Didactic intervention for the extraction and quantification of steroids in urine

This article describes a didactic strategy for teaching a miniaturized solid-phase extractionmethod in the context of laboratory practical training [LPT]. It is based on the students’previous imaginaries in order to generate conceptual changes and promote meaningful learning through a few laboratory sessions. This article will report on the experience of combining the use of a sample preparation technique—PT-SPE—with a didactic intervention exercise for the improvement of procedural skills. We present the results of the application of LPT in the multi-determination of three steroids in urine samples using HPLC-DAD-PT-C18-SPE, which show adequate recovery rates and validation parameters. The results of the intervention show improvement of skills related to solid-phase extraction methods. It is concluded that there were conceptual changes in analytical processes, an adequate familiarization with new sample preparation methodologies, a significant increase in chemical vocabulary,and understanding of concepts, along with the application of lower-order cognitive processes to the enhancement of higher-order cognitive skills.

• Tipo de recurso: http://purl.org/coar/resource_type/c_6509
• Fecha de publicación: 2021
• Institución: Universidad Pedagógica y Tecnológica de Colombia
• Repositorio: RiUPTC: Repositorio Institucional UPTC
• Idioma: spa
• OAI Identifier: oai:repositorio.uptc.edu.co:001/13604
• Acceso en línea: https://revistas.uptc.edu.co/index.php/praxis_saber/article/view/11215; https://repositorio.uptc.edu.co/handle/001/13604
• Palabra clave: experimental science didactics; laboratory practical training; sample preparation; SPE; HPLC; Didáctica de las ciencias experimentales; trabajos prácticos de laboratorio; preparación de muestras; SPE; HPLC; didática das ciências experimentais; prática laboratorial; preparação de amostras; SPE; HPLC
• License: http://creativecommons.org/licenses/by-nc/4.0