Pasantía en el Laboratorio de Biotecnología Animal de la Facultad De Ciencias Agrarias del Politécnico Colombiano Jaime Isaza Cadavid
1 recurso en línea (79 páginas) : ilustraciones color, tablas, figuras.
- Autores:
-
Maldonado Castro, Gerardo Antonio
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2018
- Institución:
- Universidad Pedagógica y Tecnológica de Colombia
- Repositorio:
- RiUPTC: Repositorio Institucional UPTC
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uptc.edu.co:001/2336
- Acceso en línea:
- http://repositorio.uptc.edu.co/handle/001/2336
- Palabra clave:
- Mejoramiento genético
Obstetricia veterinaria
Reproducción animal
Embriología
Transplante de embriones
Inseminación artificial
Inseminación
Medicina Veterinaria y Zootecnia - Tesis y disertaciones académicas
Agrosavia
- Rights
- openAccess
- License
- Copyright (c) 2018 Universidad Pedagógica y Tecnológica de Colombia
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Pasantía en el Laboratorio de Biotecnología Animal de la Facultad De Ciencias Agrarias del Politécnico Colombiano Jaime Isaza Cadavid |
| title |
Pasantía en el Laboratorio de Biotecnología Animal de la Facultad De Ciencias Agrarias del Politécnico Colombiano Jaime Isaza Cadavid |
| spellingShingle |
Pasantía en el Laboratorio de Biotecnología Animal de la Facultad De Ciencias Agrarias del Politécnico Colombiano Jaime Isaza Cadavid Mejoramiento genético Obstetricia veterinaria Reproducción animal Embriología Transplante de embriones Inseminación artificial Inseminación Medicina Veterinaria y Zootecnia - Tesis y disertaciones académicas Agrosavia |
| title_short |
Pasantía en el Laboratorio de Biotecnología Animal de la Facultad De Ciencias Agrarias del Politécnico Colombiano Jaime Isaza Cadavid |
| title_full |
Pasantía en el Laboratorio de Biotecnología Animal de la Facultad De Ciencias Agrarias del Politécnico Colombiano Jaime Isaza Cadavid |
| title_fullStr |
Pasantía en el Laboratorio de Biotecnología Animal de la Facultad De Ciencias Agrarias del Politécnico Colombiano Jaime Isaza Cadavid |
| title_full_unstemmed |
Pasantía en el Laboratorio de Biotecnología Animal de la Facultad De Ciencias Agrarias del Politécnico Colombiano Jaime Isaza Cadavid |
| title_sort |
Pasantía en el Laboratorio de Biotecnología Animal de la Facultad De Ciencias Agrarias del Politécnico Colombiano Jaime Isaza Cadavid |
| dc.creator.fl_str_mv |
Maldonado Castro, Gerardo Antonio |
| dc.contributor.advisor.spa.fl_str_mv |
Porras Vargas, José Luis Gómez Oquendo, Jorge |
| dc.contributor.author.none.fl_str_mv |
Maldonado Castro, Gerardo Antonio |
| dc.subject.armarc.none.fl_str_mv |
Mejoramiento genético Obstetricia veterinaria Reproducción animal Embriología Transplante de embriones Inseminación artificial Inseminación Medicina Veterinaria y Zootecnia - Tesis y disertaciones académicas |
| topic |
Mejoramiento genético Obstetricia veterinaria Reproducción animal Embriología Transplante de embriones Inseminación artificial Inseminación Medicina Veterinaria y Zootecnia - Tesis y disertaciones académicas Agrosavia |
| dc.subject.armarc.spa.fl_str_mv |
Agrosavia |
| description |
1 recurso en línea (79 páginas) : ilustraciones color, tablas, figuras. |
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2018 |
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2018-12-12T14:29:57Z |
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2018-12-12T14:29:57Z |
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2018 |
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Trabajo de grado - Pregrado |
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Maldonado Castro, G. A. (2018). Pasantía en el Laboratorio de Biotecnología Animal de la Facultad De Ciencias Agrarias del Politécnico Colombiano Jaime Isaza Cadavid. (Trabajo de pregrado). Universidad Pedagógica y Tecnológica de Colombia, Tunja. http://repositorio.uptc.edu.co/handle/001/2336 |
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Maldonado Castro, G. A. (2018). Pasantía en el Laboratorio de Biotecnología Animal de la Facultad De Ciencias Agrarias del Politécnico Colombiano Jaime Isaza Cadavid. (Trabajo de pregrado). Universidad Pedagógica y Tecnológica de Colombia, Tunja. http://repositorio.uptc.edu.co/handle/001/2336 |
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spa |
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Avery, B., Greve, T. 1995. Impact of percollâ on bovine spermatozoa usedfor in vitro insemination. Theriogenology. Vol. 44, pp.871-878. Ball GD, Coulan CB, FieldCS, Harms RW, Thie JT, Byers AP. 1985. Effects of serumsource on human fertilization and embryonic growth parameters in vitro. Fertil Steril. 44:75-79. Bavister BD. 1992. Co-culture forembryo development: is it really necessary? Human Reproduction: 10:1339-1341. Brackett, B. D., Oliphant, G. 1975. Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. Vol. 12, pp. 260-274. Báez Contreras, Francisco J, Chávez Corona, Adeymi C, Hernández Fonseca, Hugo J, & Villamediana Monreal, Patricia C. 2010. Evaluation of in vitro development capacity of bovine oocytes obtained from predominantly Bos taurus and Bos indicus cows. Revista Científica, 20(3), 259-267. Recuperado, febrero de 2018, de http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0798- 22592010000300007&lng=es&tlng=en Cabodevila J., Teruel M,. 2001. Criopreservación de embriones bovinos. En: Biotecnología de la reproducción. (ed.) Instituto Nacional de Tecnología Agropecuaria INTA. Argentina, pp. 149-174 Cabrera P., Fernández A. 2006. Criopreservación de Embriones: una herramienta básica en la Reproducción Asistida. Revista de la Facultad de Ciencias Veterinaria Central de Venezuela 2009. Venezuela. Publicado en Crio protectores. Publicado en internet, disponible en Revista de la Facultad de Ciencias Veterinarias. Universidad Central de Venezuela. Catena, M; Cabodevila, J. 1999. Evaluación del semen bovino.Simposio Internacional de Reproduccion Bovina (UNCPBA).Tandil. Taurus. 1(3):18-31. Recuperado, febrero de 2018, de http://www2.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0258- 65762006000200001&lng=es&nrm=iso Activo agosto 2009 Colenbrander, B.; Gadella, B.; Stout, T. 2003. The predictive value of semen analysis in the evaluation of the stallion fertility. Reprod. Dom. Anim. 38(4):305- 311. Crespo J.L y Guamán E. G. 2015. Fertilización in vitro en bovinos en el Laboratorio de Reproducción Animal de Zamorano utilizando el protocolo de Genes Difusión. Tesis de pregrado en Ingeniería Agronómica. Escuela Agrícola Panamericana, Zamorano Honduras. Cruz P, Medina V, Robles, Velasco Y. 2006 Evaluación de diferentes crioprotectores para la crioconservación de espermatozoides de yamú (Bryconamazonicus). Revista Colombiana de Ciencias Pecuarias. 2006; 19(2):153-159. Eagle H. 1959. Amino acid metabolis minmammalian cells. Science 130:432- 437. FAO. 2012. Ganadería Mundial 2011. La ganadería en la seguridad alimentaria (en línea). Roma, FAO. Recuperado, febrero de 2018; Disponible en: http://www.fao.org/docrep/016/i2373s/i2373s00.pdf Fernández, Adriana, Díaz, Thaís, & Muñoz, Gladys. 2007. Producción In vitro de Embriones Bovinos. Revista de la Facultad de Ciencias Veterinarias, 48(1), 51-60. Recuperado en 03 de febrero de 2018, de http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0258- 65762007000100006&lng=es&tlng=es. Fry, R. C., Niall, E. M., Simpson, T. L., Squires, T. J., Reynolds, J. 1997. The collection of oocytes from bovine ovaries. Theriogenology. Vol. 47, pp. 977-987. Fukuda, Y., Ichikawa, M., Naito, K., Toyoda, Y. 1990. Birth of normal calves resulting from bovine oocytes matured, fertilized, and cultured with cumulus cells in vitro up to blastocyst stage. Biol. Reprod. Vol. 42, pp. 114-119. GandolfiF, BreviniTA, MoorRM. 1989. Effect of oviduct environment on embryonic development. J ReprodFert. 38:107-115. Gardner, HG, Kaye PL. 1991. Insulinincreases cell numbers andmorphological development inmouse pre-implantationembryos in vitro. ReprodFert Dev 3:79- 91. Geshi, M., Takenouchi, N., Yameuchi,N., Nagai,T. 2000. Effect of sodium piruvate in nonserum maturation medium o maduration, fertilization, and subsequent development of bovine oocytes with or without cumulus cells. Biology of reproduction,63: 1730-1734. Gilardi SGT, Pinho TG, Martins CB. Perímetro escrotal características do sêmen de touros Nelore aos 18 meses de idade. Rev Bras Cienc Vet. 2001;8:13-15. Gordon, I., y K.H. Lu. 1990. Production of embryos in vitro and it's impact on livestock production. Theriogenology 33:77-87. Graham, J.; Mocé, E. 2005. Fertility evaluation of frozen/thawed semen. Theriogenology. 64:492-504. Gutierrez Adan A, Granados J, Pintado B y De laFuente J. 2001. Influence of the glucose onthe sex ratio of bovine IVM-IVFembryos culturedin vitro. Reprod. Fértil. & Develop. 13, 361-365. Hazeleger, N.L., D. J. Hill, R.B. Stubbings y J.S. Walton. 1995. Relationship of morphology and follicular fluid environment of oocytes to their developmental potential in vitro.Theriogenology, 43:502-509. Hidalgo, C; Tamargo, C; Diez, C. 2005. Análisis del semen bovino. Asturias, España. Información ganadera. 39 Boletín Informativo SERIDA. No. 2. p. 39-43 Hofig, A, Simmen FA, Simmen RCM. 1990. Effects of dihydroepiandrosterone (DHEA), insulin-like growthfactor(IGF-1), Insulin(I) onestradiol productionby porcine conceptuses in vitro. Biol Reprod. 42 (Suppl. 1), 135. Hyttel, P., Xu, K. P., Smith, S., Greve, T. 1986. Ultrastructure of in vitro oocyte maturation in cattle.J. Reprod. Fert. Vol. 78, pp. 615-625. Recuperado, febrero de 2018, de http://www.monografias.com/trabajos903/vitrificacion tecnicacrioconservacion/vitrificacion-tecnica-crioconservacion2.shtml Kastelic JP, Cook RB, Pierson RA, Coulter GH. Relationships among scrotal and testicular characteristics, sperm production, and seminal quality in 129 beef bulls. Can J Vet Res. 2001;65(2):111-5. Kane MT. 1988. The effects of water-soluble vitamins on the expansion of rabbit blastocysts in vitro. J Exp Zool. 245:220-223. Keskintepe L, Clay A, Burnley y Brackett BG. 1995. Production of viable bovine blastocysts indefined in vitro conditions. Biol Reprod. 52:1410-1417. Kim JH, Funahashi H, Niwa K, , Okuda K. 1993. Glucose requirement at different developmental stages of in vitro fertilized bovine embryos cultured in semi-definedmedium. Theriogenology 37:875-886. KobayashiK, SatohT, Yamashita, S, Hoshi, H. 1994. Low oxygen level and glucose improves the development of fertilized bovine oocytes in defined medium without somatic cells. In vitro cell Dev Biol. 30A:556-558. Kruip Tham, boni R, Wurth YA, Roelofsen MWM, mc Pieterse 1994 potential use of ovum pick-up for embryoproduction and breeding in cattle therionology 42,675-684. Kuwayama, M.; Hamano, S.; Nagai, T. 1992. Vitrification of bovine blastocysts obtained by in vitro culture of oocytes matured and fertilized in vitro. J. Reprod. Fert. 96:187-193 Lane M, Gardner DK. 1997. Mouse embryo development is differentially regulated by amino acids. J ReprodFertil 109:153-164. Leese HJ. 1988. The formation and function of oviduct fluid. J ReprodFert. 82:843-856. Leifried, L.,First, N 1979. Characterization of bovine follicular oocytes and their ability to mature in vitro.J Anim. Sc 48:76-83. Leibfried-Rutledge, M. L., Critser, E. S., First, N. L. 1986. Effects of fetal calf serum and bovine serum albumin on in vitro maturation and fertilization of bovine and hamster cumulus-oocyte complexes. Biol. Reprod. 35:850-857 Lehninger, AL. 1975. Biochemistry. WorthPublishers, NewYork. Leoni, G.; Bogliolo, L.; Berlinguer, E; Rosati, L; Pintus, P P; Ledda, S.; Naitana, S. 2002. Defined media for vitrification, warming, and rehydration: effects on post-thaw protein synthesis and viability of in vitro derived ovine embryos. Cryobiology 45:204-2012. Lessley, B. A., Garner, D. L. 1983. Isolation of motile spermatozoa by density radient centrifugation in percoll. Gamate Res. Vol. 7, pp. 49-65. Lonergan, P.,Rizos,D., Kanka,L., Nemcova,L., Mbaye,AM., Kingston,M., Wade,M., Duffy,P., Boland,M. 2003. Temporal sensitivity of bovine embryos to culture environment after fertilization and the implications for blastocyst quality. Reproduction 126,337–346. Lonergan,P.,Vergos, E., Kinis,A.,Sharif,H., Gordon,I. 1991. The effect of recovery method o the type of bovine oocytes obtained for IVM.Theriogenology,35:231. Lonergan, P.,Rizos,D., Kanka,L., Nemcova,L., Mbaye,AM., Kingston,M., Wade,M., Duffy,P., Boland,M. 2003. Temporal sensitivity of bovine embryos to culture environment after fertilization and the implications for blastocyst quality. Reproduction Lu, K. H., Gordon, I., Gallager, M., Mcgovern, H. 1987. Pregnancy established in cattle by transfer of embryos derived from in vitro fertilization of oocytes matured in vitro. Vet. Rec. Vol. 121, pp. 259-260. Madan, M.L. 2005. Animal biotechnology: applications and economic implication in developing countries. Revue Scientifique et Technique Office International Des Epizooties 24(1): 123-139. Marguant-Le Guienne B, Gerard M, Solari A y Thibault C. 1989. In vitro culture of bovine eggs fertilized eitherinvivo or in vitro. ReprodNutrDev 29:559-568. Massip, A.; Van Der Zwalmen, P; Ecotrs, E. 1987. Recent progress in cryopreservation of cattle embryos. Theriogenology 27:69-79 Morillo, M; Salazar, S; Castillo, E. 2012. Evaluación del potencial reproductivo del macho bovino. Maracay, VE, Instituto Nacional de Investigaciones Agrícolas. 60 p. Centro Nacional de Investigaciones Agropecuarias Niwa, K., Ohoda, O. 1988. Synergistic effect of caffeine and heparin on in-vitro fertilization of cattle oocytes matured in culture. Theriogenology. Vol. 30, pp. 733-741. Oyuela L.A. y Jimenez C, 2010. Factores que afectan la tasa de preñez en programas de transferencia de embriones. Med. Vet. Zoot. 2010. 57:191-200 Página Web del Politécnico Colombiano JAIME ISAZA CADAVID. Recuperado, febrero de 2018, de http://www.politecnicojic.edu.co/index.php/laboratoriosagrarias. Palasz AT, Tornesi MB, Archer J y Mapletoft RJ. 1995. Media alternatives for the collection, culture and freezing of mouse and cattle embryos. Theriogenology 44:705-714. Parrish, J. J., Susko-Parrish, J., Leibfried-Rutledge, M. L., Crister, E. S., Eyestone, W. H., First, N. L. 1986. Bovine in vitro fertilization with frozenthawed semen. Theriogenology. Vol. 25, pp. 591-600. Peláez, V.A 2011. Producción in vitro de embriones bovinos. Monografía Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Escuela de Medicina Veterinaria y Zootecnia. Ecuador. Ral1, W E; Fahy, G. M. 1985. Ice-free cryopreservation of mouse embryo at - 196°- C by vitrification. Nature 313:573- 575 Reyes, S. 1995. Fecundación in vitro: Una nueva reproducción. Revista de Extensión TecnoVet. 2 (1): 1-3. Riddel KP, Stringfellow DA Gray BW, Riddel, JrMG. 1985. Effects of antibiotics ondevelopment capacity of bovine embryos. Bov Pract. 30:94. Rieger D, Loskutoff NM, Betteridge KJ. 1992. The protective action of betaine on the metabolism of glucose and glutamine by cattle embryos produced and co-culture in vitro. J ReprodFertil. 95:585-595. Roberts, SJ. 1986. Veterinary obstetric and genital diseases (Theriogenology). 3 ed. Beltsville, EE UU. p. 488-513 Rodriguez-Martinez, H., Larsson, B., Pertoft, H. 1997. Evaluation of sperm damage and techniques for sperm clean-up. Reprod. Fertil. Dev. Vol. 9, pp. 297-308. Rossi CR, Bridgman BS, y Kiesel GK. 1990. Viral contamination of bovine fetal lung cultures and bovine fetal serum. Am. J. Vet. Res. 41:1680-1681. Ruiz, J, Correa, JE, & Martínez, M. 2010. Vitrification of bovine oocytes and its use in the parthenogenetic development of embryos. Archivos de medicina veterinaria, 42(1), 79-83. https://dx.doi.org/10.4067/S0301- 732X2010000100011 Saha, S.; Otoi, T; Takagi, M.; Boediono, A.; Sumantri, C.; Suzuki, T 1996. Normal calves obtained after direct transfer of vitrified bovine embryos using ethylene glycol, trehalosa, and polyvinylpyrrol¡done. Cryobiology 33:291-299. Saito, N.; Imai, K.; Tomizawa, M. 1994. Effect of sugars addition on the survival of vitrified bovine blastocysts produced in vitro. Theriogenology 41:1053-1060. Salisbury, GW; Van Demark, NL; Lodge, JR. 1982. Fisiología de la reproducción e inseminación artificial de los bóvidos. 2 ed. Zaragoza, España. Acribia. p. 419-565 Sánchez, R., Risopatron, J., Sepulveda, G., Peña, P., Miska, W. 1995. Evaluation of the acrosomal membrane in bovine spermatozoa effects of proteinase inhibitors. Theriogenology. Vol. 43, pp. 761-768. Shamsuddin M. 1993. In vitro Fertilization In The Bovine. PhD Thesis. Swedish University of Agricultural Sciences. Faculty of Veterinary Medicine. Sport, L; Carpenter, B; Thrift,T. 2009. Evaluación de la capacidad reproductiva de sementales Bos indicus y sus cruces en diferentes épocas del año en Tuzantla, Michoaclan. Tesina. Mexico. Universidad de Michoacana de san nicolas de Hidalgo. p. 49-70 Steeves TE, Gardner DK. 1999. Temporal and differential effects of amino acids on bovine embryo development in culture. Biol Reprod61;731-740. Stubbings, R. B., Wosik, G. P.1991. Glass wool versus swim-up separation of bovine spermatozoa for in vitro fertilization. Theriogenology. Vol. 35 pp. 276. TakahashiY, First NL. 1992. In vitro development of bovine one-cell embryos; influence of glucose, lactate, pyruvate, amino acids and vitamins. Theriogenology 37:963-978. Tea, N. T., Jondet, M., Scholler, R. 1983. Procéde d'isilement des spermatozoides mobiles de sperme humain per la méthode de migrationsédimentation. Pathol. Biol. Vol. 31, pp. 688-690. Tervit HR, Whittingham DG, Rowson, LEA. 1972. Successful culture in vitro of sheep andcattle ova. J ReprodFert; 30:493-497. Urrego R., Tarazona A., Olivera M., Camargo, O. 2008. Simplificación de la fertilización de ovocitos durante la producción in vitro de embriones bovinos. Rev Colomb Cienc Pecu; 21:398-405 Utsumi K., Kato, H., Iritani, A. 1981. Full-term development of bovine follicular oocytes matured in culture and fertilized in vitro. Theriogenology. Vol. 35, pp. 695-703. UTSUMI, K. L. Vajta, G.; Holm, P; Kuwayama, M.; Booth, P J.; Jacobsen, H.; Greve, T; Callesen, H. 1998. Open Pulled Straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol. Reprod. Dev. 51:53-58. Xu, K.P., H.C. Greve, y P. Hyttel. 1987. Co-culture of granulose cells with or without estrogens .J. Reprod. Fertil. 81: 501-504. Vajta, G.; Rindom, N.; Peura, T; Holm, P; Greve, T; Callensen, H. 1999. The effect of media, serum and temperature on in vitro suvival of bovine blastocysts after open pulled straw (OPS) vitrification. Theriogenology 52:939-948. Vera Muñoz, O. 2001. Técnicas de laboratorio para la evaluación del semental bovino. Seminario Internacional sobre biotecnología y patología reproductiva del bovino (1, 2001, Maracay, VE). Maracay, VE. IRAIA, FCV, UCV. p. 504-508 Vera, O; Bastidas, P. 1999. Análisis cromosómico y seminal de sementales Brahman de la Estación Experimental La Cumaca. Congreso Nacional de Ciencias Veterinarias. (4, 1999, Maracaibo,VE). Maracaibo, VE. p. 505-509 Wowk, B.; Leitl, E.; Rasch, C. M; Mesbah-Karimi, N.; Harris, S.; Fahy, G. M. 2000. Vitrification enhancement by synthetic ice blocking agents. Cryobiology 40:228-236 Yanagimachi, R. 1981. Mechanisms of fertilization in mammals. En fertilization and embryonic development in vitro. Plenum Press, New York, NY, pp. 81-182. Yang BK, Yang X, Foote RH. 1993. Effect of growth factors on development of IVM/IVF bovine embryos. Theriogenology 39:343. |
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Porras Vargas, José Luis0066faef4e5a86bf874e13d51472420b-1Gómez Oquendo, Jorge8151a7abd0b66237529143a57720abfe-1Maldonado Castro, Gerardo Antonio845ff215ffdaa212f3245c47c7237d0a-12018-12-12T14:29:57Z2018-12-12T14:29:57Z2018Maldonado Castro, G. A. (2018). Pasantía en el Laboratorio de Biotecnología Animal de la Facultad De Ciencias Agrarias del Politécnico Colombiano Jaime Isaza Cadavid. (Trabajo de pregrado). Universidad Pedagógica y Tecnológica de Colombia, Tunja. http://repositorio.uptc.edu.co/handle/001/2336http://repositorio.uptc.edu.co/handle/001/23361 recurso en línea (79 páginas) : ilustraciones color, tablas, figuras.La realización de la pasantía en el laboratorio de biotecnología animal del Politécnico Colombiano Jaime Isaza Cadavid permitió conocer, aprender y tomar destreza en los procesos de biotecnología reproductiva que se llevan a cabo en este lugar, tales como inseminación artificial, criopreservación de semen, sexado de espermatozoides, súper ovulación, transferencia de embriones, fertilización in vitro (FIV) y clonación, son usadas para mejorar la eficiencia reproductiva.Bibliografía y webgrafía: páginas 71-79.PregradoMédico Veterinario Zootecnistaapplication/pdfspaUniversidad Pedagógica y Tecnológica de ColombiaEscuela de Medicina Veterinaria y ZootecniaFacultad de Ciencias AgropecuariasCopyright (c) 2018 Universidad Pedagógica y Tecnológica de Colombiahttps://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2Pasantía en el Laboratorio de Biotecnología Animal de la Facultad De Ciencias Agrarias del Politécnico Colombiano Jaime Isaza CadavidTrabajo de grado - Pregradohttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/publishedVersionTexthttps://purl.org/redcol/resource_type/TPhttp://purl.org/coar/version/c_970fb48d4fbd8a85Avery, B., Greve, T. 1995. Impact of percollâ on bovine spermatozoa usedfor in vitro insemination. Theriogenology. Vol. 44, pp.871-878.Ball GD, Coulan CB, FieldCS, Harms RW, Thie JT, Byers AP. 1985. Effects of serumsource on human fertilization and embryonic growth parameters in vitro. Fertil Steril. 44:75-79.Bavister BD. 1992. Co-culture forembryo development: is it really necessary? Human Reproduction: 10:1339-1341.Brackett, B. D., Oliphant, G. 1975. Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. Vol. 12, pp. 260-274.Báez Contreras, Francisco J, Chávez Corona, Adeymi C, Hernández Fonseca, Hugo J, & Villamediana Monreal, Patricia C. 2010. Evaluation of in vitro development capacity of bovine oocytes obtained from predominantly Bos taurus and Bos indicus cows. Revista Científica, 20(3), 259-267. Recuperado, febrero de 2018, de http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0798- 22592010000300007&lng=es&tlng=enCabodevila J., Teruel M,. 2001. Criopreservación de embriones bovinos. En: Biotecnología de la reproducción. (ed.) Instituto Nacional de Tecnología Agropecuaria INTA. Argentina, pp. 149-174Cabrera P., Fernández A. 2006. Criopreservación de Embriones: una herramienta básica en la Reproducción Asistida. Revista de la Facultad de Ciencias Veterinaria Central de Venezuela 2009. Venezuela. Publicado en Crio protectores. Publicado en internet, disponible en Revista de la Facultad de Ciencias Veterinarias. Universidad Central de Venezuela.Catena, M; Cabodevila, J. 1999. Evaluación del semen bovino.Simposio Internacional de Reproduccion Bovina (UNCPBA).Tandil. Taurus. 1(3):18-31. Recuperado, febrero de 2018, de http://www2.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0258- 65762006000200001&lng=es&nrm=iso Activo agosto 2009Colenbrander, B.; Gadella, B.; Stout, T. 2003. The predictive value of semen analysis in the evaluation of the stallion fertility. Reprod. Dom. Anim. 38(4):305- 311.Crespo J.L y Guamán E. G. 2015. Fertilización in vitro en bovinos en el Laboratorio de Reproducción Animal de Zamorano utilizando el protocolo de Genes Difusión. Tesis de pregrado en Ingeniería Agronómica. Escuela Agrícola Panamericana, Zamorano Honduras.Cruz P, Medina V, Robles, Velasco Y. 2006 Evaluación de diferentes crioprotectores para la crioconservación de espermatozoides de yamú (Bryconamazonicus). Revista Colombiana de Ciencias Pecuarias. 2006; 19(2):153-159.Eagle H. 1959. Amino acid metabolis minmammalian cells. Science 130:432- 437.FAO. 2012. Ganadería Mundial 2011. La ganadería en la seguridad alimentaria (en línea). Roma, FAO. Recuperado, febrero de 2018; Disponible en: http://www.fao.org/docrep/016/i2373s/i2373s00.pdfFernández, Adriana, Díaz, Thaís, & Muñoz, Gladys. 2007. Producción In vitro de Embriones Bovinos. Revista de la Facultad de Ciencias Veterinarias, 48(1), 51-60. Recuperado en 03 de febrero de 2018, de http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0258- 65762007000100006&lng=es&tlng=es.Fry, R. C., Niall, E. M., Simpson, T. L., Squires, T. J., Reynolds, J. 1997. The collection of oocytes from bovine ovaries. Theriogenology. Vol. 47, pp. 977-987.Fukuda, Y., Ichikawa, M., Naito, K., Toyoda, Y. 1990. Birth of normal calves resulting from bovine oocytes matured, fertilized, and cultured with cumulus cells in vitro up to blastocyst stage. Biol. Reprod. Vol. 42, pp. 114-119.GandolfiF, BreviniTA, MoorRM. 1989. Effect of oviduct environment on embryonic development. J ReprodFert. 38:107-115.Gardner, HG, Kaye PL. 1991. Insulinincreases cell numbers andmorphological development inmouse pre-implantationembryos in vitro. ReprodFert Dev 3:79- 91.Geshi, M., Takenouchi, N., Yameuchi,N., Nagai,T. 2000. 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Relationship of morphology and follicular fluid environment of oocytes to their developmental potential in vitro.Theriogenology, 43:502-509.Hidalgo, C; Tamargo, C; Diez, C. 2005. Análisis del semen bovino. Asturias, España. Información ganadera. 39 Boletín Informativo SERIDA. No. 2. p. 39-43Hofig, A, Simmen FA, Simmen RCM. 1990. Effects of dihydroepiandrosterone (DHEA), insulin-like growthfactor(IGF-1), Insulin(I) onestradiol productionby porcine conceptuses in vitro. Biol Reprod. 42 (Suppl. 1), 135.Hyttel, P., Xu, K. P., Smith, S., Greve, T. 1986. Ultrastructure of in vitro oocyte maturation in cattle.J. Reprod. Fert. Vol. 78, pp. 615-625. Recuperado, febrero de 2018, de http://www.monografias.com/trabajos903/vitrificacion tecnicacrioconservacion/vitrificacion-tecnica-crioconservacion2.shtmlKastelic JP, Cook RB, Pierson RA, Coulter GH. Relationships among scrotal and testicular characteristics, sperm production, and seminal quality in 129 beef bulls. 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Defined media for vitrification, warming, and rehydration: effects on post-thaw protein synthesis and viability of in vitro derived ovine embryos. Cryobiology 45:204-2012.Lessley, B. A., Garner, D. L. 1983. Isolation of motile spermatozoa by density radient centrifugation in percoll. Gamate Res. Vol. 7, pp. 49-65.Lonergan, P.,Rizos,D., Kanka,L., Nemcova,L., Mbaye,AM., Kingston,M., Wade,M., Duffy,P., Boland,M. 2003. Temporal sensitivity of bovine embryos to culture environment after fertilization and the implications for blastocyst quality. Reproduction 126,337–346.Lonergan,P.,Vergos, E., Kinis,A.,Sharif,H., Gordon,I. 1991. The effect of recovery method o the type of bovine oocytes obtained for IVM.Theriogenology,35:231.Lonergan, P.,Rizos,D., Kanka,L., Nemcova,L., Mbaye,AM., Kingston,M., Wade,M., Duffy,P., Boland,M. 2003. Temporal sensitivity of bovine embryos to culture environment after fertilization and the implications for blastocyst quality. ReproductionLu, K. H., Gordon, I., Gallager, M., Mcgovern, H. 1987. Pregnancy established in cattle by transfer of embryos derived from in vitro fertilization of oocytes matured in vitro. Vet. Rec. Vol. 121, pp. 259-260.Madan, M.L. 2005. Animal biotechnology: applications and economic implication in developing countries. Revue Scientifique et Technique Office International Des Epizooties 24(1): 123-139.Marguant-Le Guienne B, Gerard M, Solari A y Thibault C. 1989. In vitro culture of bovine eggs fertilized eitherinvivo or in vitro. ReprodNutrDev 29:559-568.Massip, A.; Van Der Zwalmen, P; Ecotrs, E. 1987. Recent progress in cryopreservation of cattle embryos. Theriogenology 27:69-79Morillo, M; Salazar, S; Castillo, E. 2012. Evaluación del potencial reproductivo del macho bovino. Maracay, VE, Instituto Nacional de Investigaciones Agrícolas. 60 p. Centro Nacional de Investigaciones AgropecuariasNiwa, K., Ohoda, O. 1988. 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Theriogenology 39:343.Mejoramiento genéticoObstetricia veterinariaReproducción animalEmbriologíaTransplante de embrionesInseminación artificialInseminaciónMedicina Veterinaria y Zootecnia - Tesis y disertaciones académicasAgrosaviaORIGINALTGT-956.pdfTGT-956.pdfArchivo principalapplication/pdf1318452https://repositorio.uptc.edu.co/bitstreams/d0a593e7-1d7d-4055-94fd-d4044f858167/download4165aefef78930c4437e5a4872c26321MD51A_GAMC.pdfA_GAMC.pdfAutorización publicaciónapplication/pdf661516https://repositorio.uptc.edu.co/bitstreams/cfa492da-1810-49d3-9770-14766d0facea/downloadebadd01a106a88c267872cd1aa4ea05dMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-814798https://repositorio.uptc.edu.co/bitstreams/59a378fe-654e-41ea-b72a-3b72e7b631aa/download88794144ff048353b359a3174871b0d5MD53TEXTTGT-956.pdf.txtTGT-956.pdf.txtExtracted 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