Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor
16 páginas
- Autores:
-
Bustos, Rosa Helena
Zapata, Carlos
Esteban Mancera, Efraín Arsenio
García Casallas, Julio César
Jáuregui, Edwin
- Tipo de recurso:
- Fecha de publicación:
- 2018
- Institución:
- Universidad de la Sabana
- Repositorio:
- Repositorio Universidad de la Sabana
- Idioma:
- eng
- OAI Identifier:
- oai:intellectum.unisabana.edu.co:10818/43543
- Acceso en línea:
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876701/
http://hdl.handle.net/10818/43543
- Palabra clave:
- Biological drug
Therapeutic drug monitoring (TDM)
Optical biosensor
- Rights
- License
- Attribution-NonCommercial-NoDerivatives 4.0 International
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Bustos, Rosa HelenaZapata, CarlosEsteban Mancera, Efraín ArsenioGarcía Casallas, Julio CésarJáuregui, Edwin10/5/2020 10:362020-10-05T15:36:40Z2018-02-261424-8220https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876701/http://hdl.handle.net/10818/4354310.3390/s1803069116 páginasThis study describes the development of an immunosensory label-free quantification methodology based on surface plasmon resonance (SPR) and its applicability in measuring/evaluating therapeutic drug monitoring (TDM) of anti-TNF-α monoclonal antibody (adalimumab) in rheumatoid arthritis (RA) patients. The experimental parameters evaluated in this study were immobilising ligands by pre-concentration assays, sensor surface regeneration, ascertaining the method’s sensitivity and correlating the results from quantifying plasma samples by ELISA immunoassay. The results showed that TNF-α quantification values (in RU) were significantly different when comparing patients (~50–250 RU) to controls (~10–20 RU). Likewise, there was 0.97 correlation for patients and 0.91 for healthy volunteers using SPR and ELISA comparison methodologies. SPR immunosensory detection provided a precise, sensitive strategy, along with real-time determination, for quantifying adalimumab, having great potential for clinical routine regarding TDM.application/pdfengSensors (Basel)Sensors (Basel). 2018 Mar;18(3): 691Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_abf2Universidad de La SabanaIntellectum Repositorio Universidad de La SabanaBiological drugTherapeutic drug monitoring (TDM)Optical biosensorLabel-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical BiosensorarticlepublishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805https://intellectum.unisabana.edu.co/bitstream/10818/43543/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-8498https://intellectum.unisabana.edu.co/bitstream/10818/43543/3/license.txtf52a2cfd4df262e08e9b300d62c85cabMD5310818/43543oai:intellectum.unisabana.edu.co:10818/435432022-05-10 05:20:19.119Intellectum Universidad de la Sabanacontactointellectum@unisabana.edu.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 |
| dc.title.es_CO.fl_str_mv |
Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor |
| title |
Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor |
| spellingShingle |
Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor Biological drug Therapeutic drug monitoring (TDM) Optical biosensor |
| title_short |
Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor |
| title_full |
Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor |
| title_fullStr |
Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor |
| title_full_unstemmed |
Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor |
| title_sort |
Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor |
| dc.creator.fl_str_mv |
Bustos, Rosa Helena Zapata, Carlos Esteban Mancera, Efraín Arsenio García Casallas, Julio César Jáuregui, Edwin |
| dc.contributor.author.none.fl_str_mv |
Bustos, Rosa Helena Zapata, Carlos Esteban Mancera, Efraín Arsenio García Casallas, Julio César Jáuregui, Edwin |
| dc.subject.es_CO.fl_str_mv |
Biological drug Therapeutic drug monitoring (TDM) Optical biosensor |
| topic |
Biological drug Therapeutic drug monitoring (TDM) Optical biosensor |
| description |
16 páginas |
| publishDate |
2018 |
| dc.date.accessioned.none.fl_str_mv |
10/5/2020 10:36 |
| dc.date.issued.none.fl_str_mv |
2018-02-26 |
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2020-10-05T15:36:40Z |
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article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
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publishedVersion |
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1424-8220 |
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876701/ |
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http://hdl.handle.net/10818/43543 |
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10.3390/s18030691 |
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1424-8220 10.3390/s18030691 |
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876701/ http://hdl.handle.net/10818/43543 |
| dc.language.iso.es_CO.fl_str_mv |
eng |
| language |
eng |
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Sensors (Basel). 2018 Mar;18(3): 691 |
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Attribution-NonCommercial-NoDerivatives 4.0 International |
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http://purl.org/coar/access_right/c_abf2 |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_abf2 |
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application/pdf |
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Sensors (Basel) |
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Universidad de La Sabana Intellectum Repositorio Universidad de La Sabana |
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Universidad de la Sabana |
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