2006. Jimenez Castro D, Diaz Nuevo G, Perez-Rodriguez E, Light RW. Diagnostic value of adenosine deaminase in nontuberculous lymphocytic pleural effusions. Eur Respir J. 2003 Feb;21(2):220-4 pública IndsSdvyces. Boletín Epidemiológico semanal. 2012. Lancelot M. Pinto JG, Samuel G. Schumacher, Claudia M. Denkinger, Karen R. Steingart, Madhukar Pai. Immunodiagnosis of Tuberculosis: State of the Art. Med Princ Pract 2012. [Review]. 2011;21:10 Villegas MV, Labrada LA, Saravia NG. Evaluation of polymerase chain reaction, adenosine deaminase, and interferon-gamma in pleural fluid for the differential diagnosis of pleural tuberculosis. Chest. 2000 Nov;118(5):1355-64. Light RW. Clinical practice. Pleural effusion. N Engl J Med. 2002 Jun 20;346(25):1971-7. Palomino JC LSaRV. Tuberculosis 2007. primera edición ed2007. JM. P. Tuberculous pleural effusion. Lung. 2009;187(5):8. Moudgil H, Sridhar G, Leitch AG. Reactivation disease: the commonest form of tuberculous pleural effusion in Edinburgh, 1980-1991. Respir Med. 1994 Apr;88(4):301-4. Stead WW, Eichenholz A, Stauss HK. Operative and pathologic findings in twenty-four patients with syndrome of idiopathic pleurisy with effusion, presumably tuberculous. Am Rev Tuberc. 1955 Apr;71(4):473-502 RW. L. Pleural Diseases. Quinta edición ed. Baltimore: Lippincott Williams and Wilkins; 2007. Levine H, Szanto PB, Cugell DW. Tuberculous pleurisy. An acute illness. Arch Intern Med. 1968 Oct;122(4):329-32. JK SHaM. Laboratory diagnosis of the mycobacterioses. American Society for Microbiology. 1983 Scharer L, McClement JH. Isolation of tubercle bacilli from needle biopsy specimens of parietal pleura. Am Rev Respir Dis. 1968 Mar;97(3):466-8. Udwadia ZF, Sen T. Pleural tuberculosis: an update. Curr Opin Pulm Med. 2010 Jul;16(4):399-406. Villena Garrido V FSJ, Hernández Blasco L, de Pablo Gafas A, Pérez Rodríguez E, Rodríguez Panadero F, Romero Candeira S, Salvatierra Velázquez A, Valdés Cuadrado L. [Diagnosis and treatment of pleural effusion]. Arch Bronconeumol. 2006;42(7):24 . J. C. Reacción en cadena de la polimerasa (PCR) en tiempo real. Enferm Infecc Microbiol Clin. 2004;22:6. Parashar D, Chauhan DS, Sharma VD, Katoch VM. Applications of real-time PCR technology to mycobacterial research. Indian J Med Res. 2006 Oct;124(4):385-98 Shain EB, Clemens JM. A new method for robust quantitative and qualitative analysis of real-time PCR. Nucleic Acids Res. 2008 Aug;36(14):e91. Pai M, Flores LL, Hubbard A, Riley LW, Colford JM, Jr. Nucleic acid amplification tests in the diagnosis of tuberculous pleuritis: a systematic review and meta-analysis. BMC Infect Dis. 2004 Feb 23;4:6. Chakravorty S, Sen MK, Tyagi JS. Diagnosis of extrapulmonary tuberculosis by smear, culture, and PCR using universal sample processing technology. J Clin Microbiol. 2005 Sep;43(9):4357-62. Liu KT, Su WJ, Perng RP. Clinical utility of polymerase chain reaction for diagnosis of smearnegative pleural tuberculosis. J Chin Med Assoc. 2007 Apr;70(4):148-51; discussion 6-7 Kalantri Y, Hemvani N, Chitnis DS. Evaluation of real-time polymerase chain reaction, interferon-gamma, adenosine deaminase, and immunoglobulin A for the efficient diagnosis of pleural tuberculosis. Int J Infect Dis. 2011 Apr;15(4):e226-31. David Machin MJC, Say Beng Tan, and, Tan SH. Sample Size Tables for Clinical Studies. Tercera edición ed: Blackwell Publishing; 2009. Li J, Fine J. On sample size for sensitivity and specificity in prospective diagnostic accuracy studies. Stat Med. 2004 Aug 30;23(16):2537-50. 254659 TE05949
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dc.publisher.program.none.fl_str_mv	Especialización en Medicina Interna
dc.publisher.department.none.fl_str_mv	Facultad de Medicina
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spelling	Giraldo Cadavid, Luis FernandoCasallas Rivera, Martha AlejandraCárdenas Bernal, Ana MaríaEspecialista en Medicina Interna2013-06-27T13:04:28Z2013-06-27T13:04:28Z2013-06-272013Lamsal M, Gautam N, Bhatta N, Majhi S, Baral N, Bhattacharya SK. Diagnostic utility of adenosine deaminase (ADA) activity in pleural fluid and serum of tuberculous and non-tuberculous respiratory disease patients. Southeast Asian J Trop Med Public Health. 2007 Mar;38(2):363-9.Vélez H RW, Borrero J, Restrepo J, Torres C, Awad C, Maldonado D y Chaparro C. . NEUMOLOGÍA. Fundamentos de Medicina. edición, editor. Medellín: Corporación para Investigaciones Biológicas; 2007.Salud OPdl. “En el 2010, se estiman 11.200 casos de tuberculosis en Colombia”. 2010; Available from: http://new.paho.org/col/index.php?option=com_content&task=view&id=1022&Itemid=469.GP KPaK. Public health mycobacteriology: a guide for the level III laboratory. Atlanta: U.S. Department of Health and Human Services, Centers for Disease Control.; 1985Trajman A, Pai M, Dheda K, van Zyl Smit R, Zwerling AA, Joshi R, et al. Novel tests for diagnosing tuberculous pleural effusion: what works and what does not? Eur Respir J. 2008 May;31(5):1098-106.Wallis RS, Pai M, Menzies D, Doherty TM, Walzl G, Perkins MD, et al. Biomarkers and diagnostics for tuberculosis: progress, needs, and translation into practice. Lancet. 2010 May 29;375(9729):1920-37.Kunnath-Velayudhan S, Gennaro ML. Immunodiagnosis of tuberculosis: a dynamic view of biomarker discovery. Clin Microbiol Rev. 2011 Oct;24(4):792-805.Villena V, Lopez-Encuentra A, Pozo F, Echave-Sustaeta J, Ortuno-de-Solo B, Estenoz-Alfaro J, et al. Interferon gamma levels in pleural fluid for the diagnosis of tuberculosis. Am J Med. 2003 Oct 1;115(5):365-70.K. S. Escenario de la técnica de la reacción en cadena de la polimerasa como prueba diagnóstica en tuberculosis. Gac Méd Caracas. 2009;117:6.Organization WH. Global tuberculosis control: surveillance, planning, financing. Geneva2006.Jimenez Castro D, Diaz Nuevo G, Perez-Rodriguez E, Light RW. Diagnostic value of adenosine deaminase in nontuberculous lymphocytic pleural effusions. Eur Respir J. 2003 Feb;21(2):220-4pública IndsSdvyces. Boletín Epidemiológico semanal. 2012.Lancelot M. Pinto JG, Samuel G. Schumacher, Claudia M. Denkinger, Karen R. Steingart, Madhukar Pai. Immunodiagnosis of Tuberculosis: State of the Art. Med Princ Pract 2012. [Review]. 2011;21:10Villegas MV, Labrada LA, Saravia NG. Evaluation of polymerase chain reaction, adenosine deaminase, and interferon-gamma in pleural fluid for the differential diagnosis of pleural tuberculosis. Chest. 2000 Nov;118(5):1355-64.Light RW. Clinical practice. Pleural effusion. N Engl J Med. 2002 Jun 20;346(25):1971-7.Palomino JC LSaRV. Tuberculosis 2007. primera edición ed2007.JM. P. Tuberculous pleural effusion. Lung. 2009;187(5):8.Moudgil H, Sridhar G, Leitch AG. Reactivation disease: the commonest form of tuberculous pleural effusion in Edinburgh, 1980-1991. Respir Med. 1994 Apr;88(4):301-4.Stead WW, Eichenholz A, Stauss HK. Operative and pathologic findings in twenty-four patients with syndrome of idiopathic pleurisy with effusion, presumably tuberculous. Am Rev Tuberc. 1955 Apr;71(4):473-502RW. L. Pleural Diseases. Quinta edición ed. Baltimore: Lippincott Williams and Wilkins; 2007.Levine H, Szanto PB, Cugell DW. Tuberculous pleurisy. An acute illness. Arch Intern Med. 1968 Oct;122(4):329-32.JK SHaM. Laboratory diagnosis of the mycobacterioses. American Society for Microbiology. 1983Scharer L, McClement JH. Isolation of tubercle bacilli from needle biopsy specimens of parietal pleura. Am Rev Respir Dis. 1968 Mar;97(3):466-8.Udwadia ZF, Sen T. Pleural tuberculosis: an update. Curr Opin Pulm Med. 2010 Jul;16(4):399-406.Villena Garrido V FSJ, Hernández Blasco L, de Pablo Gafas A, Pérez Rodríguez E, Rodríguez Panadero F, Romero Candeira S, Salvatierra Velázquez A, Valdés Cuadrado L. [Diagnosis and treatment of pleural effusion]. Arch Bronconeumol. 2006;42(7):24. J. C. Reacción en cadena de la polimerasa (PCR) en tiempo real. Enferm Infecc Microbiol Clin. 2004;22:6.Parashar D, Chauhan DS, Sharma VD, Katoch VM. Applications of real-time PCR technology to mycobacterial research. Indian J Med Res. 2006 Oct;124(4):385-98Shain EB, Clemens JM. A new method for robust quantitative and qualitative analysis of real-time PCR. Nucleic Acids Res. 2008 Aug;36(14):e91.Pai M, Flores LL, Hubbard A, Riley LW, Colford JM, Jr. Nucleic acid amplification tests in the diagnosis of tuberculous pleuritis: a systematic review and meta-analysis. BMC Infect Dis. 2004 Feb 23;4:6.Chakravorty S, Sen MK, Tyagi JS. Diagnosis of extrapulmonary tuberculosis by smear, culture, and PCR using universal sample processing technology. J Clin Microbiol. 2005 Sep;43(9):4357-62.Liu KT, Su WJ, Perng RP. Clinical utility of polymerase chain reaction for diagnosis of smearnegative pleural tuberculosis. J Chin Med Assoc. 2007 Apr;70(4):148-51; discussion 6-7Kalantri Y, Hemvani N, Chitnis DS. Evaluation of real-time polymerase chain reaction, interferon-gamma, adenosine deaminase, and immunoglobulin A for the efficient diagnosis of pleural tuberculosis. Int J Infect Dis. 2011 Apr;15(4):e226-31.David Machin MJC, Say Beng Tan, and, Tan SH. Sample Size Tables for Clinical Studies. Tercera edición ed: Blackwell Publishing; 2009.Li J, Fine J. On sample size for sensitivity and specificity in prospective diagnostic accuracy studies. Stat Med. 2004 Aug 30;23(16):2537-50.http://hdl.handle.net/10818/7877254659TE0594948 Páginas.Para el diagnóstico de tuberculosis pleural (TBP) el patrón de oro es invasivo y demorado. Métodos. Validez de la RT – PCR comparada con un patrón de oro compuesto. Incluimos pacientes con derrame pleural tipo exudado linfocitario. Se consideró diagnóstico de TBP si el paciente tenía mínimo una de tres pruebas positiva: Zielh-Neelsen o cultivo para MTb en líquido o tejido pleural o biopsia pleural; se descartó TBP si las tres pruebas eran negativas. Resultados. RT – PCR mostró una sensibilidad del 66,7%, una especificidad del 93,5%, un VPP de 75% y un VPN de 90,6%. Conclusiones. La RT - PCR para TBP no es un método diagnóstico definitivo, permite definir rápidamente el manejo, mientras llegan reportes de pruebas diagnósticas definitivas.spaUniversidad de la SabanaEspecialización en Medicina InternaFacultad de MedicinaUniversidad de La SabanaIntellectum Repositorio Universidad de La SabanaBiología molecular - InvestigacionesTuberculosis - InvestigacionesDerrame pleural - InvestigacionesGranulometría - InvestigacionesMycobacterium tuberculosis - InvestigacionesValidez diagnóstica de la reacción en cadena de la polimerasa en tiempo real para tuberculosis pleural.bachelorThesisTesis de especializaciónpublishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/access_right/c_16ecORIGINALMARTHA ALEJANDRA CASALLAS_254659 FINAL.pdfMARTHA ALEJANDRA CASALLAS_254659 FINAL.pdfVer documento en PDFapplication/pdf674266https://intellectum.unisabana.edu.co/bitstream/10818/7877/3/MARTHA%20ALEJANDRA%20CASALLAS_254659%20FINAL.pdf9c708f9bc943db379a17ef55ea577a08MD53LICENSElicense.txtlicense.txttext/plain; charset=utf-8498https://intellectum.unisabana.edu.co/bitstream/10818/7877/2/license.txtf52a2cfd4df262e08e9b300d62c85cabMD52Martha Alejandra Casallas Rivera (Tesis).pdfMartha Alejandra Casallas Rivera (Tesis).pdfapplication/pdf1140803https://intellectum.unisabana.edu.co/bitstream/10818/7877/5/Martha%20Alejandra%20Casallas%20Rivera%20%28Tesis%29.pdf8757def9bbe2e6bc8b6c258811937f22MD55TEXTMARTHA ALEJANDRA CASALLAS_254659 FINAL.pdf.txtMARTHA ALEJANDRA CASALLAS_254659 FINAL.pdf.txtExtracted Texttext/plain50https://intellectum.unisabana.edu.co/bitstream/10818/7877/4/MARTHA%20ALEJANDRA%20CASALLAS_254659%20FINAL.pdf.txtb6997699b324cbe5385f0ceed53d740aMD5410818/7877oai:intellectum.unisabana.edu.co:10818/78772019-08-13 16:26:37.767Intellectum Universidad de la Sabanacontactointellectum@unisabana.edu.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

Validez diagnóstica de la reacción en cadena de la polimerasa en tiempo real para tuberculosis pleural.

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