Cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium cepa L.)
(NaClO) sodium hypochlorite is a highly toxic chemical compound widely used because of its disinfectant properties. One of the most commonly used plants to detect different types of genetic changes caused by chemical substances is Allium cepa L., in tests for environmental monitori...
- Autores:
- Tipo de recurso:
- http://purl.org/coar/resource_type/c_7204
- Fecha de publicación:
- 2017
- Institución:
- Universidad Pedagógica y Tecnológica de Colombia
- Repositorio:
- RiUPTC: Repositorio Institucional UPTC
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uptc.edu.co:001/16730
- Acceso en línea:
- https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/5662
https://repositorio.uptc.edu.co/handle/001/16730
- Palabra clave:
- Mitotic index
Metaphase
Anaphase
Anomalies
Índice mitótico
Metafase
Anafase
Anomalías
- Rights
- License
- https://creativecommons.org/licenses/by-nc/4.0/
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| dc.title.en-US.fl_str_mv |
Cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium cepa L.) |
| dc.title.es-ES.fl_str_mv |
Efecto citotóxico del hipoclorito de sodio (NaClO), en células apicales de raíces de cebolla (Allium cepa L.) |
| title |
Cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium cepa L.) |
| spellingShingle |
Cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium cepa L.) Mitotic index Metaphase Anaphase Anomalies Índice mitótico Metafase Anafase Anomalías |
| title_short |
Cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium cepa L.) |
| title_full |
Cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium cepa L.) |
| title_fullStr |
Cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium cepa L.) |
| title_full_unstemmed |
Cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium cepa L.) |
| title_sort |
Cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium cepa L.) |
| dc.subject.en-US.fl_str_mv |
Mitotic index Metaphase Anaphase Anomalies |
| topic |
Mitotic index Metaphase Anaphase Anomalies Índice mitótico Metafase Anafase Anomalías |
| dc.subject.es-ES.fl_str_mv |
Índice mitótico Metafase Anafase Anomalías |
| description |
(NaClO) sodium hypochlorite is a highly toxic chemical compound widely used because of its disinfectant properties. One of the most commonly used plants to detect different types of genetic changes caused by chemical substances is Allium cepa L., in tests for environmental monitoring. The objective of this research was to evaluate the cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium strain L.). Five sodium hypochlorite solutions were prepared at different concentrations, in addition to a control solution, with three replications for each treatment; for 3 days, the growth of the roots was measured; the apices were cut submerging them into hydrochloric acid, subsequently dyed with Aceto-Orcein and were observed under a microscope; the mitotic index (MI) was calculated and the cell anomalies were observed (chromosomal). An analysis of variance (ANOVA) and multiple range tests using the Least Significant Difference method (LSD) were performed. The results showed that there was not a statistically significant difference between the treatments (P |
| publishDate |
2017 |
| dc.date.accessioned.none.fl_str_mv |
2024-07-08T14:42:16Z |
| dc.date.available.none.fl_str_mv |
2024-07-08T14:42:16Z |
| dc.date.none.fl_str_mv |
2017-06-13 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_7204 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/version/c_970fb48d4fbd8a788 |
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http://purl.org/coar/resource_type/c_7204 |
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publishedVersion |
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https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/5662 10.17584/rcch.2017v11i1.5662 |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.uptc.edu.co/handle/001/16730 |
| url |
https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/5662 https://repositorio.uptc.edu.co/handle/001/16730 |
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10.17584/rcch.2017v11i1.5662 |
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spa |
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spa |
| language |
spa |
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https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/5662/pdf |
| dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0/ |
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http://purl.org/coar/access_right/c_abf705 |
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https://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf705 http://purl.org/coar/access_right/c_abf2 |
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application/pdf |
| dc.publisher.en-US.fl_str_mv |
Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
| dc.source.en-US.fl_str_mv |
Revista Colombiana de Ciencias Hortícolas; Vol. 11 No. 1 (2017); 97-104 |
| dc.source.es-ES.fl_str_mv |
Revista Colombiana de Ciencias Hortícolas; Vol. 11 Núm. 1 (2017); 97-104 |
| dc.source.fr-FR.fl_str_mv |
Revista Colombiana de Ciencias Hortícolas; Vol. 11 No 1 (2017); 97-104 |
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Revista Colombiana de Ciencias Hortícolas; V. 11 N. 1 (2017); 97-104 |
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Revista Colombiana de Ciencias Hortícolas; v. 11 n. 1 (2017); 97-104 |
| dc.source.none.fl_str_mv |
2422-3719 2011-2173 |
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Universidad Pedagógica y Tecnológica de Colombia |
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Repositorio Institucional UPTC |
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repositorio.uptc@uptc.edu.co |
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1839633904426811392 |
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2017-06-132024-07-08T14:42:16Z2024-07-08T14:42:16Zhttps://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/566210.17584/rcch.2017v11i1.5662https://repositorio.uptc.edu.co/handle/001/16730(NaClO) sodium hypochlorite is a highly toxic chemical compound widely used because of its disinfectant properties. One of the most commonly used plants to detect different types of genetic changes caused by chemical substances is Allium cepa L., in tests for environmental monitoring. The objective of this research was to evaluate the cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium strain L.). Five sodium hypochlorite solutions were prepared at different concentrations, in addition to a control solution, with three replications for each treatment; for 3 days, the growth of the roots was measured; the apices were cut submerging them into hydrochloric acid, subsequently dyed with Aceto-Orcein and were observed under a microscope; the mitotic index (MI) was calculated and the cell anomalies were observed (chromosomal). An analysis of variance (ANOVA) and multiple range tests using the Least Significant Difference method (LSD) were performed. The results showed that there was not a statistically significant difference between the treatments (PEl hipoclorito de sodio (NaClO), es un compuesto químico altamente tóxico y ampliamente utilizado por sus propiedades desinfectantes. Una de las especies vegetales más usadas para la detección de diferentes tipos de cambios genéticos causados por sustancias químicas es Allium cepa L. mediante la cual se hacen pruebas para la vigilancia del medio ambiente. El objetivo de esta investigación fue evaluar el efecto citotóxico del hipoclorito de sodio (NaClO) en células apicales de raíces de cebolla (Allium cepa L.). Se prepararon cinco soluciones de hipoclorito de sodio a diferentes concentraciones más una solución control con tres réplicas para cada tratamiento; se midió el crecimiento de las raíces durante 3 días, se cortaron los ápices sumergiéndose en ácido clorhídrico, posteriormente fueron teñidos con Aceto-Orceina y se observaron al microscopio; se calculó el índice mitótico (IM) y se observaron las anomalías celulares (cromosómicas). Se realizó un análisis de varianza (ANOVA) y prueba de rangos múltiples mediante el método de diferencia mínima significativa (LSD). Los resultados mostraron que no existe una diferencia estadísticamente significativa entre los tratamientos (P>0,05). La concentración donde se presentó un mayor IM fue de 1 ppm. Se evidenciaron las anomalías celulares: en anafase, evidenciando puentes entre cromosomas, hipercromasia, lesiones nucleares y rompimiento de cromosomas en anafase y metafase. Los resultados evidenciaron que Allium cepa es una especie que ofrece un modelo experimental para evaluar el efecto citotóxico, en este caso del hipoclorito de sodio, el cual generó anomalías celulares (cromosómicas) en todas las concentraciones.application/pdfspaspaSociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTChttps://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/5662/pdfhttps://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf705http://purl.org/coar/access_right/c_abf2Revista Colombiana de Ciencias Hortícolas; Vol. 11 No. 1 (2017); 97-104Revista Colombiana de Ciencias Hortícolas; Vol. 11 Núm. 1 (2017); 97-104Revista Colombiana de Ciencias Hortícolas; Vol. 11 No 1 (2017); 97-104Revista Colombiana de Ciencias Hortícolas; V. 11 N. 1 (2017); 97-104Revista Colombiana de Ciencias Hortícolas; v. 11 n. 1 (2017); 97-1042422-37192011-2173Mitotic indexMetaphaseAnaphaseAnomaliesÍndice mitóticoMetafaseAnafaseAnomalíasCytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium cepa L.)Efecto citotóxico del hipoclorito de sodio (NaClO), en células apicales de raíces de cebolla (Allium cepa L.)info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_7204http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a788http://purl.org/coar/version/c_970fb48d4fbd8a85Causil V., Luis AlfonsoCoronado G., José LuisVerbel M., Luis FernandoVega J., María FernandaDonado E., Keiner AntonioPacheco G., Carluis001/16730oai:repositorio.uptc.edu.co:001/167302025-07-18 11:49:53.582https://creativecommons.org/licenses/by-nc/4.0/metadata.onlyhttps://repositorio.uptc.edu.coRepositorio Institucional UPTCrepositorio.uptc@uptc.edu.co |