Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen

The present investigation focuses on the evaluation of the enzymatic hydrolysis method assisted by ultrasound in obtaining hydrolyzed collagen from the use of the residue (wet white) from leather tanned with glutaraldehyde, in order to minimize the generation of environmental impacts that have occur...

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Tipo de recurso:
Fecha de publicación:
2019
Institución:
Universidad Pedagógica y Tecnológica de Colombia
Repositorio:
RiUPTC: Repositorio Institucional UPTC
Idioma:
spa
OAI Identifier:
oai:repositorio.uptc.edu.co:001/14244
Acceso en línea:
https://revistas.uptc.edu.co/index.php/ingenieria/article/view/9654
https://repositorio.uptc.edu.co/handle/001/14244
Palabra clave:
collagen
environmental impacts
enzymatic hydrolysis
ultrasound
wet white
colágeno
hidrólisis enzimática
impactos ambientales
ultrasonido
wet white
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License
http://purl.org/coar/access_right/c_abf253
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spelling 2019-07-012024-07-05T19:11:50Z2024-07-05T19:11:50Zhttps://revistas.uptc.edu.co/index.php/ingenieria/article/view/965410.19053/01211129.v28.n52.2019.9654https://repositorio.uptc.edu.co/handle/001/14244The present investigation focuses on the evaluation of the enzymatic hydrolysis method assisted by ultrasound in obtaining hydrolyzed collagen from the use of the residue (wet white) from leather tanned with glutaraldehyde, in order to minimize the generation of environmental impacts that have occurred for such material. The effect of pH of the solution, enzyme dose, intensity of applied ultrasound, and temperature of the mixture were evaluated, keeping constant the initial mass of wet white, volume of water and reaction time. The experimental results indicate that for pH 9 doses of enzyme Tan G Plus 0.00012 kg, temperature of 313.15 K and intensity of ultrasound of 40 % it is possible to degrade the material and denature the protein, obtaining a hydrolyzed collagen weight concentration of 3.013 kg / m3, for each kilogram of material used, which represents a 3.0% mass recovery of wet white. It is observed that the most representative variables for the analyzed process are the pH and the temperature, giving a higher performance to the process allowing get a higher amount of hydrolyzed protein.La presente investigación se centra en la evaluación del método de hidrólisis enzimática asistida por ultrasonido en la obtención de colágeno hidrolizado a partir del aprovechamiento del residuo (wet white) proveniente del cuero curtido con glutaraldehído, con la finalidad de minimizar la generación de impactos ambientales por dicho material. Se evaluó el efecto del pH de la solución, dosis de enzima, intensidad de ultrasonido aplicado y temperatura de la mezcla, manteniendo constantes la masa inicial de wet white, el volumen de agua y el tiempo de reacción. Los resultados experimentales señalan que para pH 9 dosis de enzima Tan G Plus 0.00012 kg, temperatura de 313.15 K e intensidad de ultrasonido del 40 % se logra degradar el material y desnaturalizar la proteína, obteniendo una concentración en peso de colágeno hidrolizado de 3.013 kg/m3, por cada kilogramo de material empleado, el cual representa una recuperación del 3.0 % en masa de wet white. Se observa que las variables más representativas para el proceso analizado son el pH y la temperatura, que dan un mayor rendimiento al proceso y permiten obtener mayor cantidad de proteína hidrolizada.application/pdfapplication/xmlspaspaUniversidad Pedagógica y Tecnológica de Colombiahttps://revistas.uptc.edu.co/index.php/ingenieria/article/view/9654/8016https://revistas.uptc.edu.co/index.php/ingenieria/article/view/9654/8249Revista Facultad de Ingeniería; Vol. 28 No. 52 (2019); 59-75Revista Facultad de Ingeniería; Vol. 28 Núm. 52 (2019); 59-752357-53280121-1129collagenenvironmental impactsenzymatic hydrolysisultrasoundwet whitecolágenohidrólisis enzimáticaimpactos ambientalesultrasonidowet whiteEvaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed CollagenEvaluación de la hidrólisis enzimática de wet white asistida con ultrasonido para obtener colágeno hidrolizadoresearchinvestigacióninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a336http://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/access_right/c_abf253http://purl.org/coar/access_right/c_abf2N.A.N.A.Almonacid-Jiménez, Leidy YanethVallejo-Rodríguez, Juan SebastiánAgudelo-Valencia, Rafael NikolayHernández-Fernández, Javier AdolfoOrtiz-Medina, Óscar LeonardoOvalle-González, Diana Paola001/14244oai:repositorio.uptc.edu.co:001/142442025-07-18 11:53:44.199metadata.onlyhttps://repositorio.uptc.edu.coRepositorio Institucional UPTCrepositorio.uptc@uptc.edu.co
dc.title.en-US.fl_str_mv Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen
dc.title.es-ES.fl_str_mv Evaluación de la hidrólisis enzimática de wet white asistida con ultrasonido para obtener colágeno hidrolizado
title Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen
spellingShingle Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen
collagen
environmental impacts
enzymatic hydrolysis
ultrasound
wet white
colágeno
hidrólisis enzimática
impactos ambientales
ultrasonido
wet white
title_short Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen
title_full Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen
title_fullStr Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen
title_full_unstemmed Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen
title_sort Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen
dc.subject.en-US.fl_str_mv collagen
environmental impacts
enzymatic hydrolysis
ultrasound
wet white
topic collagen
environmental impacts
enzymatic hydrolysis
ultrasound
wet white
colágeno
hidrólisis enzimática
impactos ambientales
ultrasonido
wet white
dc.subject.es-ES.fl_str_mv colágeno
hidrólisis enzimática
impactos ambientales
ultrasonido
wet white
description The present investigation focuses on the evaluation of the enzymatic hydrolysis method assisted by ultrasound in obtaining hydrolyzed collagen from the use of the residue (wet white) from leather tanned with glutaraldehyde, in order to minimize the generation of environmental impacts that have occurred for such material. The effect of pH of the solution, enzyme dose, intensity of applied ultrasound, and temperature of the mixture were evaluated, keeping constant the initial mass of wet white, volume of water and reaction time. The experimental results indicate that for pH 9 doses of enzyme Tan G Plus 0.00012 kg, temperature of 313.15 K and intensity of ultrasound of 40 % it is possible to degrade the material and denature the protein, obtaining a hydrolyzed collagen weight concentration of 3.013 kg / m3, for each kilogram of material used, which represents a 3.0% mass recovery of wet white. It is observed that the most representative variables for the analyzed process are the pH and the temperature, giving a higher performance to the process allowing get a higher amount of hydrolyzed protein.
publishDate 2019
dc.date.accessioned.none.fl_str_mv 2024-07-05T19:11:50Z
dc.date.available.none.fl_str_mv 2024-07-05T19:11:50Z
dc.date.none.fl_str_mv 2019-07-01
dc.type.en-US.fl_str_mv research
dc.type.es-ES.fl_str_mv investigación
dc.type.none.fl_str_mv info:eu-repo/semantics/article
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coarversion.spa.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a336
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.uptc.edu.co/index.php/ingenieria/article/view/9654
10.19053/01211129.v28.n52.2019.9654
dc.identifier.uri.none.fl_str_mv https://repositorio.uptc.edu.co/handle/001/14244
url https://revistas.uptc.edu.co/index.php/ingenieria/article/view/9654
https://repositorio.uptc.edu.co/handle/001/14244
identifier_str_mv 10.19053/01211129.v28.n52.2019.9654
dc.language.none.fl_str_mv spa
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistas.uptc.edu.co/index.php/ingenieria/article/view/9654/8016
https://revistas.uptc.edu.co/index.php/ingenieria/article/view/9654/8249
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf253
rights_invalid_str_mv http://purl.org/coar/access_right/c_abf253
http://purl.org/coar/access_right/c_abf2
dc.format.none.fl_str_mv application/pdf
application/xml
dc.coverage.en-US.fl_str_mv N.A.
dc.coverage.es-ES.fl_str_mv N.A.
dc.publisher.en-US.fl_str_mv Universidad Pedagógica y Tecnológica de Colombia
dc.source.en-US.fl_str_mv Revista Facultad de Ingeniería; Vol. 28 No. 52 (2019); 59-75
dc.source.es-ES.fl_str_mv Revista Facultad de Ingeniería; Vol. 28 Núm. 52 (2019); 59-75
dc.source.none.fl_str_mv 2357-5328
0121-1129
institution Universidad Pedagógica y Tecnológica de Colombia
repository.name.fl_str_mv Repositorio Institucional UPTC
repository.mail.fl_str_mv repositorio.uptc@uptc.edu.co
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