An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
Histoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our knowledge,...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2019
- Institución:
- Universidad de Medellín
- Repositorio:
- Repositorio UDEM
- Idioma:
- eng
- OAI Identifier:
- oai:repository.udem.edu.co:11407/5766
- Acceso en línea:
- http://hdl.handle.net/11407/5766
- Palabra clave:
- Histoplasma capsulatum
histoplasmosis
interferon gamma release assay
latent infection
- Rights
- License
- http://purl.org/coar/access_right/c_16ec
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20192020-04-29T14:53:56Z2020-04-29T14:53:56Z14602709http://hdl.handle.net/11407/576610.1093/mmy/myy131Histoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our knowledge, we explore the suitability of an interferon gamma release assay (IGRA) to detect latent Hc infection in asymptomatic individuals. A cohort of 126 volunteers was enrolled in the study, 13 of which underwent a Hc infection in the past, and 93 of them showing risk factors for this infection. The remaining 20 participants did not refer any risk factors of Hc infection, but eight of them showed evidences of infection with Mycobacterium tuberculosis. All participants were recruited in Medellin, Colombia, between January 2014 and December 2017. Whole blood samples were cultured with four different Hc crude antigens and phytohemaglutinin as positive control. The interferon (IFN)-? released by T lymphocytes upon antigen stimulation was quantified by ELISA. A defined cutoff value of 20 pg/ml for the IFN-? concentration allowed us to distinguish between the group with documented past infections and the group of noninfected individuals with high sensitivity (70-92%) and specificity (85-95%), for the four tested antigens. Positive 82-95% and negative 77-92% predictive values were also very high, comparable to those reported for commercially available IGRAs. The new test constitutes a promising screening method to detect individuals with latent Hc infection, even decades after the primary infection, as evidenced in this study. © The Author(s) 2018. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.engNLM (Medline)Facultad de Ciencias BásicasFacultad de Ciencias Básicashttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85073288547&doi=10.1093%2fmmy%2fmyy131&partnerID=40&md5=d02c3c8a20a3b1332ce66f1efb638211576724732Medical mycologyHistoplasma capsulatumhistoplasmosisinterferon gamma release assaylatent infectionAn interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept studyArticleinfo:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Rubio-Carrasquilla, M., Grupo de Micología Médica y Experimental, Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia, Instituto de Biología, Universidad de Antioquia, Medellín, Colombia; Santa, C.D., Universidad Nacional de Colombia, Sede Medellín, Medellín, Colombia; Rendón, J.P., Grupo de Micología Médica y Experimental, Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia; Botero-Garcés, J., Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Guimarães, A.J., Depto de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói-RJ, Brazil; Moreno, E., Facultad de Ciencias Básicas, Universidad de Medellín, Medellín, Colombia; Cano, L.E., Grupo de Micología Médica y Experimental, Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia, Escuela de Microbiología, Universidad de Antioquia, Medellín, Colombiahttp://purl.org/coar/access_right/c_16ecRubio-Carrasquilla M.Santa C.D.Rendón J.P.Botero-Garcés J.Guimarães A.J.Moreno E.Cano L.E.11407/5766oai:repository.udem.edu.co:11407/57662020-05-27 19:01:51.45Repositorio Institucional Universidad de Medellinrepositorio@udem.edu.co |
dc.title.none.fl_str_mv |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study |
title |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study |
spellingShingle |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study Histoplasma capsulatum histoplasmosis interferon gamma release assay latent infection |
title_short |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study |
title_full |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study |
title_fullStr |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study |
title_full_unstemmed |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study |
title_sort |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study |
dc.subject.none.fl_str_mv |
Histoplasma capsulatum histoplasmosis interferon gamma release assay latent infection |
topic |
Histoplasma capsulatum histoplasmosis interferon gamma release assay latent infection |
description |
Histoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our knowledge, we explore the suitability of an interferon gamma release assay (IGRA) to detect latent Hc infection in asymptomatic individuals. A cohort of 126 volunteers was enrolled in the study, 13 of which underwent a Hc infection in the past, and 93 of them showing risk factors for this infection. The remaining 20 participants did not refer any risk factors of Hc infection, but eight of them showed evidences of infection with Mycobacterium tuberculosis. All participants were recruited in Medellin, Colombia, between January 2014 and December 2017. Whole blood samples were cultured with four different Hc crude antigens and phytohemaglutinin as positive control. The interferon (IFN)-? released by T lymphocytes upon antigen stimulation was quantified by ELISA. A defined cutoff value of 20 pg/ml for the IFN-? concentration allowed us to distinguish between the group with documented past infections and the group of noninfected individuals with high sensitivity (70-92%) and specificity (85-95%), for the four tested antigens. Positive 82-95% and negative 77-92% predictive values were also very high, comparable to those reported for commercially available IGRAs. The new test constitutes a promising screening method to detect individuals with latent Hc infection, even decades after the primary infection, as evidenced in this study. © The Author(s) 2018. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. |
publishDate |
2019 |
dc.date.accessioned.none.fl_str_mv |
2020-04-29T14:53:56Z |
dc.date.available.none.fl_str_mv |
2020-04-29T14:53:56Z |
dc.date.none.fl_str_mv |
2019 |
dc.type.eng.fl_str_mv |
Article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
dc.identifier.issn.none.fl_str_mv |
14602709 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/11407/5766 |
dc.identifier.doi.none.fl_str_mv |
10.1093/mmy/myy131 |
identifier_str_mv |
14602709 10.1093/mmy/myy131 |
url |
http://hdl.handle.net/11407/5766 |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.isversionof.none.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85073288547&doi=10.1093%2fmmy%2fmyy131&partnerID=40&md5=d02c3c8a20a3b1332ce66f1efb638211 |
dc.relation.citationvolume.none.fl_str_mv |
57 |
dc.relation.citationissue.none.fl_str_mv |
6 |
dc.relation.citationstartpage.none.fl_str_mv |
724 |
dc.relation.citationendpage.none.fl_str_mv |
732 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_16ec |
rights_invalid_str_mv |
http://purl.org/coar/access_right/c_16ec |
dc.publisher.none.fl_str_mv |
NLM (Medline) |
dc.publisher.program.none.fl_str_mv |
Facultad de Ciencias Básicas |
dc.publisher.faculty.none.fl_str_mv |
Facultad de Ciencias Básicas |
publisher.none.fl_str_mv |
NLM (Medline) |
dc.source.none.fl_str_mv |
Medical mycology |
institution |
Universidad de Medellín |
repository.name.fl_str_mv |
Repositorio Institucional Universidad de Medellin |
repository.mail.fl_str_mv |
repositorio@udem.edu.co |
_version_ |
1814159238907297792 |