An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study

Histoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our knowledge,...

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Tipo de recurso:
Fecha de publicación:
2019
Institución:
Universidad de Medellín
Repositorio:
Repositorio UDEM
Idioma:
eng
OAI Identifier:
oai:repository.udem.edu.co:11407/5766
Acceso en línea:
http://hdl.handle.net/11407/5766
Palabra clave:
Histoplasma capsulatum
histoplasmosis
interferon gamma release assay
latent infection
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http://purl.org/coar/access_right/c_16ec
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spelling 20192020-04-29T14:53:56Z2020-04-29T14:53:56Z14602709http://hdl.handle.net/11407/576610.1093/mmy/myy131Histoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our knowledge, we explore the suitability of an interferon gamma release assay (IGRA) to detect latent Hc infection in asymptomatic individuals. A cohort of 126 volunteers was enrolled in the study, 13 of which underwent a Hc infection in the past, and 93 of them showing risk factors for this infection. The remaining 20 participants did not refer any risk factors of Hc infection, but eight of them showed evidences of infection with Mycobacterium tuberculosis. All participants were recruited in Medellin, Colombia, between January 2014 and December 2017. Whole blood samples were cultured with four different Hc crude antigens and phytohemaglutinin as positive control. The interferon (IFN)-? released by T lymphocytes upon antigen stimulation was quantified by ELISA. A defined cutoff value of 20 pg/ml for the IFN-? concentration allowed us to distinguish between the group with documented past infections and the group of noninfected individuals with high sensitivity (70-92%) and specificity (85-95%), for the four tested antigens. Positive 82-95% and negative 77-92% predictive values were also very high, comparable to those reported for commercially available IGRAs. The new test constitutes a promising screening method to detect individuals with latent Hc infection, even decades after the primary infection, as evidenced in this study. © The Author(s) 2018. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.engNLM (Medline)Facultad de Ciencias BásicasFacultad de Ciencias Básicashttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85073288547&doi=10.1093%2fmmy%2fmyy131&partnerID=40&md5=d02c3c8a20a3b1332ce66f1efb638211576724732Medical mycologyHistoplasma capsulatumhistoplasmosisinterferon gamma release assaylatent infectionAn interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept studyArticleinfo:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Rubio-Carrasquilla, M., Grupo de Micología Médica y Experimental, Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia, Instituto de Biología, Universidad de Antioquia, Medellín, Colombia; Santa, C.D., Universidad Nacional de Colombia, Sede Medellín, Medellín, Colombia; Rendón, J.P., Grupo de Micología Médica y Experimental, Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia; Botero-Garcés, J., Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Guimarães, A.J., Depto de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói-RJ, Brazil; Moreno, E., Facultad de Ciencias Básicas, Universidad de Medellín, Medellín, Colombia; Cano, L.E., Grupo de Micología Médica y Experimental, Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia, Escuela de Microbiología, Universidad de Antioquia, Medellín, Colombiahttp://purl.org/coar/access_right/c_16ecRubio-Carrasquilla M.Santa C.D.Rendón J.P.Botero-Garcés J.Guimarães A.J.Moreno E.Cano L.E.11407/5766oai:repository.udem.edu.co:11407/57662020-05-27 19:01:51.45Repositorio Institucional Universidad de Medellinrepositorio@udem.edu.co
dc.title.none.fl_str_mv An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
title An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
spellingShingle An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
Histoplasma capsulatum
histoplasmosis
interferon gamma release assay
latent infection
title_short An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
title_full An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
title_fullStr An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
title_full_unstemmed An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
title_sort An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
dc.subject.none.fl_str_mv Histoplasma capsulatum
histoplasmosis
interferon gamma release assay
latent infection
topic Histoplasma capsulatum
histoplasmosis
interferon gamma release assay
latent infection
description Histoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our knowledge, we explore the suitability of an interferon gamma release assay (IGRA) to detect latent Hc infection in asymptomatic individuals. A cohort of 126 volunteers was enrolled in the study, 13 of which underwent a Hc infection in the past, and 93 of them showing risk factors for this infection. The remaining 20 participants did not refer any risk factors of Hc infection, but eight of them showed evidences of infection with Mycobacterium tuberculosis. All participants were recruited in Medellin, Colombia, between January 2014 and December 2017. Whole blood samples were cultured with four different Hc crude antigens and phytohemaglutinin as positive control. The interferon (IFN)-? released by T lymphocytes upon antigen stimulation was quantified by ELISA. A defined cutoff value of 20 pg/ml for the IFN-? concentration allowed us to distinguish between the group with documented past infections and the group of noninfected individuals with high sensitivity (70-92%) and specificity (85-95%), for the four tested antigens. Positive 82-95% and negative 77-92% predictive values were also very high, comparable to those reported for commercially available IGRAs. The new test constitutes a promising screening method to detect individuals with latent Hc infection, even decades after the primary infection, as evidenced in this study. © The Author(s) 2018. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.
publishDate 2019
dc.date.accessioned.none.fl_str_mv 2020-04-29T14:53:56Z
dc.date.available.none.fl_str_mv 2020-04-29T14:53:56Z
dc.date.none.fl_str_mv 2019
dc.type.eng.fl_str_mv Article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.identifier.issn.none.fl_str_mv 14602709
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/11407/5766
dc.identifier.doi.none.fl_str_mv 10.1093/mmy/myy131
identifier_str_mv 14602709
10.1093/mmy/myy131
url http://hdl.handle.net/11407/5766
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.isversionof.none.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-85073288547&doi=10.1093%2fmmy%2fmyy131&partnerID=40&md5=d02c3c8a20a3b1332ce66f1efb638211
dc.relation.citationvolume.none.fl_str_mv 57
dc.relation.citationissue.none.fl_str_mv 6
dc.relation.citationstartpage.none.fl_str_mv 724
dc.relation.citationendpage.none.fl_str_mv 732
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_16ec
rights_invalid_str_mv http://purl.org/coar/access_right/c_16ec
dc.publisher.none.fl_str_mv NLM (Medline)
dc.publisher.program.none.fl_str_mv Facultad de Ciencias Básicas
dc.publisher.faculty.none.fl_str_mv Facultad de Ciencias Básicas
publisher.none.fl_str_mv NLM (Medline)
dc.source.none.fl_str_mv Medical mycology
institution Universidad de Medellín
repository.name.fl_str_mv Repositorio Institucional Universidad de Medellin
repository.mail.fl_str_mv repositorio@udem.edu.co
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