Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L.

This study aimed at isolating diazotrophic bacteria with promoting activity of vegetal growth, associated to carrot culture. Samples were taken at 30, 60 and 115 days in a farm located in a rural area of the municipality of Manizales (Caldas). The diazotrophic bacteria were isolated in semi-solid cu...

Full description

Autores:
Tipo de recurso:
Fecha de publicación:
2018
Institución:
Universidad Pedagógica y Tecnológica de Colombia
Repositorio:
RiUPTC: Repositorio Institucional UPTC
Idioma:
spa
OAI Identifier:
oai:repositorio.uptc.edu.co:001/10600
Acceso en línea:
https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7753
https://repositorio.uptc.edu.co/handle/001/10600
Palabra clave:
carrot
nitrogen fixation
nitrogenase activity
phosphate solubilization
actividad nitrogenasa
fijación de nitrógeno
solubilización de fosfato
zanahoria
Rights
License
http://purl.org/coar/access_right/c_abf2
id REPOUPTC2_0b32846910087634c7154e1b7777d325
oai_identifier_str oai:repositorio.uptc.edu.co:001/10600
network_acronym_str REPOUPTC2
network_name_str RiUPTC: Repositorio Institucional UPTC
repository_id_str
dc.title.en-US.fl_str_mv Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L.
dc.title.es-ES.fl_str_mv Bacterias diazotróficas con actividad promotora del crecimiento vegetal en Daucus carota L.
title Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L.
spellingShingle Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L.
carrot
nitrogen fixation
nitrogenase activity
phosphate solubilization
actividad nitrogenasa
fijación de nitrógeno
solubilización de fosfato
zanahoria
title_short Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L.
title_full Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L.
title_fullStr Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L.
title_full_unstemmed Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L.
title_sort Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L.
dc.subject.en-US.fl_str_mv carrot
nitrogen fixation
nitrogenase activity
phosphate solubilization
topic carrot
nitrogen fixation
nitrogenase activity
phosphate solubilization
actividad nitrogenasa
fijación de nitrógeno
solubilización de fosfato
zanahoria
dc.subject.es-ES.fl_str_mv actividad nitrogenasa
fijación de nitrógeno
solubilización de fosfato
zanahoria
description This study aimed at isolating diazotrophic bacteria with promoting activity of vegetal growth, associated to carrot culture. Samples were taken at 30, 60 and 115 days in a farm located in a rural area of the municipality of Manizales (Caldas). The diazotrophic bacteria were isolated in semi-solid culture media free of nitrogen. We described the macro and microscopic features of the obtained isolations, and identified them biochemically and molecularly. In addition, we evaluated indole compounds, nitrogenase activity and solubilization of phosphates. Gluconacetobacter diazotrophicus ATCC 49037 was used as a standard strain. We recovered 20 isolates associated to rhizosphere and 12 to rhizoplane. The molecular identification showed the presence of five genera: Rhizobium, Achromobacter, Bacillus, Enterobacter, and Stenotrophomonas. The production of indole compounds presented concentrations between 9.73 and 112.8 μg/mL. The standard strain showed a higher activity, with an indolic compound production of 172.5 μg/mL. Regarding nitrogenase activity, the isolates GIBI411, 394 and 399 had an activity greater or similar to the standard strain. The most efficient isolates in the solubilization of tricalcium phosphate were GIBI378 and 385. The solubilization of aluminum phosphate was assessed by the acid production index, with the isolates GIBI378, 391, 387 and 388 presenting the best behavior. The isolates found are potential candidates for the development of novel biotechnological processes to produce new alternative biofertilizers, considering the significant plant-growth promotion properties determined in this work.
publishDate 2018
dc.date.accessioned.none.fl_str_mv 2024-07-05T18:11:28Z
dc.date.available.none.fl_str_mv 2024-07-05T18:11:28Z
dc.date.none.fl_str_mv 2018-02-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
dc.type.en-US.fl_str_mv research
dc.type.es-ES.fl_str_mv investigación
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.identifier.none.fl_str_mv https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7753
10.19053/01228420.v15.n1.2018.7753
dc.identifier.uri.none.fl_str_mv https://repositorio.uptc.edu.co/handle/001/10600
url https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7753
https://repositorio.uptc.edu.co/handle/001/10600
identifier_str_mv 10.19053/01228420.v15.n1.2018.7753
dc.language.none.fl_str_mv spa
dc.language.iso.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7753/6235
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv http://purl.org/coar/access_right/c_abf2
dc.format.none.fl_str_mv application/pdf
dc.publisher.en-US.fl_str_mv Universidad Pedagógica y Tecnológica de Colombia
dc.source.en-US.fl_str_mv Ciencia y Agricultura; Vol. 15 No. 1 (2018); 19-27
dc.source.es-ES.fl_str_mv Ciencia y Agricultura; Vol. 15 Núm. 1 (2018); 19-27
dc.source.none.fl_str_mv 2539-0899
institution Universidad Pedagógica y Tecnológica de Colombia
repository.name.fl_str_mv Repositorio Institucional UPTC
repository.mail.fl_str_mv repositorio.uptc@uptc.edu.co
_version_ 1839633838438875136
spelling 2018-02-012024-07-05T18:11:28Z2024-07-05T18:11:28Zhttps://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/775310.19053/01228420.v15.n1.2018.7753https://repositorio.uptc.edu.co/handle/001/10600This study aimed at isolating diazotrophic bacteria with promoting activity of vegetal growth, associated to carrot culture. Samples were taken at 30, 60 and 115 days in a farm located in a rural area of the municipality of Manizales (Caldas). The diazotrophic bacteria were isolated in semi-solid culture media free of nitrogen. We described the macro and microscopic features of the obtained isolations, and identified them biochemically and molecularly. In addition, we evaluated indole compounds, nitrogenase activity and solubilization of phosphates. Gluconacetobacter diazotrophicus ATCC 49037 was used as a standard strain. We recovered 20 isolates associated to rhizosphere and 12 to rhizoplane. The molecular identification showed the presence of five genera: Rhizobium, Achromobacter, Bacillus, Enterobacter, and Stenotrophomonas. The production of indole compounds presented concentrations between 9.73 and 112.8 μg/mL. The standard strain showed a higher activity, with an indolic compound production of 172.5 μg/mL. Regarding nitrogenase activity, the isolates GIBI411, 394 and 399 had an activity greater or similar to the standard strain. The most efficient isolates in the solubilization of tricalcium phosphate were GIBI378 and 385. The solubilization of aluminum phosphate was assessed by the acid production index, with the isolates GIBI378, 391, 387 and 388 presenting the best behavior. The isolates found are potential candidates for the development of novel biotechnological processes to produce new alternative biofertilizers, considering the significant plant-growth promotion properties determined in this work.Este trabajo se propuso aislar bacterias diazotróficas con actividad promotora del crecimiento vegetal, asociadas al cultivo de zanahoria. Se realizaron 3 muestreos, a los 30, 60 y 115 días, en una granja ubicada en una zona rural del municipio de Manizales (Caldas). El aislamiento de las bacterias diazotróficas se efectuó en medios de cultivo semisólidos libres de nitrógeno. A los aislamientos obtenidos se les realizó descripción macro y microscópica, identificación bioquímica y molecular. Se evaluaron características como determinación de compuestos indólicos, actividad nitrogenasa y solubilización de fosfatos. Como cepa patrón se empleó Gluconacetobacter diazotrophicus ATCC 49037. Se recuperaron 20 aislamientos asociados a la rizosfera y 12 al rizoplano. La identificación molecular mostró cinco géneros presentes: Rhizobium, Achromobacter, Bacillus, Enterobacter y Stenotrophomonas. La producción de compuestos indólicos presentó concentraciones entre 9,73 y 112,8 µg/mL. La cepa patrón presentó una actividad mayor, con una producción de compuestos indólicos de 172,5 µg/mL. En la actividad nitrogenasa los aislamientos GIBI411, 394 y 399 tuvieron una actividad mayor o similar a la cepa patrón. Los aislamientos más eficientes en la solubilización de fosfato tricálcico fueron GIBI378 y 385. La solubilización de fosfato de aluminio se valoró por el índice de producción de ácido, siendo los aislamientos GIBI378, 391, 387 y 388 los de mejor comportamiento en esta variable. Los aislamientos encontrados son candidatos potenciales para desarrollar nuevos procesos biotecnológicos para la producción de nuevos biofertilizantes alternativos, considerando las importantes propiedades de promoción del crecimiento vegetal determinadas en este trabajo.application/pdfspaspaUniversidad Pedagógica y Tecnológica de Colombiahttps://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7753/6235Ciencia y Agricultura; Vol. 15 No. 1 (2018); 19-27Ciencia y Agricultura; Vol. 15 Núm. 1 (2018); 19-272539-0899carrotnitrogen fixationnitrogenase activityphosphate solubilizationactividad nitrogenasafijación de nitrógenosolubilización de fosfatozanahoriaDiazotrophic bacteria with activity promoting vegetable growth in Daucus carota L.Bacterias diazotróficas con actividad promotora del crecimiento vegetal en Daucus carota L.info:eu-repo/semantics/articleresearchinvestigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/access_right/c_abf2Gaviria-Giraldo, JenniferRestrepo-Franco, Gloria MaríaGaleano-Vanegas, Narmer FernandoHernández-Rodríguez, Annia001/10600oai:repositorio.uptc.edu.co:001/106002025-07-18 11:01:23.381metadata.onlyhttps://repositorio.uptc.edu.coRepositorio Institucional UPTCrepositorio.uptc@uptc.edu.co

Publicaciones similares