Pasantía nacional en diagnóstico molecular de infecciones bacterianas como herramienta estratégica para la certificación sanitaria de centros de producción de trucha arcoíris (Oncorhynchus mykiss) y tilapia (Oreochormis spp) en el departamento de Antioquia
1 recurso en línea (52 páginas) : ilustraciones color, tablas, mapas.
- Autores:
-
Cifuentes Niño, Elkin Darío
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2017
- Institución:
- Universidad Pedagógica y Tecnológica de Colombia
- Repositorio:
- RiUPTC: Repositorio Institucional UPTC
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uptc.edu.co:001/2311
- Acceso en línea:
- http://repositorio.uptc.edu.co/handle/001/2311
- Palabra clave:
- Prácticas agrícolas
Acuicultura - Diagnóstico
Piscicultura - Antioquia (Colombia) - Investigaciones
Trucha arco iris: Oncorhynchus mykiss - Investigaciones
Cría de truchas
Tilapia: Oreochromis mossambicus
Medicina Veterinaria y Zootecnia - Tesis y disertaciones académicas
Agrosavia
- Rights
- openAccess
- License
- Copyright (c) 2017 Universidad Pedagógica y Tecnológica de Colombia
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Pasantía nacional en diagnóstico molecular de infecciones bacterianas como herramienta estratégica para la certificación sanitaria de centros de producción de trucha arcoíris (Oncorhynchus mykiss) y tilapia (Oreochormis spp) en el departamento de Antioquia |
| title |
Pasantía nacional en diagnóstico molecular de infecciones bacterianas como herramienta estratégica para la certificación sanitaria de centros de producción de trucha arcoíris (Oncorhynchus mykiss) y tilapia (Oreochormis spp) en el departamento de Antioquia |
| spellingShingle |
Pasantía nacional en diagnóstico molecular de infecciones bacterianas como herramienta estratégica para la certificación sanitaria de centros de producción de trucha arcoíris (Oncorhynchus mykiss) y tilapia (Oreochormis spp) en el departamento de Antioquia Prácticas agrícolas Acuicultura - Diagnóstico Piscicultura - Antioquia (Colombia) - Investigaciones Trucha arco iris: Oncorhynchus mykiss - Investigaciones Cría de truchas Tilapia: Oreochromis mossambicus Medicina Veterinaria y Zootecnia - Tesis y disertaciones académicas Agrosavia |
| title_short |
Pasantía nacional en diagnóstico molecular de infecciones bacterianas como herramienta estratégica para la certificación sanitaria de centros de producción de trucha arcoíris (Oncorhynchus mykiss) y tilapia (Oreochormis spp) en el departamento de Antioquia |
| title_full |
Pasantía nacional en diagnóstico molecular de infecciones bacterianas como herramienta estratégica para la certificación sanitaria de centros de producción de trucha arcoíris (Oncorhynchus mykiss) y tilapia (Oreochormis spp) en el departamento de Antioquia |
| title_fullStr |
Pasantía nacional en diagnóstico molecular de infecciones bacterianas como herramienta estratégica para la certificación sanitaria de centros de producción de trucha arcoíris (Oncorhynchus mykiss) y tilapia (Oreochormis spp) en el departamento de Antioquia |
| title_full_unstemmed |
Pasantía nacional en diagnóstico molecular de infecciones bacterianas como herramienta estratégica para la certificación sanitaria de centros de producción de trucha arcoíris (Oncorhynchus mykiss) y tilapia (Oreochormis spp) en el departamento de Antioquia |
| title_sort |
Pasantía nacional en diagnóstico molecular de infecciones bacterianas como herramienta estratégica para la certificación sanitaria de centros de producción de trucha arcoíris (Oncorhynchus mykiss) y tilapia (Oreochormis spp) en el departamento de Antioquia |
| dc.creator.fl_str_mv |
Cifuentes Niño, Elkin Darío |
| dc.contributor.advisor.spa.fl_str_mv |
Torres Vidales, Giovanni Cantor Hernández, Cristian Leandro |
| dc.contributor.author.none.fl_str_mv |
Cifuentes Niño, Elkin Darío |
| dc.subject.armarc.none.fl_str_mv |
Prácticas agrícolas Acuicultura - Diagnóstico Piscicultura - Antioquia (Colombia) - Investigaciones Trucha arco iris: Oncorhynchus mykiss - Investigaciones Cría de truchas Tilapia: Oreochromis mossambicus Medicina Veterinaria y Zootecnia - Tesis y disertaciones académicas |
| topic |
Prácticas agrícolas Acuicultura - Diagnóstico Piscicultura - Antioquia (Colombia) - Investigaciones Trucha arco iris: Oncorhynchus mykiss - Investigaciones Cría de truchas Tilapia: Oreochromis mossambicus Medicina Veterinaria y Zootecnia - Tesis y disertaciones académicas Agrosavia |
| dc.subject.armarc.spa.fl_str_mv |
Agrosavia |
| description |
1 recurso en línea (52 páginas) : ilustraciones color, tablas, mapas. |
| publishDate |
2017 |
| dc.date.issued.none.fl_str_mv |
2017 |
| dc.date.accessioned.none.fl_str_mv |
2018-11-28T22:01:59Z |
| dc.date.available.none.fl_str_mv |
2018-11-28T22:01:59Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Pregrado |
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http://purl.org/coar/resource_type/c_7a1f |
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info:eu-repo/semantics/bachelorThesis |
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info:eu-repo/semantics/publishedVersion |
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Text |
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https://purl.org/redcol/resource_type/TP |
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Cifuentes Niño, E. D. (2017). Pasantía nacional en diagnóstico molecular de infecciones bacterianas como herramienta estratégica para la certificación sanitaria de centros de producción de trucha arcoíris (Oncorhynchus mykiss) y tilapia (Oreochormis spp) en el departamento de Antioquia. (Trabajo de pregrado). Universidad Pedagógica y Tecnológica de Colombia, Tunja. http://repositorio.uptc.edu.co/handle/001/2311 |
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http://repositorio.uptc.edu.co/handle/001/2311 |
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Cifuentes Niño, E. D. (2017). Pasantía nacional en diagnóstico molecular de infecciones bacterianas como herramienta estratégica para la certificación sanitaria de centros de producción de trucha arcoíris (Oncorhynchus mykiss) y tilapia (Oreochormis spp) en el departamento de Antioquia. (Trabajo de pregrado). Universidad Pedagógica y Tecnológica de Colombia, Tunja. http://repositorio.uptc.edu.co/handle/001/2311 |
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http://repositorio.uptc.edu.co/handle/001/2311 |
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spa |
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Padrós, F. y M. D. Furones. 2002. Patología bacteriana en piscicul- tura. Temas de Actualidad SEM No 34: 13-21. Holt, R.A., J.S. Rohovec & J.L. Fryer. 1993. Bacterial cold-water disease. In “bacterial disease of fish” (Inglis, V., Roberts, RJ & Bromage, NR. Eds.). Blackwell Scientific - Publica- tions, Oxford, UK. Pp.3-22. Borg, AF. 1960. Studies on myxobacteria associated with diseases in salmonid fishes. American Association for the Advan- cement of Science, Wildlife Disease 8: 1-85, Washington DC, USA. Rangdale, R.E., R.H. Richards & D.J. Aldeman. 1996. Isolation of Cytophaga psychrophila, causal agent of rainbow trout fry syndrome (RTFS) from reproductive fluids and egg surface of rainbow trout (Oncorhynchus mykiss). Bulletin of the European Association Of fish pathologists. 16: 63-67 Dalsgaard, I. 1993. Virulence mechanisms in Cytophaga psychro- phila and other Cytophaga-like bacteria pathogenic for fish. Ann. Rev. Fish Dis. 3: 127-144. Austin, B. & D.A. Austin, 1993. Bacterial fish pathogens: Disease in farmed and wild fish. 2a Edit. Ellis Horwood Ltd., Chi- chester, UK. pp. 228-252. Crump, E.M., M.B. Perry, S.C. Clouthier & W.W. Kay. 2001. Antigenic characterization of the fish pathogen Flavo- bacterium psychrophilum. Appl. Environ. Microbiol. 67 (2): 750-759 Weis, J. 1987. A cold water disease in rainbow trout. Ucber das Vorkommen eimer Kaltwasserkranheir bei Regenbog- enforellen, Salmo gairdneri. Tierdzslicher Umschau. 42: 575-577. Bernardet, J.F., F. Baudin-Laurencin & G. Tixeran. 1988. First identification of Cytophaga psychrophila in France. Bull. Eur. Ass. Fish Pathol. 8: 104-105. Dalsgaard, I. & V. Horlyck. 1990. Cytophaga psychrophila infection in raibow trout in Denmark (abstract). Proc. Conference on Bacterial Disease on Fish. P.37 Santos, Y., P.J.Huntly, A.Turnbull, & T.S. Hastings. 1992. Isolation of Cytophaga psychrophila (Flexibacter psychrophilus) in association with rainbow trout mortality in United King- dom. Bull. Eur. Ass. Fish Pathol. 12:209-210. Toranzo, A.E. & J.L. Barja. 1993. Fry mortality syndrome (FMS) in Spain. Isolation of the causative bacterium Flexibacter psychrophilus. Bull. Eur. Ass. Fish Pathol. 13: 30-32. Wiklund,T., K. Kaas, I. Lonnstron, & I. Dalsgaard. 1994. Isolation of Cytophaga psychrophila (Flexibacter psychrophilus) from wild and farm rainbow trout (Oncorhynchus mykiss) in Finland. Bull. Eurp. Assoc. Fish Path. 14: 44-46. Wakabayashi, H., M. Horiuchi, T. Bunya, & T. Hoshiai. 1991. Outbreaks of cold-water disease in coho salmon in Japan. Fish Pathol. 26: 211-212 Bustos, P.A., J. Calbuyahue, J. Montaña, B. Opazo, P. Entrala & R. Solervicens. 1995. First isolation of Flexibacter psychro- philus, as a causative agent of rainbow trout fry syndrome (RTFS), producing rainbow trout mortality in Chile. Bull. Eur. Ass. Fish Pathol. 15: 162-164. Ekman, E. 2003. Natural and experimental infections with Flavo- bacterium psychropilum in salmonid fish. Doctoral thesis. Swedish University of Agricultural Sciences Uppsala. Cipriano, R.C. & R.A. Holt. 2005. Flavobacterium psychrophilum, cause of Bacterial Cold- Cipriano, R.C. & R.A. Holt. 2005. Flavobacterium psychrophilum, cause of Bacterial Cold-Water Disease and Rainbow Trout Fry Syndrome. Fish Disease Leaflet No. 86. United States Dept. of the Interior. U.S. Geological Service, National Fish Health Research Laboratory, Kearneysville, WV. WOLKE R. E. Pathology of bacterial and fungal diseases affecting fish. In RIBELIN W. E., MIGAKI G.: Pathology of Fishes. University of Wisconsin Press, 1975. Pp. 1004. Robert M. Durborow, Ronald L. Thune, John P. Hawke and A.C. Camus. Columnaris diseases a bacterial infection caused by Flavobacterium columnae. SRAC Publication No. 479, 1998. León J, Ávalos R y Ponce M. Flavobacterium psychrophilum y su patología en alevines de Onchorhynchus mykiss del centro piscícola El Ingenio, Huancayo. Rev. peru. biol. 15(2): 117- 124 (Febrero 2009). Holt, R.A., J.S. Rohovec & J.L. Fryer. 1993. Bacterial cold-water disease. In “bacterial disease of fish” (Inglis, V., Roberts, RJ & Bromage, NR. Eds.). Blackwell Scientific Publica- tions, Oxford, UK. Pp.3-22. Padrós, F. y M. D. Furones. 2002. Patología bacteriana en piscicul- tura. Temas de Actualidad SEM No 34: 13-21. Holt, R.A. 1994. Cold water Disease. Pp. IV: 1-4, in: Suggested procedures for the detection and identification of certain finfish and shellfish pathogens, 4a edition, version 1, Thocsen, JC., ed. Fish Health Section. American Fisheries Society. Bethesda, Maryland. Faruk, M.A., R.E. Campbell, K.D. Thompson, R.E. Rangdale & R.H. Richards. 2002. Characterization of Flavobacterium psychropilum the causative agent of rainbow trout fry syndrome (RTFS) using rabbit antiserum. Bull. Eur. Assoc. Fish Pathol. 22: 354-365. Mata, M., A. Skarmeta & Y. Santos. 2002. A proposed serotyping system for Flavobacterium psychrophilum. Lettr. Appl. Microbiol. 35: 166-170. - Baliarda, D., D. Faure & M.C.Urdaci. 2002. Development and appli- cation of a nested PCR to monitor brood stock salmonid ovarian fluid and spleen for detection of the fish pathogen Flavobacteriun psychrophilum. Journal of Applied Micro- biology 92: 510-516. Ilhan Altinok, Erol Capkin, Sevki Kayis. 2008. Development of multiplex PCR assay for simultaneous detection of five bacterial fish pathogens. Veterinary Microbiology 131, 332–338 lhan Altinok, lknur Kurt. 2003. Molecular Diagnosis of Fish Diseases: a Review. Turkish Journal of Fisheries and Aquatic Sciences 3: 131-138. Holt, R.A., A. Amandi, J.S.Rohovec, & J.L.Fryer. 1989. Relation of water temperature to bacterial cold-water disease in coho salmon, Chinook salmon and rainbow trout. J. Aquat. Animal Health. 1: 94-101. Obach, A., R. Rangdale, & A. Roem. 1995. Rainbow Trout Fry Syn- drome. Aetiology, epidemiology, treatment of Flexibacter psychrophilus infection. ARC UPDATE. 3. Lorenzen, E., I. Dalsgaard, J. From, E.M. Hansen, V. Horlyck, et al. 1991. Preliminary investigations of fry mortality syndrome in rainbow trout. Bull. Eur. Ass. Fish Pathol. 11: 77-79. Bustos, P.A., J. Calbuyahue, J. Montaña, B. Opazo, P. Entrala & R. Solervicens. 1995. First isolation of Flexibacter psychro- philus, as a causative agent of rainbow trout fry syndrome (RTFS), producing rainbow trout mortality in Chile. Bull. Eur. Ass. Fish Pathol. 15: 162-164. Dalsgaard, I. 1993. Virulence mechanisms in Cytophaga psychro- phila and other Cytophaga-like bacteria pathogenic for fish. Ann. Rev. Fish Dis. 3: 127-144. Nematollahi, A., A. Decostere, F. Pasmans, R. Ducatelle, & F. Haesebrouck. 2003. Adhesion of high and low virulent Flavobacterium psychrophilum strains to isolated gill aches of rainbow trout Oncorhynchus mykiss. Diseases of Aquatic Organisms. 55: 101-107 Obach, A. & F. Baudin-Laurencin, 1991. Vaccination of rainbow trout Oncorhynchus mykiss against the visceral form of coldwater disease. Dis. Aquat. Org. 12: 13-15. Bruun, M.S., I.Madsen & I. Dalsgaard. 2003. Efficacy of oxytetra- cycline treatment in rainbow trout experimentally infec- ted with Flavobacterium psychrophilum strains having different in vitro antibiotic susceptibilities. Aquaculture, 215: 11-20. Lorenzen, E. 1994. Studies on Flexibacter psychrophilus in relation to Rainbow Trout Fry Syndrome (RTFS). Ph. D. Thesis. National Veterinary Laboratory, Arhus and Royal Veteri- nary and Agricultural University, Copenhagen Obach, A., R. Rangdale, & A. Roem. 1995. Rainbow Trout Fry Syn- drome. Aetiology, epidemiology, treatment of Flexibacter psychrophilus infection. ARC UPDATE. 3 Kohler, W., 2007. The present state of species within the genera Streptococcus and Enterococcus. International Journal of Medical Microbiology 297 (2007) 133–150. Pier, G.B., Madin, S.H., 1976. Streptococcus iniae sp. nov., a betahemolytic Streptococcus isolated from an Amazon freshwater dolphin, Inia geoffrensis. Int. J. Syst. Bacteriol. 26, 545–553 Facklam, R., Elliott, J., Shewmaker, L., Reingold, A., 2005. Identi- fication and characterization of sporadic isolates of Streptococcus iniae isolated from humans. J. Clin. Microbiol. 43, 933–937 Shoemaker, C.A., Klesius, P.H., Evans, J.J., 2001. Prevalence of Streptococcus iniae in tilapia, hybrid striped bass, and channel catfish on commercial fish farms in the United States. Am. J. Vet. Res. 62, 174–177. Iregui C., Hernández E., Jiménez A., Pulido A., Rey A. L. 2004. Primer mapa epidemiológico de las lesiones y enfermedades de los peces de Colombia. Universidad Nacional de Colombia. Ministerio de Agricultura y Desarrollo Rural .38,39 y 70 pág Weinstein, M.R., M. Litt, D.A. Kertesz, P. Wyper, D. Rose, M. Coulter, A. McGeer, R. Facklam, C. Ostach, B.M. Willey, A. Borczyk, D.E. Low (1997) Invasive infections due to a fish pathogen, Streptococcus iniae. N. Engl. J. Med. 337:589-594 Romano. LA., Mejía J. 2003. Infección por Streptococcus iniae: Una enfermedad emergente que afecta a peces de cultivo y a humanos. Revista AquaTIC, nº 18, pp. 25-32. Bromage, E.S., Owens, L., 2002. Infection of barramundi Lates calcarifer with Streptococcus iniae: effects of different routes of exposure. Dis. Aquat. Org. 52, 199–205. Eldar, A., Bejerano, Y., Livoff, A., Horovitcz, A., Bercovier, H., 1995. Experimental streptococcal meningo-encephalitis in cultured fish. Vet. Microbiol. 43, 33–40. Perera, R., Johnson, S.K., Collins, M.D., Lewis, D.H., 1994. Streptococcus iniae associated with mortality of Tilapia nilotica × T. aurea hybrids. J. Aquat. Anim. Health 6, 335–340 Bercovier, H., Ghittino, C., Eldar, A., 1996. Immunization with bacterial antigens: infections with streptococci and related organisms. In: Second International Symposium on Progress in Fish Vaccinology, Soria Moria Hotel, Oslo, Norway, June 5–7, pp. 153–160. Stoffregen, D., Backman, S., Perham, R., Bowser, P., Babish, J., 1996. Initial disease report of Streptococcus iniae infection in hybrid striped (sunshine) bass and successful therapeutic intervention with the fluoroquinolone antibacterial enrofloxacin. J. World Aquacult. Soc. 27, 420–434. Darwish, A.M., Ismaiel, A.A., 2003. Laboratory efficacy of amoxicillin for the control of Streptococcus iniae infection in sunshine bass. J. Aquat. Anim. Health 15, 209–214. Fuller J.D., D.J. Bast, V. Nizet, D.E. Low, J.C.S. De Azavedo. 2001. Streptococcus iniae Virulence Is Associated with a Distinct Genetic Profile. Infection and Immunity. 69:1994-2000. |
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Copyright (c) 2017 Universidad Pedagógica y Tecnológica de Colombia |
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Torres Vidales, Giovanni561a97b008e38c864ba6e84972e352e6Cantor Hernández, Cristian Leandro7ca37e0ad90957e08e32cf3e6d15ae2dCifuentes Niño, Elkin Darío93822ffb3b79dad40ab7c9249f03b62a2018-11-28T22:01:59Z2018-11-28T22:01:59Z2017Cifuentes Niño, E. D. (2017). Pasantía nacional en diagnóstico molecular de infecciones bacterianas como herramienta estratégica para la certificación sanitaria de centros de producción de trucha arcoíris (Oncorhynchus mykiss) y tilapia (Oreochormis spp) en el departamento de Antioquia. (Trabajo de pregrado). Universidad Pedagógica y Tecnológica de Colombia, Tunja. http://repositorio.uptc.edu.co/handle/001/2311http://repositorio.uptc.edu.co/handle/001/23111 recurso en línea (52 páginas) : ilustraciones color, tablas, mapas.Durante la pasantía se realizó un diagnóstico molecular de infecciones bacterianas como estrategia para la certificación sanitaria de los Centros de producción de trucha arco iris (Oncorhynchus mykiss) y tilapia (Oreochormis spp) en el Departamento de Antioquia con el objetivo de identificar problemáticas en los diferentes eslabones y segmentos de la cadena de producción.Bibliografía: páginas 46-52.El director y codirector aparecen en el trabajo como tutores.PregradoMédico Veterinario Zootecnistaapplication/pdfspaUniversidad Pedagógica y Tecnológica de ColombiaEscuela de Medicina Veterinaria y ZootecniaFacultad de Ciencias AgropecuariasCopyright (c) 2017 Universidad Pedagógica y Tecnológica de Colombiahttps://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2Pasantía nacional en diagnóstico molecular de infecciones bacterianas como herramienta estratégica para la certificación sanitaria de centros de producción de trucha arcoíris (Oncorhynchus mykiss) y tilapia (Oreochormis spp) en el departamento de AntioquiaTrabajo de grado - Pregradohttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/publishedVersionTexthttps://purl.org/redcol/resource_type/TPhttp://purl.org/coar/version/c_970fb48d4fbd8a85Padrós, F. y M. D. Furones. 2002. Patología bacteriana en piscicul- tura. Temas de Actualidad SEM No 34: 13-21.Holt, R.A., J.S. Rohovec & J.L. Fryer. 1993. Bacterial cold-water disease. In “bacterial disease of fish” (Inglis, V., Roberts, RJ & Bromage, NR. Eds.). Blackwell Scientific - Publica- tions, Oxford, UK. Pp.3-22.Borg, AF. 1960. Studies on myxobacteria associated with diseases in salmonid fishes. American Association for the Advan- cement of Science, Wildlife Disease 8: 1-85, Washington DC, USA.Rangdale, R.E., R.H. Richards & D.J. Aldeman. 1996. Isolation of Cytophaga psychrophila, causal agent of rainbow trout fry syndrome (RTFS) from reproductive fluids and egg surface of rainbow trout (Oncorhynchus mykiss). Bulletin of the European Association Of fish pathologists. 16: 63-67Dalsgaard, I. 1993. Virulence mechanisms in Cytophaga psychro- phila and other Cytophaga-like bacteria pathogenic for fish. Ann. Rev. Fish Dis. 3: 127-144.Austin, B. & D.A. Austin, 1993. Bacterial fish pathogens: Disease in farmed and wild fish. 2a Edit. Ellis Horwood Ltd., Chi- chester, UK. pp. 228-252.Crump, E.M., M.B. Perry, S.C. Clouthier & W.W. Kay. 2001. Antigenic characterization of the fish pathogen Flavo- bacterium psychrophilum. Appl. Environ. Microbiol. 67 (2): 750-759Weis, J. 1987. A cold water disease in rainbow trout. Ucber das Vorkommen eimerKaltwasserkranheir bei Regenbog- enforellen, Salmo gairdneri. Tierdzslicher Umschau. 42: 575-577.Bernardet, J.F., F. Baudin-Laurencin & G. Tixeran. 1988. First identification of Cytophaga psychrophila in France. Bull. Eur. Ass. Fish Pathol. 8: 104-105.Dalsgaard, I. & V. Horlyck. 1990. Cytophaga psychrophila infection in raibow trout in Denmark (abstract). Proc. Conference on Bacterial Disease on Fish. P.37Santos, Y., P.J.Huntly, A.Turnbull, & T.S. Hastings. 1992. Isolation of Cytophaga psychrophila (Flexibacter psychrophilus) in association with rainbow trout mortality in United King- dom. Bull. Eur. Ass. Fish Pathol. 12:209-210.Toranzo, A.E. & J.L. Barja. 1993. Fry mortality syndrome (FMS) in Spain. Isolation of the causative bacterium Flexibacter psychrophilus. Bull. Eur. Ass. Fish Pathol. 13: 30-32.Wiklund,T., K. Kaas, I. Lonnstron, & I. Dalsgaard. 1994. Isolation of Cytophaga psychrophila (Flexibacter psychrophilus) from wild and farm rainbow trout (Oncorhynchus mykiss) in Finland. Bull. Eurp. Assoc. Fish Path. 14: 44-46.Wakabayashi, H., M. Horiuchi, T. Bunya, & T. Hoshiai. 1991. Outbreaks of cold-water disease in coho salmon in Japan. Fish Pathol. 26: 211-212Bustos, P.A., J. Calbuyahue, J. Montaña, B. Opazo, P. Entrala & R. Solervicens. 1995. First isolation of Flexibacter psychro- philus, as a causative agent of rainbow trout fry syndrome (RTFS), producing rainbow trout mortality in Chile. Bull. Eur. Ass. Fish Pathol. 15: 162-164.Ekman, E. 2003. Natural and experimental infections with Flavo- bacterium psychropilum in salmonid fish. Doctoral thesis. Swedish University of Agricultural Sciences Uppsala.Cipriano, R.C. & R.A. Holt. 2005. Flavobacterium psychrophilum, cause of Bacterial Cold-Cipriano, R.C. & R.A. Holt. 2005. Flavobacterium psychrophilum, cause of Bacterial Cold-Water Disease and Rainbow Trout Fry Syndrome. Fish Disease Leaflet No. 86. United States Dept. of the Interior. U.S. Geological Service, National Fish Health Research Laboratory, Kearneysville, WV.WOLKE R. E. Pathology of bacterial and fungal diseases affecting fish. In RIBELIN W. E., MIGAKI G.: Pathology of Fishes. University of Wisconsin Press, 1975. Pp. 1004.Robert M. Durborow, Ronald L. Thune, John P. Hawke and A.C. Camus. Columnaris diseases a bacterial infection caused by Flavobacterium columnae. SRAC Publication No. 479, 1998.León J, Ávalos R y Ponce M. Flavobacterium psychrophilum y su patología en alevines de Onchorhynchus mykiss del centro piscícola El Ingenio, Huancayo. Rev. peru. biol. 15(2): 117- 124 (Febrero 2009).Holt, R.A., J.S. Rohovec & J.L. Fryer. 1993. Bacterial cold-water disease. In “bacterial disease of fish” (Inglis, V., Roberts, RJ & Bromage, NR. Eds.). Blackwell Scientific Publica- tions, Oxford, UK. Pp.3-22.Padrós, F. y M. D. Furones. 2002. Patología bacteriana en piscicul- tura. Temas de Actualidad SEM No 34: 13-21.Holt, R.A. 1994. Cold water Disease. Pp. IV: 1-4, in: Suggested procedures for the detection and identification of certain finfish and shellfish pathogens, 4a edition, version 1, Thocsen, JC., ed. Fish Health Section. American Fisheries Society. Bethesda, Maryland.Faruk, M.A., R.E. Campbell, K.D. Thompson, R.E. Rangdale & R.H. Richards. 2002. Characterization of Flavobacterium psychropilum the causative agent of rainbow trout fry syndrome (RTFS) using rabbit antiserum. Bull. Eur. Assoc. Fish Pathol. 22: 354-365.Mata, M., A. Skarmeta & Y. Santos. 2002. A proposed serotyping system for Flavobacterium psychrophilum. Lettr. Appl. Microbiol. 35: 166-170. -Baliarda, D., D. Faure & M.C.Urdaci. 2002. Development and appli- cation of a nested PCR to monitor brood stock salmonid ovarian fluid and spleen for detection of the fish pathogen Flavobacteriun psychrophilum. Journal of Applied Micro- biology 92: 510-516.Ilhan Altinok, Erol Capkin, Sevki Kayis. 2008. Development of multiplex PCR assay for simultaneous detection of five bacterial fish pathogens. Veterinary Microbiology 131, 332–338lhan Altinok, lknur Kurt. 2003. Molecular Diagnosis of Fish Diseases: a Review. Turkish Journal of Fisheries and Aquatic Sciences 3: 131-138.Holt, R.A., A. Amandi, J.S.Rohovec, & J.L.Fryer. 1989. Relation of water temperature to bacterial cold-water disease in coho salmon, Chinook salmon and rainbow trout. J. Aquat. Animal Health. 1: 94-101.Obach, A., R. Rangdale, & A. Roem. 1995. Rainbow Trout Fry Syn- drome. Aetiology, epidemiology, treatment of Flexibacter psychrophilus infection. ARC UPDATE. 3.Lorenzen, E., I. Dalsgaard, J. From, E.M. Hansen, V. Horlyck, et al. 1991. Preliminary investigations of fry mortality syndrome in rainbow trout. Bull. Eur. Ass. Fish Pathol. 11: 77-79.Bustos, P.A., J. Calbuyahue, J. Montaña, B. Opazo, P. Entrala & R. Solervicens. 1995. First isolation of Flexibacter psychro- philus, as a causative agent of rainbow trout fry syndrome (RTFS), producing rainbow trout mortality in Chile. Bull. Eur. Ass. Fish Pathol. 15: 162-164.Dalsgaard, I. 1993. Virulence mechanisms in Cytophaga psychro- phila and other Cytophaga-like bacteria pathogenic for fish. Ann. Rev. Fish Dis. 3: 127-144.Nematollahi, A., A. Decostere, F. Pasmans, R. Ducatelle, & F. Haesebrouck. 2003. Adhesion of high and low virulent Flavobacterium psychrophilum strains to isolated gill aches of rainbow trout Oncorhynchus mykiss. Diseases of Aquatic Organisms. 55: 101-107Obach, A. & F. Baudin-Laurencin, 1991. Vaccination of rainbow trout Oncorhynchus mykiss against the visceral form of coldwater disease. Dis. Aquat. Org. 12: 13-15.Bruun, M.S., I.Madsen & I. Dalsgaard. 2003. Efficacy of oxytetra- cycline treatment in rainbow trout experimentally infec- ted with Flavobacterium psychrophilum strains having different in vitro antibiotic susceptibilities. Aquaculture, 215: 11-20.Lorenzen, E. 1994. Studies on Flexibacter psychrophilus in relation to Rainbow Trout Fry Syndrome (RTFS). Ph. D. Thesis. National Veterinary Laboratory, Arhus and Royal Veteri- nary and Agricultural University, CopenhagenObach, A., R. Rangdale, & A. Roem. 1995. Rainbow Trout Fry Syn- drome. Aetiology, epidemiology, treatment of Flexibacter psychrophilus infection. ARC UPDATE. 3Kohler, W., 2007. The present state of species within the genera Streptococcus and Enterococcus. International Journal of Medical Microbiology 297 (2007) 133–150.Pier, G.B., Madin, S.H., 1976. Streptococcus iniae sp. nov., a betahemolytic Streptococcus isolated from an Amazon freshwater dolphin, Inia geoffrensis. Int. J. Syst. Bacteriol. 26, 545–553Facklam, R., Elliott, J., Shewmaker, L., Reingold, A., 2005. Identi- fication and characterization of sporadic isolates of Streptococcus iniae isolated from humans. J. Clin. Microbiol. 43, 933–937Shoemaker, C.A., Klesius, P.H., Evans, J.J., 2001. Prevalence of Streptococcus iniae in tilapia, hybrid striped bass, and channel catfish on commercial fish farms in the United States. Am. J. Vet. Res. 62, 174–177.Iregui C., Hernández E., Jiménez A., Pulido A., Rey A. L. 2004. Primer mapa epidemiológico de las lesiones y enfermedades de los peces de Colombia. Universidad Nacional de Colombia. Ministerio de Agricultura y Desarrollo Rural .38,39 y 70 págWeinstein, M.R., M. Litt, D.A. Kertesz, P. Wyper, D. Rose, M. Coulter, A. McGeer, R. Facklam, C. Ostach, B.M. Willey, A. Borczyk, D.E. Low (1997) Invasive infections due to a fish pathogen, Streptococcus iniae. N. Engl. J. Med. 337:589-594Romano. LA., Mejía J. 2003. Infección por Streptococcus iniae: Una enfermedad emergente que afecta a peces de cultivo y a humanos. Revista AquaTIC, nº 18, pp. 25-32.Bromage, E.S., Owens, L., 2002. Infection of barramundi Lates calcarifer with Streptococcus iniae: effects of different routes of exposure. Dis. Aquat. Org. 52, 199–205.Eldar, A., Bejerano, Y., Livoff, A., Horovitcz, A., Bercovier, H., 1995. Experimental streptococcal meningo-encephalitis in cultured fish. Vet. Microbiol. 43, 33–40.Perera, R., Johnson, S.K., Collins, M.D., Lewis, D.H., 1994. Streptococcus iniae associated with mortality of Tilapia nilotica × T. aurea hybrids. J. Aquat. Anim. Health 6, 335–340Bercovier, H., Ghittino, C., Eldar, A., 1996. Immunization with bacterial antigens: infections with streptococci and related organisms. In: Second International Symposium on Progress in Fish Vaccinology, Soria Moria Hotel, Oslo, Norway, June 5–7, pp. 153–160.Stoffregen, D., Backman, S., Perham, R., Bowser, P., Babish, J., 1996. Initial disease report of Streptococcus iniae infection in hybrid striped (sunshine) bass and successful therapeutic intervention with the fluoroquinolone antibacterial enrofloxacin. J. World Aquacult. Soc. 27, 420–434.Darwish, A.M., Ismaiel, A.A., 2003. Laboratory efficacy of amoxicillin for the control of Streptococcus iniae infection in sunshine bass. J. Aquat. Anim. Health 15, 209–214.Fuller J.D., D.J. Bast, V. Nizet, D.E. Low, J.C.S. De Azavedo. 2001. Streptococcus iniae Virulence Is Associated with a Distinct Genetic Profile. Infection and Immunity. 69:1994-2000.Prácticas agrícolasAcuicultura - DiagnósticoPiscicultura - Antioquia (Colombia) - InvestigacionesTrucha arco iris: Oncorhynchus mykiss - InvestigacionesCría de truchasTilapia: Oreochromis mossambicusMedicina Veterinaria y Zootecnia - Tesis y disertaciones académicasAgrosaviaORIGINALTGT-946.pdfTGT-946.pdfArchivo principalapplication/pdf2267945https://repositorio.uptc.edu.co/bitstreams/ee04eac7-1d29-42fc-a5e4-1642629584c7/download81e9baa040a9e424a8aad0ec7b3fa9b5MD51A_EDCN.pdfA_EDCN.pdfAutorización publicaciónapplication/pdf574908https://repositorio.uptc.edu.co/bitstreams/7ab10f38-a4bb-48ae-a7b5-a7e54eac15b7/download5366ee371cc4cfa2a5b5c27c8b2a6caeMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-814798https://repositorio.uptc.edu.co/bitstreams/246df0a1-8990-43d5-875a-a6a01d8b6945/download88794144ff048353b359a3174871b0d5MD53TEXTTGT-946.pdf.txtTGT-946.pdf.txtExtracted 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