Highly Sensitive Detection and Genotyping of HPV by PCR Multiplex and Luminex Technology in a Cohort of Colombian Women with Abnormal Cytology

10 páginas

Autores:: García Robayo, Dabeiba Adriana
Cid-Arregui, Ángel
Schmitt, Markus
Castillo Zamora, Marcos Fidel
Briceño Balcázar, Ignacio
Aristizábal Gutiérrez, Fabio Ancízar

Tipo de recurso:

Fecha de publicación:: 2011

Institución:: Universidad de la Sabana

Repositorio:: Repositorio Universidad de la Sabana

Idioma:: eng

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spelling	García Robayo, Dabeiba AdrianaCid-Arregui, ÁngelSchmitt, MarkusCastillo Zamora, Marcos FidelBriceño Balcázar, IgnacioAristizábal Gutiérrez, Fabio Ancízar10/14/2016 12:042016-10-14T17:04:04Z20111874-3579https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3134977/http://hdl.handle.net/10818/2753110.2174/187435790110501007010 páginasCancer of the uterine cervix (CC) is the second most common cancer in women worldwide. In Colombia, CC is the second most frequent cancer among the entire women population and the first among women aged between 15 and 44 years, with an estimated incidence of 24.9 cases/100,000 inhabitants. The main risk factor is infection with one or more high-risk human papillomavirus (HPV) types. The aim of this study was to estimate the genotype-specific prevalence of human papillomavirus (HPV) DNA in patients with cervical pathology using the multiplex PCR and Luminex xMAP technology. In addition, we compared genotyping with Luminex xMAP and with Reverse Line Blot (RLB). A cohort of 160 patients participated in the study, of which 25.6% had no cervical lesions, 35% presented cervical intraepithelial neoplasia of grade I (CIN I), 10% CIN II, 20.6% CIN III and 8.8% CC. The most frequent viral types in all lesion grades were HPV16 and HPV18. Infections by a unique virus were less frequent (19.4%) than multiple infections (80.6%). Single infections were found in 22% of women with no cervical lesions, and in 14.3% of CIN I, 18.7% CIN II, 21.2% CIN III and 28.6% of CC. Multiple infections were observed in 78.0% of cervical samples with negative histopathologic diagnosis, and in 85.7% of CIN I, 81.2% CIN II, 78.8% CIN III and 71.4% CC. All samples analyzed with Luminex xMAP were HPV-positive, while we could detect HPV in only 48.8% of cases with RLB. Of the samples positive by both methods, there was a 67.2% correlation in the viral type(s) detected. In conclusion, Luminex suspension array showed a remarkably higher sensitivity compared with RLB. Multiple infections were unexpectedly common, being HPV types 16 and 18 the most prevalent in all histopathologic grades.application/pdfengThe Open Virology JournalOpen Virol J. 2011; 5: 70–79Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_abf2Universidad de La SabanaIntellectum Repositorio Universidad de La SabanaHuman papillomavirus (HPV)CINCervical cancerReverse line blot (RLB)Luminex xMAPPap smearAbnormal cytologyHuman papillomavirus (HPV)CINLuminex xMAPHighly Sensitive Detection and Genotyping of HPV by PCR Multiplex and Luminex Technology in a Cohort of Colombian Women with Abnormal CytologyarticlepublishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501LICENSElicense.txtlicense.txttext/plain; charset=utf-8498https://intellectum.unisabana.edu.co/bitstream/10818/27531/3/license.txtf52a2cfd4df262e08e9b300d62c85cabMD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81223https://intellectum.unisabana.edu.co/bitstream/10818/27531/2/license_rdf7c9ab7f006165862d8ce9ac5eac01552MD5210818/27531oai:intellectum.unisabana.edu.co:10818/275312022-05-10 05:17:21.877Intellectum Universidad de la Sabanacontactointellectum@unisabana.edu.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
dc.title.es_CO.fl_str_mv	Highly Sensitive Detection and Genotyping of HPV by PCR Multiplex and Luminex Technology in a Cohort of Colombian Women with Abnormal Cytology
title	Highly Sensitive Detection and Genotyping of HPV by PCR Multiplex and Luminex Technology in a Cohort of Colombian Women with Abnormal Cytology
spellingShingle	Highly Sensitive Detection and Genotyping of HPV by PCR Multiplex and Luminex Technology in a Cohort of Colombian Women with Abnormal Cytology Human papillomavirus (HPV) CIN Cervical cancer Reverse line blot (RLB) Luminex xMAP Pap smear Abnormal cytology Human papillomavirus (HPV) CIN Luminex xMAP
title_short	Highly Sensitive Detection and Genotyping of HPV by PCR Multiplex and Luminex Technology in a Cohort of Colombian Women with Abnormal Cytology
title_full	Highly Sensitive Detection and Genotyping of HPV by PCR Multiplex and Luminex Technology in a Cohort of Colombian Women with Abnormal Cytology
title_fullStr	Highly Sensitive Detection and Genotyping of HPV by PCR Multiplex and Luminex Technology in a Cohort of Colombian Women with Abnormal Cytology
title_full_unstemmed	Highly Sensitive Detection and Genotyping of HPV by PCR Multiplex and Luminex Technology in a Cohort of Colombian Women with Abnormal Cytology
title_sort	Highly Sensitive Detection and Genotyping of HPV by PCR Multiplex and Luminex Technology in a Cohort of Colombian Women with Abnormal Cytology
dc.creator.fl_str_mv	García Robayo, Dabeiba Adriana Cid-Arregui, Ángel Schmitt, Markus Castillo Zamora, Marcos Fidel Briceño Balcázar, Ignacio Aristizábal Gutiérrez, Fabio Ancízar
dc.contributor.author.none.fl_str_mv	García Robayo, Dabeiba Adriana Cid-Arregui, Ángel Schmitt, Markus Castillo Zamora, Marcos Fidel Briceño Balcázar, Ignacio Aristizábal Gutiérrez, Fabio Ancízar
dc.subject.none.fl_str_mv	Human papillomavirus (HPV) CIN Cervical cancer Reverse line blot (RLB) Luminex xMAP Pap smear Abnormal cytology
topic	Human papillomavirus (HPV) CIN Cervical cancer Reverse line blot (RLB) Luminex xMAP Pap smear Abnormal cytology Human papillomavirus (HPV) CIN Luminex xMAP
dc.subject.other.en.fl_str_mv	Human papillomavirus (HPV) CIN Luminex xMAP
description	10 páginas
publishDate	2011
dc.date.accessioned.none.fl_str_mv	10/14/2016 12:04
dc.date.issued.none.fl_str_mv	2011
dc.date.available.none.fl_str_mv	2016-10-14T17:04:04Z
dc.type.en.fl_str_mv	article
dc.type.coarversion.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv	http://purl.org/coar/resource_type/c_6501
dc.type.hasVersion.es_CO.fl_str_mv	publishedVersion
dc.identifier.issn.none.fl_str_mv	1874-3579
dc.identifier.other.none.fl_str_mv	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3134977/
dc.identifier.uri.none.fl_str_mv	http://hdl.handle.net/10818/27531
dc.identifier.doi.none.fl_str_mv	10.2174/1874357901105010070
identifier_str_mv	1874-3579 10.2174/1874357901105010070
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3134977/ http://hdl.handle.net/10818/27531
dc.language.iso.es_CO.fl_str_mv	eng
language	eng
dc.relation.ispartofseries.none.fl_str_mv	Open Virol J. 2011; 5: 70–79
dc.rights.*.fl_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.coar.fl_str_mv	http://purl.org/coar/access_right/c_abf2
dc.rights.uri.*.fl_str_mv	http://creativecommons.org/licenses/by-nc-nd/4.0/
rights_invalid_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_abf2
dc.format.es_CO.fl_str_mv	application/pdf
dc.publisher.es_CO.fl_str_mv	The Open Virology Journal
dc.source.es_CO.fl_str_mv	Universidad de La Sabana Intellectum Repositorio Universidad de La Sabana
institution	Universidad de la Sabana
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Highly Sensitive Detection and Genotyping of HPV by PCR Multiplex and Luminex Technology in a Cohort of Colombian Women with Abnormal Cytology

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