Enzymatic quantification method with DNS for ß-mannanase in soybeans and in a nutritional additive

In the search for the decrease in the use of antibiotics in animal feed, the development of nutritional additives that promote animal health and the best use of nutrients in plant-based foods has been reached. In this work, an analytical method was developed to quantify the activity of the enzyme en...

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Autores:
Tipo de recurso:
Fecha de publicación:
2022
Institución:
Universidad Pedagógica y Tecnológica de Colombia
Repositorio:
RiUPTC: Repositorio Institucional UPTC
Idioma:
spa
OAI Identifier:
oai:repositorio.uptc.edu.co:001/10691
Acceso en línea:
https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/14621
https://repositorio.uptc.edu.co/handle/001/10691
Palabra clave:
Enzyme
Sugar
Procedure
Nutrition
Feed
Enzima
Azúcar
Procedimiento
Nutrición
Alimento
Rights
License
Copyright (c) 2022 Danyerly Johana Ruiz Chatez, Natalia Afanasjeva
id REPOUPTC2_bf58dca3e69f3da5b0194de1385416a4
oai_identifier_str oai:repositorio.uptc.edu.co:001/10691
network_acronym_str REPOUPTC2
network_name_str RiUPTC: Repositorio Institucional UPTC
repository_id_str
dc.title.en-US.fl_str_mv Enzymatic quantification method with DNS for ß-mannanase in soybeans and in a nutritional additive
dc.title.es-ES.fl_str_mv Método de cuantificación enzimática con DNS para ß-mananasa en soya y en un aditivo nutricional
title Enzymatic quantification method with DNS for ß-mannanase in soybeans and in a nutritional additive
spellingShingle Enzymatic quantification method with DNS for ß-mannanase in soybeans and in a nutritional additive
Enzyme
Sugar
Procedure
Nutrition
Feed
Enzima
Azúcar
Procedimiento
Nutrición
Alimento
title_short Enzymatic quantification method with DNS for ß-mannanase in soybeans and in a nutritional additive
title_full Enzymatic quantification method with DNS for ß-mannanase in soybeans and in a nutritional additive
title_fullStr Enzymatic quantification method with DNS for ß-mannanase in soybeans and in a nutritional additive
title_full_unstemmed Enzymatic quantification method with DNS for ß-mannanase in soybeans and in a nutritional additive
title_sort Enzymatic quantification method with DNS for ß-mannanase in soybeans and in a nutritional additive
dc.subject.en-US.fl_str_mv Enzyme
Sugar
Procedure
Nutrition
Feed
topic Enzyme
Sugar
Procedure
Nutrition
Feed
Enzima
Azúcar
Procedimiento
Nutrición
Alimento
dc.subject.es-ES.fl_str_mv Enzima
Azúcar
Procedimiento
Nutrición
Alimento
description In the search for the decrease in the use of antibiotics in animal feed, the development of nutritional additives that promote animal health and the best use of nutrients in plant-based foods has been reached. In this work, an analytical method was developed to quantify the activity of the enzyme endo-β-mannanase in a commercial nutritional additive and in soybeans commonly used as a feed source for pigs and chickens. The method used is based on the determination of reducing sugars with 3,5-dinitrosalicylic acid complemented with ultraviolet spectroscopy, where by means of a mannose calibration curve it is possible to determine the amount of sugars released by an equivalent unit of enzyme (U) in the raw material, in a commercial nutritional additive and when added in the soybeans. Additionally, the determination of the enzymatic activity was made by the method of liquid chromatography with an infrared detector, the signal of the mannose monosaccharide standard could be identified, but the separation, identification and subsequent quantification of the reducing sugars produced or released by the enzyme were not achieved enzyme activity in the feed matrix.
publishDate 2022
dc.date.accessioned.none.fl_str_mv 2024-07-05T18:11:42Z
dc.date.available.none.fl_str_mv 2024-07-05T18:11:42Z
dc.date.none.fl_str_mv 2022-12-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
dc.type.en-US.fl_str_mv text
dc.type.es-ES.fl_str_mv texto
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.identifier.none.fl_str_mv https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/14621
10.19053/01228420.v19.n3.2022.14621
dc.identifier.uri.none.fl_str_mv https://repositorio.uptc.edu.co/handle/001/10691
url https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/14621
https://repositorio.uptc.edu.co/handle/001/10691
identifier_str_mv 10.19053/01228420.v19.n3.2022.14621
dc.language.none.fl_str_mv spa
dc.language.iso.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/14621/12419
dc.rights.en-US.fl_str_mv Copyright (c) 2022 Danyerly Johana Ruiz Chatez, Natalia Afanasjeva
http://creativecommons.org/licenses/by/4.0
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Copyright (c) 2022 Danyerly Johana Ruiz Chatez, Natalia Afanasjeva
http://creativecommons.org/licenses/by/4.0
http://purl.org/coar/access_right/c_abf2
dc.format.none.fl_str_mv application/pdf
dc.publisher.en-US.fl_str_mv Universidad Pedagógica y Tecnológica de Colombia
dc.source.en-US.fl_str_mv Ciencia y Agricultura; Vol. 19 No. 3 (2022): Septiembre-Diciembre
dc.source.es-ES.fl_str_mv Ciencia y Agricultura; Vol. 19 Núm. 3 (2022): Septiembre-Diciembre
dc.source.none.fl_str_mv 2539-0899
institution Universidad Pedagógica y Tecnológica de Colombia
repository.name.fl_str_mv Repositorio Institucional UPTC
repository.mail.fl_str_mv repositorio.uptc@uptc.edu.co
_version_ 1839633887233310720
spelling 2022-12-122024-07-05T18:11:42Z2024-07-05T18:11:42Zhttps://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/1462110.19053/01228420.v19.n3.2022.14621https://repositorio.uptc.edu.co/handle/001/10691In the search for the decrease in the use of antibiotics in animal feed, the development of nutritional additives that promote animal health and the best use of nutrients in plant-based foods has been reached. In this work, an analytical method was developed to quantify the activity of the enzyme endo-β-mannanase in a commercial nutritional additive and in soybeans commonly used as a feed source for pigs and chickens. The method used is based on the determination of reducing sugars with 3,5-dinitrosalicylic acid complemented with ultraviolet spectroscopy, where by means of a mannose calibration curve it is possible to determine the amount of sugars released by an equivalent unit of enzyme (U) in the raw material, in a commercial nutritional additive and when added in the soybeans. Additionally, the determination of the enzymatic activity was made by the method of liquid chromatography with an infrared detector, the signal of the mannose monosaccharide standard could be identified, but the separation, identification and subsequent quantification of the reducing sugars produced or released by the enzyme were not achieved enzyme activity in the feed matrix.En la búsqueda de la disminución del uso de antibióticos en la alimentación animal se destaca el desarrollo de aditivos nutricionales que promueven la salud animal y el mejor aprovechamiento de nutrientes en alimentos de origen vegetal. En este trabajo se desarrolló un método analítico para cuantificar la actividad de la enzima endo-β-mananasa en un aditivo nutricional comercial y en la soya comúnmente usada como fuente de alimento para cerdos y pollos. El método usado se basa en la determinación de azúcares reductores con ácido 3,5-dinitrosalicílico complementado con espectroscopia ultravioleta, donde mediante una curva de calibración de manosa se logra determinar la cantidad de azúcares liberados por una unidad equivalente de enzima (U) en la materia prima, en un aditivo nutricional comercial y cuando es adicionado a la soya. Adicionalmente, se hizo la determinación de la actividad enzimática por el método de cromatografía liquida con detector infrarrojo, se pudo identificar la señal del estándar de monosacárido manosa, pero no se logró la separación, identificación y posterior cuantificación de los azucares reductores producidos o liberados por la actividad de la enzima en la matriz del alimento. En este trabajo se desarrolló un método analítico para cuantificar la actividad de la enzima endo-β-mananasa en un aditivo nutricional comercial y en la soya comúnmente usada como fuente de alimento para cerdos y pollos. El método usado se basa en la determinación de azúcares reductores con ácido 3,5-dinitrosalicilico complementado con espectroscopia ultravioleta, donde mediante una curva de calibración de manosa se logra determinar la cantidad de azúcares liberados por una unidad equivalente de enzima (U) en la materia prima, en un aditivo nutricional comercial y cuando es adicionado a la soya. Adicionalmente, se hizo la determinación de la actividad enzimática por el método de cromatografía liquida con detector infrarrojo, se pudo identificar la señal del estándar de monosacárido manosa, pero no se logró la separación, identificación y posterior cuantificación de los azucares reductores producidos o liberados por la actividad de la enzima en la matriz del alimento.application/pdfspaspaUniversidad Pedagógica y Tecnológica de Colombiahttps://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/14621/12419Copyright (c) 2022 Danyerly Johana Ruiz Chatez, Natalia Afanasjevahttp://creativecommons.org/licenses/by/4.0http://purl.org/coar/access_right/c_abf2Ciencia y Agricultura; Vol. 19 No. 3 (2022): Septiembre-DiciembreCiencia y Agricultura; Vol. 19 Núm. 3 (2022): Septiembre-Diciembre2539-0899EnzymeSugarProcedureNutritionFeedEnzimaAzúcarProcedimientoNutriciónAlimentoEnzymatic quantification method with DNS for ß-mannanase in soybeans and in a nutritional additiveMétodo de cuantificación enzimática con DNS para ß-mananasa en soya y en un aditivo nutricionalinfo:eu-repo/semantics/articletexttextohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1Ruiz Chatez, Danyerly JohanaAfanasjeva, Natalia001/10691oai:repositorio.uptc.edu.co:001/106912025-07-18 11:01:29.514metadata.onlyhttps://repositorio.uptc.edu.coRepositorio Institucional UPTCrepositorio.uptc@uptc.edu.co

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