Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima
In the department of Tolima, there are no studies that allow us to specify with certainty the magnitude of the ophidic accident caused by Crotalus durissus; Therefore, there is a need to generate toxicological information, as well as knowledge of the potential antimicrobial potential of molecules su...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2020
- Institución:
- Universidad Pedagógica y Tecnológica de Colombia
- Repositorio:
- RiUPTC: Repositorio Institucional UPTC
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uptc.edu.co:001/15279
- Acceso en línea:
- https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9869
https://repositorio.uptc.edu.co/handle/001/15279
- Palabra clave:
- Actividad bactericida
Cascabel
Crotalus durissus
Fosfolipasas
Hemolisis
Toxinologia
Veneno
Crotalus durissus, Bactericidal activity, Hemolysis, Phospholipases, Rattlesnake, Toxinology, Venom.
Bactericidal activity
Rattlesnake
Crotalus durissus
Phospholipases
Hemolysis
Toxinology
Venom
- Rights
- License
- Derechos de autor 2020 CIENCIA EN DESARROLLO
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| dc.title.en-US.fl_str_mv |
Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima |
| dc.title.es-ES.fl_str_mv |
Actividad Fosfolipasa, Hemolítica y Bactericida preliminar del Veneno de la Serpiente de cascabel del Tolima |
| title |
Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima |
| spellingShingle |
Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima Actividad bactericida Cascabel Crotalus durissus Fosfolipasas Hemolisis Toxinologia Veneno Crotalus durissus, Bactericidal activity, Hemolysis, Phospholipases, Rattlesnake, Toxinology, Venom. Bactericidal activity Rattlesnake Crotalus durissus Phospholipases Hemolysis Toxinology Venom |
| title_short |
Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima |
| title_full |
Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima |
| title_fullStr |
Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima |
| title_full_unstemmed |
Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima |
| title_sort |
Preliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel Tolima |
| dc.subject.es-ES.fl_str_mv |
Actividad bactericida Cascabel Crotalus durissus Fosfolipasas Hemolisis Toxinologia Veneno |
| topic |
Actividad bactericida Cascabel Crotalus durissus Fosfolipasas Hemolisis Toxinologia Veneno Crotalus durissus, Bactericidal activity, Hemolysis, Phospholipases, Rattlesnake, Toxinology, Venom. Bactericidal activity Rattlesnake Crotalus durissus Phospholipases Hemolysis Toxinology Venom |
| dc.subject.en-US.fl_str_mv |
Crotalus durissus, Bactericidal activity, Hemolysis, Phospholipases, Rattlesnake, Toxinology, Venom. Bactericidal activity Rattlesnake Crotalus durissus Phospholipases Hemolysis Toxinology Venom |
| description |
In the department of Tolima, there are no studies that allow us to specify with certainty the magnitude of the ophidic accident caused by Crotalus durissus; Therefore, there is a need to generate toxicological information, as well as knowledge of the potential antimicrobial potential of molecules such as proteins and peptides that make up the venom. In this work, we analyzed the electrophoretic profile by SDS-PAGE of crude venom extracted from individuals collected in the municipality of Natagaima (Tolima) and the association of its components with direct and indirect hemolytic activity on blood agar and its bactericidal activity. The potential of the crude venom against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeuroginosa was evaluated. The venom showed molecular weight bands 26.6 kDa., 17, 14.2, 6.5, 3.5 and 1.06 kDa., corresponding to reports of the venom of this species. The direct and indirect hemolytic activity was dose-dependent, with a minimum hemolytic dose of 200 mg / ml. The antimicrobial effect of the crude venom was differential on the microorganisms evaluated, presenting moderate activity on E. coli. The results constitute valuable data to preliminarily recognize the antimicrobial potential of Crotalus durissus venom (rattle) from the Natagaima-Tolima area. |
| publishDate |
2020 |
| dc.date.accessioned.none.fl_str_mv |
2024-07-08T14:23:58Z |
| dc.date.available.none.fl_str_mv |
2024-07-08T14:23:58Z |
| dc.date.none.fl_str_mv |
2020-01-25 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.identifier.none.fl_str_mv |
https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9869 10.19053/01217488.v11.n1.2020.9869 |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.uptc.edu.co/handle/001/15279 |
| url |
https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9869 https://repositorio.uptc.edu.co/handle/001/15279 |
| identifier_str_mv |
10.19053/01217488.v11.n1.2020.9869 |
| dc.language.none.fl_str_mv |
spa |
| dc.language.iso.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9869/9637 |
| dc.rights.es-ES.fl_str_mv |
Derechos de autor 2020 CIENCIA EN DESARROLLO |
| dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| rights_invalid_str_mv |
Derechos de autor 2020 CIENCIA EN DESARROLLO http://purl.org/coar/access_right/c_abf2 |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.es-ES.fl_str_mv |
Universidad Pedagógica y Tecnológica de Colombia |
| dc.source.en-US.fl_str_mv |
Ciencia En Desarrollo; Vol. 11 No. 1 (2020): Vol 11, Núm.1 (2020): Enero-Junio; 119-127 |
| dc.source.es-ES.fl_str_mv |
Ciencia en Desarrollo; Vol. 11 Núm. 1 (2020): Vol 11, Núm.1 (2020): Enero-Junio; 119-127 |
| dc.source.none.fl_str_mv |
2462-7658 0121-7488 |
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Universidad Pedagógica y Tecnológica de Colombia |
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Repositorio Institucional UPTC |
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repositorio.uptc@uptc.edu.co |
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1839633870010449920 |
| spelling |
2020-01-252024-07-08T14:23:58Z2024-07-08T14:23:58Zhttps://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/986910.19053/01217488.v11.n1.2020.9869https://repositorio.uptc.edu.co/handle/001/15279In the department of Tolima, there are no studies that allow us to specify with certainty the magnitude of the ophidic accident caused by Crotalus durissus; Therefore, there is a need to generate toxicological information, as well as knowledge of the potential antimicrobial potential of molecules such as proteins and peptides that make up the venom. In this work, we analyzed the electrophoretic profile by SDS-PAGE of crude venom extracted from individuals collected in the municipality of Natagaima (Tolima) and the association of its components with direct and indirect hemolytic activity on blood agar and its bactericidal activity. The potential of the crude venom against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeuroginosa was evaluated. The venom showed molecular weight bands 26.6 kDa., 17, 14.2, 6.5, 3.5 and 1.06 kDa., corresponding to reports of the venom of this species. The direct and indirect hemolytic activity was dose-dependent, with a minimum hemolytic dose of 200 mg / ml. The antimicrobial effect of the crude venom was differential on the microorganisms evaluated, presenting moderate activity on E. coli. The results constitute valuable data to preliminarily recognize the antimicrobial potential of Crotalus durissus venom (rattle) from the Natagaima-Tolima area.En el departamento del Tolima no hay estudios que permitan precisar con certeza la magnitud del accidente ofídico causado por Crotalus durissus, existiendo la necesidad de generar información sobre el perfil proteico, como forma de aproximación a la compresión de algunas actividades biológicas relacionadas con la toxicidad del veneno, así como su potencial biotecnológico. En este trabajo se analizó el perfil electroforético por SDS-PAGE del veneno crudo extraído de individuos colectados en el municipio de Natagaima (Tolima) y la asociación con actividades fosfolipasa, hemolítica directa e indirecta y bactericida sobre Escherichia coli, Staphylococcus aureus y Pseudomona aeuroginosa. El veneno crudo presentó bandas de peso molecular 26.6 kDa., 17, 14.2, 6.5, 3.5 y 1.06 kDa., correspondientes con otros reportes previos del veneno para la especie. Se presentaron niveles considerables de actividades hemolítica (200 µg) y fosfolipasa (1.25 UA/mg. ± 0.88) dependientes de Calcio, y el efecto bactericida del veneno crudo fue diferencial sobre los microorganismos evaluados, presentando actividad moderada sobre Escherichia coli. Los resultados constituyen datos valiosos que confieren un acercamiento hacia el conocimiento del potencial tóxico del veneno de Crotalus durissus (cascabel) de la zonade Natagaima-Tolima, así como de la capacidad bactericida y posibles aplicaciones futuras en campos de investigación relacionados con la búsqueda de nuevos agentes antimicrobianos.application/pdfspaspaUniversidad Pedagógica y Tecnológica de Colombiahttps://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/9869/9637Derechos de autor 2020 CIENCIA EN DESARROLLOhttp://purl.org/coar/access_right/c_abf2Ciencia En Desarrollo; Vol. 11 No. 1 (2020): Vol 11, Núm.1 (2020): Enero-Junio; 119-127Ciencia en Desarrollo; Vol. 11 Núm. 1 (2020): Vol 11, Núm.1 (2020): Enero-Junio; 119-1272462-76580121-7488Actividad bactericidaCascabelCrotalus durissusFosfolipasasHemolisisToxinologiaVenenoCrotalus durissus, Bactericidal activity, Hemolysis, Phospholipases, Rattlesnake, Toxinology, Venom.Bactericidal activityRattlesnakeCrotalus durissusPhospholipasesHemolysisToxinologyVenomPreliminary phospholipase, Hemolytic and Bactericidal activity of the venom of the Snake of Cascabel TolimaActividad Fosfolipasa, Hemolítica y Bactericida preliminar del Veneno de la Serpiente de cascabel del Tolimainfo:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1Solano Godoy, Jennifer AlexandraMolano Cardona, Emerson DavidBernal Bautista, Manuel HernandoMurillo Arango, Walter001/15279oai:repositorio.uptc.edu.co:001/152792025-07-18 10:56:40.348metadata.onlyhttps://repositorio.uptc.edu.coRepositorio Institucional UPTCrepositorio.uptc@uptc.edu.co |